Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,201 +T 100% coding (1344/1734 nt) recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,2011.→T100.0% 395.1 104coding (1344/1734 nt)recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (52/52):  ref base (0/0):  total (52/52)

Only 100 of 118 total aligned reads displayed.

GGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCC.GAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGAG.TGTTCGCCCGGCAGGGCG.C.GG..CAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGA  >  NC_014034/3256108‑3256293
                                                                                                      |                                                                                                 
ggCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGGC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.t                                                                                                  <  1:1118370/101‑1 (MQ=255)
ggCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.t                                                                                                  >  1:3789868/1‑101 (MQ=255)
ggCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.t                                                                                                  >  2:10517/1‑101 (MQ=255)
 gCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.tt                                                                                                 >  1:1238754/1‑100 (MQ=255)
 gCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.tt                                                                                                 >  1:1343364/1‑100 (MQ=255)
  ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttg                                                                                                >  1:6896/1‑99 (MQ=255)
     aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgctc                                                                                             >  2:2781199/1‑96 (MQ=255)
     aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgctc                                                                                             >  2:3557312/1‑96 (MQ=255)
        ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgctcgcc                                                                                          >  2:3481095/1‑93 (MQ=255)
        ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgctcgcc                                                                                          <  2:3366961/101‑9 (MQ=255)
          cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCg                                                                                         <  2:1593111/101‑1 (MQ=255)
          cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCg                                                                                         <  2:2433355/101‑1 (MQ=255)
          cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCg                                                                                         <  2:2849962/101‑1 (MQ=255)
          cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCg                                                                                         >  2:1062978/1‑101 (MQ=255)
          cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCg                                                                                         >  1:4071809/1‑101 (MQ=255)
            tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGc                                                                                       >  1:2168263/1‑101 (MQ=255)
            tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGc                                                                                       >  2:2670519/1‑101 (MQ=255)
               gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAgg                                                                                    >  1:54871/1‑101 (MQ=255)
               gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAgg                                                                                    >  1:4089221/1‑101 (MQ=255)
               gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAgg                                                                                    >  2:886788/1‑101 (MQ=255)
               gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCCGCAgg                                                                                    >  2:2886541/1‑101 (MQ=255)
                cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAggg                                                                                   >  1:3155912/1‑101 (MQ=255)
                 ccAAGGGCGGCGGGCCCCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGgc                                                                                  <  1:1197431/100‑1 (MQ=255)
                  cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAggggg                                                                                 >  2:3984104/1‑99 (MQ=255)
                  cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAggggg                                                                                 >  1:1729782/1‑99 (MQ=255)
                  cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGgcg                                                                                 <  2:3256214/101‑1 (MQ=255)
                  cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCCGCAggggc                                                                                 >  2:3239811/1‑99 (MQ=255)
                  cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCCCCCGGCAGGgcg                                                                                 >  2:2242729/1‑101 (MQ=255)
                   aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGgcg.c                                                                               <  2:1097874/101‑1 (MQ=255)
                    aGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGAGTTGCTCGCCCGGCAGGGcg.c.g                                                                             <  1:612609/101‑1 (MQ=255)
                    aGGGCGGCGGGCACCGCATGGCCCCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCCCCCGGCCGGGgg.c.g                                                                             >  1:1586314/1‑101 (MQ=255)
                     gggCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.C.gg                                                                            <  2:3236532/101‑1 (MQ=255)
                     ggacggtggGCACCGCATGGCCGCCGGTCGGACCCTGGCC.GAGGCGCAGCTGGAATCAGGGATGGCAC.GGCTGGCCGAGTTGCTCGCGCGGCAGGGCG.C.gg                                                                            <  2:4124824/98‑1 (MQ=255)
                       gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.C.gggc                                                                          <  1:2315561/101‑3 (MQ=255)
                          gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGGG.C.ggggacg                                                                       >  2:1642305/1‑96 (MQ=255)
                          gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.C.gggcacg                                                                       <  2:873799/101‑6 (MQ=255)
                           cggGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CAc.gg                                                                    <  1:3914675/101‑1 (MQ=255)
                            gggCCCCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CAc.ggg                                                                   <  1:3681722/101‑2 (MQ=255)
                             ggCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CAc.gggc                                                                  <  2:1806084/101‑3 (MQ=255)
                             ggCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CAc.gggc                                                                  <  1:4126689/101‑3 (MQ=255)
                              gCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGGG.C.ggggacggggg                                                                   >  2:3437015/1‑92 (MQ=255)
                               cACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGGG.CGGG..CAc.gggcgg                                                                >  2:1809786/1‑97 (MQ=255)
                               cACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCCGCAGGGCC.CGGG..CAc.gggcgc                                                                >  1:2839833/1‑97 (MQ=255)
                                 ccGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGcggcc                                                               <  2:2183027/101‑1 (MQ=255)
                                   gCAGGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGcggcccg                                                             <  2:1460536/101‑1 (MQ=255)
                                    cATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGcggcccgg                                                            <  1:3147540/101‑1 (MQ=255)
                                     gtGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGcggcccggc                                                           <  1:3972471/100‑1 (MQ=255)
                                     aTGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGcggcccggc                                                           <  1:1588952/101‑1 (MQ=255)
                                     aTGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGcggcccggc                                                           >  1:1649584/1‑101 (MQ=255)
                                       ggCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCg                                                         <  1:1741761/101‑1 (MQ=255)
                                        gCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCGGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCC.AG..CACGGGCGGCCCGGCCGa                                                        >  1:2394304/1‑101 (MQ=255)
                                          cgcgGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGcggcccggcccgtc                                                      >  1:3735473/1‑97 (MQ=255)
                                          cgcgGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATc                                                      <  1:256926/101‑1 (MQ=255)
                                           gcgGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGGGGCCCGGGCGATcg                                                     >  2:2744987/1‑100 (MQ=255)
                                             gggTCTGACCCT.GGCAGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..GACGGGCGGCCCGGCCGGTCttc                                                   >  2:4043323/1‑99 (MQ=255)
                                              ggTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGGCCCGCCGATCTGCg                                                  >  2:1655125/1‑101 (MQ=255)
                                                 cTGCCCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCt                                               <  1:950475/101‑1 (MQ=255)
                                                  tGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGGGGGCCGGCCGGTCTGCGGGTg                                              >  2:2259703/1‑101 (MQ=255)
                                                  tGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTg                                              >  1:3926522/1‑101 (MQ=255)
                                                   gcccctggCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTgg                                             <  1:876833/94‑1 (MQ=255)
                                                   gACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGTCGATCGGCGGCGgg                                             >  1:3656888/1‑101 (MQ=255)
                                                   gACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCCGCCCGGCCGATCTGCGGCTgg                                             >  1:2649460/1‑101 (MQ=255)
                                                     ccctggCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGCTCTGCGGCTgggg                                           >  1:3701739/6‑99 (MQ=255)
                                                     ccctggCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGAc                                           <  1:189797/96‑1 (MQ=255)
                                                      ccgggCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACg                                          <  2:4032150/97‑1 (MQ=255)
                                                        tggCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.C.GGGCCCC.GGCGGCCCGGCCGGTTCGCGGCttgcgtgg                                        >  2:1287556/3‑94 (MQ=255)
                                                              gaggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCt                                   <  1:2314555/91‑1 (MQ=255)
                                                               aggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGCTCTGGGGCTCGACGGGCTCCtc                                  >  2:3110736/10‑100 (MQ=255)
                                                                ggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGGGGCCCGGCCGGTCTGCGGCTGGGCGGGGGCCTgg                                 >  2:1037200/9‑100 (MQ=255)
                                                                 gcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAt                                <  2:2735074/94‑1 (MQ=255)
                                                                  cgcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGGTCTGCGGCTGGGCGGGGGCCCGATg                               >  2:1105499/7‑101 (MQ=255)
                                                                   gcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGCTCTGCGGCTGGACGGGCTCCTCATGc                              >  2:650115/6‑101 (MQ=255)
                                                                   gcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGc                              <  2:2338938/96‑1 (MQ=255)
                                                                    cagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGcc                             <  1:1096939/97‑1 (MQ=255)
                                                                    cagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGAAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGcc                             <  1:3557565/97‑1 (MQ=255)
                                                                       ctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CCCGGGCGGCCCGGGCGATCTGCGGCTGGGCGGGCTCCTGATGGCggg                          >  1:201139/2‑101 (MQ=255)
                                                                       ctGGAACCCGCGATGG.CACGGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCCGCGGCTGGACGGGCTCCCGATGCCggg                          >  2:2663229/2‑101 (MQ=255)
                                                                       ctGGAACCCGCGATGG.CACGGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCCGCCGATCTGCGGCTGGACCGGCTCCCGCTGccacg                          >  2:1869062/2‑98 (MQ=255)
                                                                        tGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGGTCTGCGGCTGGGCGGGGTCCCGGTGCCgggg                         >  1:3102891/1‑100 (MQ=255)
                                                                        tGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGgc                         <  2:3850285/101‑1 (MQ=255)
                                                                         ggaacccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGGTCCTGATTCCGGgcg                        >  2:1718188/6‑101 (MQ=255)
                                                                           aacccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc                      <  1:500540/98‑1 (MQ=255)
                                                                           aacccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc                      <  2:811265/98‑1 (MQ=255)
                                                                           aacccGCGATGG.CACGGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc                      >  2:3424115/4‑101 (MQ=255)
                                                                            acccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgccg                     <  1:2852853/99‑1 (MQ=255)
                                                                            acccGCGATGG.CACGGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCCCCTGATGCCGGGggccg                     >  1:1146660/3‑101 (MQ=255)
                                                                             cccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgccgc                    <  2:652101/100‑1 (MQ=255)
                                                                              ccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCCGGGGGcgcg                   >  1:4119429/1‑101 (MQ=255)
                                                                              ccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCCGATGCCGGGGGCcgcg                   >  2:1364834/1‑101 (MQ=255)
                                                                                gcgATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAc                 <  1:2910544/101‑1 (MQ=255)
                                                                                      ggcacggctggCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCcg            <  2:1343264/91‑1 (MQ=255)
                                                                                        cacggctggCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCCGGCGCCGCGCCGCCcgcg          >  2:4176161/9‑101 (MQ=255)
                                                                                          cggctggCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCt        <  1:3442599/95‑1 (MQ=255)
                                                                                            gctggCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGa      <  1:2508983/97‑1 (MQ=255)
                                                                                             ctggCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGAt     >  1:3213100/4‑101 (MQ=255)
                                                                                              tggCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATc    >  1:4218318/3‑101 (MQ=255)
                                                                                              tggCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATc    <  1:692773/99‑1 (MQ=255)
                                                                                              tggCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATc    >  2:2008429/3‑101 (MQ=255)
                                                                                               ggCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGGTCCTGATGCCGGGCGCCGCGCCGCCCGCGCTGATCg   >  2:1062641/2‑101 (MQ=255)
                                                                                                gCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGa  <  1:4046142/101‑1 (MQ=255)
                                                                                                      |                                                                                                 
GGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCC.GAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGAG.TGTTCGCCCGGCAGGGCG.C.GG..CAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGA  >  NC_014034/3256108‑3256293

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG