Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,056 +T 8.1% intergenic (+104/‑138) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0561.→T 8.1% 35.2 ‑0.3 ‑1.0123intergenic (+104/‑138)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/4):  ref base (61/52):  total (67/56)
Fisher's exact test strand distribution p-value = 7.54e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e-01

Only 100 of 131 total aligned reads displayed.

GGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAG..AAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAG  >  NC_014034/2493956‑2494151
                                                                                                     |                                                                                                       
ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGaa                                                                                                          <  1:3964032/101‑1 (MQ=255)
 gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                                                        >  1:2855095/1‑101 (MQ=255)
 gcgcccTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                                                        <  2:915472/96‑1 (MQ=255)
  ggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                                                                       >  1:1980829/1‑101 (MQ=255)
       tccccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aa                                                                                               <  1:1852439/99‑2 (MQ=255)
       tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGCA.TGGC..G.aa                                                                                               >  1:123812/1‑100 (MQ=255)
       tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aa                                                                                               >  1:3321008/1‑100 (MQ=255)
        gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                                                              >  1:301015/1‑99 (MQ=255)
        gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aag                                                                                              >  1:703809/1‑99 (MQ=255)
         cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                                                            <  1:157847/101‑5 (MQ=255)
          ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aagcca                                                                                           >  1:3490525/1‑96 (MQ=18)
            cccgtCCGCAATCTGACTCCCCCCGTGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                                                          <  1:713144/97‑7 (MQ=18)
            cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                                                          <  1:1478641/97‑7 (MQ=255)
             ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                                                                         >  2:3182049/4‑94 (MQ=255)
              cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagag                                                                                        <  1:4031167/99‑9 (MQ=255)
               gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagagg                                                                                       <  2:4026053/100‑10 (MQ=255)
               gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagagg                                                                                       >  2:1266907/2‑92 (MQ=255)
                     caGCGGTCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCa                                                                               <  2:1690685/100‑1 (MQ=18)
                     aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCa                                                                               <  1:1217599/101‑1 (MQ=18)
                     aaGCGCACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCa                                                                               <  1:2125088/101‑1 (MQ=18)
                      aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCag                                                                              <  2:1980703/101‑2 (MQ=18)
                        cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCagcg                                                                            >  1:262851/1‑98 (MQ=18)
                         ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCagcgc                                                                           >  2:4233939/1‑97 (MQ=18)
                         ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAGGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCaccgc                                                                           >  1:1861491/1‑97 (MQ=17)
                          gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.cc                                                                        >  2:3495375/1‑101 (MQ=17)
                           aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.ccc                                                                       >  2:181846/1‑101 (MQ=17)
                            cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.cccc                                                                      >  2:231394/1‑101 (MQ=18)
                            cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.cccc                                                                      <  1:2553565/101‑1 (MQ=17)
                            cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.cccc                                                                      <  2:1471921/101‑1 (MQ=17)
                             tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.ccccc                                                                     >  2:321368/1‑101 (MQ=18)
                             tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.ccccc                                                                     >  2:633975/1‑101 (MQ=17)
                               cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.cccccct                                                                   <  1:4113550/101‑3 (MQ=17)
                               cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.cccccct                                                                   <  2:640139/101‑3 (MQ=17)
                               cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.cccccct                                                                   <  2:936538/101‑3 (MQ=17)
                               cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.acccccg                                                                   >  2:2285455/1‑99 (MQ=17)
                                   cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGA..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGccc                                                                <  1:3748899/101‑1 (MQ=17)
                                     ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCcg                                                              <  2:1524554/101‑1 (MQ=17)
                                     ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCcg                                                              >  1:1411281/1‑101 (MQ=17)
                                      gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgc                                                             <  1:76569/101‑2 (MQ=0)
                                      gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCcgg                                                             >  2:3035939/1‑101 (MQ=18)
                                       atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgcc                                                            >  2:2911175/1‑99 (MQ=2)
                                       atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgcc                                                            >  1:1063102/1‑99 (MQ=2)
                                       atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgcc                                                            <  1:2038261/101‑3 (MQ=2)
                                       atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCcggc                                                            >  2:2615727/1‑101 (MQ=18)
                                       atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCA..GAGGGGGGC.CGCGCCCCCCCGCCCcggc                                                            >  1:1983187/1‑101 (MQ=14)
                                         atTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGC..C.AA.ACGCCAAGAGGGGGAAGCG.CCCCCTGCCCcgccgg                                                          >  1:3675887/1‑101 (MQ=2)
                                         atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCcggcgg                                                          >  2:3916301/1‑101 (MQ=18)
                                           ttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgccggat                                                        <  1:582313/101‑3 (MQ=2)
                                           ttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgccggat                                                        <  2:3744366/101‑3 (MQ=2)
                                            tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTcc                                                       >  1:3235319/1‑101 (MQ=17)
                                             gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCATCCC.CCCCCTGCCCCGGCGGTCCg                                                      <  2:3138149/101‑1 (MQ=18)
                                             gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCg                                                      <  1:3695775/101‑1 (MQ=17)
                                               agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTg                                                    <  2:1717607/101‑1 (MQ=17)
                                               agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATAGCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTg                                                    <  1:181884/101‑1 (MQ=17)
                                                gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTgg                                                   <  2:1059460/101‑1 (MQ=17)
                                                 caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGGCCGTggg                                                  >  1:143678/1‑100 (MQ=17)
                                                   aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcgg                                                <  1:2538613/101‑1 (MQ=17)
                                                   aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcgg                                                >  1:4224779/1‑101 (MQ=17)
                                                     aTGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCAGCTC.CCCCCTGCCCCGGCGGTCCGTGgcggcg                                              >  1:2845295/1‑101 (MQ=18)
                                                        aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGGGCCt                                           >  2:911065/1‑101 (MQ=17)
                                                        aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGGCCGGGGCGGCGGCt                                           >  1:2207444/1‑101 (MQ=9)
                                                          aCCGGATGCCTTCCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCAGGCCGTGGGGGGGCCTTg                                         >  1:932663/1‑101 (MQ=9)
                                                           ccGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGa                                        >  1:2841566/1‑101 (MQ=17)
                                                            cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                       <  1:882183/101‑1 (MQ=17)
                                                            cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCA..GAGGGGGGCAGGGC.CCCCCTGCCCCGGCGGGCCGCGGCGGCGCCCTGAc                                       >  1:3622924/1‑101 (MQ=17)
                                                              gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc.gg                                    <  2:2298375/101‑1 (MQ=17)
                                                               aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc.ggg                                   <  1:4122895/101‑1 (MQ=17)
                                                                tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc.gggc                                  >  2:517161/1‑101 (MQ=17)
                                                                 gCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc.gggcg                                 <  2:2046365/101‑1 (MQ=17)
                                                                  ccTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc.gggcgg                                <  1:1165216/101‑1 (MQ=17)
                                                                   cTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc.gggcggg                               >  2:3396601/1‑101 (MQ=18)
                                                                    tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCAGGGG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCTGTGAC.GGGCgggg                              >  2:3060860/1‑101 (MQ=18)
                                                                    tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCgggg                              >  2:3468148/1‑101 (MQ=18)
                                                                     ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGCGGCGGCGCCTTGAC.GGGCggggc                             >  2:3271940/1‑100 (MQ=17)
                                                                      tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGgc                            >  2:1293961/1‑101 (MQ=18)
                                                                      tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGgc                            >  2:1319747/1‑101 (MQ=18)
                                                                         tCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGGC.GGGCGGGGGcgcg                         >  2:1136849/1‑101 (MQ=18)
                                                                           ttGCCAGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGGCTTGAC.GGGCGGGGGGGCggg                       >  1:43816/1‑101 (MQ=17)
                                                                            tGCCCGAAATATGCGGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGc                      <  2:2602539/101‑1 (MQ=18)
                                                                              cccGAAATATCCCGGGGGGTGAA.TGAC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCAt                    >  2:65134/1‑101 (MQ=18)
                                                                               ccGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCAtt                   <  1:321413/101‑1 (MQ=18)
                                                                                cGAAATTTCCCGGGGGGGTAA.TGGC..G.AA.GCC..AGGGGGGGGCAGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCACTa                  <  1:2414389/101‑1 (MQ=16)
                                                                                cGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTa                  <  2:2982465/101‑1 (MQ=18)
                                                                                cGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTa                  <  2:262806/101‑1 (MQ=18)
                                                                                 gAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTaa                 <  2:3159051/101‑1 (MQ=18)
                                                                                  aagtgtCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAAc                <  1:3190945/96‑1 (MQ=18)
                                                                                     tatCCGGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGa             <  2:1519688/101‑1 (MQ=21)
                                                                                     tatCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGa             >  1:3055170/1‑101 (MQ=21)
                                                                                     tatCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGa             >  2:1783617/1‑101 (MQ=21)
                                                                                      atCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGG.GAGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTCGAC.GGGGGGGGGCGCGGGCAGTAACCCaa            >  1:640583/1‑101 (MQ=12)
                                                                                       tCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGaaa           >  2:2713553/1‑101 (MQ=21)
                                                                                        cccGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAAt          <  2:2775235/101‑1 (MQ=21)
                                                                                          cGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTg        >  2:1127724/1‑101 (MQ=25)
                                                                                           ggggggTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGc       <  1:4170531/101‑1 (MQ=25)
                                                                                           ggggggTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGAGGTCCGAGGGGGGGCCTTGACTGGGCGGGGG.GCGGGCGTTAACGGaagtggc       >  1:1608576/1‑96 (MQ=2)
                                                                                           ggggggCGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCCTTAACCGAAATTGc       >  2:1282704/1‑101 (MQ=21)
                                                                                            gggggTGAA.TGGC..GAAGCCAG...AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGCa      >  2:4162475/1‑101 (MQ=25)
                                                                                            gggggTGAA.AGGC..GAAG.CCA..GAGGGGGGCAGCCC.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTTACCGAAATTGCa      <  1:2285623/101‑1 (MQ=25)
                                                                                             ggggTGAA.TGGC..GAAGCCAG...AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGCaa     >  1:3179071/1‑101 (MQ=25)
                                                                                              gggTGAA.TGGC..GAAGCCAG...AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGCAAc    >  1:1611537/1‑101 (MQ=25)
                                                                                                     |                                                                                                       
GGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAG..AAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAG  >  NC_014034/2493956‑2494151

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG