Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,747,869	+G	100%	intergenic (‑201/+92)	RCAP_rcc02553 ← / ← RCAP_rcc02554	hypothetical protein/NmrA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,747,869	1	.→G	100.0%	48.7	19	intergenic (‑201/+92)	RCAP_rcc02553/RCAP_rcc02554	hypothetical protein/NmrA family protein
Reads supporting (aligned to +/- strand):  new base (18/1):  ref base (0/0):  total (18/1)

CGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.C.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTCAT  >  NC_014034/2747770‑2747964                                                                                                       |                                                                                                cccccctgctttCCCCGCCCGCAGCGTCCGGTTGCGGCATGACCGCTTCAGCCCGCCCCCCAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGcgcccgcc                                                                                                  <  2:242858/92‑1 (MQ=18)  cccccGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCccgcc.g                                                                                                <  2:4106001/99‑1 (MQ=25)   ccccggtttcccccGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCATCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gg                                                                                               <  2:2113541/91‑1 (MQ=21)   ccccGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gg                                                                                               <  2:3809635/100‑1 (MQ=25)    cccGCTTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.ggg                                                                                              <  2:2420095/101‑2 (MQ=25)     ccGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCGgcc.gggc                                                                                             <  1:226249/101‑3 (MQ=25)     ccGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gggc                                                                                             <  2:1972616/101‑3 (MQ=25)      cggtttcccccGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gggcc                                                                                            <  2:3534774/94‑4 (MQ=21)        cgTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCccc.gggccgg                                                                                          >  1:3164991/1‑96 (MQ=25)          tccccccGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gggccgggc                                                                                        <  2:2514919/98‑8 (MQ=21)           ttCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gggccgggcg                                                                                       >  1:3770226/1‑93 (MQ=25)                                   cgccaggcccGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGCCCGG.GCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTc                                                                <  2:3769969/93‑1 (MQ=17)                                                        caccacAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCtgtg                                           >  2:3225047/1‑101 (MQ=14)                                                        caccacAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGGCACAGGACCTCtgtg                                           >  2:2589593/1‑101 (MQ=14)                                                                        gcccggcc.cgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCg                           >  1:2680314/1‑101 (MQ=18)                                                                          ccggcc.cgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGAt                         >  1:991505/1‑101 (MQ=18)                                                                           cggcc.cgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATg                        >  1:2270128/1‑101 (MQ=18)                                                                            ggcc.cgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATgg                       >  2:177914/1‑101 (MQ=18)                                                                            ggcc.cgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATgg                       >  1:349115/1‑101 (MQ=18)                                                                                 cggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCtt                   >  1:81200/3‑101 (MQ=21)                                                                                    cgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGACCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGACTTGGc                >  2:2129027/1‑101 (MQ=21)                                                                                     gggcGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGcc               >  2:2885984/4‑101 (MQ=21)                                                                                      ggcGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCa              >  1:3278926/3‑101 (MQ=21)                                                                                        cGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAg            >  1:3681922/1‑101 (MQ=21)                                                                                        cGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAg            >  1:3489626/1‑101 (MQ=21)                                                                                         gagaAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAgg           >  2:2985774/1‑101 (MQ=21)                                                                                         gagaAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAgg           >  1:855434/1‑101 (MQ=21)                                                                                         gagaAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAgg           >  1:286273/1‑101 (MQ=21)                                                                                             aGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAg       >  1:728934/1‑101 (MQ=21)                                                                                             aGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGGCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAg       >  1:3818703/1‑101 (MQ=21)                                                                                                gcccgccgGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTc    >  1:4010350/1‑101 (MQ=21)                                                                                                gcccgccgGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTc    >  1:1967331/1‑101 (MQ=21)                                                                                                  ccgccgGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTCAt  >  1:1851148/1‑101 (MQ=21)                                                                                                       |                                                                                                CGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.C.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTCAT  >  NC_014034/2747770‑2747964 Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 39 ≤  ATCG