Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,220 +G 100% coding (1363/1734 nt) recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,2201.→G100.0% 368.1 96coding (1363/1734 nt)recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (40/56):  ref base (0/0):  total (40/56)

Only 100 of 127 total aligned reads displayed.

AAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCC.GAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGAG.TGTTCGCCCGGCAGGGCG.C.GG..CAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGCCC  >  NC_014034/3256127‑3256314
                                                                                                        |                                                                                                 
aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGgcg.c                                                                                                    <  2:1097874/101‑1 (MQ=255)
 aGGGCGGCGGGCACCGCATGGCCCCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCCCCCGGCCGGGgg.c.g                                                                                                  >  1:1586314/1‑101 (MQ=255)
  gggCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.C.gg                                                                                                 <  2:3236532/101‑1 (MQ=255)
    gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.C.gggc                                                                                               <  1:2315561/101‑3 (MQ=255)
      ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGGG.C.gggcac                                                                                             >  1:2476887/1‑97 (MQ=255)
      ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.C.gggcac                                                                                             <  1:354214/101‑5 (MQ=255)
       gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGGG.C.ggggacg                                                                                            >  2:1642305/1‑96 (MQ=255)
        cggGCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CAc.gg                                                                                         <  1:3914675/101‑1 (MQ=255)
         gggCCCCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CAc.ggg                                                                                        <  1:3681722/101‑2 (MQ=255)
          ggCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CAc.gggc                                                                                       <  1:4126689/101‑3 (MQ=255)
          ggCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CAc.gggc                                                                                       <  2:1806084/101‑3 (MQ=255)
           gCACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGGG.C.ggggacggggg                                                                                        >  2:3437015/1‑92 (MQ=255)
            cACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGGG.CGGG..CAc.gggcgg                                                                                     >  2:1809786/1‑97 (MQ=255)
            cACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CAc.gggcgg                                                                                     <  1:549396/101‑5 (MQ=255)
            cACCGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCCGCAGGGCC.CGGG..CAc.gggcgc                                                                                     >  1:2839833/1‑97 (MQ=255)
              ccGCATGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGcggcc                                                                                    <  2:2183027/101‑1 (MQ=255)
                  gtGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGcggcccggc                                                                                <  1:3972471/100‑1 (MQ=255)
                  aTGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGcggcccggc                                                                                <  1:1588952/101‑1 (MQ=255)
                  aTGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGcggcccggc                                                                                >  1:1649584/1‑101 (MQ=255)
                   tGGCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGcgggccggcc                                                                               >  2:540962/1‑101 (MQ=255)
                    ggCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCg                                                                              <  1:1741761/101‑1 (MQ=255)
                     gCCGCGGGTCTGACCCTGGCC.GAGGCGCAGCGGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCC.AG..CACGGGCGGCCCGGCCGa                                                                             >  1:2394304/1‑101 (MQ=255)
                       cgcgGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGcggcccggcccgtc                                                                           >  1:3735473/1‑97 (MQ=255)
                        gcgGGTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGGGGCCCGGGCGATcg                                                                          >  2:2744987/1‑100 (MQ=255)
                          gggTCTGACCCT.GGCAGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..GACGGGCGGCCCGGCCGGTCttc                                                                        >  2:4043323/1‑99 (MQ=255)
                           ggTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGGCCCGCCGATCTGCg                                                                       >  2:1655125/1‑101 (MQ=255)
                            gTCTGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCgg                                                                      <  1:1901779/101‑1 (MQ=255)
                              cTGCCCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCt                                                                    <  1:950475/101‑1 (MQ=255)
                               tGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGGGGGCCGGCCGGTCTGCGGGTg                                                                   >  2:2259703/1‑101 (MQ=255)
                               tGACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTg                                                                   >  1:3926522/1‑101 (MQ=255)
                                gcccctggCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTgg                                                                  <  1:876833/94‑1 (MQ=255)
                                gACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGTCGATCGGCGGCGgg                                                                  >  1:3656888/1‑101 (MQ=255)
                                gACCCTGGCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCCGCCCGGCCGATCTGCGGCTgg                                                                  >  1:2649460/1‑101 (MQ=255)
                                  ccctggCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGAc                                                                <  2:1238658/96‑1 (MQ=255)
                                  ccctggCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGAc                                                                <  1:189797/96‑1 (MQ=255)
                                   ccgggCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACg                                                               <  2:4032150/97‑1 (MQ=255)
                                    ctggCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..GACGGGCGGCCCGGCCGGTCTGCGGCTgggagg                                                              >  1:3007456/4‑97 (MQ=255)
                                     tggCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGGTCTGCGGCTGGGCggg                                                             >  1:4073709/3‑101 (MQ=255)
                                     tggCC.GAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.C.GGGCCCC.GGCGGCCCGGCCGGTTCGCGGCttgcgtgg                                                             >  2:1287556/3‑94 (MQ=255)
                                            aggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGCTCTGGGGCTCGACGGGCTCCtc                                                       >  2:3110736/10‑100 (MQ=255)
                                             ggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGGGGCCCGGCCGGTCTGCGGCTGGGCGGGGGCCTgg                                                      >  2:1037200/9‑100 (MQ=255)
                                              gcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAt                                                     <  2:2735074/94‑1 (MQ=255)
                                               cgcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGGTCTGCGGCTGGGCGGGGGCCCGATg                                                    >  2:1105499/7‑101 (MQ=255)
                                                gcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGCTCTGCGGCTGGACGGGCTCCTCATGc                                                   >  2:650115/6‑101 (MQ=255)
                                                gcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGc                                                   <  2:2338938/96‑1 (MQ=255)
                                                 cagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGcc                                                  <  1:1096939/97‑1 (MQ=255)
                                                 cagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGAAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGcc                                                  <  1:3557565/97‑1 (MQ=255)
                                                  agctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCg                                                 <  2:1649469/98‑1 (MQ=255)
                                                   gctGGAACCCGCGATGG.CACGGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCCGATGcccg                                                >  1:3850544/3‑99 (MQ=255)
                                                    ctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCggg                                               <  2:2785404/100‑1 (MQ=255)
                                                    ctGGAACCCGCGATGG.CACGGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCCGCCGATCTGCGGCTGGACCGGCTCCCGCTGccacg                                               >  2:1869062/2‑98 (MQ=255)
                                                     tGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGGTCTGCGGCTGGGCGGGGTCCCGGTGCCgggg                                              >  1:3102891/1‑100 (MQ=255)
                                                     tGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGgc                                              <  2:3850285/101‑1 (MQ=255)
                                                        aacccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc                                           <  2:811265/98‑1 (MQ=255)
                                                        aacccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc                                           <  1:500540/98‑1 (MQ=255)
                                                        aacccGCGATGG.CACGGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc                                           >  2:3424115/4‑101 (MQ=255)
                                                         acccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgccg                                          <  1:2852853/99‑1 (MQ=255)
                                                           ccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCCGGGGGcgcg                                        >  1:4119429/1‑101 (MQ=255)
                                                           ccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCCGATGCCGGGGGCcgcg                                        >  2:1364834/1‑101 (MQ=255)
                                                            cgcgATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGTCCTGCTGCCCGGCGCCGGGa                                       >  2:1027332/1‑101 (MQ=255)
                                                             gcgATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAc                                      <  1:2910544/101‑1 (MQ=255)
                                                                   ggcacggctggCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCcg                                 <  2:1343264/91‑1 (MQ=255)
                                                                     cacggctggCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCCGGCGCCGCGCCGCCcgcg                               >  2:4176161/9‑101 (MQ=255)
                                                                       cggctggCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCt                             <  1:3442599/95‑1 (MQ=255)
                                                                         gctggCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGa                           <  1:2508983/97‑1 (MQ=255)
                                                                          ctggCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGAt                          >  1:3213100/4‑101 (MQ=255)
                                                                           tggCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATc                         >  1:4218318/3‑101 (MQ=255)
                                                                           tggCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATc                         <  1:692773/99‑1 (MQ=255)
                                                                           tggCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATc                         >  2:2008429/3‑101 (MQ=255)
                                                                             gCCGAGTTGCTCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGa                       <  1:4046142/101‑1 (MQ=255)
                                                                              ccgagttgctCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAg                      <  1:3891122/92‑1 (MQ=255)
                                                                               cgagttgctCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCGGGCGCCGCGGCGCCCCCCCTGATCGAgg                     >  2:73778/9‑101 (MQ=255)
                                                                                gagttgcccGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGa                    <  1:4176423/93‑1 (MQ=255)
                                                                                 agttgctCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAt                   <  2:1063031/95‑1 (MQ=255)
                                                                                   ttgctCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGTGCCGCGACGCCCGCGCTGATCGAGGAtct                 <  2:3830831/97‑1 (MQ=255)
                                                                                   ttgctCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAtct                 >  2:1624749/5‑101 (MQ=255)
                                                                                    tgctCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAtctc                >  1:3219225/4‑101 (MQ=255)
                                                                                    tgctCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAtctc                <  2:1756033/98‑1 (MQ=255)
                                                                                    tgctCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAtctc                <  2:2978157/98‑1 (MQ=255)
                                                                                    tgctCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAtctc                <  1:2257346/98‑1 (MQ=255)
                                                                                    tgctCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAtctc                >  2:1045550/4‑101 (MQ=255)
                                                                                     gctCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCg               <  1:2259862/99‑1 (MQ=255)
                                                                                     gctCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCg               <  1:883717/99‑1 (MQ=255)
                                                                                       tCGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCCGATCGAGGATCTCCAc             >  2:2719599/1‑101 (MQ=255)
                                                                                       tCGCCCGGCAGGGCG.C.GGC.CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGAc             <  1:1346788/101‑1 (MQ=255)
                                                                                        cGCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGAcc            <  1:2242888/101‑1 (MQ=255)
                                                                                         gCCCGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACcg           >  2:766647/1‑101 (MQ=255)
                                                                                          cccGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACcgc          <  1:3847335/101‑1 (MQ=255)
                                                                                           ccGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGGGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATTGAGGATCTCGACcgcc         >  1:1974748/1‑100 (MQ=255)
                                                                                           ccGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACcgcg         <  1:1063072/101‑1 (MQ=255)
                                                                                           ccGGCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACcgcg         >  2:2216383/1‑101 (MQ=255)
                                                                                             ggCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCCGATCGAGGATCTCCACCCCGcc       >  1:2862050/1‑101 (MQ=255)
                                                                                              gCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCg      >  2:3309728/1‑101 (MQ=255)
                                                                                              gCAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCg      <  1:1137487/101‑1 (MQ=255)
                                                                                               cAGGGCG.CGGG..CACGGGCGGTCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCgg     >  1:1123933/1‑101 (MQ=255)
                                                                                               cAGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCgg     >  2:2426388/1‑101 (MQ=255)
                                                                                                aGGGCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGc    >  2:2105852/1‑101 (MQ=255)
                                                                                                 gggCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGcc   <  2:3072151/101‑1 (MQ=255)
                                                                                                 gggCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGcc   >  2:575299/1‑101 (MQ=255)
                                                                                                  ggCG.CGGG..CACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGccc  >  1:3427553/1‑101 (MQ=255)
                                                                                                        |                                                                                                 
AAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCC.GAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGAG.TGTTCGCCCGGCAGGGCG.C.GG..CAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGCCC  >  NC_014034/3256127‑3256314

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG