Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,590,199	+C	100%	intergenic (‑98/‑158)	glnE ← / → RCAP_rcc02415	glutamate‑ammonia‑ligase adenylyltransferase/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,590,199	1	.→C	100.0%	229.4	62	intergenic (‑98/‑158)	glnE/RCAP_rcc02415	glutamate‑ammonia‑ligase adenylyltransferase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (62/0):  ref base (0/0):  total (62/0)

CATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCG.CCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTCACATCCCCTCTTGACCCG  >  NC_014034/2590099‑2590294                                                                                                      |                                                                                                    cATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAgcg                                                                                                      >  1:374662/1‑101 (MQ=255) cATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAgcg                                                                                                      <  2:1893748/101‑1 (MQ=255) cATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAgcg                                                                                                      >  1:2738981/1‑101 (MQ=255)   ggccgcCAGCCTGGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCG.cc                                                                                                   <  2:3995422/98‑1 (MQ=255)      cgcCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCG.ccc..cc                                                                                              >  2:2216463/1‑101 (MQ=255)       gcCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCG.ccc..ccc                                                                                             <  1:2189388/101‑2 (MQ=255)        ccAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCG.ccc..cccg                                                                                            >  2:2818596/1‑99 (MQ=255)        ccAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCG.ccc..cccg                                                                                            <  1:716963/101‑3 (MQ=255)         cAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCG.ccc..cccgc                                                                                           >  1:1100577/1‑98 (MQ=255)          aGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCG.ccc..cccgcc                                                                                          >  1:92352/1‑97 (MQ=255)          aGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCG.ccc..cccgcc                                                                                          >  1:2915689/1‑97 (MQ=255)            aCTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCgg                                                                                       >  1:415760/1‑101 (MQ=255)              tAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCgggc                                                                                     >  1:2765393/1‑100 (MQ=255)              tAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGt                                                                                     >  1:1340096/1‑101 (MQ=255)               aGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTc                                                                                    >  2:1964188/1‑101 (MQ=255)                   gccgcAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCt                                                                                >  1:3272568/1‑101 (MQ=255)                   gccgcAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCt                                                                                >  2:3621088/1‑101 (MQ=255)                   gccgcAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCt                                                                                >  1:927542/1‑101 (MQ=255)                      gcAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCgg                                                                             >  1:452522/1‑101 (MQ=255)                        aaGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGAAGCGCCCC..CC..GCCGGGTCGCCTCGgcg                                                                           >  1:2611769/1‑101 (MQ=255)                             ccGGGTCAAGGCGCGCAACTGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGGGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGc                                                                      >  1:3105792/1‑101 (MQ=38)                             ccGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGc                                                                      >  1:3741818/1‑101 (MQ=255)                                ggTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGGCTCGGCGCAAGCCGa                                                                   >  2:2177538/1‑101 (MQ=255)                                 gTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAg                                                                  >  1:1715274/1‑101 (MQ=255)                                  tCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAgg                                                                 >  2:3503463/1‑101 (MQ=255)                                  tCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAgg                                                                 >  1:4066115/1‑101 (MQ=255)                                    aaGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCg                                                               >  2:724785/1‑101 (MQ=255)                                     aGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGa                                                              >  2:3847785/1‑101 (MQ=255)                                     aGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGa                                                              >  1:699063/1‑101 (MQ=255)                                     aGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGCCGCCACCGGGCAAGAAGAGGAGa                                                              >  2:2474728/1‑101 (MQ=25)                                        cgcgcAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAAt                                                           >  2:1353253/1‑101 (MQ=255)                                            cAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAAtctct                                                       >  2:2622711/1‑101 (MQ=255)                                             aaaTGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAAtctctc                                                      >  1:1080145/1‑101 (MQ=255)                                               aTGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCCAGCCGAGGCGGAATCTCTccc                                                    >  2:793232/1‑101 (MQ=38)                                               aTGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTccc                                                    >  1:2147902/1‑101 (MQ=255)                                                tGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCCGCGCCCC..CC..GCCGGGACGCCTAGGCGCAAGCCCAGGCGAAATCTCTcccc                                                   >  2:725406/1‑101 (MQ=37)                                                 gccccgccccTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTccccc                                                  >  1:926691/1‑100 (MQ=255)                                                 gccccgccccTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCg                                                  >  2:465470/1‑101 (MQ=255)                                                  ccccgccccTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGa                                                 >  2:3211642/1‑101 (MQ=255)                                                    ccgccccTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGAcc                                               >  1:1514830/1‑101 (MQ=255)                                                      gccccTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGAcccc                                             >  1:700367/1‑101 (MQ=255)                                                        cccTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCtt                                           >  2:3164000/1‑101 (MQ=255)                                                        cccTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCtt                                           >  2:3303971/1‑101 (MQ=255)                                                         ccTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTg                                          >  2:1213809/1‑101 (MQ=255)                                                          cTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTgc                                         >  1:2137051/1‑101 (MQ=255)                                                            gTCATCTTGGCGCGAATATCCCGGGGGGGGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTgcgg                                       >  2:1357813/1‑101 (MQ=255)                                                              cATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTgcggcg                                     >  1:3510535/1‑101 (MQ=255)                                                              cATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTgcggcg                                     >  2:3464432/1‑101 (MQ=255)                                                              cATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGCCGCCTCGGCGCAAGCCGTGGCGAAATGTCTCACCGGCCCCttcacgcc                                     >  1:10650/1‑95 (MQ=21)                                                                  ttGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAAt                                 >  2:3535822/1‑101 (MQ=255)                                                                        cgAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTaaaa                           >  2:3781594/1‑101 (MQ=255)                                                                        cgAATATCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGACGCCGCGGCGCAAGCCGAGGCGCAATCTCTCCCCGACCCCTAGCGGCGCCCTGAaaaa                           >  1:717355/1‑101 (MQ=25)                                                                          aagccccgggggggGGCGGGGGGCCCCC.CCC..CCCGGCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTaaaaa                          <  2:2575968/93‑1 (MQ=25)                                                                            tttCCCGGGGGGGCGGGGGGGCCCCC.CCC..CCCGGCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATc                        <  1:2818815/99‑1 (MQ=25)                                                                            tatCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCt                       >  1:3436878/1‑101 (MQ=255)                                                                             atCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCtt                      >  1:722210/1‑101 (MQ=255)                                                                             atCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCtt                      >  2:205898/1‑101 (MQ=255)                                                                             atCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCtt                      >  2:3997117/1‑101 (MQ=255)                                                                             atCCCGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCtt                      >  1:3870142/1‑101 (MQ=255)                                                                                 gggggggGGGGGGGGGCCGCG.CCCCGCCG.GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTc                    <  1:3349871/100‑1 (MQ=25)                                                                                 cGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGCGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATATTTcac                  >  1:1534146/1‑101 (MQ=38)                                                                                 cGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTcac                  >  2:2034652/1‑101 (MQ=255)                                                                                 cGGGGGGTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTcac                  >  2:142636/1‑101 (MQ=255)                                                                                  ggggggTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTcaca                 >  2:346939/1‑101 (MQ=255)                                                                                  ggggggTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTcaca                 >  2:2754453/1‑101 (MQ=255)                                                                                   gggggTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGGAAAAATCTTTCACAt                >  2:157779/1‑101 (MQ=255)                                                                                      ggTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCCAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCCGCGCAATGTAAAAATCTTTCACATccc             >  2:3649471/1‑101 (MQ=38)                                                                                      ggTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTCACATccc             >  1:2142609/1‑101 (MQ=255)                                                                                      ggTGCGGGGGGCAGCGCCCC..CC..GCCGGGGCGCCCCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCCTGCGGCGCAATGTAAAAATCTTTCACATccc             >  1:2867137/1‑101 (MQ=255)                                                                                       gTGCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTCACATcccc            >  2:3093050/1‑101 (MQ=255)                                                                                         gCGGGGGGCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTCACATCCCctc          >  1:3210325/1‑101 (MQ=255)                                                                                           ggggggCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAGATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTCACATCCCCTCtt        >  2:3659416/1‑101 (MQ=255)                                                                                           ggggggCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTCACATCCCCTCtt        >  2:3502943/1‑101 (MQ=255)                                                                                           ggggggCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTCACATCCCCTCtt        >  1:2070846/1‑101 (MQ=255)                                                                                           ggggggCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTCACATCCCCTCtt        >  1:649984/1‑101 (MQ=255)                                                                                           ggggggCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTCACATCCCCTCtt        >  1:1411565/1‑101 (MQ=255)                                                                                             ggggCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTCACATCCCCTCTTga      >  1:2053179/1‑101 (MQ=255)                                                                                               ggCAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTCACATCCCCTCTTgacc    >  2:1951901/1‑101 (MQ=255)                                                                                                 cAGCGCCCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTCACATCCCCTCTTgacccg  >  2:796190/1‑101 (MQ=255)                                                                                                      |                                                                                                    CATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGGGGGGCAGCG.CCC..CC..GCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTCACATCCCCTCTTGACCCG  >  NC_014034/2590099‑2590294 Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG