BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	586,917	+G	100%	intergenic (‑145/+37)	RCAP_rcc00548 ← / ← purL	BolA family protein/phosphoribosylformylglycinamidine synthase II

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	586,917	1	.→G	100.0%	22.1	125	intergenic (‑145/+37)	RCAP_rcc00548/purL	BolA family protein/phosphoribosylformylglycinamidine synthase II
Reads supporting (aligned to +/- strand): new base (70/46): ref base (0/9): total (70/55)
Fisher's exact test strand distribution p-value = 4.16e-04
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.09e-01

Only 100 of 154 total aligned reads displayed.


GGCGACAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTGCCAGCG  >  NC_014034/586817‑587012


 gCGAGAAGAGCCAAGCGCCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.g                                                                                                <  1:4210912/101‑1 (MQ=255)


  cGACAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.gg                                                                                               <  2:1071802/101‑1 (MQ=255)


  cGACAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.gg                                                                                               <  1:2727877/101‑1 (MQ=255)


  cGACAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.gg                                                                                               >  2:3635987/1‑101 (MQ=255)


  cGACAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.gg                                                                                               >  1:2667228/1‑101 (MQ=255)


    aCAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.gggg                                                                                             >  1:2343508/1‑101 (MQ=255)


      aaGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCGGCGGGCAGGGGCCGCGGGGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.gggggg                                                                                           <  2:1430999/101‑1 (MQ=255)


      aaGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.gggggg                                                                                           <  1:937718/101‑1 (MQ=255)


      aaGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.gggggg                                                                                           >  2:3973098/1‑101 (MQ=255)


        gagCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.gggggggg                                                                                         >  1:783080/1‑100 (MQ=255)


        gagCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.gggggggg                                                                                         >  2:3431570/1‑100 (MQ=255)


             aaGGACCGGTTGCGTGTGCCAAGTGACGCCGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCtt                                                                                     <  1:2514345/101‑1 (MQ=255)


             aaGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCtt                                                                                     >  1:3660192/1‑101 (MQ=255)


             aaGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCtt                                                                                     >  2:1580520/1‑101 (MQ=255)


             aaGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCtt                                                                                     >  2:2490703/1‑101 (MQ=255)


              aGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTc                                                                                    >  1:3892315/1‑101 (MQ=255)


               gCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCt                                                                                   >  2:759454/1‑101 (MQ=255)


               gCACCGGTTGCGGGGGCCAAGGGGCGCAGGGGGCGGGGGCCGGGGCGAGGACGGGGGTCAGGGCGGGGGCAAGTGGCGCCGAGC.T.CGGGGGGGGCGCTTCt                                                                                   <  1:798903/101‑1 (MQ=255)


                cACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTg                                                                                  >  1:1725393/1‑101 (MQ=255)


                 aCCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCtcc                                                                                 >  1:1092825/1‑99 (MQ=255)


                   cGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTgccc                                                                               >  2:2175146/1‑101 (MQ=255)


                    ggTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTgcccg                                                                              >  1:1973779/1‑101 (MQ=255)


                     gTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTgcccgc                                                                             >  2:2611101/1‑101 (MQ=255)


                          gtgtGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCgg                                                                        >  1:3417857/1‑101 (MQ=255)


                          gtgtGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCgg                                                                        >  2:4015894/1‑101 (MQ=255)


                           tgtgCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGa                                                                       >  1:300651/1‑101 (MQ=255)


                              gCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCt                                                                    >  1:2848645/1‑101 (MQ=255)


                               ccAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCtt                                                                   >  2:2644822/1‑101 (MQ=255)


                               ccAAGGGACGGGGGGGGCGGGGGCCGGGGGGGGGACGGGGGTCGGGACGGGGGCAAGGGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCtt                                                                   <  2:3710854/101‑1 (MQ=255)


                                 gagggACGCGGCGGGCAGGGGCCGCGGGGGGGGCGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGc                                                                 <  1:3445377/97‑1 (MQ=255)


                                 gagggACGCAGGGGGCGGGGGCCGGGGGGGGGGCGGGGGTGGGGAGGGGGGAAAGTGGCGCCGAGC.TGG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGc                                                                 <  2:1477300/97‑1 (MQ=255)


                                   ggggcgcggggggCGGGGGCCGCGGGGGGGGGGGGGGGCAGGACGGGGGCAAGTGCCGCCGGGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGt                                                               <  1:3729044/91‑1 (MQ=255)


                                   gTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGt                                                               <  2:300606/101‑1 (MQ=255)


                                      gcgcggggggGAGGGGCCGGGGGGAGGGCGGGGGTCGGGAGGGGGGCAAGTGTCGCCGAGC.TGG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGtttt                                                            <  1:116289/94‑1 (MQ=255)


                                       gggggggggCGGGGGCCGCGGGGGGGGGGGGGGGCAGGAGGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTc                                                           <  1:4080125/95‑1 (MQ=255)


                                        gcggggggggggggCGGGGGCGAGGGGGGGGGTCGGGACGGGGGCAAGTGTCGCCGGGCGG.G.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCa                                                          <  1:3300802/91‑1 (MQ=255)


                                        gCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCa                                                          <  2:4093182/101‑1 (MQ=255)


                                         cAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAg                                                         >  1:3493859/1‑101 (MQ=255)


                                           gggggCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGcc                                                       <  1:644270/99‑1 (MQ=255)


                                            cGGGCAGGGGCCGCGGCGAGGACGGTGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCt                                                      >  1:258480/1‑101 (MQ=255)


                                            cGGGCAGGGGCCGCGGCGAGGACGGGGGTCGGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCt                                                      <  2:2848426/101‑1 (MQ=255)


                                            cGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCt                                                      >  2:1641726/1‑101 (MQ=255)


                                            cGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCt                                                      >  1:208851/1‑101 (MQ=255)


                                             gggCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCtt                                                     >  1:2754691/1‑101 (MQ=255)


                                             gggCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCtt                                                     <  2:701503/101‑1 (MQ=255)


                                             gggCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCtt                                                     >  1:3377123/1‑101 (MQ=255)


                                              gggaGGGGCCGCGGCGAGGACGGGGGGCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTc                                                    <  2:1697508/98‑1 (MQ=255)


                                               gcgggggGCGGGGGGAGGACGGGGGTCAGGGGGGGGGCAAGTGTCGCCGGGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCg                                                   <  2:877049/98‑1 (MQ=255)


                                               gcgggggCCGCGGCGAGGAGGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCg                                                   <  1:158523/98‑1 (MQ=255)


                                               gCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCg                                                   <  2:3892056/101‑1 (MQ=255)


                                                cAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGGGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGGTTTCAGCCTTCGa                                                  >  1:1756589/1‑101 (MQ=255)


                                                 aGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGGGCTTCTGCCCGCCCCGGAGCCTGCGTTTTCAGCCTTCGAc                                                 >  1:379642/1‑101 (MQ=255)


                                                 aGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAc                                                 >  1:3032294/1‑101 (MQ=255)


                                                 aGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAc                                                 >  1:4080514/1‑101 (MQ=255)


                                                 aGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAc                                                 >  2:520338/1‑101 (MQ=255)


                                                  ggggGCGCGGCGAGGAGGGGGGTTGGGGCGGGGGAAAGTCTCCCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAcg                                                <  2:198612/101‑1 (MQ=255)


                                                  ggggCCGGGGCGGGGGGGGGGGTCAGGCCGGGGGCAAGGGGCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAcg                                                <  1:3973358/101‑1 (MQ=255)


                                                  ggggCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAcg                                                >  2:2867379/1‑101 (MQ=255)


                                                   gggCCGGGGCGGGGGGGGGGGTCAGGACGGGGGCAAGGGCCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAcgg                                               <  1:2969256/101‑1 (MQ=255)


                                                    ggCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAcggc                                              <  1:677142/101‑1 (MQ=255)


                                                       cgcgGCGAGGGGGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAcggcggc                                           <  2:1808815/101‑1 (MQ=255)


                                                       cgcgGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAcggcggc                                           >  1:1234467/1‑101 (MQ=255)


                                                         cggcgAGGAGGGGGGGCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAcggcggcgg                                         <  2:89624/101‑1 (MQ=255)


                                                           gggAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGgcggcggcg                                       >  1:3496122/3‑101 (MQ=255)


                                                              aGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGCAg                                    >  2:3227240/1‑101 (MQ=255)


                                                                  ggggggGCGGGAGGGGGGCAAGTGTCGCCGAGC.TGG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgcgg                                <  1:98542/100‑1 (MQ=255)


                                                                   gggggTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgcggt                               >  1:2039995/1‑101 (MQ=255)


                                                                    ggggTCAGGGCGGGGGCAGGTGTCGCCGAGC.TGG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgcggtg                              <  1:3509912/101‑1 (MQ=255)


                                                                    ggggTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgcggtg                              >  2:114642/1‑101 (MQ=255)


                                                                      ggTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgcggtgcg                            <  2:1620292/101‑1 (MQ=255)


                                                                        tCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgcggtgcggt                          <  2:2511495/101‑1 (MQ=255)


                                                                        tCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgcggtgcggt                          <  2:3376878/101‑1 (MQ=255)


                                                                        gaggggcGGGGGCAGGGGTCGCGGAGC.TGG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgcggtgcggt                          <  2:2200536/95‑1 (MQ=255)


                                                                         cAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTa                         >  2:2731613/1‑101 (MQ=255)


                                                                          ggggcGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTaa                        <  1:2720615/97‑1 (MQ=255)


                                                                           ggACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTaaa                       <  2:1379411/101‑1 (MQ=255)


                                                                             aCGGGGGCAAGTGTCGCCGAGC.C.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGc                     <  2:1214048/101‑1 (MQ=255)


                                                                              cGGGGGCAGGTGTGGCCGGGC.T.CGGGGGGGGCCCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCg                    <  1:1580629/101‑1 (MQ=255)


                                                                               gggggCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGTCTTCGACGGCGGCGGCGAAGGCGGGGCGGTAAAGCGt                   >  1:3571370/1‑101 (MQ=255)


                                                                               gggggCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGt                   >  2:1892579/1‑101 (MQ=255)


                                                                               gggggCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGt                   >  1:2751944/1‑101 (MQ=255)


                                                                               gggggCAAGTGTCGCCGAGC.T.CGGGGGGGGAGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGGGCGGTAAGGCGt                   >  2:3530514/1‑101 (MQ=255)


                                                                                ggggCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTg                  >  1:339204/1‑101 (MQ=255)


                                                                                ggggCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTg                  <  1:3087717/101‑1 (MQ=255)


                                                                                 gggCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGc                 <  1:398814/101‑1 (MQ=255)


                                                                                  ggCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCt                <  2:2771582/101‑1 (MQ=255)


                                                                                   gCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTg               <  2:696413/101‑1 (MQ=255)


                                                                                   gCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTg               <  1:2645028/101‑1 (MQ=255)


                                                                                     aaGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAg             <  1:2994992/101‑1 (MQ=255)


                                                                                      aGTGTCGCCGAGC.TGG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGc            <  2:522842/101‑1 (MQ=255)


                                                                                      aGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGc            >  1:1273451/1‑101 (MQ=255)


                                                                                         gtCGCCGGGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGctct         <  1:3530767/101‑1 (MQ=255)


                                                                                          tCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGCAGGCGGTGCGGTAAAGCGTGCTGAGCTCTg        >  1:1993333/1‑101 (MQ=255)


                                                                                          tCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTg        <  1:2441824/101‑1 (MQ=255)


                                                                                           cggcGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTGc       <  2:3130935/98‑1 (MQ=255)


                                                                                             cggAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTGCCa     <  1:2611292/99‑1 (MQ=255)


                                                                                             ccGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGGGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTGCCa     >  1:2867623/1‑101 (MQ=255)


                                                                                              cGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTGCCAg    >  2:3005430/1‑101 (MQ=255)


                                                                                               gAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTGCCAgc   <  2:2787384/101‑1 (MQ=255)


                                                                                                aGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTCTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTGCCAgcg  >  1:2813042/1‑101 (MQ=255)


GGCGACAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTGCCAGCG  >  NC_014034/586817‑587012

Base quality scores:


ATCG

< 3 ≤


ATCG

< 14 ≤


ATCG

< 23 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG