Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 296,280 +T 3.8% intergenic (‑80/‑203) RCAP_rcc00258 ← / → RCAP_rcc00259 lipoprotein/porin family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034296,2800.→T 3.8% 20.1 0.0 ‑0.2207intergenic (‑80/‑203)RCAP_rcc00258/RCAP_rcc00259lipoprotein/porin family protein
Reads supporting (aligned to +/- strand):  new base (4/2):  ref base (117/82):  total (121/86)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.20e-01

Only 100 of 241 total aligned reads displayed.

CAAAGAGGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTCCCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTCCCGCCGGTCCCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCGCTTCGG  >  NC_014034/296180‑296380
                                                                                                                                                                                                         
   agagGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcccc                                                                                                   >  2:2658114/1‑101 (MQ=255)
   agagGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcccc                                                                                                   >  2:1486692/1‑101 (MQ=255)
   agagGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcccc                                                                                                   <  1:3217591/101‑1 (MQ=255)
    gagGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTccccc                                                                                                  <  1:412399/101‑1 (MQ=255)
     agGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcccccg                                                                                                 >  2:3858234/1‑100 (MQ=255)
      ggTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcccccgc                                                                                                >  1:1369174/1‑99 (MQ=255)
      ggTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcccccgc                                                                                                >  1:4064054/1‑99 (MQ=255)
       gTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcccccgcg                                                                                               >  1:3163077/1‑98 (MQ=255)
          gCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGa                                                                                           >  1:88778/1‑101 (MQ=255)
          gCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGa                                                                                           <  2:3914956/101‑1 (MQ=255)
            tttCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAcc                                                                                         <  2:3577630/101‑1 (MQ=255)
            tttCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAcc                                                                                         <  2:3031756/101‑1 (MQ=255)
             ttcttcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccg                                                                                        <  2:1369074/101‑1 (MQ=255)
               cttcATCCGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgcc                                                                                      <  2:1972780/101‑1 (MQ=255)
               cttcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgcc                                                                                      >  2:314732/1‑101 (MQ=255)
               cttcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgcc                                                                                      >  1:1276475/1‑101 (MQ=255)
               cttcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgcc                                                                                      >  1:2255205/1‑101 (MQ=255)
               cttcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgcc                                                                                      <  1:2492306/101‑1 (MQ=255)
                  cATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgcccgc                                                                                   >  1:2847178/1‑101 (MQ=255)
                  cATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgcccgc                                                                                   >  1:2780686/1‑101 (MQ=255)
                  cATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgcccgc                                                                                   >  2:1645242/1‑101 (MQ=255)
                     aCGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCgcggc                                                                                >  2:65028/1‑101 (MQ=255)
                         tCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCgggg                                                                            >  2:453031/1‑101 (MQ=255)
                           gACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGCTTTTT.CCCCCGCGGGACCGCCCGCGGCGGGgcg                                                                          >  1:3625285/1‑101 (MQ=255)
                           gACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCgggggg                                                                          >  2:3231678/1‑99 (MQ=255)
                              tGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGTGCGCaa                                                                       >  1:685025/1‑101 (MQ=255)
                               gCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAAc                                                                      >  1:2057985/1‑101 (MQ=255)
                               gCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAAc                                                                      >  2:789388/1‑101 (MQ=255)
                                  cccGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTc                                                                   >  2:2422670/1‑101 (MQ=255)
                                    cGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTccg                                                                 <  2:3343844/101‑2 (MQ=255)
                                      cAGGCTGACCCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTccgcc                                                               <  1:3713515/101‑4 (MQ=255)
                                      cAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCCACCTccgcc                                                               >  1:2206996/1‑98 (MQ=255)
                                      cAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTccgcc                                                               >  1:369240/1‑98 (MQ=255)
                                      cAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTccgcc                                                               <  2:1709296/101‑4 (MQ=255)
                                       aGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGCGGCGACAAAATTCTT.CCCCGGCGGTTCCGCCCTCGGCGGGGCGCAACCTgccccg                                                              >  2:1692491/1‑98 (MQ=255)
                                           tGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTcc                                                         >  2:2510528/1‑101 (MQ=255)
                                           tGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTcc                                                         >  1:2313721/1‑101 (MQ=255)
                                           tGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTcc                                                         >  1:234026/1‑101 (MQ=255)
                                            gAACCCTTTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTccc                                                        <  2:3724284/101‑1 (MQ=255)
                                            gAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCCACCT.CCGCCGGTccc                                                        >  1:3515781/1‑101 (MQ=255)
                                            gAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTccc                                                        >  1:84979/1‑101 (MQ=255)
                                             accccATTGTTTTTCCCGCTCTTTAGCCGATGGCCGGGGGGTCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTccca                                                       <  2:3828673/99‑2 (MQ=255)
                                             aaCCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTccca                                                       <  2:285605/101‑2 (MQ=255)
                                             aaCCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTccca                                                       >  1:2758042/1‑100 (MQ=255)
                                              aCCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTcccag                                                      <  2:1335506/101‑3 (MQ=255)
                                                ccATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTcccagcg                                                    <  1:2103829/101‑5 (MQ=255)
                                                 cATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCgg                                                  <  1:191255/101‑1 (MQ=255)
                                                    tGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCggggg                                               <  1:1645357/101‑1 (MQ=255)
                                                    tGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCggggg                                               >  1:320634/1‑101 (MQ=255)
                                                     ggCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGa                                              >  1:1358306/1‑101 (MQ=255)
                                                     ggCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGa                                              >  2:2068491/1‑101 (MQ=255)
                                                      gCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGAc                                             <  2:1776097/101‑1 (MQ=255)
                                                      gCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGAGGGGGAc                                             <  1:1206490/101‑1 (MQ=255)
                                                       cTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACt                                            >  1:3133502/1‑101 (MQ=255)
                                                        ttttCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACtg                                           >  2:143852/1‑101 (MQ=255)
                                                         tttCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACtgt                                          <  2:369189/101‑1 (MQ=255)
                                                          ttCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACtgtg                                         >  1:4091368/1‑101 (MQ=255)
                                                           tCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTgg                                        >  1:3295071/1‑101 (MQ=255)
                                                            ccTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGc                                       >  2:2129804/1‑101 (MQ=255)
                                                            ccTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGGggg                                       >  1:553078/1‑100 (MQ=255)
                                                            ccTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAAACT.CCGCCGGT.CCCAGCgggggccgttggc                                       >  1:3369006/1‑93 (MQ=255)
                                                             cTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCa                                      >  2:1451084/1‑101 (MQ=255)
                                                              tGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCaa                                     <  1:3913402/101‑1 (MQ=255)
                                                               gTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCaaa                                    <  2:3098165/101‑1 (MQ=255)
                                                               gTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCaaa                                    <  1:46546/101‑1 (MQ=255)
                                                                  tctAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCt                                 <  2:4159706/101‑1 (MQ=255)
                                                                  tctAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCt                                 <  1:453088/101‑1 (MQ=255)
                                                                   ctAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGGCTGTGGCAAAGCTg                                >  1:1623434/1‑101 (MQ=255)
                                                                   ctAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTg                                <  2:2971411/101‑1 (MQ=255)
                                                                    tAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGACGCAAAGCTGc                               >  2:2703573/1‑101 (MQ=255)
                                                                     aGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGca                              >  2:2900561/1‑101 (MQ=255)
                                                                     aGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGca                              <  1:956230/101‑1 (MQ=255)
                                                                       ccGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGcacc                            <  1:2882639/101‑1 (MQ=255)
                                                                        cGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGcacca                           <  2:835224/101‑1 (MQ=255)
                                                                         gATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGcaccac                          >  2:1814050/1‑101 (MQ=255)
                                                                         gATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGcaccac                          <  2:1276379/101‑1 (MQ=255)
                                                                          aTGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGGCTGTGGCAAAGCTGgaacacc                         >  2:3608357/1‑100 (MQ=255)
                                                                            ggCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CTCAGCGGGGGACTGTGGCATAGCTGCACCAcgcg                       >  1:3650479/1‑101 (MQ=255)
                                                                                gggggggCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGACAGCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCCAAGCTGCACCACGCGGttt                   >  2:3993779/1‑101 (MQ=255)
                                                                                 ggggggCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTc                  <  1:375602/101‑1 (MQ=255)
                                                                                 ggggggCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGGTTc                  >  1:2974737/1‑101 (MQ=255)
                                                                                  gggggCGACAAGATTTTT.CCCCCGCGGGGCCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCg                 <  2:1146437/101‑1 (MQ=255)
                                                                                  gggggCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCg                 >  1:2602822/1‑101 (MQ=255)
                                                                                     ggCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCtt              >  1:1672705/1‑101 (MQ=255)
                                                                                     ggCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCtt              >  2:3518633/1‑101 (MQ=255)
                                                                                     ggCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCtt              <  1:781218/101‑1 (MQ=255)
                                                                                     ggCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCtt              <  2:2779010/101‑1 (MQ=255)
                                                                                      gCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCttt             >  2:72707/1‑101 (MQ=255)
                                                                                         aCAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGc          <  1:2129949/101‑1 (MQ=255)
                                                                                         aCAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGc          >  2:1562314/1‑101 (MQ=255)
                                                                                          cAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGcc         <  2:2286624/101‑1 (MQ=255)
                                                                                           aaGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCg        <  1:1033593/101‑1 (MQ=255)
                                                                                           aaGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGG.CCCCAGCGGGGGACTGTGGCACAGCTGCCCCACGCGGTTTCGCTTTGCccc        >  1:3413913/1‑100 (MQ=255)
                                                                                            aagattttCCCCCCGCGGGACCGCCCGCGGCGGGGCGCACCCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCg        <  2:597123/97‑1 (MQ=255)
                                                                                              gattttTCCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCGCt      >  2:1023851/3‑101 (MQ=255)
                                                                                              gattttTCCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCAGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCGCt      >  1:3558810/3‑101 (MQ=255)
                                                                                               attttTCCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCGCtt     >  1:272384/2‑101 (MQ=255)
                                                                                               attttCCCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCGCtt     <  1:1712304/100‑1 (MQ=255)
                                                                                                ttttTCCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCGCTTc    <  1:3073699/101‑1 (MQ=255)
                                                                                                 tttGCCCCCGCGGCCCCGCCCGCGGCGGGAAGAAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCCCGCGGTTTCGCTTTGGCCGCTTCg   <  2:553014/101‑1 (MQ=255)
                                                                                                                                                                                                         
CAAAGAGGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTCCCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTCCCGCCGGTCCCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCGCTTCGG  >  NC_014034/296180‑296380

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG