Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	900,742	+C	100%	intergenic (‑71/‑216)	ldh ← / → ftsW	L‑lactate dehydrogenase/cell division protein FtsW

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	900,742	1	.→C	100.0%	303.6	86	intergenic (‑71/‑216)	ldh/ftsW	L‑lactate dehydrogenase/cell division protein FtsW
Reads supporting (aligned to +/- strand):  new base (36/50):  ref base (0/0):  total (36/50)

CCCCGCGCCCACGATTCCGACCTTCATCGGCCGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCG.CGCG.G.CGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GC...CGCGGAG..CCCGCGGGTCGCCCCGGCCCTGCCGGGGCG  >  NC_014034/900645‑900832                                                                                                     |                                                                                                   ccccGCGCCCACGATTCCGACCTTCATCGCCCGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCgcg.g.cc                                                                                                  <  1:1710506/101‑2 (MQ=255) ccccGCGCCCACGATTCCGACCTTCATCGCCCGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCgcg.g.cc                                                                                                  <  1:1900147/101‑2 (MQ=255)  cccGCGCCCACGATTCCGACCTTCATCGCCCGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCgcg.g.ccg                                                                                                 <  2:2530827/101‑3 (MQ=255)     gcgcCCACGATTCCGACCTTCATCGCCCGTCTCCCGTTTGTGATGGCGCTACCACGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCgcg.g.ccgccg                                                                                              <  2:3658319/101‑6 (MQ=255)       gcCCACGATTCCGACCTTCATCGCCCGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCt                                                                                             >  2:599644/1‑101 (MQ=255)        cccACGATTCCGACCTTCATCGCCCGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCtt                                                                                            <  2:4196392/101‑1 (MQ=255)        cccACGATTCCGACCTTCATCGCCCGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCtt                                                                                            <  2:1219209/101‑1 (MQ=255)         ccACGATTCCGACCTTCATCGCCCGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCttt                                                                                           <  1:325515/101‑1 (MQ=255)         ccACGATTCCGACCTTCATCGCCCGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCttt                                                                                           <  1:880954/101‑1 (MQ=255)         ccACGATTCCGACCTTCATCGCCCGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCttt                                                                                           <  1:1359799/101‑1 (MQ=255)         ccACGATTCCGACCTTCATCGCCCGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCttt                                                                                           <  1:956897/101‑1 (MQ=255)         ccACGATTCCGACCTTCATCGCCCGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCttt                                                                                           <  1:2077005/101‑1 (MQ=255)         ccACGATTCCGACCTTCATCGCCCGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCttt                                                                                           <  2:2221791/101‑1 (MQ=255)          cACGATTCCGACCTTCATCGCCCGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTg                                                                                          <  1:3393674/101‑1 (MQ=255)          cACGATTCCGACCTTCATCGCCCGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTg                                                                                          <  2:215826/101‑1 (MQ=255)           ccGATTCCGACCTTCATCGCCCGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGc                                                                                         <  1:122318/100‑1 (MQ=255)           aCGATTCCGACCTTCATCGCCCGTCTCCCGTTTGTGATGGCGCTGCCATGGCACGCTCCCGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGc                                                                                         <  1:2061384/101‑1 (MQ=255)           aCGATTCCGACCTTCATCGCCCGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGc                                                                                         <  1:4013188/101‑1 (MQ=255)             gATTCCGACCTTCATCGCCCGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCg                                                                                       >  2:1874284/1‑101 (MQ=255)             gATTCCGACCTTCATCGCCCGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCg                                                                                       >  2:3894540/1‑101 (MQ=255)             gATTCCGACCTTCATCGCCCGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCg                                                                                       >  2:409596/1‑101 (MQ=255)                 ccGACCTTCATCGCCCGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGc                                                                                   >  2:3189103/1‑101 (MQ=255)                  cGACCTTCATCGCCCGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGcc                                                                                  <  1:1622250/101‑1 (MQ=255)                   gACCTTCATCGCCCGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGccc                                                                                 >  2:2151495/1‑101 (MQ=255)                      cTTCATCGCCCGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCgg                                                                              <  2:1557687/101‑1 (MQ=255)                      cTTCATCGCCCGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCgg                                                                              <  1:2709654/101‑1 (MQ=255)                      cTTCATCGCCCGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCgg                                                                              >  1:574584/1‑101 (MQ=255)                        tCATCGCCCGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGtt                                                                            <  2:3440834/101‑1 (MQ=255)                          aTCGCCCGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGtttt                                                                          <  1:239453/101‑1 (MQ=255)                           tCGCCCGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTc                                                                         <  2:887366/101‑1 (MQ=255)                             gcccGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCt                                                                       <  2:1564574/99‑1 (MQ=255)                             gcccGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCt                                                                       <  2:4159778/99‑1 (MQ=255)                              cccGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCtt                                                                      <  1:292762/100‑1 (MQ=255)                              cccGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCtt                                                                      >  1:1632397/2‑101 (MQ=255)                               ccGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCGCC.CGCCGCTTTGCCGCAGCCCCGGTTTTCCTTg                                                                     <  1:4228412/101‑1 (MQ=255)                               ccGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTg                                                                     <  1:3039215/101‑1 (MQ=255)                               ccGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTg                                                                     >  2:848457/1‑101 (MQ=255)                                cGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCATgg                                                                    <  1:3257738/101‑1 (MQ=255)                                 gTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGt                                                                   >  1:1519532/1‑101 (MQ=255)                                   ctcCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGc                                                                 >  1:1121603/1‑101 (MQ=255)                                    tcCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCa                                                                >  1:1089313/1‑101 (MQ=255)                                    tcCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCa                                                                >  1:1801884/1‑101 (MQ=255)                                    tcCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCa                                                                <  1:253301/101‑1 (MQ=255)                                      ccGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCaaa                                                              <  1:3143600/101‑1 (MQ=255)                                      ccGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCaaa                                                              <  1:2962252/101‑1 (MQ=255)                                       cGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAAt                                                             <  2:3346792/101‑1 (MQ=255)                                       cGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAAt                                                             <  1:409647/101‑1 (MQ=255)                                         tttGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATAc                                                           <  2:459565/101‑1 (MQ=255)                                         tttGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATAc                                                           <  1:2965620/101‑1 (MQ=255)                                          ttGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACg                                                          <  1:3894799/101‑1 (MQ=255)                                           tgttATGCCCCTACCATGGCCCGCTCACGCGTCCTCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGc                                                         <  2:235152/101‑1 (MQ=255)                                           tgtgATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGc                                                         >  2:2399080/1‑101 (MQ=255)                                           tgtgATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGc                                                         <  2:1865518/101‑1 (MQ=255)                                           tgtgATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGc                                                         >  2:2930806/1‑101 (MQ=255)                                            gtgATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGcc                                                        <  2:3919836/101‑1 (MQ=255)                                             tgATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGccc                                                       <  1:936736/101‑1 (MQ=255)                                             tgATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGccc                                                       <  1:1780612/101‑1 (MQ=255)                                              gATGGCGCTAGCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCg                                                      <  2:2057396/101‑1 (MQ=255)                                               aTGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCgg                                                     <  1:1828206/101‑1 (MQ=255)                                                tGGCGCTACCATGGCGCGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCggg                                                    <  2:1193936/101‑1 (MQ=255)                                                tGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCggg                                                    >  1:313050/1‑101 (MQ=255)                                                  gcgcTACCATGGGACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCggggg                                                  >  1:2299775/1‑101 (MQ=255)                                                  gcgcTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCggggg                                                  <  2:1984221/101‑1 (MQ=255)                                                    gcTGCCATGGCACGCTCCCCCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...g                                             <  1:599708/101‑1 (MQ=255)                                                    gcTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...g                                             >  2:182964/1‑101 (MQ=255)                                                     cTACCATGGCCCGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...Gc                                            <  1:2723692/101‑1 (MQ=255)                                                     cTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...Gc                                            >  1:1108603/1‑101 (MQ=255)                                                     cTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...Gc                                            <  1:1054706/101‑1 (MQ=255)                                                     cTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...Gc                                            <  1:3189342/101‑1 (MQ=255)                                                       aCCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...Gcgg                                          >  1:36013/1‑99 (MQ=255)                                                       aCCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...Gcgg                                          <  1:619731/101‑3 (MQ=255)                                                       aCCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...Gcgg                                          >  2:3716614/1‑99 (MQ=255)                                                        ccATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...Gcggg                                         <  2:317546/101‑4 (MQ=255)                                                        ccATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...Gcggg                                         <  1:939850/101‑4 (MQ=255)                                                         cATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...Gcgggg                                        >  1:725960/1‑97 (MQ=38)                                                          aTGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...Gcggggg                                       >  1:204905/1‑96 (MQ=38)                                                            ggCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...Gcgggggcg                                     >  1:1431509/1‑94 (MQ=38)                                                             gCACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...Gcgggggcgg                                    <  2:3306738/101‑9 (MQ=38)                                                              cACGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...Gcgggggcgga                                   <  2:3217951/101‑10 (MQ=38)                                                               aCGCTCACGCGTCCGCGCAACCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...Gcgggggcggag                                  >  1:378449/1‑91 (MQ=37)                                                                                 aaCCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGAGCCCCCGCGGGTCGCCCCg                >  2:1563191/1‑101 (MQ=16)                                                                                 aaCCCCGTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGAGCCCCCGCGGGTCGCCCCg                >  2:2131464/1‑101 (MQ=16)                                                                                      cGTTCCCGCCGCG.GCCGCCGCTTTGCCGCGGCCCCGGTTTTCCTTGGTGCAAATCCGCCGGGGGGTGGGGG...GGCGGAGCCCCCGCGGGTCGCCCCGGCCCt           <  1:3835906/101‑1 (MQ=9)                                                                                       gTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGTAGCCCCCGCGGGTCCCCCCGGCCCTg          >  2:730911/1‑101 (MQ=16)                                                                                       gTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGAGCCCCCGCGGGTCGCCCCGGCCCtc          >  1:3244644/1‑100 (MQ=16)                                                                                       gTTTCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGAGCCCCCGCGGGTCGCCCCGGCCCTg          >  2:2239609/1‑101 (MQ=16)                                                                                         ttCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGAGCCCCCGCGGGTCGCCCCGGCCCTGcc        >  2:886575/1‑101 (MQ=16)                                                                                         ttCCGCCGCG.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGAGCCCCCGCGGGTCGCCCCGGCCCTGcc        >  2:3382714/1‑101 (MQ=16)                                                                                         tcccgccgcg.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGAGCCCCCGCGGGTCGCCCCGGCCCTGcc        <  2:603174/95‑1 (MQ=16)                                                                                            cgccgcg.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGAGCCCCCGCGGGTCGCCCCGGCCCTGCCggg     >  1:2003301/4‑101 (MQ=18)                                                                                             gccgcg.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGAGCCCCCGCGGGTCGCCCCGGCCCTGCCgggg    >  2:603206/3‑101 (MQ=18)                                                                                             gccgcg.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGAGCCCCCGCGGGTCGCCCCGGCCCTGCCgggg    >  2:4055007/3‑101 (MQ=18)                                                                                              ccgcg.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGAGCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGc   >  2:2298178/2‑101 (MQ=18)                                                                                               cgcg.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGAGCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCg  >  1:366158/1‑101 (MQ=18)                                                                                               cgcg.GCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GCGGGGGCGGAGCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCg  >  2:2759122/1‑101 (MQ=18)                                                                                                     |                                                                                                   CCCCGCGCCCACGATTCCGACCTTCATCGGCCGTCTCCCGTTTGTGATGGCGCTACCATGGCACGCTCACGCGTCCGCGCAACCCCGTTTCCG.CGCG.G.CGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCCCGGGGGT...GC...CGCGGAG..CCCGCGGGTCGCCCCGGCCCTGCCGGGGCG  >  NC_014034/900645‑900832 Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG