Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,701,433 +GGGCTTC 100% intergenic (+10/+39) grpE → / ← mutS GrpE protein/DNA mismatch repair protein MutS

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,701,4331.→G100.0% 108.8 40intergenic (+10/+39)grpE/mutSGrpE protein/DNA mismatch repair protein MutS
Reads supporting (aligned to +/- strand):  new base (0/40):  ref base (0/0):  total (0/40)
*NC_0140343,701,4332.→G100.0% 103.5 40intergenic (+10/+39)grpE/mutSGrpE protein/DNA mismatch repair protein MutS
Reads supporting (aligned to +/- strand):  new base (0/39):  ref base (0/0):  total (0/40)
*NC_0140343,701,4333.→G100.0% 108.2 40intergenic (+10/+39)grpE/mutSGrpE protein/DNA mismatch repair protein MutS
Reads supporting (aligned to +/- strand):  new base (0/40):  ref base (0/0):  total (0/40)
*NC_0140343,701,4334.→C100.0% 106.2 40intergenic (+10/+39)grpE/mutSGrpE protein/DNA mismatch repair protein MutS
Reads supporting (aligned to +/- strand):  new base (0/40):  ref base (0/0):  total (0/40)
*NC_0140343,701,4335.→T100.0% 108.0 40intergenic (+10/+39)grpE/mutSGrpE protein/DNA mismatch repair protein MutS
Reads supporting (aligned to +/- strand):  new base (0/40):  ref base (0/0):  total (0/40)
*NC_0140343,701,4336.→T100.0% 107.3 40intergenic (+10/+39)grpE/mutSGrpE protein/DNA mismatch repair protein MutS
Reads supporting (aligned to +/- strand):  new base (0/40):  ref base (0/0):  total (0/40)
*NC_0140343,701,4337.→C100.0% 106.8 40intergenic (+10/+39)grpE/mutSGrpE protein/DNA mismatch repair protein MutS
Reads supporting (aligned to +/- strand):  new base (0/40):  ref base (0/0):  total (0/40)

CATCATTCAGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGG.......GCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTCAGAAGGGCGCGCAGGGCGTAAAG  >  NC_014034/3701333‑3701504
                                                                                                     |||||||                                                                       
catcatTCAGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAACGCCTGAGGCAGGCCgg                                                                                <  1:3696702/101‑1 (MQ=255)
  tcatTCAGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCgg.......gg                                                                       <  1:2155682/101‑2 (MQ=255)
    atTCAGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGGCgg.......gggg                                                                     >  1:2819396/1‑98 (MQ=255)
    atTCAGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCgg.......gggc                                                                     <  2:499485/101‑4 (MQ=255)
    atTCAAGTGATGGACGACGTCTTGCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCgg.......gggc                                                                     <  2:1282283/101‑4 (MQ=255)
       cAGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCgg.......gggcttc                                                                  <  2:2773174/101‑7 (MQ=255)
       cAGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCgg.......gggcttc                                                                  <  2:1841542/101‑7 (MQ=255)
       cAGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCgg.......gggcttc                                                                  <  1:312518/101‑7 (MQ=255)
        aGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCgg.......gggcttcg                                                                 <  2:2358971/101‑8 (MQ=255)
        aGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCgg.......gggcttcg                                                                 <  2:2327365/101‑8 (MQ=255)
        aGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCgg.......gggcttcg                                                                 <  2:232205/101‑8 (MQ=255)
          gTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCgg.......gggcttcgcc                                                               <  1:3066262/101‑10 (MQ=255)
                               ctgcACGGCTGTCGGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATAtttt                                                 <  2:1176332/101‑1 (MQ=16)
                                 gcACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCa                                               <  2:2184899/101‑1 (MQ=21)
                                  cACGACCGTGTGCTACGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAg                                              <  2:905871/101‑1 (MQ=18)
                                  cACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAg                                              <  1:781147/101‑1 (MQ=21)
                                   aCGACCGGCTGCTGCGTCCGGCGAAAGGCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGc                                             <  1:1983842/101‑1 (MQ=18)
                                     gcccGTATGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCaa                                           <  2:3714847/99‑1 (MQ=18)
                                      aCCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAg                                          <  1:4143027/101‑1 (MQ=21)
                                        cGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGAt                                        <  1:2732673/101‑1 (MQ=21)
                                         gTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATa                                       <  1:1172708/101‑1 (MQ=21)
                                          tCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATaa                                      <  1:994567/101‑1 (MQ=21)
                                          tCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATaa                                      <  2:336412/101‑1 (MQ=21)
                                            tgctgcGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAg                                    <  1:466652/101‑1 (MQ=21)
                                             gctgcGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGa                                   <  1:1286261/101‑1 (MQ=21)
                                             gctgcGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGa                                   <  2:2318904/101‑1 (MQ=21)
                                              ctgcGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGAt                                  <  1:1542328/101‑1 (MQ=21)
                                              ctgcGACCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGAt                                  <  2:2188758/101‑1 (MQ=21)
                                              ctgcCTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGAt                                  <  2:768367/101‑1 (MQ=21)
                                                gcGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCa                                <  2:1312510/101‑1 (MQ=21)
                                                     cGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCtt                           <  1:1229641/101‑1 (MQ=21)
                                                     cGGCGAAAGGCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCtt                           <  1:3375290/101‑1 (MQ=21)
                                                      ggCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCttt                          <  1:1035683/101‑1 (MQ=21)
                                                       gCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTc                         <  2:448966/101‑1 (MQ=21)
                                                        cGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTCa                        <  2:4098895/101‑1 (MQ=21)
                                                        cGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTCa                        <  1:2829600/101‑1 (MQ=21)
                                                          aaaGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGTGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTCAGa                      <  2:2301888/101‑1 (MQ=25)
                                                            aGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTCAGAAg                    <  1:2964383/101‑1 (MQ=21)
                                                             gTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTCAGAAgg                   <  1:2126676/101‑1 (MQ=21)
                                                              tCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTCAGAAggg                  <  1:2285207/101‑1 (MQ=21)
                                                              tCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTCAGAAggg                  <  2:4070571/101‑1 (MQ=21)
                                                               cGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTCAGAAGGgc                 <  1:2027165/101‑1 (MQ=21)
                                                                 gCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTCAGAAGGgcgc               <  2:3328001/101‑1 (MQ=21)
                                                                  cGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTCAGAAGGgcgcg              <  2:3231677/101‑1 (MQ=21)
                                                                     gAGTTCGATGCGGAAAGCCTGAGGCTGGCCGGGAGCTTCGCGCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTCAGAAGGGCGCGCAg           <  2:107296/101‑1 (MQ=19)
                                                                     gAGTTCGATGCCGAAAGCCTGAGGCGGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTCAGAAGGGCGCGCAg           <  2:1019829/101‑1 (MQ=21)
                                                                      aGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTCAGAAGGGCGCGCAgg          <  1:3722447/101‑1 (MQ=21)
                                                                       ggtCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTCAGAAGGGCGCGCAggg         <  1:1019045/99‑1 (MQ=21)
                                                                        ttCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGCCCCCCAGGATATTTTCAGAAAGATAAAGATCAGTCTTTCAGAAGGGCGCGCAGGGc        <  2:2605639/101‑1 (MQ=21)
                                                                        ttCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTCAGAAGGGCGCGCAGGGc        <  1:2199852/101‑1 (MQ=21)
                                                                          cGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTCAGAAGGGCGCGCAGGGCGt      <  1:919667/101‑1 (MQ=21)
                                                                           gATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTCAGAAGGGCGCGCAGGGCGTa     <  2:3025946/101‑1 (MQ=21)
                                                                            aTGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTCAGAAGGGCGCGCAGGGCGTaa    <  2:1755229/101‑1 (MQ=21)
                                                                              gCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTCAGAAGGGCGCGCAGGGCGTAAAg  <  2:2192825/101‑1 (MQ=21)
                                                                                                     |||||||                                                                       
CATCATTCAGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGG.......GCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTCAGAAGGGCGCGCAGGGCGTAAAG  >  NC_014034/3701333‑3701504

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG