Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,808,557 +G 100% intergenic (+112/+42) pyrD2 → / ← acsA2 dihydroorotate oxidase/acetate‑‑CoA ligase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,808,5571.→G100.0% 126.9 59intergenic (+112/+42)pyrD2/acsA2dihydroorotate oxidase/acetate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  new base (31/28):  ref base (0/0):  total (31/28)

CTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGA.GTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCGCC  >  NC_014034/2808457‑2808593
                                                                                                     |                                    
cTGCGGGGGTTTCGCACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAATGCACAGACTTTTGCCCGTTCACCCTGGCGCAACCCTCCCGGGTGgaga                                       >  2:2792989/1‑101 (MQ=14)
cTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGgaga                                       >  2:1096458/1‑101 (MQ=38)
cTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGgaga                                       >  1:1378902/1‑101 (MQ=38)
cTGCGGGGCTTTCACACCCCCGCACCCCCGTGGTGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCGCGGGGGgaga                                       <  1:3656586/101‑1 (MQ=255)
 tGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGaga.g                                     <  1:1639972/101‑1 (MQ=37)
  gCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGaga.gg                                    <  1:452311/101‑2 (MQ=21)
  gCGGGGGATTCCCCCCCCCGCAACCCCCGGGCGGAATTGCGCCAAAAAAAAGCCCAGAAATTTGCCCGTTCACCCTGGCGGAAAATTCCCCGGGGGaga.gg                                    >  2:3410005/1‑100 (MQ=2)
     ggggTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGaga.ggtgg                                 >  2:3707424/1‑97 (MQ=17)
     ggggTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGaga.ggtgg                                 <  1:3527186/101‑5 (MQ=14)
      gggTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGaga.ggtggc                                >  2:2359738/1‑96 (MQ=11)
      gggTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGaga.ggtggc                                <  2:801397/101‑6 (MQ=11)
      gggTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGaga.ggtggc                                >  1:2442431/1‑96 (MQ=14)
      gggTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGaga.ggtggc                                >  1:3390093/1‑96 (MQ=11)
        gTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGgcgc                               >  2:293718/1‑101 (MQ=2)
         tttCACACCCCCGCACCCCCGTGGCGTATTTGCGTCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCa                              <  1:3629382/101‑1 (MQ=2)
         tttCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCa                              >  2:3880438/1‑101 (MQ=2)
          ttCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAg                             >  1:211594/1‑101 (MQ=12)
          ttCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAg                             <  2:2824720/101‑1 (MQ=9)
          ttCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAg                             >  2:4126511/1‑101 (MQ=12)
           tCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAgg                            >  1:3975767/1‑101 (MQ=12)
           tCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAgg                            >  1:3904674/1‑101 (MQ=16)
            cacaCCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGc                           >  1:1498737/1‑101 (MQ=12)
            cacaCCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGACGCAAATCTCCCGGGGGGGGAGGTGGCGCAGGc                           >  1:3629342/1‑101 (MQ=11)
             acacCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCa                          >  1:60489/1‑101 (MQ=16)
             acacCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCa                          <  1:585256/101‑1 (MQ=12)
              cccccccgcccccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCCCCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCaa                         <  1:1927310/99‑1 (MQ=11)
              cacccccgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCaa                         <  1:3283927/101‑1 (MQ=12)
              cacccccgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCaa                         <  1:912468/101‑1 (MQ=12)
              cacccccgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCaa                         >  1:4018003/1‑101 (MQ=16)
               ccccccgcaccaccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAg                        <  2:3040989/100‑1 (MQ=255)
               acccccgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAg                        >  2:1956218/1‑101 (MQ=19)
               acccccgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAg                        >  1:31840/1‑101 (MQ=19)
               acccccgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAg                        >  2:3829109/1‑101 (MQ=19)
                cccccgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCGCCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGt                       <  1:3261509/101‑1 (MQ=255)
                cccccgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGt                       <  1:546163/101‑1 (MQ=12)
                 ccccgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTc                      >  1:121646/1‑101 (MQ=12)
                 ccccgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTc                      <  1:962163/101‑1 (MQ=12)
                  cccgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCg                     >  1:1445844/1‑101 (MQ=19)
                    cgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCggg                   >  2:3322456/1‑101 (MQ=19)
                    cgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCggg                   <  1:3896028/101‑1 (MQ=12)
                    cgcaccccagTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCggg                   <  1:502345/101‑1 (MQ=255)
                     gcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCgggg                  >  2:1547128/1‑101 (MQ=19)
                     gcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCgggg                  <  1:400536/101‑1 (MQ=12)
                     gcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCgggg                  <  1:1392686/101‑1 (MQ=12)
                     ccatccccgTAGCGTATTTGGGACAAGAAAACGCACAGACAATGGCCCGTGCACTCTGGCGAAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCgggg                  <  2:1020236/97‑1 (MQ=2)
                      cacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCggggg                 >  1:2227593/1‑101 (MQ=19)
                      cacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCggggg                 >  2:2328466/1‑101 (MQ=19)
                       acccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCgggggg                >  2:2960691/1‑101 (MQ=19)
                        cccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGggc               <  1:3262186/101‑1 (MQ=16)
                        cccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGggc               <  2:1960359/101‑1 (MQ=16)
                        cccccgGGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGggc               <  2:1659100/101‑1 (MQ=255)
                          ttccgatcttATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGggcgg             <  1:1916892/92‑1 (MQ=11)
                          cccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGggcgg             <  2:1293963/101‑1 (MQ=16)
                          cccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGggcgg             <  1:1101968/101‑1 (MQ=16)
                           ccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGggcggc            <  2:623322/101‑1 (MQ=16)
                           ccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGggcggc            >  2:960003/1‑101 (MQ=16)
                           ccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGggcggc            >  1:3241390/1‑101 (MQ=16)
                             gTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGgcgc          >  1:2429122/1‑101 (MQ=16)
                              tGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGcc         >  1:1115083/1‑101 (MQ=16)
                              tGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGcc         >  1:597341/1‑101 (MQ=16)
                              tGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGCGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGcc         >  2:2070201/1‑101 (MQ=255)
                               ggCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGccc        >  2:2931998/1‑101 (MQ=16)
                               ggCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGCCGGCGccc        <  2:121622/101‑1 (MQ=255)
                               ggCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTAGGGGGGCGGCGccc        >  1:469508/1‑101 (MQ=255)
                                gCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGcccc       <  1:680920/101‑1 (MQ=12)
                                 cGTATTTGCGCCAAGAAAAAGCACAGACTTTTTCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGGGGCGCAGGCAAGTCGGGGGGCGGCGccgcc      >  1:210547/1‑101 (MQ=11)
                                 cGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGccccc      <  2:3083067/101‑1 (MQ=12)
                                 cGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGccccc      >  2:3902936/1‑101 (MQ=16)
                                  gTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGcccccc     <  2:1114989/101‑1 (MQ=12)
                                   tATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGggcggagatcggaa    >  2:1916766/1‑92 (MQ=11)
                                   tATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCg    >  2:503083/1‑101 (MQ=12)
                                   tATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCg    <  2:2559934/101‑1 (MQ=12)
                                    aTTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAACTCTCCCGGGGGGAGAGGTGGCGAAGGGCAGGCGGGGGGCGGAGCCCCCCGc   >  2:2011743/1‑101 (MQ=11)
                                    aTTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCGc   <  2:854890/101‑1 (MQ=9)
                                     tttGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCGcc  >  1:701275/1‑101 (MQ=2)
                                     tttGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGGGGCGGAGGCAAGTCGGGGGGGGGGGCCCCCCggc  >  2:1297503/1‑99 (MQ=11)
                                                                                                     |                                    
CTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGA.GTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCGCC  >  NC_014034/2808457‑2808593

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG