Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,224,5850G→A 1.7% 1.9 0.0 ‑0.0228T144T (ACC→ACThisA1‑(5‑phosphoribosyl)‑5‑[(5‑ phosphoribosylamino)methylideneamino] imidazole‑4‑carboxamide isomerase
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (114/110):  total (116/112)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.90e-01

Only 100 of 236 total aligned reads displayed.

TCGGGCCGGTCATCGCGCCGTCGCGCAGGATGTCGGTGTAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCGGGAAGGCCTTCGCCGCTTCGCGTACGAGAT  >  NC_014034/1224485‑1224685
                                                                                                    |                                                                                                    
  gggCCGGTCATCGCGCCGTCGCGCAGGATGTCGGTGTAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGt                                                                                                    <  2:2137821/101‑1 (MQ=255)
  gggCCGGTCATCGCGCCGTCGCGCAGGATGTCGGTGTAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGt                                                                                                    <  1:3285084/101‑1 (MQ=255)
   ggCCGGTCATCGCGCCGTCGCGCAGGATGTCGGTGTAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGtt                                                                                                   <  2:1493129/101‑1 (MQ=255)
    gCCGGTCATCGCGCCGTCGCGCAGGATGTCGGTGTAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGttt                                                                                                  <  2:1242167/101‑1 (MQ=255)
     ccGGTCATCGCGCCGTCGCGCAGGATGTCGGTGTAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTc                                                                                                 <  2:1913387/101‑1 (MQ=255)
       ggTCATCGCGCAGTCGCGCAGGATGTCGGTGTAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCg                                                                                               >  2:3753301/1‑101 (MQ=255)
         tCATCGCGCCGTCGCGCAGGATGTCGGTGTAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTg                                                                                             >  1:227433/1‑101 (MQ=255)
             cgcgCCGTCGCGCAGGATGTCGGTGTAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGccc                                                                                         <  1:1554689/101‑1 (MQ=255)
             cgcgCCGTCGCGCAGGATGTCGGTGTAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCAGTTTCCGTGGccc                                                                                         >  2:2312097/1‑101 (MQ=255)
               cgcCGTCGCGCAGGATGTCGGTGTAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAg                                                                                       <  1:841017/101‑1 (MQ=255)
                gcCGTCGCGCAGGATGTCGGTGTAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGc                                                                                      >  1:4188608/1‑101 (MQ=255)
                gcCGTCGCGCAGGATGTCGGTGTAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGc                                                                                      >  2:3065232/1‑101 (MQ=255)
                gcCGTCGCGCAGGATGTCGGTGGAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGc                                                                                      <  1:947015/101‑1 (MQ=255)
                   gTCGCGCAGGATGTCGGTGTAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCt                                                                                   >  2:272589/1‑101 (MQ=255)
                   gTCGCGCAGGATGTCGGTGTAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCt                                                                                   >  2:4221339/1‑101 (MQ=255)
                    tCGCGCAGGATGTCGGTGTAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCtt                                                                                  <  2:1030610/101‑1 (MQ=255)
                       cgcAGGATGTCGGTGTAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGt                                                                               >  1:3140989/1‑101 (MQ=255)
                        gcAGGATGTCGGTGTAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTc                                                                              <  2:3759629/101‑1 (MQ=255)
                         cAGGATGTCGGTGTAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCg                                                                             <  2:663642/101‑1 (MQ=255)
                         cAGGATGTCGGTGTAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCg                                                                             >  2:3157332/1‑101 (MQ=255)
                         cAGGATGTCGGTGTAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCg                                                                             >  1:2671456/1‑101 (MQ=255)
                            gATGTCGGTGTAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCa                                                                          >  2:725718/1‑101 (MQ=255)
                               gTCGGTGTAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCt                                                                       <  1:2431701/101‑1 (MQ=255)
                               gTCGGTGTAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCt                                                                       >  1:3199197/1‑101 (MQ=255)
                                tCGGTGTAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCtt                                                                      <  2:3273443/101‑1 (MQ=255)
                                 cGGTGTAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTg                                                                     <  1:1224403/101‑1 (MQ=255)
                                 cGGTGTAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTg                                                                     >  2:3027210/1‑101 (MQ=255)
                                 cGGTGTAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTg                                                                     >  2:2660260/1‑101 (MQ=255)
                                 atctgtAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTg                                                                     <  2:4193432/98‑1 (MQ=255)
                                    tgtAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCg                                                                  <  1:452932/101‑1 (MQ=255)
                                     gtAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGt                                                                 >  1:935659/1‑101 (MQ=255)
                                     gtAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGt                                                                 >  1:1651045/1‑101 (MQ=255)
                                      tAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGtt                                                                <  2:362272/101‑1 (MQ=255)
                                       aGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGTGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTc                                                               <  1:3570511/101‑1 (MQ=255)
                                       aGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTc                                                               >  2:3777826/1‑101 (MQ=255)
                                       aGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTc                                                               >  1:1915632/1‑101 (MQ=255)
                                          tgatCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTc                                                            >  1:2073772/1‑101 (MQ=255)
                                             tCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCa                                                         <  1:1826233/101‑1 (MQ=255)
                                             tCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCa                                                         >  2:3411415/1‑101 (MQ=255)
                                               gccgccACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATc                                                       >  1:2287171/1‑101 (MQ=255)
                                               gccgccACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCAAGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACNTTGCCGTTCCTCGCNTc                                                       >  2:819395/1‑101 (MQ=255)
                                                ccgccACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCg                                                      >  1:4232325/1‑101 (MQ=255)
                                                ccgccACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCg                                                      <  2:1364859/101‑1 (MQ=255)
                                                 cgccACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGa                                                     <  1:3873851/101‑1 (MQ=255)
                                                 cgccACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGa                                                     >  2:2991127/1‑101 (MQ=255)
                                                 cgccACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGa                                                     <  1:2566385/101‑1 (MQ=255)
                                                  gccACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGAt                                                    <  2:431709/101‑1 (MQ=255)
                                                   ccACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCAGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATg                                                   <  1:2312265/101‑1 (MQ=255)
                                                   ccACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCAGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATg                                                   <  1:484452/101‑1 (MQ=255)
                                                    cACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGc                                                  <  2:2280605/101‑1 (MQ=255)
                                                      gagggctggcTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCg                                                >  2:3583977/10‑101 (MQ=255)
                                                       gCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCTTGGCCCAGCCCCTGGTCGCCACCTTGCCGTTCCTCGCATCGGTGCCGa                                               >  2:557901/1‑101 (MQ=255)
                                                        cccGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGAc                                              >  2:1878372/1‑101 (MQ=255)
                                                        cccACATCTTCGAAGCCTTGCGCCAGATCCGTCCCCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCGCCTTGCCGTTCCTCGCATCGATGCCGAc                                              <  2:476370/101‑1 (MQ=255)
                                                         ccGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGAcc                                             <  2:112854/101‑1 (MQ=255)
                                                            cATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGcc                                          <  1:2591869/101‑1 (MQ=255)
                                                             aTCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCa                                         >  1:3709143/1‑101 (MQ=255)
                                                              tCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCAc                                        >  1:3522088/1‑101 (MQ=255)
                                                                ttCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCTACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACtt                                      >  1:2946467/1‑101 (MQ=255)
                                                                ttCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACtt                                      >  1:2805553/1‑101 (MQ=255)
                                                                ttCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACtt                                      >  1:1334534/1‑101 (MQ=255)
                                                                 tCGAAGCTTTGCGCCAGATCCGCCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTg                                     >  1:4098283/1‑101 (MQ=255)
                                                                  cGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGc                                    >  1:698070/1‑101 (MQ=255)
                                                                   gAAGCTTTGCGCCCGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGcc                                   <  1:2819579/101‑1 (MQ=255)
                                                                   gAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGcc                                   >  1:2175614/1‑101 (MQ=255)
                                                                    aaGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGccc                                  <  2:1718347/101‑1 (MQ=255)
                                                                     aGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCCCTTGcccc                                 >  2:864431/1‑101 (MQ=255)
                                                                      gCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCg                                <  2:233097/101‑1 (MQ=255)
                                                                       cTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCgg                               <  1:772265/101‑1 (MQ=255)
                                                                        tttGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCggg                              <  2:3986661/101‑1 (MQ=255)
                                                                        tttGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCggg                              <  1:1529611/101‑1 (MQ=255)
                                                                          tGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCGGGaa                            >  1:4016464/1‑101 (MQ=255)
                                                                           gcgcCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCGGGAAg                           <  1:3065457/101‑1 (MQ=255)
                                                                            cgcCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCGGGAAgg                          <  2:2029372/101‑1 (MQ=255)
                                                                            cgcCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCGGGAAgg                          >  2:2944661/1‑101 (MQ=255)
                                                                            cgcCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCGGGAAgg                          <  2:3848223/101‑1 (MQ=255)
                                                                             gcCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCGGGAAGGc                         >  1:2085526/1‑101 (MQ=255)
                                                                               cAGATCCGTCACCAGCACGTCGGTTTACGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCGGGAAGGCCt                       <  1:2545968/101‑1 (MQ=255)
                                                                                aGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCGGGAAGGCCtt                      <  1:1459914/101‑1 (MQ=255)
                                                                                 gATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCTCCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCGGGAAGGCCTTc                     <  2:1956888/101‑1 (MQ=255)
                                                                                 gATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCGGGAAGGCCTTc                     <  1:2135583/101‑1 (MQ=255)
                                                                                 gATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCGGGAAGGCCTTc                     >  2:167772/1‑101 (MQ=255)
                                                                                  aTCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCGGGAAGGCCTTcg                    >  1:1876147/1‑101 (MQ=255)
                                                                                  aTCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCGGGAAGGCCTTcg                    <  1:67015/101‑1 (MQ=255)
                                                                                  aTCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCGGGAAGGCCTTcg                    >  1:2994020/1‑101 (MQ=255)
                                                                                  aTCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCGGGAAGGCCTTcg                    <  2:41059/101‑1 (MQ=255)
                                                                                    ccGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCGGGAAGGCCTTcgcc                  <  2:2863947/101‑1 (MQ=255)
                                                                                        cACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCGGGAAGGCCTTCGCCGCtt              <  1:621236/101‑1 (MQ=255)
                                                                                         aCCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCGGGAAGGCCTTCGCCGCTTc             <  1:3593958/101‑1 (MQ=255)
                                                                                          ccAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCGGGAAGGCCTTCGCCGCTTcg            <  2:1624877/101‑1 (MQ=255)
                                                                                           cAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCGGGAAGGCCTTCGCCGCTTcgc           <  2:1534860/101‑1 (MQ=255)
                                                                                           cAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCGGGAAGGCCTTCGCCGCTTcgc           >  2:489051/1‑101 (MQ=255)
                                                                                           cAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCGGGAAGGCCTTCGCCGCTTcgc           <  1:2351771/101‑1 (MQ=255)
                                                                                                   cGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCGGGAAGGCCTTCGCCGCTTCGCGTACgaga   >  1:1067380/1‑101 (MQ=255)
                                                                                                   cGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCGGGAAGGCCTTCGCCGCTTCGCGTACgaga   <  1:3302153/101‑1 (MQ=255)
                                                                                                   cGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCGGGAAGGCCTTCGCCGCTTCGCGTACgaga   <  2:324237/101‑1 (MQ=255)
                                                                                                   cGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCGGGAAGGCCTTCGCCGCTTCGCGTACgaga   >  2:1850222/1‑101 (MQ=255)
                                                                                                    ggTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCGGGAAGGCCTTCGCCGCTTCGCGTACGAGAt  >  1:2880905/1‑101 (MQ=255)
                                                                                                    ggTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCGGGAAGGCCTTCGCCGCTTCGCGTACGAGAt  <  1:3380862/101‑1 (MQ=255)
                                                                                                    ggTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCGGGAAGGCCTTCGCCGCTTCGCGTACGAGAt  >  2:2560434/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
TCGGGCCGGTCATCGCGCCGTCGCGCAGGATGTCGGTGTAGATGATCGCCGCCACGCCCGCATCTTCGAAGCTTTGCGCCAGATCCGTCACCAGCACGTCGGTTTCCGTGGCCCAGCCCTTGGTCGCCACCTTGCCGTTCCTCGCATCGATGCCGACCGCCACTTGCCCCGGGAAGGCCTTCGCCGCTTCGCGTACGAGAT  >  NC_014034/1224485‑1224685

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG