Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,678,900 +C 96.0% intergenic (+29/+58) RCAP_rcc02495 → / ← RCAP_rcc02496 GTPase, EngC family/group 1 glycosyl transferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,678,9001.→C96.0% 8.8 ‑4.3 ‑0.1125intergenic (+29/+58)RCAP_rcc02495/RCAP_rcc02496GTPase, EngC family/group 1 glycosyl transferase
Reads supporting (aligned to +/- strand):  new base (71/49):  ref base (0/5):  total (71/54)
Fisher's exact test strand distribution p-value = 1.35e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.45e-01

Only 100 of 162 total aligned reads displayed.

GCCCGCGACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CG.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCGACAAAGCG  >  NC_014034/2678800‑2678993
                                                                                                       |                                                                                             
gCCCGCGACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.Cg                                                                                                >  1:3817117/1‑101 (MQ=255)
    gcgACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CG.cccc                                                                                           >  1:2206010/1‑101 (MQ=255)
     cgACCGCGCCTTCGGCAAGATGGCCCGGGCGCCGCTGCGCGACAAATCCCGGCTGCGCGGCTACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CG.ccccc                                                                                          <  1:3501980/101‑2 (MQ=255)
     cgACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CG.ccccc                                                                                          >  1:2177018/1‑100 (MQ=255)
     cgACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CG.ccccc                                                                                          <  1:1941061/101‑2 (MQ=255)
      gACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CG.ccccca                                                                                         >  2:126756/1‑99 (MQ=255)
      gACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CG.ccccca                                                                                         >  2:1955933/1‑99 (MQ=255)
      gACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CG.ccccca                                                                                         <  2:3402597/101‑3 (MQ=255)
       aCCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CG.cccccac                                                                                        <  1:140968/101‑4 (MQ=255)
        ggatttcTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CG.cccccacc                                                                                       >  2:3334305/7‑97 (MQ=255)
         cgcgCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CG.cccccaccg                                                                                      >  2:2171675/1‑96 (MQ=255)
         cgcgCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CG.cccccaccg                                                                                      >  2:3458343/1‑96 (MQ=255)
           cgcCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACcgcg                                                                                     >  1:2740035/1‑101 (MQ=255)
           cgcCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACcgcg                                                                                     <  1:1699306/101‑1 (MQ=255)
            gcCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCgcgc                                                                                    >  1:107019/1‑101 (MQ=255)
            gcCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCgcgc                                                                                    >  2:2521147/1‑101 (MQ=255)
            gcCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCgcgc                                                                                    >  1:1506810/1‑101 (MQ=255)
             ccTTCGACAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGcc                                                                                   <  2:2071504/101‑1 (MQ=255)
              cTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGccc                                                                                  >  1:14235/1‑101 (MQ=255)
               ttCGGCAAGATGGCCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCg                                                                                 <  2:2043663/101‑1 (MQ=255)
                 cGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCgcc                                                                               >  2:2993308/1‑99 (MQ=255)
                  ggcagagggACCCGGGCGGCGCAGCGATACAAATCCCGGCGGCGCTGCGACTCACGCCGGTTCGGGGGCCGGTGGGG.ACAT.CGCCCCCACCGCGCCCGAcg                                                                              <  2:1660744/93‑1 (MQ=255)
                    cAAGAAGCCCCGGGGGGCGCTGCGCGCCAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCCCCGCGCCCGAcgcg                                                                            <  2:4179253/101‑1 (MQ=255)
                     aaGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGAcgcgc                                                                           >  2:3714590/1‑101 (MQ=255)
                      aGATGCCCCGGGCGGCGCTGCGCGACAAACCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGAcgcgcg                                                                          <  1:2310926/101‑1 (MQ=255)
                         tgccccGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCgcggcg                                                                       <  1:1375592/98‑1 (MQ=255)
                          gACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCCCCGCGCCCGACGCGcggcgg                                                                      >  2:3063097/1‑101 (MQ=255)
                          gACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGcggcgg                                                                      <  2:3498341/101‑1 (MQ=255)
                           gtccGGGGGGTGCTTCGCAACAAATCCCGGCTGCGCCGCGACTGGCGCCGGTTGGGGTGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCggg                                                                     <  2:2733318/99‑1 (MQ=25)
                              cGGGGGGCGCTGCGCGACAAATCCAGGCTGCGCGGCGACTGACGCCGGTTCGGGGGGCGGGGGTG.GCCT.CGCCCCCCCCGCGCCCAACGCCCCGCCGGCtt                                                                  >  2:1838556/1‑101 (MQ=255)
                              cGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCtt                                                                  >  1:1290844/1‑101 (MQ=255)
                               gggCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTg                                                                 >  2:1565148/1‑101 (MQ=255)
                                ggcggcGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTgc                                                                <  2:1479613/101‑1 (MQ=255)
                                  gggcgCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTgccc                                                              <  1:2521331/100‑1 (MQ=255)
                                   ggcgCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTgcccg                                                             >  1:1469429/1‑101 (MQ=255)
                                      gcggcgcGACAACCCCGGGCGGCGCGGCGACTGGCGCGGGTGGGGGGGCGGGGGGGGCCCCC.CC.CCCCCCCGCGCCCGACGCGCGGCGGGCTTgcccgccc                                                          <  1:2993446/98‑1 (MQ=12)
                                      gcTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTgcccgccc                                                          >  2:3466703/1‑101 (MQ=255)
                                      gcTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTgcccgccc                                                          <  2:518863/101‑1 (MQ=255)
                                       cTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCa                                                         >  2:2570946/1‑101 (MQ=255)
                                        tGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTgcccgcccca                                                        >  2:1794039/1‑99 (MQ=255)
                                        tGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCaa                                                        >  1:1374450/1‑101 (MQ=255)
                                         gcgcGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAag                                                       >  1:3708030/1‑101 (MQ=255)
                                           gcgCCAAACCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagga                                                     <  1:1247469/101‑1 (MQ=37)
                                             gACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAaggagg                                                   <  2:1740387/101‑1 (MQ=255)
                                             gACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAaggagg                                                   >  1:464351/1‑101 (MQ=255)
                                             gACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAaggagg                                                   >  2:3221499/1‑101 (MQ=255)
                                             gACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAaggagg                                                   >  2:1360213/1‑101 (MQ=255)
                                             gACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCCTGCCCGCCCCaggagg                                                   >  2:309796/1‑101 (MQ=37)
                                               cAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCCCCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGgc                                                 <  1:4147213/101‑1 (MQ=255)
                                                  aTCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGcc                                              >  2:784498/1‑101 (MQ=255)
                                                  aTCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGcc                                              <  1:620181/101‑1 (MQ=255)
                                                   tCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGccc                                             >  2:1181002/1‑101 (MQ=255)
                                                    cccGGCTGCGCGGCGACTGACGCCTGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGAGCGGCGGGCTTGCCCGCCCAAGGAGGGCGcccc                                            >  1:3607780/1‑101 (MQ=25)
                                                    cccGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGcccc                                            >  1:4038075/1‑101 (MQ=255)
                                                     ccGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGccccc                                           <  1:2490165/101‑1 (MQ=255)
                                                       ggCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCgg                                         >  1:846669/1‑101 (MQ=255)
                                                       ggCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCgg                                         >  1:2765129/1‑101 (MQ=255)
                                                       ggCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCgg                                         <  1:2387100/101‑1 (MQ=255)
                                                       ggCGGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCgg                                         <  1:126780/101‑1 (MQ=255)
                                                           gagcgggtgcTGCCGGCGGTTGGGGGGCCGGTGGGG.GCAT.CGCCCCCGCGGCGCCCGACGCGCGGGGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGAtct                                     <  2:2320246/92‑1 (MQ=17)
                                                            cgcgGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGGGGGGCCCCCCGGAtccc                                    >  1:2378808/1‑99 (MQ=25)
                                                            cgcgGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGAtctc                                    <  1:1549187/101‑1 (MQ=255)
                                                            cgcgGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGAtctc                                    >  2:3188126/1‑101 (MQ=255)
                                                             gcggcgACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCa                                   <  2:2178360/101‑1 (MQ=255)
                                                             gcggcgACTGACGCCGGTTCGGGGGCCGGGGGGG.CCAT.CGCCCCCCCCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCa                                   <  1:198902/101‑1 (MQ=25)
                                                              gggcgACTGACGCCGGTCCGGGGCCCGGTGGGT.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAg                                  <  2:4115908/100‑1 (MQ=25)
                                                               ggcgACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAgc                                 >  2:766955/1‑101 (MQ=255)
                                                                  gACTGACGCGGGTTCGGGGGCGGGTGGGA.GCATCCC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCa                              <  2:3522335/101‑1 (MQ=25)
                                                                  gACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCa                              >  1:2856606/1‑101 (MQ=255)
                                                                  gACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCa                              >  2:933859/1‑101 (MQ=255)
                                                                    cTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAAc                            <  2:2861497/101‑1 (MQ=255)
                                                                     tGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACg                           >  2:2032851/1‑101 (MQ=255)
                                                                       gcGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTg                         <  2:2956967/100‑1 (MQ=255)
                                                                       aCGCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTg                         <  2:375207/101‑1 (MQ=255)
                                                                        cGCCGGTTCGGGGGCCGGTGGGG.GCATCCC.CCCCCCCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTgc                        <  1:1181096/101‑1 (MQ=25)
                                                                         gCCGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTgcc                       <  2:3182460/101‑1 (MQ=255)
                                                                          ccGGTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTgccg                      <  1:3425720/101‑1 (MQ=255)
                                                                             ttcggggggCCGGTGGGG.GCTTCCC.CCCCACCGCCCCCGACGCGCGGCGGGCTTTCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGccgccc                   <  1:3460188/97‑1 (MQ=25)
                                                                             gTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGccgccc                   >  2:3677740/1‑101 (MQ=255)
                                                                             gTTCGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGccgccc                   >  1:544244/1‑101 (MQ=255)
                                                                              tgcGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGccgcccg                  <  2:1466098/99‑1 (MQ=255)
                                                                                cGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCcgcccgcc                >  2:2687653/1‑101 (MQ=255)
                                                                                cGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCcgcccgcc                >  1:1884927/1‑101 (MQ=255)
                                                                                cGGGGGCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCcgcccgcc                >  2:1196618/1‑101 (MQ=255)
                                                                                cGGGGGCCGGGGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCcgcccgcc                <  1:286556/101‑1 (MQ=255)
                                                                                 gggggCGGGTGGGG.GCAC.CGCCCCCCCCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCccgccg               <  1:1956059/101‑1 (MQ=21)
                                                                                 gggggCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCACGCCCAAGGAGGGCGCCCCCGGAGCTCAGCGCAACGCgccgccgaacg               >  1:3503753/1‑96 (MQ=21)
                                                                                    ggCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGtct            >  2:3142579/1‑101 (MQ=255)
                                                                                    ggCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGtct            >  1:3084862/1‑101 (MQ=255)
                                                                                    ggCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGtct            >  1:3122539/1‑101 (MQ=255)
                                                                                     gCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGtctc           <  2:1926575/101‑1 (MQ=255)
                                                                                     gCCGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGtcta           >  2:3371816/1‑100 (MQ=255)
                                                                                       cGGTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCGa         >  1:740228/1‑101 (MQ=255)
                                                                                       cGGTGGGG.CCCT.CGCCCCCGCCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCGa         <  1:1851376/101‑1 (MQ=25)
                                                                                        ggggggg.gCAC.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCGAc        <  1:3334550/98‑1 (MQ=25)
                                                                                        ggTGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCGAc        >  1:331757/1‑101 (MQ=255)
                                                                                          tGGGG.GCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCGACaa      >  2:1600134/1‑101 (MQ=255)
                                                                                           gggg.gCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGGCTCGCCaca     >  1:768923/1‑99 (MQ=25)
                                                                                           gggg.gCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCG.TCGCCGTCTCgtattcg    <  2:14224/101‑7 (MQ=21)
                                                                                              g.gCAT.CGCCCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCGACAAAGCg  >  1:3685151/1‑101 (MQ=255)
                                                                                                       |                                                                                             
GCCCGCGACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCAT.CG.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCGACAAAGCG  >  NC_014034/2678800‑2678993

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG