Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,504,124 +C 1.6% intergenic (‑17/+66) recO ← / ← RCAP_rcc03309 DNA repair protein RecO/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,504,1241.→C 1.6% 2.5 ‑3.3 ‑0.0191intergenic (‑17/+66)recO/RCAP_rcc03309DNA repair protein RecO/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (3/0):  ref base (61/127):  total (64/127)
Fisher's exact test strand distribution p-value = 3.64e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.63e-01

Only 100 of 230 total aligned reads displayed.

CGTGGCCGTGAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTCATCCAGAAGCGTG  >  NC_014034/3504024‑3504223
                                                                                                      |                                                                                                       
cGTGGCCGTGAACGCCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.gg                                                                                                          <  2:3363509/101‑1 (MQ=255)
  tGGCCGTGAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.cc                                                                                                       >  1:17391/1‑101 (MQ=255)
   ggCCGTGAACCCCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...g                                                                                                   <  1:2044483/101‑1 (MQ=255)
   ggCCGTGAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.GC...g                                                                                                   >  2:1116791/1‑101 (MQ=255)
    gCCGTGAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...gg                                                                                                  <  2:837188/101‑1 (MQ=255)
      cGTGAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.GC...cggg                                                                                                >  1:936873/1‑101 (MQ=255)
      cGTGAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CG...gggg                                                                                                >  2:1369809/1‑101 (MQ=255)
      cGTGAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...gggg                                                                                                <  2:1520195/101‑1 (MQ=255)
       gTGAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.GC...ggggg                                                                                               >  1:2680237/1‑101 (MQ=255)
       gTGAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.GC...cgggg                                                                                               >  2:2897982/1‑101 (MQ=255)
       gTGAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...ggggg                                                                                               <  2:3494807/101‑1 (MQ=255)
          aaCACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGgtt                                                                                            >  1:1926161/1‑101 (MQ=255)
            acccctgACAATCGCCGAGCTTTCGCCGGGGAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTg                                                                                          <  1:1772828/95‑1 (MQ=255)
              ccTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGGGACGAA.GG.CC...GGGGGGTTTGCa                                                                                        >  2:709814/1‑101 (MQ=255)
                tCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGAGCCCGTGACGAA.GG.CC...GGGGGGTTTGCAcc                                                                                      >  2:4133117/1‑101 (MQ=255)
                 cGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGCGCCGCTGTCTTGCCAGTCAATGGTGCCCGTGACGAA.GG.CC...cggggggttttcccc                                                                                     >  1:616837/1‑91 (MQ=255)
                   aCAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGGTTTGCAccccc                                                                                   >  2:2792388/1‑101 (MQ=255)
                    cAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCg                                                                                  <  2:1297789/101‑1 (MQ=255)
                    cAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCg                                                                                  <  2:505390/101‑1 (MQ=255)
                      aTCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.GC...GGGGGGTTTGGACCCCCcgg                                                                                >  2:3686260/1‑101 (MQ=255)
                      aTCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCggg                                                                                <  1:1421938/101‑1 (MQ=255)
                       tCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGc                                                                               <  1:3916594/101‑1 (MQ=255)
                            gAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGGGTTGGCACCCCGGGcccccc                                                                          >  2:4024522/1‑100 (MQ=255)
                              gCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCa                                                                        <  2:3875593/101‑1 (MQ=255)
                               cTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAg                                                                       <  1:3831690/101‑1 (MQ=255)
                                tttCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGG.CCGG.GGGGGTTTTGC.CCCCCGGCcccccccg                                                                        >  1:2168037/1‑98 (MQ=255)
                                tttCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGG.CC...GGGGGTTTTTCCCCCCCGGGccccccccg                                                                       >  1:307031/1‑97 (MQ=255)
                                tttCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CCCGGGGGGGTTTTCCCCCCCCGCCCCCCCgg                                                                         >  1:2147812/1‑100 (MQ=255)
                                tttCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAgg                                                                      <  1:574435/101‑1 (MQ=255)
                                tttCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAgg                                                                      <  1:3031568/101‑1 (MQ=255)
                                tttCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAgg                                                                      <  1:2792607/101‑1 (MQ=255)
                                tttCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGGTTTGCACCCCCGGGGCCCCCCAgg                                                                      >  2:2005742/1‑101 (MQ=255)
                                tttCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGGTTTGCACCCCCCGGCCCCCGCAgg                                                                      >  2:624975/1‑101 (MQ=255)
                                tttCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...CGGGGGTTTGCACCCCCCGGccccccccgg                                                                      >  1:697409/1‑96 (MQ=255)
                                 ttCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGa                                                                     <  1:436550/101‑1 (MQ=255)
                                  tCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGat                                                                    <  1:3545870/101‑1 (MQ=255)
                                  tCGCCCTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGat                                                                    <  2:115479/101‑1 (MQ=255)
                                    gCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGatat                                                                  <  1:1018732/101‑1 (MQ=255)
                                     ccGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATAtt                                                                 <  2:1282396/101‑1 (MQ=255)
                                     ccGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATAtt                                                                 <  2:2680031/101‑1 (MQ=255)
                                     ccGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATAtt                                                                 <  1:4074324/101‑1 (MQ=255)
                                      cGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGG.CC...GGGGGGTTTGACCCCCCGGGcccccccggggactt                                                                 >  2:2327550/1‑91 (MQ=255)
                                      cGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATAttt                                                                <  1:1897336/101‑1 (MQ=255)
                                      cGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATAttt                                                                <  1:3356867/101‑1 (MQ=255)
                                      cGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATAttt                                                                <  1:2889836/101‑1 (MQ=255)
                                        tGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGa                                                              <  1:2742954/101‑1 (MQ=255)
                                         gCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAg                                                             <  1:3107969/101‑1 (MQ=255)
                                           aGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGc                                                           <  1:4197411/101‑1 (MQ=255)
                                           aGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGGTTTTGACCCCCAGGCCCCCGCCGGGTTttttatgc                                                           >  2:1115108/1‑96 (MQ=255)
                                               gggTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGGTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGa                                                       >  1:2734677/1‑101 (MQ=255)
                                                ggTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAt                                                      >  2:1739379/1‑101 (MQ=255)
                                                ggTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAt                                                      <  1:226352/101‑1 (MQ=255)
                                                 gTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.a                                                    <  1:2491224/101‑1 (MQ=255)
                                                  tCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CA...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.aa                                                   <  1:170302/101‑1 (MQ=255)
                                                    gAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.aaaa                                                 <  2:2829588/101‑1 (MQ=255)
                                                    gAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.aaaa                                                 <  1:4036696/101‑1 (MQ=255)
                                                    gAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGGTTTGCACCCCCGGGCCCCCGCAGGATATTTTAGGCAAGAT.aaaa                                                 >  2:3204277/1‑101 (MQ=255)
                                                     aaaGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CCG..GGGGGTTTTTACCCCCCGGGCCCCCCCGGGAGATTTG.GGGAAGAG.aaaaa                                                >  1:3847951/1‑100 (MQ=255)
                                                      aaGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGGTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAA.AAAAGc                                               >  1:2011075/1‑101 (MQ=255)
                                                       aGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAGCg                                              <  2:2638828/101‑1 (MQ=255)
                                                       aGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...CGGGGGTTTTCACCCCCCGGCCCCCCCCGGGTATTTTAGGGAAGAT.aaaaagg                                              >  2:3577481/1‑98 (MQ=255)
                                                          aTGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAGCGaaa                                           <  2:1733834/101‑1 (MQ=255)
                                                           tGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CCG..GGGGGGTTTTAACCCCCCGCCCCCCCCCGGGGATTTGTGGCAAAAA.aaaaagaaaa                                           >  2:1557462/1‑95 (MQ=255)
                                                            ggTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAGCGAAAGc                                         <  2:253199/101‑1 (MQ=255)
                                                             gTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAGCGAAAGcc                                        <  1:3877895/101‑1 (MQ=255)
                                                              tGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAGCGAAAGCCg                                       <  1:3923689/101‑1 (MQ=255)
                                                               gCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAGCGAAAGCCgg                                      >  2:3992311/1‑101 (MQ=255)
                                                               gCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAGCGAAAGCCgg                                      <  1:3487641/101‑1 (MQ=255)
                                                               gCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGGTTTGCACCCCCGGGCCCCCGCGGGGTATTTGGGGGAAGGT.AAAAGCGAAAGCCgg                                      >  2:3432827/1‑101 (MQ=255)
                                                                  gCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAGCGAAAGCCGGGGt                                   <  1:3686514/101‑1 (MQ=255)
                                                                   cTGTCTTGCCAGTCCATGGAGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAGCGAAAGCCGGGGTc                                  <  1:4133379/101‑1 (MQ=255)
                                                                    tGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAGCGAAAGCCGGGGTCa                                 <  1:540680/101‑1 (MQ=255)
                                                                     gTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAGCGAAAGCCGGGGTCAg                                <  2:2061667/101‑1 (MQ=255)
                                                                     gTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGGTTTTCACCCCCGGGCCCCCCCAGGATATTTGAGGGAAGAT.AAAAGCGAAAGCCGGGGGCAg                                >  2:906624/1‑101 (MQ=38)
                                                                     gTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGGTTTTCACCCCCCGGCCCCCCCCGGATATTTTAGGCAAGAA.AAAAACGAAAACCggggggag                                >  2:1032745/1‑97 (MQ=255)
                                                                        ttGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAGCGAAAGCCGGGGTCAGTCt                             >  1:1373601/1‑101 (MQ=255)
                                                                        ttGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGGTTTGCACCCCCGGGCCCCCGCAGGGTATTTGAGGCAAGAT.AAAAGCGAAAGCCGGGGTCAGTCt                             >  2:3952272/1‑101 (MQ=255)
                                                                         tGCCAGTCCATGGTGCCCGTGACGAA.GGCCC...GGGGGTTTTTACCCCCCCGGCCCCCCCGGGA.ATTTTTGGGGAGATAAAAAGGGAAACCCggggcaggctt                             >  2:139002/1‑94 (MQ=255)
                                                                         tGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGGTTTGCACCCCCCGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAACGAAAACCCGGGGCAGTCtt                            >  2:3058370/1‑101 (MQ=37)
                                                                         tGCCAGTCCATGGTGCCCGTAACGAA.CG.CG...GGGGGTTTTGAGCCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAGCGAAAGCCGGGGTCAGTCtt                            <  2:731253/101‑1 (MQ=37)
                                                                          gCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAGCGAAAGCCGGGGTCAGTCttt                           <  2:2648856/101‑1 (MQ=255)
                                                                            cAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAGCGAAAGCCGGGGTCAGTCTTTCa                         <  1:1290501/101‑1 (MQ=255)
                                                                               tCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAGCGAAAGCCGGGGTCAGTCTTTCAGTc                      <  1:2965962/101‑1 (MQ=255)
                                                                               tCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAGCGAAAGCCGGGGTCAGTCTTTCAGTc                      <  1:264586/101‑1 (MQ=255)
                                                                                gcATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAGCGAAAGCCGGGGTCAGTCTTTCAGTcc                     <  2:2363004/100‑1 (MQ=255)
                                                                                ccATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCGCCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAGCGAAAGCCGGGGTCAGTCTTTCAGTcc                     <  1:1186780/101‑1 (MQ=255)
                                                                                    ggTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCt                 <  1:379645/101‑1 (MQ=255)
                                                                                     gTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCtt                <  2:3830672/101‑1 (MQ=255)
                                                                                      tGCCCGTGACGAA.GG.CC...GGGGGTTTTGCCCCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTc               <  1:1122896/101‑1 (MQ=255)
                                                                                         ccGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTCATc            <  2:915283/101‑1 (MQ=255)
                                                                                           gTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTCATCCa          <  2:1926035/101‑1 (MQ=255)
                                                                                            tGACGAA.GG.CC...GGGGGGTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTCATCCAg         >  2:3253972/1‑101 (MQ=255)
                                                                                             gACGAA.GG.CC...GGGGGGTTTGCACCCCCGGGGCCCCCCAGGATATTTGAGGCAAGAT.AAAAACGAAAGCCGGGGGCAGTCTTTTCGTCCGTCTTCATCCCGa        >  2:1127157/1‑101 (MQ=37)
                                                                                                gAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTCATCCAGAAGc     <  1:2898201/101‑1 (MQ=255)
                                                                                                 aa.GG.CC...GGGGGTTTTTCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCAGTCTTCATCCAGAAGCg    >  2:2478780/1‑101 (MQ=255)
                                                                                                  a.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTCATCCAGAAGCGt   >  2:439847/1‑101 (MQ=255)
                                                                                                  a.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTCATCCAGAAGCGt   <  2:564382/101‑1 (MQ=255)
                                                                                                    gg.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTCATCCAGAAGCgcg  >  2:1201381/1‑99 (MQ=255)
                                                                                                    gg.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTCATCCAGAAGCGTg  <  1:1083635/101‑1 (MQ=255)
                                                                                                    gg.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTCATCCAGAAGCGTg  >  1:1043227/1‑101 (MQ=255)
                                                                                                      |                                                                                                       
CGTGGCCGTGAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAA.GG.CC...GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGAT.AAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTCATCCAGAAGCGTG  >  NC_014034/3504024‑3504223

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG