Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,5451.→A15.6% 150.4 ‑1.9 ‑2.2231coding (427/1578 nt)RCAP_rcc01307hypothetical protein
Rejected: Prediction has significantly lower quality scores supporting polymorphism compared to reference.
Reads supporting (aligned to +/- strand):  new base (14/22):  ref base (104/91):  total (118/113)
Fisher's exact test strand distribution p-value = 1.46e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.27e-03

Only 100 of 250 total aligned reads displayed.

CCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTC  >  NC_014034/1396445‑1396645
                                                                                                     |                                                                                                     
cccccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTc                                                                                                        <  2:1889584/101‑1 (MQ=255)
 ccccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.g                                                                                                      <  2:1219158/101‑1 (MQ=255)
  cccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.Gt                                                                                                     <  1:2011050/101‑1 (MQ=255)
    cgcgGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTgg                                                                                                   >  1:1403750/1‑101 (MQ=255)
      cgGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGGAACGCGGCCGCGAGCACGTCGCTCCCCCCGCGGGGGGGTGGAACGAAACCGCTTTC.GTGGGa                                                                                                 >  2:899959/1‑101 (MQ=38)
        ggggCGTGGATCGAAACGGACCTGAAATTCCTCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACt                                                                                               <  2:2361109/101‑1 (MQ=255)
         gggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTg                                                                                              <  2:1149564/101‑1 (MQ=255)
             gTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCa                                                                                          >  2:1095347/1‑101 (MQ=255)
              tGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAg                                                                                         >  2:3669357/1‑101 (MQ=255)
               ggATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgc                                                                                        >  1:2849742/1‑101 (MQ=255)
               ggATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgc                                                                                        >  2:3730966/1‑101 (MQ=255)
                   cGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGGCAgccgcc                                                                                    >  1:587087/1‑101 (MQ=255)
                    gAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGGCGCTCCCCCCGCGGGGGCGAGGACCGAAACTGCTTTC.GTGGGGCTGGCTAGccgccc                                                                                   >  1:3613982/1‑101 (MQ=38)
                      aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgccc.gc                                                                                >  1:3060254/1‑101 (MQ=255)
                      aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgccc.gc                                                                                <  2:2567817/101‑1 (MQ=255)
                       aCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCc.gcg                                                                               >  1:2879154/1‑101 (MQ=255)
                           aCCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCaa                                                                           <  2:3131673/101‑1 (MQ=255)
                             cTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGt                                                                         >  2:2519203/1‑101 (MQ=255)
                             cTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGt                                                                         >  1:2112474/1‑101 (MQ=255)
                                aaaTTCCGCGGGAAACGCGGTGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGc                                                                      >  1:1104376/1‑101 (MQ=255)
                                aaaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGc                                                                      <  2:4062228/101‑1 (MQ=255)
                                  aTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTc                                                                    >  1:1764399/1‑101 (MQ=255)
                                   ttCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCAGCGccgacctcggc                                                                    <  1:789723/101‑10 (MQ=37)
                                   ttCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTcc                                                                   >  2:363685/1‑101 (MQ=255)
                                    tCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTccc                                                                  >  1:2366114/1‑101 (MQ=255)
                                        cgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCCCTccccccc                                                              >  2:3151363/1‑100 (MQ=255)
                                         gggAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCcgc                                                             >  2:3115237/1‑101 (MQ=255)
                                         gggAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCcgc                                                             >  2:2027019/1‑101 (MQ=255)
                                          ggAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTGCCCCcgcg                                                            >  2:3206959/1‑101 (MQ=255)
                                              aCGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCCAGTCGCTCCCCCCGCggggg                                                        >  1:1679214/1‑101 (MQ=255)
                                               cgcgGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGc                                                       <  1:1892748/101‑1 (MQ=255)
                                               cgcgGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGc                                                       <  1:127694/101‑1 (MQ=255)
                                               cgcgGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCCCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGc                                                       >  2:1664402/1‑101 (MQ=255)
                                                 cggcggcgAGCACGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGt                                                     <  2:595327/101‑1 (MQ=255)
                                                   gcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTgg                                                   <  1:2562820/101‑1 (MQ=255)
                                                   gcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTgg                                                   >  2:3904755/1‑101 (MQ=255)
                                                    cggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGa                                                  <  1:3500837/101‑1 (MQ=255)
                                                    cggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGa                                                  <  2:1408839/101‑1 (MQ=255)
                                                    cggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGa                                                  >  1:1921959/1‑101 (MQ=255)
                                                     tgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGa                                                  <  1:3481420/92‑1 (MQ=2)
                                                     tcttccgatctCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                 <  1:3107294/91‑1 (MQ=255)
                                                     gcctcgCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCTGCTTTC.GTGGGACTCGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                 <  2:433268/96‑1 (MQ=255)
                                                      gcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                <  1:3373078/101‑1 (MQ=255)
                                                       ggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATc                                                >  1:710791/8‑101 (MQ=2)
                                                        gacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCg                                               <  1:63788/95‑1 (MQ=2)
                                                        gacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCg                                               <  2:777493/95‑1 (MQ=2)
                                                         acggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGa                                              <  1:776596/96‑1 (MQ=2)
                                                         acggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGa                                              <  2:1998846/96‑1 (MQ=2)
                                                         acggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGa                                              >  2:1872176/6‑101 (MQ=2)
                                                          gCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                            <  2:1518126/101‑1 (MQ=255)
                                                           cACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                           <  1:3176931/101‑1 (MQ=255)
                                                           cACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                           >  1:2625086/1‑101 (MQ=255)
                                                             ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAAct                                          <  2:1034304/100‑2 (MQ=2)
                                                             cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGTCT.CCGGGCAGTCGCCTCCCCCGCCGGGGCGCGGTTCGAAAAGa                                         >  2:3791587/1‑101 (MQ=18)
                                                             agTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGGGGATCGAAAct                                          >  2:1708598/2‑100 (MQ=2)
                                                              ggcGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                        <  2:2652742/99‑1 (MQ=255)
                                                              gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGagatcggaag                                         >  1:46186/1‑92 (MQ=2)
                                                              gTCGCTCCCACCGCGGGGGCGCGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCCCCC.GCGCCAAGTCGCTCCCCCCCCGTGGGCGTGGATCGACACGac                                        >  2:477429/1‑100 (MQ=38)
                                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAttt                                      <  2:936574/101‑1 (MQ=255)
                                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAttt                                      <  2:131230/101‑1 (MQ=255)
                                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                                     <  1:152770/101‑1 (MQ=255)
                                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                                     <  2:3966768/101‑1 (MQ=255)
                                                                  cTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcg                                    <  1:2764271/101‑1 (MQ=255)
                                                                  cTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGCACTGGCCAGCCCCCC.GCGCCAAGTCGCTCCCCCCGCCGGGGCGCCGATCGAAACGATTtcg                                    >  1:973087/1‑101 (MQ=37)
                                                                   cccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgt                                   <  2:3257949/100‑1 (MQ=255)
                                                                    ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                  <  1:93156/101‑1 (MQ=255)
                                                                    ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                  >  2:4121428/1‑101 (MQ=255)
                                                                     cccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtcg                                 >  2:156160/1‑101 (MQ=255)
                                                                     cccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggt                                  >  2:3922592/1‑92 (MQ=2)
                                                                     cccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggt                                  >  2:3029435/1‑92 (MQ=2)
                                                                       cccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCt                               <  1:4121689/101‑1 (MQ=255)
                                                                        ccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCtt                              <  1:509974/101‑1 (MQ=255)
                                                                        ccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCtt                              >  2:509971/1‑101 (MQ=255)
                                                                        ccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCtt                              <  1:1475602/101‑1 (MQ=255)
                                                                         cgcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTa                             >  1:964675/1‑101 (MQ=255)
                                                                          gcgGGGGTGTGGATCGAAACTGCTTTC.GTGGGAGTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                            <  1:3665318/101‑1 (MQ=255)
                                                                          gcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                            <  2:4207517/101‑1 (MQ=255)
                                                                           cgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacg                           <  2:3991475/101‑1 (MQ=255)
                                                                            gggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacga                          <  2:3592805/101‑1 (MQ=255)
                                                                             ggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgac                         >  2:3861487/1‑101 (MQ=255)
                                                                             ggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgac                         >  1:1037435/1‑101 (MQ=255)
                                                                                gCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGcc                      <  1:1607830/101‑1 (MQ=255)
                                                                                 cGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCt                     >  2:3434577/1‑101 (MQ=255)
                                                                                   tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                   <  1:2250318/101‑1 (MQ=255)
                                                                                   tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                   >  2:2866427/1‑101 (MQ=255)
                                                                                    ggATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATc                  >  1:11561/1‑101 (MQ=255)
                                                                                    ggATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATc                  >  2:2229406/1‑101 (MQ=255)
                                                                                    ggATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATAGAAACGATTTAGCCGCTTACGACGCCTATc                  >  1:3810044/1‑101 (MQ=255)
                                                                                     gATTGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCt                 <  1:2123366/101‑1 (MQ=255)
                                                                                      aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg                >  1:3083165/1‑101 (MQ=255)
                                                                                        cGAAACTGCTTTC.GTGGGACTGGCCAGCAGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAt              >  2:3056537/1‑101 (MQ=255)
                                                                                         gAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAtt             >  1:4056389/1‑101 (MQ=255)
                                                                                         gAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAtt             <  2:2386246/101‑1 (MQ=255)
                                                                                          aaaCTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAttt            >  1:3828199/1‑101 (MQ=255)
                                                                                          aaaCTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAttt            <  1:1966419/101‑1 (MQ=255)
                                                                                               gCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGt       >  1:2461570/1‑101 (MQ=255)
                                                                                               gCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGt       <  1:871722/101‑1 (MQ=255)
                                                                                                  ttC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGc    >  1:3270673/1‑101 (MQ=255)
                                                                                                   tC.GGGGGACTGGCCGGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCt   <  2:2197882/101‑1 (MQ=255)
                                                                                                    c.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTc  >  1:432826/1‑101 (MQ=255)
                                                                                                     |                                                                                                     
CCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTC  >  NC_014034/1396445‑1396645

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG