Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,195 +G 100% coding (1338/1734 nt) recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,1951.→G100.0% 401.4 106coding (1338/1734 nt)recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (58/48):  ref base (0/0):  total (58/48)

Only 100 of 107 total aligned reads displayed.

GGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCC.GAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGAG.TGTTCGCCCGGCAGGGCG.C.GG..CAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAC  >  NC_014034/3256108‑3256278
                                                                                               |                                                                                         
ggCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGGC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.t                                                                                   <  1:1118370/101‑1 (MQ=255)
ggCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.t                                                                                   >  2:10517/1‑101 (MQ=255)
ggCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.t                                                                                   >  1:3789868/1‑101 (MQ=255)
 gCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.tt                                                                                  >  1:1238754/1‑100 (MQ=255)
 gCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.tt                                                                                  >  1:1343364/1‑100 (MQ=255)
  ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttg                                                                                 >  1:6896/1‑99 (MQ=255)
     aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgctc                                                                              >  2:2781199/1‑96 (MQ=255)
     aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgctc                                                                              >  2:3557312/1‑96 (MQ=255)
        ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgctcgcc                                                                           <  2:3366961/101‑9 (MQ=255)
        ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgctcgcc                                                                           >  2:3481095/1‑93 (MQ=255)
          cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCg                                                                          <  2:2849962/101‑1 (MQ=255)
          cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCg                                                                          <  2:2433355/101‑1 (MQ=255)
          cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCg                                                                          <  2:1593111/101‑1 (MQ=255)
          cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCg                                                                          >  2:1062978/1‑101 (MQ=255)
          cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCg                                                                          >  1:4071809/1‑101 (MQ=255)
          cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCg                                                                          <  1:1544743/101‑1 (MQ=255)
            tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGc                                                                        >  2:2670519/1‑101 (MQ=255)
               gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAgg                                                                     >  2:886788/1‑101 (MQ=255)
               gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAgg                                                                     >  1:54871/1‑101 (MQ=255)
               gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAgg                                                                     >  1:4089221/1‑101 (MQ=255)
               gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCCGCAgg                                                                     >  2:2886541/1‑101 (MQ=255)
                cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAggg                                                                    >  1:3155912/1‑101 (MQ=255)
                 tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCCGGgc                                                                   >  2:189759/1‑101 (MQ=255)
                 ccAAGGGCGGCGGGCCCCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGgc                                                                   <  1:1197431/100‑1 (MQ=255)
                  cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAggggg                                                                  >  1:1729782/1‑99 (MQ=255)
                  cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAggggg                                                                  >  2:3984104/1‑99 (MQ=255)
                  cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGgcg                                                                  <  2:3256214/101‑1 (MQ=255)
                  cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCCGCAggggc                                                                  >  2:3239811/1‑99 (MQ=255)
                  cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCCCCCGGCAGGgcg                                                                  >  2:2242729/1‑101 (MQ=255)
                  cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGAGTTGCTCGCCCGGCAGGgcg                                                                  <  2:3364906/101‑1 (MQ=255)
                   aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGgcg.c                                                                <  2:1097874/101‑1 (MQ=255)
                    aGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGAGTTGCTCGCCCGGCAGGGcg.c.g                                                              <  1:612609/101‑1 (MQ=255)
                    aGGGCGGCGGGCACCGCATGGCCCCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCCCCCGGCCGGGgg.c.g                                                              >  1:1586314/1‑101 (MQ=255)
                     gggCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.C.gg                                                             <  2:3236532/101‑1 (MQ=255)
                     ggacggtggGCACCGCATGGCCGCCGGTCGGACCCTGGCC.GAGGCGCAGCTGGAATCAGGGATGGCAC.GGCTGGCCGAGTTGCTCGCGCGGCAGGGCG.C.gg                                                             <  2:4124824/98‑1 (MQ=255)
                       gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.C.gggc                                                           <  1:2315561/101‑3 (MQ=255)
                         ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGGG.C.gggcac                                                         >  1:2476887/1‑97 (MQ=255)
                         ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.C.gggcac                                                         <  1:354214/101‑5 (MQ=255)
                          gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGGG.C.ggggacg                                                        >  2:1642305/1‑96 (MQ=255)
                          gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.C.gggcacg                                                        <  2:873799/101‑6 (MQ=255)
                           cggGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CAc.gg                                                     <  1:3914675/101‑1 (MQ=255)
                            gggCCCCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CAc.ggg                                                    <  1:3681722/101‑2 (MQ=255)
                             ggCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CAc.gggc                                                   <  2:1806084/101‑3 (MQ=255)
                             ggCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CAc.gggc                                                   <  1:4126689/101‑3 (MQ=255)
                              gCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGGG.C.ggggacggggg                                                    >  2:3437015/1‑92 (MQ=255)
                               cACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGGG.CGGG..CAc.gggcgg                                                 >  2:1809786/1‑97 (MQ=255)
                               cACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CAc.gggcgg                                                 <  1:549396/101‑5 (MQ=255)
                               cACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCCGCAGGGCC.CGGG..CAc.gggcgc                                                 >  1:2839833/1‑97 (MQ=255)
                                 ccGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGcggcc                                                <  2:2183027/101‑1 (MQ=255)
                                    cATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGcggcccgg                                             <  1:3147540/101‑1 (MQ=255)
                                     gtGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGcggcccggc                                            <  1:3972471/100‑1 (MQ=255)
                                     aTGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGcggcccggc                                            >  1:1649584/1‑101 (MQ=255)
                                     aTGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGcggcccggc                                            <  1:1588952/101‑1 (MQ=255)
                                      tGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGcgggccggcc                                           >  2:540962/1‑101 (MQ=255)
                                       ggCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCg                                          <  1:1741761/101‑1 (MQ=255)
                                        gCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCGGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCC.AG..CACGGGCGGCCCGGCCGa                                         >  1:2394304/1‑101 (MQ=255)
                                          cgcgGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGcggcccggcccgtc                                       >  1:3735473/1‑97 (MQ=255)
                                          cgcgGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATc                                       <  1:256926/101‑1 (MQ=255)
                                           gcgGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGGGGCCCGGGCGATcg                                      >  2:2744987/1‑100 (MQ=255)
                                             gggTCTGACCCT.GGCAGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..GACGGGCGGCCCGGCCGGTCttc                                    >  2:4043323/1‑99 (MQ=255)
                                              ggTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGGCCCGCCGATCTGCg                                   >  2:1655125/1‑101 (MQ=255)
                                               gTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCgg                                  <  1:1901779/101‑1 (MQ=255)
                                                 cTGCCCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCt                                <  1:950475/101‑1 (MQ=255)
                                                  tGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGGGGGCCGGCCGGTCTGCGGGTg                               >  2:2259703/1‑101 (MQ=255)
                                                  tGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTg                               >  1:3926522/1‑101 (MQ=255)
                                                   gcccctggCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTgg                              <  1:876833/94‑1 (MQ=255)
                                                   gACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGTCGATCGGCGGCGgg                              >  1:3656888/1‑101 (MQ=255)
                                                   gACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCCGCCCGGCCGATCTGCGGCTgg                              >  1:2649460/1‑101 (MQ=255)
                                                     ccctggCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGCTCTGCGGCTgggg                            >  1:3701739/6‑99 (MQ=255)
                                                     ccctggCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGAc                            <  1:189797/96‑1 (MQ=255)
                                                     ccctggCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGAc                            <  2:1238658/96‑1 (MQ=255)
                                                      ccgggCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACg                           <  2:4032150/97‑1 (MQ=255)
                                                       ctggCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..GACGGGCGGCCCGGCCGGTCTGCGGCTgggagg                          >  1:3007456/4‑97 (MQ=255)
                                                        tggCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGGTCTGCGGCTGGGCggg                         >  1:4073709/3‑101 (MQ=255)
                                                        tggCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.C.GGGCCCC.GGCGGCCCGGCCGGTTCGCGGCttgcgtgg                         >  2:1287556/3‑94 (MQ=255)
                                                              gaggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCt                    <  1:2314555/91‑1 (MQ=255)
                                                               aggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGCTCTGGGGCTCGACGGGCTCCtc                   >  2:3110736/10‑100 (MQ=255)
                                                                ggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGGGGCCCGGCCGGTCTGCGGCTGGGCGGGGGCCTgg                  >  2:1037200/9‑100 (MQ=255)
                                                                 gcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAt                 <  2:2735074/94‑1 (MQ=255)
                                                                  cgcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGGTCTGCGGCTGGGCGGGGGCCCGATg                >  2:1105499/7‑101 (MQ=255)
                                                                   gcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGCTCTGCGGCTGGACGGGCTCCTCATGc               >  2:650115/6‑101 (MQ=255)
                                                                   gcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGc               <  2:2338938/96‑1 (MQ=255)
                                                                    cagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGcc              <  1:1096939/97‑1 (MQ=255)
                                                                     agctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCg             <  2:1649469/98‑1 (MQ=255)
                                                                      gctGGAACCCGCGATGG.CACGGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCCGATGcccg            >  1:3850544/3‑99 (MQ=255)
                                                                       ctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CCCGGGCGGCCCGGGCGATCTGCGGCTGGGCGGGCTCCTGATGGCggg           >  1:201139/2‑101 (MQ=255)
                                                                       ctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCggg           <  2:2785404/100‑1 (MQ=255)
                                                                       ctGGAACCCGCGATGG.CACGGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCCGCGGCTGGACGGGCTCCCGATGCCggg           >  2:2663229/2‑101 (MQ=255)
                                                                       ctGGAACCCGCGATGG.CACGGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCCGCCGATCTGCGGCTGGACCGGCTCCCGCTGccacg           >  2:1869062/2‑98 (MQ=255)
                                                                        tGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGGTCTGCGGCTGGGCGGGGTCCCGGTGCCgggg          >  1:3102891/1‑100 (MQ=255)
                                                                        tGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGgc          <  2:3850285/101‑1 (MQ=255)
                                                                         ggaacccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGGTCCTGATTCCGGgcg         >  2:1718188/6‑101 (MQ=255)
                                                                           aacccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc       <  2:811265/98‑1 (MQ=255)
                                                                            acccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgccg      <  1:2852853/99‑1 (MQ=255)
                                                                            acccGCGATGG.CACGGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCCCCTGATGCCGGGggccg      >  1:1146660/3‑101 (MQ=255)
                                                                             cccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgccgc     <  2:652101/100‑1 (MQ=255)
                                                                              ccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCCGGGGGcgcg    >  1:4119429/1‑101 (MQ=255)
                                                                              ccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCCGATGCCGGGGGCcgcg    >  2:1364834/1‑101 (MQ=255)
                                                                               cgcgATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGTCCTGCTGCCCGGCGCCGGGa   >  2:1027332/1‑101 (MQ=255)
                                                                                gcgATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAc  <  1:2910544/101‑1 (MQ=255)
                                                                                               |                                                                                         
GGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCC.GAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGAG.TGTTCGCCCGGCAGGGCG.C.GG..CAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAC  >  NC_014034/3256108‑3256278

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG