Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,747,849	+G	100%	intergenic (‑181/+112)	RCAP_rcc02553 ← / ← RCAP_rcc02554	hypothetical protein/NmrA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,747,849	1	.→G	100.0%	63.6	22	intergenic (‑181/+112)	RCAP_rcc02553/RCAP_rcc02554	hypothetical protein/NmrA family protein
Reads supporting (aligned to +/- strand):  new base (5/17):  ref base (0/0):  total (5/17)

TCGGGCGGAATAGGAAACGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.C.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGG  >  NC_014034/2747751‑2747943                                                                                                     |                                                                                                gggggCGAAATAGGAACCGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggcc.c.gg                                                                                               <  2:2228632/99‑2 (MQ=255)   gggCGGAATAGGAAACGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggcc.c.ggcg                                                                                             >  1:3484999/1‑98 (MQ=255)   gggCGGAATAGGAAACGCGACCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggcc.c.ggcg                                                                                             <  2:2341297/101‑4 (MQ=255)     gggaaaTAGGAAACGGGCCCCCCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggcc.c.ggcggg                                                                                           <  2:247893/97‑6 (MQ=37)     gCGGAATAGGAAACGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggcc.c.ggcggg                                                                                           <  1:1413533/101‑6 (MQ=255)       ggAATGGGAACCCCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggcc.c.ggcgggcg                                                                                         <  2:399102/101‑8 (MQ=37)        gAATAGGAAACGCGCCCCGGGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggcc.c.ggcgggcga                                                                                        <  2:1119178/101‑9 (MQ=37)        gAACAGAAAGCGCGCCCCCCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggcc.c.ggcgggcga                                                                                        <  2:1465338/101‑9 (MQ=25)          atgggAAACTCGCCCGACGTTTCCCCGCCCGCAGCTCACGGTTGCGGCATGACCCCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCgaga                                                                                       <  2:1698458/98‑1 (MQ=21)          agagaaaccgcgCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCgaga                                                                                       <  1:898396/93‑1 (MQ=25)           tcggAAACGCGGCCCGCTTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGaa                                                                                      <  2:1362957/99‑1 (MQ=25)            aGGAACCGCGCCCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAg                                                                                     <  2:3426537/101‑1 (MQ=37)            aGGAAACGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAg                                                                                     >  2:889843/1‑101 (MQ=255)               aaccgcgCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAgcgc                                                                                  <  1:1448915/98‑1 (MQ=25)               aaccgcgCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAgcgc                                                                                  <  2:3879171/98‑1 (MQ=25)                 ccgcgCCCCGCGGTCCCCCGCCCGCAGCGCCCGGTTGCGGCCTGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGcgccc                                                                                <  1:3099668/100‑1 (MQ=21)                   gcgCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGcgcccgc                                                                              <  1:1706810/101‑1 (MQ=25)                    cccccctgctttCCCCGCCCGCAGCGTCCGGTTGCGGCATGACCGCTTCAGCCCGCCCCCCAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGcgcccgcc                                                                             <  2:242858/92‑1 (MQ=18)                     cccccGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCccgcc.g                                                                           <  2:4106001/99‑1 (MQ=25)                      ccccggtttcccccGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCATCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gg                                                                          <  2:2113541/91‑1 (MQ=21)                      ccccGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gg                                                                          <  2:3809635/100‑1 (MQ=25)                       cccGCTTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.ggg                                                                         <  2:2420095/101‑2 (MQ=25)                        ccGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCGgcc.gggc                                                                        <  1:226249/101‑3 (MQ=25)                        ccGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gggc                                                                        <  2:1972616/101‑3 (MQ=25)                         cggtttcccccGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gggcc                                                                       <  2:3534774/94‑4 (MQ=21)                           cgTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCccc.gggccgg                                                                     >  1:3164991/1‑96 (MQ=25)                             tccccccGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gggccgggc                                                                   <  2:2514919/98‑8 (MQ=21)                              ttCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gggccgggcg                                                                  >  1:3770226/1‑93 (MQ=25)                                                      cgccaggcccGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGCCCGG.GCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTc                                           <  2:3769969/93‑1 (MQ=17)                                                                           caccacAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCtgtg                      >  2:3225047/1‑101 (MQ=14)                                                                           caccacAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGGCACAGGACCTCtgtg                      >  2:2589593/1‑101 (MQ=14)                                                                                           gcccggcc.cgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCg      >  1:2680314/1‑101 (MQ=18)                                                                                             ccggcc.cgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGAt    >  1:991505/1‑101 (MQ=18)                                                                                              cggcc.cgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATg   >  1:2270128/1‑101 (MQ=18)                                                                                               ggcc.cgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATgg  >  2:177914/1‑101 (MQ=18)                                                                                               ggcc.cgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATgg  >  1:349115/1‑101 (MQ=18)                                                                                                     |                                                                                                TCGGGCGGAATAGGAAACGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.C.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGG  >  NC_014034/2747751‑2747943 Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG