Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,171 +C 100% coding (1314/1734 nt) recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,1711.→C100.0% 411.3 109coding (1314/1734 nt)recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (57/52):  ref base (0/0):  total (57/52)

Only 100 of 135 total aligned reads displayed.

GGGGGTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCC.GAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGAG.TGTTCGCCCGGCAGGGCGC.GG..CAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGG  >  NC_014034/3256072‑3256255
                                                                                                      |                                                                                              
gggggTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAgg                                                                                                 >  1:1015679/1‑101 (MQ=255)
gggggTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAgg                                                                                                 >  1:3665932/1‑101 (MQ=255)
gggggTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAgg                                                                                                 >  2:1096845/1‑101 (MQ=255)
gggggTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAgg                                                                                                 >  2:3487194/1‑101 (MQ=255)
 ggggTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAggc                                                                                                >  1:770205/1‑100 (MQ=255)
  gggTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAggcg                                                                                               >  1:2339106/1‑99 (MQ=255)
  gggTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAggcg                                                                                               <  2:4081885/101‑3 (MQ=255)
  gggTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAggcg                                                                                               <  1:2176315/101‑3 (MQ=255)
   ggTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAggcgc                                                                                              <  1:3601158/101‑4 (MQ=255)
   ggTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAggcgc                                                                                              >  1:781117/1‑98 (MQ=255)
   ggTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAggcgc                                                                                              <  2:3643109/101‑4 (MQ=255)
    gTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAggcgca                                                                                             <  2:873158/101‑5 (MQ=255)
    gTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAggcgca                                                                                             <  1:129036/101‑5 (MQ=255)
     tCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAggcgcag                                                                                            <  1:121897/101‑6 (MQ=255)
       gATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAggcgcagct                                                                                          >  2:3890863/1‑94 (MQ=255)
         tCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAggcgcagctgg                                                                                        <  1:257045/101‑10 (MQ=255)
          cTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAggcgcagctgga                                                                                       <  1:1994740/101‑11 (MQ=255)
              gcgcGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAA.ccc                                                                                  <  1:2663616/101‑2 (MQ=255)
              gcgcGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAA.ccc                                                                                  <  2:1436003/101‑2 (MQ=255)
               cgcgGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAA.cccg                                                                                 <  2:2087849/101‑3 (MQ=255)
               cgcgGCGCTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAATGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAA.cccg                                                                                 >  2:2224565/1‑99 (MQ=255)
                gcggcgGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGGCGCGGGGCTGACCCTGGCC.GAGGCGCAGCTGGAA.cccgc                                                                                >  2:517274/1‑98 (MQ=255)
                   gcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGAt                                                                              <  2:580878/101‑1 (MQ=255)
                   gcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGAt                                                                              <  2:181539/101‑1 (MQ=255)
                    cggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATg                                                                             >  2:3972211/1‑101 (MQ=255)
                    cggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATg                                                                             >  1:3831088/1‑101 (MQ=255)
                    cggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATg                                                                             <  2:3140485/101‑1 (MQ=255)
                    cggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATg                                                                             >  2:950390/1‑101 (MQ=255)
                    cggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATg                                                                             >  1:1806203/1‑101 (MQ=255)
                     ggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATgg                                                                            >  2:2848773/1‑101 (MQ=255)
                      gTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATggc                                                                           <  1:2365595/101‑2 (MQ=255)
                      gTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATggc                                                                           <  1:1415792/101‑2 (MQ=255)
                      gTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATggc                                                                           <  1:1901283/101‑2 (MQ=255)
                        gCAGCGCGTCGCGGGCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATggcac                                                                         <  2:448859/101‑4 (MQ=255)
                        gCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATggcac                                                                         >  1:3367206/1‑98 (MQ=255)
                        gCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATggcac                                                                         >  2:2503809/1‑98 (MQ=255)
                        gCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATggcac                                                                         <  1:1666967/101‑4 (MQ=255)
                          aGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATggcacgg                                                                       <  2:2639446/101‑6 (MQ=255)
                            cgcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATggcacggct                                                                     <  2:522620/101‑8 (MQ=255)
                             gcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATggcacggctg                                                                    <  2:3021663/101‑9 (MQ=255)
                              cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATggcacggctgg                                                                   >  1:3256455/1‑92 (MQ=255)
                              cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATggcacggctgg                                                                   >  1:3365151/1‑92 (MQ=255)
                              cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATggcacggctgg                                                                   <  1:2656973/101‑10 (MQ=255)
                               gTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATggcacggctggc                                                                  >  2:1588843/1‑91 (MQ=255)
                               gTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATggcacggctggc                                                                  <  2:3818094/101‑11 (MQ=255)
                               gTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATggcacggctggc                                                                  >  1:1111058/1‑91 (MQ=255)
                                    ggCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGGC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.t                                                           <  1:1118370/101‑1 (MQ=255)
                                    ggCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.t                                                           >  1:3789868/1‑101 (MQ=255)
                                     gCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.tt                                                          >  1:1238754/1‑100 (MQ=255)
                                      ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttg                                                         >  1:6896/1‑99 (MQ=255)
                                         aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgctc                                                      >  2:3557312/1‑96 (MQ=255)
                                         aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgctc                                                      >  2:2781199/1‑96 (MQ=255)
                                            ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgctcgcc                                                   <  2:3366961/101‑9 (MQ=255)
                                              cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCg                                                  >  1:4071809/1‑101 (MQ=255)
                                              cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCg                                                  <  1:1544743/101‑1 (MQ=255)
                                              cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCg                                                  >  2:1062978/1‑101 (MQ=255)
                                                tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGc                                                >  1:2168263/1‑101 (MQ=255)
                                                   gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAgg                                             >  1:54871/1‑101 (MQ=255)
                                                   gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAgg                                             >  1:4089221/1‑101 (MQ=255)
                                                   gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAgg                                             >  2:886788/1‑101 (MQ=255)
                                                   gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCCGCAgg                                             >  2:2886541/1‑101 (MQ=255)
                                                    cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAggg                                            <  2:2578997/101‑1 (MQ=255)
                                                    cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAggg                                            >  1:3155912/1‑101 (MQ=255)
                                                     tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCCGGgc                                           >  2:189759/1‑101 (MQ=255)
                                                      cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAggggg                                          >  1:1729782/1‑99 (MQ=255)
                                                      cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGgcg                                          <  2:3256214/101‑1 (MQ=255)
                                                      cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCCGCAggggc                                          >  2:3239811/1‑99 (MQ=255)
                                                      cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCCCCCGGCAGGgcg                                          >  2:2242729/1‑101 (MQ=255)
                                                      cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGAGTTGCTCGCCCGGCAGGgcg                                          <  2:3364906/101‑1 (MQ=255)
                                                       aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGgcgc                                         <  2:1097874/101‑1 (MQ=255)
                                                        aGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGAGTTGCTCGCCCGGCAGGGcgc.g                                       <  1:612609/101‑1 (MQ=255)
                                                        aGGGCGGCGGGCACCGCATGGCCCCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCCCCCGGCCGGGggc.g                                       >  1:1586314/1‑101 (MQ=255)
                                                             ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGGGC.gggcac                                  >  1:2476887/1‑97 (MQ=255)
                                                              gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGGGC.ggggacg                                 >  2:1642305/1‑96 (MQ=255)
                                                              gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGC.gggcacg                                 <  2:873799/101‑6 (MQ=255)
                                                               cggGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGG..CAc.gg                              <  1:3914675/101‑1 (MQ=255)
                                                                 ggCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGG..CAc.gggc                            <  2:1806084/101‑3 (MQ=255)
                                                                 ggCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGG..CAc.gggc                            <  1:4126689/101‑3 (MQ=255)
                                                                  gCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGGGC.ggggacggggg                             >  2:3437015/1‑92 (MQ=255)
                                                                   cACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCCGCAGGGCCCGGG..CAc.gggcgc                          >  1:2839833/1‑97 (MQ=255)
                                                                       gCAGGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGG..CACGGGcggcccg                       <  2:1460536/101‑1 (MQ=255)
                                                                        cATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGG..CACGGGcggcccgg                      <  1:3147540/101‑1 (MQ=255)
                                                                         gtGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGG..CACGGGcggcccggc                     <  1:3972471/100‑1 (MQ=255)
                                                                         aTGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGG..CACGGGcggcccggc                     >  1:1649584/1‑101 (MQ=255)
                                                                              cgcgGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGG..CACGGGcggcccggcccgtc                >  1:3735473/1‑97 (MQ=255)
                                                                              cgcgGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGG..CACGGGCGGCCCGGCCGATc                <  1:256926/101‑1 (MQ=255)
                                                                               gcgGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGG..CACGGGGGGCCCGGGCGATcg               >  2:2744987/1‑100 (MQ=255)
                                                                                 gggTCTGACCCT.GGCAGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGG..GACGGGCGGCCCGGCCGGTCttc             >  2:4043323/1‑99 (MQ=255)
                                                                                  ggTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGG..CACGGGCGGGCCCGCCGATCTGCg            >  2:1655125/1‑101 (MQ=255)
                                                                                     cTGCCCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGG..CACGGGCGGCCCGGCCGATCTGCGGCt         <  1:950475/101‑1 (MQ=255)
                                                                                      tGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGG..CACGGGGGGGCCGGCCGGTCTGCGGGTg        >  2:2259703/1‑101 (MQ=255)
                                                                                      tGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGG..CACGGGCGGCCCGGCCGATCTGCGGCTg        >  1:3926522/1‑101 (MQ=255)
                                                                                       gcccctggCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGG..CACGGGCGGCCCGGCCGATCTGCGGCTgg       <  1:876833/94‑1 (MQ=255)
                                                                                       gACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGG..CACGGGCGGCCCGGTCGATCGGCGGCGgg       >  1:3656888/1‑101 (MQ=255)
                                                                                         ccctggCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGG..CACGGGCGGCCCGGCCGCTCTGCGGCTgggg     >  1:3701739/6‑99 (MQ=255)
                                                                                         ccctggCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGAc     <  1:189797/96‑1 (MQ=255)
                                                                                         ccctggCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGAc     <  2:1238658/96‑1 (MQ=255)
                                                                                          ccgggCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACg    <  2:4032150/97‑1 (MQ=255)
                                                                                           ctggCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGG..GACGGGCGGCCCGGCCGGTCTGCGGCTgggagg   >  1:3007456/4‑97 (MQ=255)
                                                                                            tggCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGC.GGGCCCC.GGCGGCCCGGCCGGTTCGCGGCttgcgtgg  >  2:1287556/3‑94 (MQ=255)
                                                                                                      |                                                                                              
GGGGGTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCC.GAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGAG.TGTTCGCCCGGCAGGGCGC.GG..CAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGG  >  NC_014034/3256072‑3256255

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG