BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,256,180	+C	100%	coding (1323/1734 nt)	recJ →	single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,256,180	1	.→C	100.0%	444.6	118	coding (1323/1734 nt)	recJ	single‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand): new base (64/54): ref base (0/0): total (64/54)

Only 100 of 131 total aligned reads displayed.


GCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCC.GAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGAG.TGTTCGCCCGGCAGGGCG.C.GG..CAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGC  >  NC_014034/3256086‑3256270


gcgcGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAA.ccc                                                                                                  <  1:2663616/101‑2 (MQ=255)


gcgcGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAA.ccc                                                                                                  <  2:1436003/101‑2 (MQ=255)


 cgcgGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAA.cccg                                                                                                 <  2:2087849/101‑3 (MQ=255)


   cggcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAA.cccgcg                                                                                               >  2:1134952/1‑97 (MQ=255)


   cggcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAA.cccgcg                                                                                               <  2:3264907/101‑5 (MQ=255)


   cggcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAA.cccgcg                                                                                               <  2:1446022/101‑5 (MQ=255)


     gcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGAt                                                                                              <  2:580878/101‑1 (MQ=255)


     gcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGAt                                                                                              <  2:3646077/101‑1 (MQ=255)


     gcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGAt                                                                                              <  2:181539/101‑1 (MQ=255)


      cggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATg                                                                                             >  2:950390/1‑101 (MQ=255)


      cggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATg                                                                                             >  2:3972211/1‑101 (MQ=255)


      cggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATg                                                                                             <  2:3140485/101‑1 (MQ=255)


      cggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATg                                                                                             >  1:1806203/1‑101 (MQ=255)


       ggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATgg                                                                                            >  2:2848773/1‑101 (MQ=255)


        gTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATggc                                                                                           <  1:2365595/101‑2 (MQ=255)


        gTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATggc                                                                                           <  1:1415792/101‑2 (MQ=255)


          gCAGCGCGTCGCGGGCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATggcac                                                                                         <  2:448859/101‑4 (MQ=255)


          gCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATggcac                                                                                         >  2:2503809/1‑98 (MQ=255)


          gCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATggcac                                                                                         <  1:1666967/101‑4 (MQ=255)


          gCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATggcac                                                                                         >  1:3367206/1‑98 (MQ=255)


            aGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATggcacgg                                                                                       <  2:2639446/101‑6 (MQ=255)


            aGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATggcacgg                                                                                       >  1:3818351/1‑96 (MQ=255)


              ggcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATggcacggct                                                                                     <  1:2975194/100‑8 (MQ=255)


              cgcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATggcacggct                                                                                     <  2:522620/101‑8 (MQ=255)


                cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATggcacggctgg                                                                                   <  1:2656973/101‑10 (MQ=255)


                cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATggcacggctgg                                                                                   >  1:3256455/1‑92 (MQ=255)


                cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATggcacggctgg                                                                                   >  1:3365151/1‑92 (MQ=255)


                 gTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATggcacggctggc                                                                                  >  2:1588843/1‑91 (MQ=255)


                 gTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATggcacggctggc                                                                                  >  1:1111058/1‑91 (MQ=255)


                      ggCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGGC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.t                                                                           <  1:1118370/101‑1 (MQ=255)


                      ggCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.t                                                                           >  2:10517/1‑101 (MQ=255)


                      ggCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.t                                                                           >  1:3789868/1‑101 (MQ=255)


                       gCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.tt                                                                          >  1:1343364/1‑100 (MQ=255)


                        ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttg                                                                         >  1:6896/1‑99 (MQ=255)


                           aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgctc                                                                      >  2:2781199/1‑96 (MQ=255)


                           aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgctc                                                                      >  2:3557312/1‑96 (MQ=255)


                              ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgctcgcc                                                                   >  2:3481095/1‑93 (MQ=255)


                                cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCg                                                                  <  2:1593111/101‑1 (MQ=255)


                                cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCg                                                                  <  2:2849962/101‑1 (MQ=255)


                                cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCg                                                                  >  1:4071809/1‑101 (MQ=255)


                                cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCg                                                                  <  1:1544743/101‑1 (MQ=255)


                                  tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGc                                                                >  2:2670519/1‑101 (MQ=255)


                                  tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGc                                                                >  1:2168263/1‑101 (MQ=255)


                                     gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAgg                                                             >  1:4089221/1‑101 (MQ=255)


                                     gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAgg                                                             >  1:54871/1‑101 (MQ=255)


                                     gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAgg                                                             >  2:886788/1‑101 (MQ=255)


                                     gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCCGCAgg                                                             >  2:2886541/1‑101 (MQ=255)


                                      cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAggg                                                            <  2:2578997/101‑1 (MQ=255)


                                      cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAggg                                                            >  1:3155912/1‑101 (MQ=255)


                                       tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCCGGgc                                                           >  2:189759/1‑101 (MQ=255)


                                       ccAAGGGCGGCGGGCCCCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGgc                                                           <  1:1197431/100‑1 (MQ=255)


                                        cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAggggg                                                          >  2:3984104/1‑99 (MQ=255)


                                        cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGgcg                                                          <  2:3256214/101‑1 (MQ=255)


                                        cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCCCCCGGCAGGgcg                                                          >  2:2242729/1‑101 (MQ=255)


                                        cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGAGTTGCTCGCCCGGCAGGgcg                                                          <  2:3364906/101‑1 (MQ=255)


                                         aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGgcg.c                                                        <  2:1097874/101‑1 (MQ=255)


                                          aGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGAGTTGCTCGCCCGGCAGGGcg.c.g                                                      <  1:612609/101‑1 (MQ=255)


                                           gggCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.C.gg                                                     <  2:3236532/101‑1 (MQ=255)


                                           ggacggtggGCACCGCATGGCCGCCGGTCGGACCCTGGCC.GAGGCGCAGCTGGAATCAGGGATGGCAC.GGCTGGCCGAGTTGCTCGCGCGGCAGGGCG.C.gg                                                     <  2:4124824/98‑1 (MQ=255)


                                               ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGGG.C.gggcac                                                 >  1:2476887/1‑97 (MQ=255)


                                                gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGGG.C.ggggacg                                                >  2:1642305/1‑96 (MQ=255)


                                                gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.C.gggcacg                                                <  2:873799/101‑6 (MQ=255)


                                                 cggGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CAc.gg                                             <  1:3914675/101‑1 (MQ=255)


                                                  gggCCCCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CAc.ggg                                            <  1:3681722/101‑2 (MQ=255)


                                                   ggCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CAc.gggc                                           <  2:1806084/101‑3 (MQ=255)


                                                    gCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGGG.C.ggggacggggg                                            >  2:3437015/1‑92 (MQ=255)


                                                     cACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGGG.CGGG..CAc.gggcgg                                         >  2:1809786/1‑97 (MQ=255)


                                                     cACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CAc.gggcgg                                         <  1:549396/101‑5 (MQ=255)


                                                       ccGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGcggcc                                        <  2:2183027/101‑1 (MQ=255)


                                                           aTGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGcggcccggc                                    >  1:1649584/1‑101 (MQ=255)


                                                           aTGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGcggcccggc                                    <  1:1588952/101‑1 (MQ=255)


                                                            tGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGcgggccggcc                                   >  2:540962/1‑101 (MQ=255)


                                                             ggCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCg                                  <  1:1741761/101‑1 (MQ=255)


                                                              gCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCGGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCC.AG..CACGGGCGGCCCGGCCGa                                 >  1:2394304/1‑101 (MQ=255)


                                                                cgcgGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGcggcccggcccgtc                               >  1:3735473/1‑97 (MQ=255)


                                                                 gcgGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGGGGCCCGGGCGATcg                              >  2:2744987/1‑100 (MQ=255)


                                                                   gggTCTGACCCT.GGCAGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..GACGGGCGGCCCGGCCGGTCttc                            >  2:4043323/1‑99 (MQ=255)


                                                                     gTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCgg                          <  1:1901779/101‑1 (MQ=255)


                                                                       cTGCCCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCt                        <  1:950475/101‑1 (MQ=255)


                                                                        tGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGGGGGCCGGCCGGTCTGCGGGTg                       >  2:2259703/1‑101 (MQ=255)


                                                                        tGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTg                       >  1:3926522/1‑101 (MQ=255)


                                                                         gACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGTCGATCGGCGGCGgg                      >  1:3656888/1‑101 (MQ=255)


                                                                         gACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCCGCCCGGCCGATCTGCGGCTgg                      >  1:2649460/1‑101 (MQ=255)


                                                                           ccctggCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGAc                    <  1:189797/96‑1 (MQ=255)


                                                                             ctggCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..GACGGGCGGCCCGGCCGGTCTGCGGCTgggagg                  >  1:3007456/4‑97 (MQ=255)


                                                                              tggCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGGTCTGCGGCTGGGCggg                 >  1:4073709/3‑101 (MQ=255)


                                                                              tggCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.C.GGGCCCC.GGCGGCCCGGCCGGTTCGCGGCttgcgtgg                 >  2:1287556/3‑94 (MQ=255)


                                                                                    gaggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCt            <  1:2314555/91‑1 (MQ=255)


                                                                                     aggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGCTCTGGGGCTCGACGGGCTCCtc           >  2:3110736/10‑100 (MQ=255)


                                                                                      ggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGGGGCCCGGCCGGTCTGCGGCTGGGCGGGGGCCTgg          >  2:1037200/9‑100 (MQ=255)


                                                                                       gcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAt         <  2:2735074/94‑1 (MQ=255)


                                                                                        cgcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGGTCTGCGGCTGGGCGGGGGCCCGATg        >  2:1105499/7‑101 (MQ=255)


                                                                                         gcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGc       <  2:2338938/96‑1 (MQ=255)


                                                                                          cagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGcc      <  1:1096939/97‑1 (MQ=255)


                                                                                          cagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGAAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGcc      <  1:3557565/97‑1 (MQ=255)


                                                                                           agctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCg     <  2:1649469/98‑1 (MQ=255)


                                                                                            gctGGAACCCGCGATGG.CACGGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCCGATGcccg    >  1:3850544/3‑99 (MQ=255)


                                                                                             ctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CCCGGGCGGCCCGGGCGATCTGCGGCTGGGCGGGCTCCTGATGGCggg   >  1:201139/2‑101 (MQ=255)


                                                                                              tGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGGTCTGCGGCTGGGCGGGGTCCCGGTGCCgggg  >  1:3102891/1‑100 (MQ=255)


                                                                                              tGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGgc  <  2:3850285/101‑1 (MQ=255)


GCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCC.GAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGAG.TGTTCGCCCGGCAGGGCG.C.GG..CAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGC  >  NC_014034/3256086‑3256270

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 29 ≤


ATCG

< 34 ≤


ATCG

< 40 ≤


ATCG