Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,080 +C 87.9% intergenic (+128/‑114) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0801.→C87.9% 37.0 ‑1.0 ‑0.1107intergenic (+128/‑114)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (56/38):  ref base (6/7):  total (62/45)
Fisher's exact test strand distribution p-value = 3.84e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.42e-01

Only 100 of 126 total aligned reads displayed.

GACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAG..AAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCA  >  NC_014034/2493982‑2494170
                                                                                                          |                                                                                           
 aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.ccc                                                                                          >  2:181846/1‑101 (MQ=17)
  cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.cccc                                                                                         <  1:2553565/101‑1 (MQ=17)
  cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.cccc                                                                                         <  2:1471921/101‑1 (MQ=17)
  cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.cccc                                                                                         >  2:231394/1‑101 (MQ=18)
   tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.ccccc                                                                                        >  2:321368/1‑101 (MQ=18)
   tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.ccccc                                                                                        >  2:633975/1‑101 (MQ=17)
     cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.cccccct                                                                                      >  2:3155734/1‑99 (MQ=17)
     cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.cccccct                                                                                      <  2:640139/101‑3 (MQ=17)
     cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.cccccct                                                                                      <  1:4113550/101‑3 (MQ=17)
     cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.cccccct                                                                                      <  2:936538/101‑3 (MQ=17)
       cccGGGATAGTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCG.cccccctgc                                                                                    <  2:2113354/101‑5 (MQ=17)
         cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGA..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGccc                                                                                   <  1:3748899/101‑1 (MQ=17)
          gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGcccc                                                                                  <  2:3008822/101‑1 (MQ=17)
           ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCcg                                                                                 <  2:1524554/101‑1 (MQ=17)
            gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgc                                                                                >  1:1383546/1‑100 (MQ=0)
            gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgc                                                                                <  1:76569/101‑2 (MQ=0)
            gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCcgg                                                                                >  2:3035939/1‑101 (MQ=18)
             atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgcc                                                                               >  1:1063102/1‑99 (MQ=2)
             atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgcc                                                                               <  1:2038261/101‑3 (MQ=2)
             atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCcggc                                                                               >  2:2615727/1‑101 (MQ=18)
             atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCA..GAGGGGGGC.CGCGCCCCCCCGCCCcggc                                                                               >  1:1983187/1‑101 (MQ=14)
               atTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGC..C.AA.ACGCCAAGAGGGGGAAGCG.CCCCCTGCCCcgccgg                                                                             >  1:3675887/1‑101 (MQ=2)
               atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCcggcgg                                                                             >  1:3984679/1‑101 (MQ=17)
               atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCcggcgg                                                                             >  2:3916301/1‑101 (MQ=18)
                 ttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGCAGCG.CCCCCTGCCCcgccggat                                                                           <  2:3744366/101‑3 (MQ=2)
                  tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTcc                                                                          >  1:3235319/1‑101 (MQ=17)
                   gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCATCCC.CCCCCTGCCCCGGCGGTCCg                                                                         <  2:3138149/101‑1 (MQ=18)
                   gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCg                                                                         <  1:3695775/101‑1 (MQ=17)
                     agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTg                                                                       <  2:1717607/101‑1 (MQ=17)
                     agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATAGCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTg                                                                       <  1:181884/101‑1 (MQ=17)
                       caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGGCCGTggg                                                                     >  1:143678/1‑100 (MQ=17)
                         aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcgg                                                                   >  1:4224779/1‑101 (MQ=17)
                         aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcgg                                                                   >  2:625069/1‑101 (MQ=18)
                           aTGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCAGCTC.CCCCCTGCCCCGGCGGTCCGTGgcggcg                                                                 >  1:2845295/1‑101 (MQ=18)
                           aTGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGgcggcg                                                                 <  1:3195103/101‑1 (MQ=17)
                             gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TAGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGcc                                                               >  1:335947/1‑101 (MQ=17)
                              aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGGGCCt                                                              >  2:911065/1‑101 (MQ=17)
                                aCCGGATGCCTTCCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCAGGCCGTGGGGGGGCCTTg                                                            >  1:932663/1‑101 (MQ=9)
                                 ccGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGa                                                           >  1:2841566/1‑101 (MQ=17)
                                  cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                                          <  1:882183/101‑1 (MQ=17)
                                  cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCA..GAGGGGGGCAGGGC.CCCCCTGCCCCGGCGGGCCGCGGCGGCGCCCTGAc                                                          >  1:3622924/1‑101 (MQ=17)
                                    gATGTCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCG..GAGGGGGGCAGGGC.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc.gg                                                       <  2:1353657/101‑1 (MQ=18)
                                    gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc.gg                                                       <  2:2298375/101‑1 (MQ=17)
                                      tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc.gggc                                                     >  2:517161/1‑101 (MQ=17)
                                       gCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc.gggcg                                                    <  2:2046365/101‑1 (MQ=17)
                                        ccTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc.gggcgg                                                   <  1:1165216/101‑1 (MQ=17)
                                         cTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc.gggcggg                                                  >  2:3396601/1‑101 (MQ=18)
                                          tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCgggg                                                 >  2:3468148/1‑101 (MQ=18)
                                           ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGCGGCGGCGCCTTGAC.GGGCggggc                                                >  2:3271940/1‑100 (MQ=17)
                                            tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGgc                                               >  2:1293961/1‑101 (MQ=18)
                                            tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGgc                                               >  2:1319747/1‑101 (MQ=18)
                                               tCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGGC.GGGCGGGGGcgcg                                            >  2:1136849/1‑101 (MQ=18)
                                                cTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCgg                                           >  2:2713873/1‑101 (MQ=18)
                                                 ttGCCAGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGGCTTGAC.GGGCGGGGGGGCggg                                          >  1:43816/1‑101 (MQ=17)
                                                  tGCCCGAAATATGCGGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGc                                         <  2:2602539/101‑1 (MQ=18)
                                                  tGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGc                                         <  1:734103/101‑1 (MQ=18)
                                                     ccGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCAtt                                      <  1:321413/101‑1 (MQ=18)
                                                      cGAAATTTCCCGGGGGGGTAA.TGGC..G.AA.GCC..AGGGGGGGGCAGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCACTa                                     <  1:2414389/101‑1 (MQ=16)
                                                      cGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTa                                     <  2:2982465/101‑1 (MQ=18)
                                                       gAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTaa                                    <  1:2176365/101‑1 (MQ=18)
                                                       gAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTaa                                    <  2:3159051/101‑1 (MQ=18)
                                                        aagtgtCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAAc                                   <  1:3190945/96‑1 (MQ=18)
                                                           tatCCGGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGa                                <  2:1519688/101‑1 (MQ=21)
                                                           tatCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGa                                >  1:3055170/1‑101 (MQ=21)
                                                           tatCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGa                                >  2:1783617/1‑101 (MQ=21)
                                                            atCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGG.GAGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTCGAC.GGGGGGGGGCGCGGGCAGTAACCCaa                               >  1:640583/1‑101 (MQ=12)
                                                             tCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGaaa                              >  2:2713553/1‑101 (MQ=21)
                                                             tCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGaaa                              >  1:1570566/1‑101 (MQ=21)
                                                              cccGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAAt                             <  2:2775235/101‑1 (MQ=21)
                                                                cGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTg                           >  2:1127724/1‑101 (MQ=25)
                                                                 ggggggTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGc                          <  1:4170531/101‑1 (MQ=25)
                                                                 ggggggTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGc                          >  1:1912598/1‑101 (MQ=25)
                                                                 ggggggTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGAGGTCCGAGGGGGGGCCTTGACTGGGCGGGGG.GCGGGCGTTAACGGaagtggc                          >  1:1608576/1‑96 (MQ=2)
                                                                 ggggggCGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCCTTAACCGAAATTGc                          >  2:1282704/1‑101 (MQ=21)
                                                                  gggggTGAA.AGGC..GAAG.CCA..GAGGGGGGCAGCCC.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTTACCGAAATTGCa                         <  1:2285623/101‑1 (MQ=25)
                                                                   ggggTGAA.TGGC..GAAGCCAG...AGGGGGTC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGCaa                        >  1:2414711/1‑101 (MQ=25)
                                                                   ggggTGAA.TGGC..GAAG.CCA..GAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGCaa                        >  2:832539/1‑101 (MQ=25)
                                                                    gggTGAA.TGGC..GAAGCCAG...AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGCAAc                       >  1:1611537/1‑101 (MQ=25)
                                                                      gTGAA.TGGC..GAAGCCAG...AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAg                     >  1:592488/1‑101 (MQ=25)
                                                                                   gcgaagccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGAtgctgc              <  1:1782045/91‑1 (MQ=38)
                                                                                    cgaagccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGGGCGGGCATTAACCGAAATTGCAACAGATGCTGCg             <  1:4234201/92‑1 (MQ=37)
                                                                                    cgaagccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCg             <  2:1615623/92‑1 (MQ=38)
                                                                                    cgaagccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCg             >  2:4149892/10‑101 (MQ=38)
                                                                                      aagccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGGATTAACCGAAATTGCAACAGATGCTGCGAt           >  2:2928861/8‑101 (MQ=38)
                                                                                        gccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATcc         <  1:883036/96‑1 (MQ=38)
                                                                                         ccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCt        >  1:3176985/5‑101 (MQ=38)
                                                                                         ccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCt        >  1:2936686/5‑101 (MQ=38)
                                                                                         ccagaGGGGGGC.AGCGCCCCCCTGCCCCCGCGGTCCGTGGCGGCGCCTTGAC.GGACGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGTGATCCt        >  1:3514991/5‑101 (MQ=37)
                                                                                          cagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCTAAATTGCAACAGATGCTGCGATCCTc       <  2:3490273/98‑1 (MQ=38)
                                                                                           agaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTTCTTGGGGGCGGCTTACA.GGGAGGGGGCGCCGGCTTTTACCGACATTTCAACCGCCGCTGCTATccacc      >  2:3599750/3‑98 (MQ=2)
                                                                                           agaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCg      >  1:1928907/3‑101 (MQ=39)
                                                                                           agaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCg      >  1:2504183/3‑101 (MQ=39)
                                                                                           agaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCg      >  2:1130566/3‑101 (MQ=39)
                                                                                           agaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCg      >  1:1177481/3‑101 (MQ=39)
                                                                                            gaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGc     >  2:1431756/2‑101 (MQ=39)
                                                                                             aGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGcc    >  2:1646855/1‑101 (MQ=255)
                                                                                             aGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGcc    >  2:1168432/1‑101 (MQ=255)
                                                                                               gggggCAGGCC.CCCCCTTCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGGGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCa  <  1:1990499/101‑1 (MQ=255)
                                                                                               gggggC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCa  >  1:261335/1‑101 (MQ=255)
                                                                                               gggggC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCa  <  2:1814093/101‑1 (MQ=255)
                                                                                                          |                                                                                           
GACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAG..AAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC.GGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCA  >  NC_014034/2493982‑2494170

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 22 ≤  ATCG  < 31 ≤  ATCG  < 39 ≤  ATCG