Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 925,909 +C 94.1% intergenic (+162/+12) RCAP_rcc00838 → / ← glcB DnaJ domain‑containing protein/malate synthase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034925,9091.→C94.1% 22.3 ‑0.3 ‑1.4168intergenic (+162/+12)RCAP_rcc00838/glcBDnaJ domain‑containing protein/malate synthase
Reads supporting (aligned to +/- strand):  new base (95/63):  ref base (7/3):  total (102/66)
Fisher's exact test strand distribution p-value = 7.42e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.23e-02

Only 100 of 186 total aligned reads displayed.

ACCCCCCGGGATATTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CG.CCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATCCGGGCGAGGAA  >  NC_014034/925809‑926004
                                                                                                        |                                                                                               
cccccccGGGATATTTGGGGCAAGGTGAAAGCAGGCCGCTGTGCCCTTTCATCTGGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAg.cg                                                                                                  <  2:3061689/100‑1 (MQ=255)
  cccccGGGATATTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CG.cc                                                                                               <  2:2689928/101‑1 (MQ=255)
   ccccGGGATATTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CG.ccc                                                                                              >  1:947074/1‑101 (MQ=255)
    cccGGGATATTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CG.cccc                                                                                             <  2:3758667/101‑1 (MQ=255)
      cGGGATATTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CG.cccccc                                                                                           <  2:3978787/101‑1 (MQ=255)
      cGGGATATTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CG.cccccc                                                                                           >  1:1928794/1‑101 (MQ=255)
      cGGGATATTTAGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTCGCGGACGGGGGGCGG.CG.cccccc                                                                                           >  2:735820/1‑101 (MQ=255)
       gggATATTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CG.ccccccc                                                                                          >  2:3726928/1‑101 (MQ=255)
       gggATATTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CG.ccccccc                                                                                          >  2:1346525/1‑101 (MQ=255)
        ggATATTGGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CG.cccccccc                                                                                         <  2:4230469/101‑2 (MQ=255)
         gATTTTTGGGGCAAGGGGAAAGCAGGCCGTTTTGCCCTTTCATCTTTGCCCAAA.TATCCCGGGGGGA.GCCCGCTTGCGGACGGGGGGCAG.CG.cccccccct                                                                                        <  1:562576/101‑3 (MQ=21)
          atatTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGGAG.CG.CCCCCCCctt                                                                                       >  1:410722/1‑101 (MQ=255)
          atatTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CG.CCCCCCCctt                                                                                       >  2:889311/1‑101 (MQ=255)
          atatTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CG.CCCCCCCctt                                                                                       >  2:1758991/1‑101 (MQ=255)
          atatTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CG.CCCCCCCctt                                                                                       >  1:903730/1‑101 (MQ=255)
            atTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CG.CCCCCCCctttc                                                                                     >  1:1233745/1‑99 (MQ=255)
             tttGGGGCGAGGTGAAAGCAGGCCGCGTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCt                                                                                     <  2:1980893/101‑1 (MQ=18)
              ttGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCtt                                                                                    >  1:612266/1‑101 (MQ=255)
                ggggCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTAt                                                                                  <  1:3323861/101‑1 (MQ=255)
                 gggCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATg                                                                                 <  1:1974209/101‑1 (MQ=255)
                 gggCAAGGTGAAAACAGGCCGCTTTGCCCTTTCATCTTGCCCAAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATg                                                                                 <  2:18692/101‑1 (MQ=21)
                  ggCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGc                                                                                >  2:3654144/1‑101 (MQ=255)
                    cAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCtt                                                                              >  1:2549533/1‑101 (MQ=255)
                    cAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCtt                                                                              <  1:996759/101‑1 (MQ=255)
                    cAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCtt                                                                              >  2:4085718/1‑101 (MQ=255)
                     aaGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCttt                                                                             >  2:1628430/1‑101 (MQ=255)
                     aaGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCttt                                                                             >  2:3336202/1‑101 (MQ=255)
                     aaGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCttt                                                                             >  2:3426815/1‑101 (MQ=255)
                      aGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTg                                                                            <  2:4132104/101‑1 (MQ=255)
                       ggTGAACCCCGGCCCCTTCCCCCTTTCCCCCTCCCCCAAACTCCCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTg                                                                            <  2:3636912/101‑1 (MQ=2)
                         tGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTggcg                                                                         >  1:1799113/1‑101 (MQ=255)
                         tGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTggcg                                                                         >  1:1177462/1‑101 (MQ=255)
                          gAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAA.CGCCCCCCCCTTTCTTATGCTTTggcgg                                                                        >  1:1551743/1‑101 (MQ=255)
                           aaaGCAGGCCGCTTTGCCCTTTCTTCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTggcggc                                                                       <  2:3853664/101‑1 (MQ=255)
                            aaGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGcc                                                                      >  2:470521/1‑101 (MQ=255)
                            aaGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGACAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGcc                                                                      >  1:3604326/1‑101 (MQ=255)
                               cAGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTc                                                                   <  1:3953463/101‑1 (MQ=255)
                               cAGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTc                                                                   <  2:624490/101‑1 (MQ=255)
                                aGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTcc                                                                  >  1:1840668/1‑100 (MQ=255)
                                 ggCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCAc                                                                 <  1:1678659/101‑1 (MQ=255)
                                  gCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCAcc                                                                <  1:2650347/101‑1 (MQ=255)
                                   ccGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCt                                                               <  2:3209972/101‑1 (MQ=255)
                                   ccGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCt                                                               >  1:1765583/1‑101 (MQ=255)
                                   ccGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCt                                                               >  2:2976692/1‑101 (MQ=255)
                                    cGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTg                                                              <  2:2709518/101‑1 (MQ=255)
                                    cGCTTTGCCCTTTCATCTTGCCCAAAA.TACCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTg                                                              <  1:2144398/101‑1 (MQ=21)
                                     gCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTgc                                                             <  2:1350697/101‑1 (MQ=255)
                                      cTTTGCCCTTTCCTCTTGCCCCAAA.TATCCGGGGGGGA.GTCCTCTTGCGGACGGGGGGAAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTgcg                                                            <  1:2684180/101‑1 (MQ=25)
                                        ttGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTAGCGACCTTCACCTGCGcc                                                          >  1:3655846/1‑101 (MQ=21)
                                           cccTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCgg                                                       <  1:2392530/101‑1 (MQ=255)
                                             cTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGgcg                                                     >  1:3934903/1‑101 (MQ=255)
                                              tttCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGgcgc                                                    >  1:2216772/1‑101 (MQ=255)
                                                  agcTTGCCCAAAA.TA.CCGGGGGGGAGGTCCGGTTGCGGAGGGGGGGAAGCCC.CCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGt                                                <  1:2379922/99‑1 (MQ=255)
                                                  aTCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTggg                                                >  2:2214217/1‑100 (MQ=255)
                                                  aTCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGt                                                >  2:839343/1‑101 (MQ=255)
                                                     ttGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTgca                                             <  2:2410070/101‑1 (MQ=255)
                                                        ccccAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTgcagca                                          >  1:817870/1‑101 (MQ=255)
                                                         cccAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCAc                                         <  2:2547309/101‑1 (MQ=255)
                                                         cccAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCAc                                         >  1:3973571/1‑101 (MQ=255)
                                                            aaa.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGGGCCCGGCGCTCGTGCCGCACCgg                                      >  2:3785078/1‑101 (MQ=21)
                                                             aa.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCCCCTGCGCCCGGCGCTGGGgcagccaccgc                                     >  1:3405564/1‑95 (MQ=21)
                                                              a.tatCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCt                                    >  2:2360701/1‑101 (MQ=255)
                                                                tatCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTc                                   <  1:2966796/101‑1 (MQ=255)
                                                                tatCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTc                                   <  1:514106/101‑1 (MQ=255)
                                                                    ccGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGta                               >  2:525686/1‑101 (MQ=255)
                                                                    ccGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGta                               <  2:2416408/101‑1 (MQ=255)
                                                                     cGGGGGTA.GTCCGCTTGCGGACGGGGGCCCGCCC.CCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGtat                              <  1:1346625/101‑1 (MQ=37)
                                                                     cGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTccggat                              >  1:3368038/1‑96 (MQ=255)
                                                                     cGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGtat                              <  2:675562/101‑1 (MQ=255)
                                                                      ggggggA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGtata                             <  1:2818941/101‑1 (MQ=255)
                                                                       gggggA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAg                            <  2:2382280/101‑1 (MQ=255)
                                                                       gggggA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAg                            >  2:2319302/1‑101 (MQ=255)
                                                                       gggggA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAg                            >  2:2025128/1‑101 (MQ=255)
                                                                        ggggA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGc                           >  2:2118450/1‑101 (MQ=255)
                                                                         gggA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGcc                          <  1:3834082/101‑1 (MQ=255)
                                                                         gggA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGcc                          >  1:682538/1‑101 (MQ=255)
                                                                         gggA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGcc                          >  2:1053934/1‑101 (MQ=255)
                                                                          ggA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGccc                         >  1:4148727/1‑101 (MQ=255)
                                                                          ggA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGccc                         <  1:2070387/101‑1 (MQ=255)
                                                                          ggA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGccc                         <  2:1407169/101‑1 (MQ=255)
                                                                          cgA.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCCCCCGGCGCTGGTGCAGCACCGGCTCTGGATAGgcc                         >  2:1173623/2‑101 (MQ=25)
                                                                            a.GTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGGATAGCCCGa                       >  2:2482304/1‑101 (MQ=255)
                                                                             ggTCCGCTGGCGGGCGGGGGCCCGCCC.CCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGa                       <  2:1840548/100‑1 (MQ=255)
                                                                              gTCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAg                      >  1:312782/1‑101 (MQ=255)
                                                                               tCCGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAgg                     <  2:2629037/101‑1 (MQ=255)
                                                                                ccgatctcGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGa                    <  2:585956/94‑1 (MQ=38)
                                                                                ccGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTTACCTGCGCCCGGCGCTGGTGCAGAACCGGCTCGGTATAAACCCAGGa                    >  2:3641288/1‑101 (MQ=25)
                                                                                 cGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGAc                   >  2:4146323/1‑101 (MQ=255)
                                                                                 cGCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGAc                   <  2:3580223/101‑1 (MQ=255)
                                                                                  gCTTGCGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACt                  <  1:2714437/101‑1 (MQ=255)
                                                                                       cGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGaagatcgg             >  1:586020/1‑94 (MQ=38)
                                                                                       cGGACGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACGGGGTc             >  1:770862/1‑101 (MQ=38)
                                                                                         gccGGGGGGCGC.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATCCg           <  1:3042780/99‑1 (MQ=255)
                                                                                           cGGGGGGCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATCCggg         <  2:4029599/101‑1 (MQ=255)
                                                                                            ggggggCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCCGGATAGGCCGAGGGCTGGATCCgggg        >  2:2442668/1‑100 (MQ=255)
                                                                                             gggggCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATCCGGGCg       >  1:4026496/1‑101 (MQ=255)
                                                                                             gggggCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATCCGGGCg       <  1:2323699/101‑1 (MQ=255)
                                                                                              ggggCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATCCGGGCGa      <  1:986693/101‑1 (MQ=255)
                                                                                                ggCAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCCAGGACCGGATCCGGGCGCgg    >  1:111555/1‑101 (MQ=255)
                                                                                                  cAG.CGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATCCGGGCGAGGaa  >  2:4768/1‑101 (MQ=255)
                                                                                                        |                                                                                               
ACCCCCCGGGATATTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAA.TATCCCGGGGGGA.GTCCGCTTGCGGACGGGGGGCAG.CG.CCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATCCGGGCGAGGAA  >  NC_014034/925809‑926004

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG