BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,273,716	G→T	3.3%	G1021G (GGC→GGA)	pyc ←	pyruvate carboxylase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,273,716	0	G→T	3.3%	4.8 ‑4.2 ‑0.0	199	G1021G (GGC→GGA)	pyc	pyruvate carboxylase
Reads supporting (aligned to +/- strand): new base (7/0): ref base (95/95): total (103/96)
Fisher's exact test strand distribution p-value = 1.44e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.97e-01

Only 100 of 218 total aligned reads displayed.


CCCGCACCGCGCGCGGCTGGCCGTTGAGTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGACCGGGGTGGGCAGCGTCCGCACCGGGCCATAGACCCGATGCCGCC  >  NC_014034/1273616‑1273816


cccGCACCGCGCGCGGCTGGCCGTTGAGTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTg                                                                                                      <  1:1465909/101‑1 (MQ=255)


cccGCACCGCGCGCGGCTGGCCGTTGAGTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTg                                                                                                      <  1:634609/101‑1 (MQ=255)


 ccGCACCGCGCGCGGCTGGCCGTTGAGTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGc                                                                                                     >  1:4204781/1‑101 (MQ=255)


   gCACCGCGCGCGGCTGGCCGTTGAGTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGccc                                                                                                   >  2:1082302/1‑101 (MQ=255)


    cACCGCGCGCGGCTGGCCGTTGAGTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCg                                                                                                  >  2:2155547/1‑101 (MQ=255)


    cACCGCGCGCGGCTGGCCGTTGAGTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCg                                                                                                  <  1:776930/101‑1 (MQ=255)


     cccGCGCGCGGCTGGCCGTTGAGTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCgg                                                                                                 <  1:230777/100‑1 (MQ=255)


     aCCGCGCGCGGCTGGCCGTTGAGTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGCCTTCTCCgg                                                                                                 >  1:2874375/1‑101 (MQ=255)


        gcgcgcgGCTGGCCGTTGAGTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCggggc                                                                                              >  1:3435403/1‑99 (MQ=255)


          gcgcgGCTGGCCGTTGAGTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGa                                                                                            >  1:4029514/1‑101 (MQ=255)


          gcgcgGCTGGCCGTTGAGTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGa                                                                                            >  1:1933097/1‑101 (MQ=255)


           cgcgGCTGGCCGTTGAGTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGAt                                                                                           >  2:510342/1‑101 (MQ=255)


           cgcgGCTGGCCGTTGAGTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGAt                                                                                           <  1:2138879/101‑1 (MQ=255)


           cgcgGCTGGCCGTTGAGTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCCGGGCGAt                                                                                           >  2:2977403/1‑101 (MQ=255)


             cgGCTGGCCGTTGAGTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGAtct                                                                                         >  2:4226333/1‑101 (MQ=255)


             cgGCTGGCCGTTGAGTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGAtct                                                                                         >  2:2349731/1‑101 (MQ=255)


              ggCTGGCCGTTGAGTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGAtctc                                                                                        <  1:4007847/101‑1 (MQ=255)


              ggCTGGCCGTTGAGTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGAtctc                                                                                        <  1:3170657/101‑1 (MQ=255)


               gCTGGCCGTTGAGTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGCTCTCg                                                                                       >  1:2996027/1‑101 (MQ=255)


                cTGGCCGTTGAGTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCgg                                                                                      >  1:1702410/1‑101 (MQ=255)


                 tGGCCGTTGAGTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGc                                                                                     >  1:3951866/1‑101 (MQ=255)


                 tGGCCGTTGAGTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGc                                                                                     <  2:491663/101‑1 (MQ=255)


                 tGGCCGTTGAGTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCAGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGc                                                                                     >  1:3604461/1‑101 (MQ=255)


                    ccGTTGAGTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCga                                                                                  >  2:3123950/1‑101 (MQ=255)


                      gTTGAGTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCgaga                                                                                >  2:2190266/1‑101 (MQ=255)


                      gTTGAGTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCgaga                                                                                >  2:769746/1‑101 (MQ=255)


                      gTTGAGTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGGCGATCTCGGCCgaga                                                                                >  2:1139783/1‑101 (MQ=255)


                       ttGAGTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTTCCCGGGTCGATCTCGGCCGAGAt                                                                               >  1:61799/1‑101 (MQ=255)


                         gAGTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCTCGGGTCGATCTCGGCCGAGAttt                                                                             <  2:2041022/101‑1 (MQ=255)


                         gAGTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGAgtt                                                                             >  2:1858213/1‑101 (MQ=255)


                          aGTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTc                                                                            <  2:565554/101‑1 (MQ=255)


                          aGTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTc                                                                            <  1:720482/101‑1 (MQ=255)


                           gTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTTTTCGGGGCGCTCTCCGCCgagcgtttc                                                                           >  1:1986784/1‑95 (MQ=255)


                           gTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTcc                                                                           <  1:2503242/101‑1 (MQ=255)


                            ttCGAAGAAGGCCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCGCGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCg                                                                          <  1:4057658/101‑1 (MQ=255)


                            ttCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGGCGATCTCGGCCGAGATTTTCg                                                                          >  1:3274193/1‑101 (MQ=255)


                              cGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAg                                                                        <  1:238307/101‑1 (MQ=255)


                                aagaagACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGGCGATCTCGGCCGAGATTTTCGAGcc                                                                      >  2:3646825/1‑101 (MQ=255)


                                  gaagcCCTTGGCATCGCCGGTCCCGTCGGTCTCGCCCGCGGTCTGCAGCCGGATCACCACCGTCTTGCCCGGGTCGATCTTGGCCGAGATTTCCGAGCCgg                                                                    <  2:1383882/101‑1 (MQ=255)


                                   aagaCCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCggg                                                                   >  2:1986652/1‑101 (MQ=255)


                                    ggaCCTTGGCATCGCCGGTCTGGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGgc                                                                  <  2:2665287/100‑1 (MQ=255)


                                     gaCCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGgcg                                                                 >  1:1073689/1‑101 (MQ=255)


                                     gaCCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGgcg                                                                 >  1:3246298/1‑101 (MQ=255)


                                       ccTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGcc                                                               <  1:1139877/101‑1 (MQ=255)


                                       ccTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGcc                                                               >  1:1033651/1‑101 (MQ=255)


                                        cTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCa                                                              <  1:1832743/101‑1 (MQ=255)


                                         ttGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCAt                                                             >  1:2557201/1‑101 (MQ=255)


                                         ttGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCAt                                                             >  1:474237/1‑101 (MQ=255)


                                         ttGGCATCGCCGGTCTCGTCGGTCTCGCCCACCGTCTGCAGCCGGATGTCCAGCGTCTTACCCGGGCCGGTCTGGACCGGGAGTTCCGCGCCGGgcgcgcg                                                             >  2:3703575/1‑98 (MQ=255)


                                          tGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATg                                                            <  2:1689615/101‑1 (MQ=255)


                                          tGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATg                                                            >  2:3603323/1‑101 (MQ=255)


                                          tGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATg                                                            <  2:123610/101‑1 (MQ=255)


                                           ggCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGc                                                           >  1:26537/1‑101 (MQ=255)


                                            gCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGGCGGGCGCCaatcc                                                          >  2:3266407/1‑97 (MQ=255)


                                             cATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCg                                                         <  2:3951606/101‑1 (MQ=255)


                                                cGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTag                                                      <  2:4000966/101‑1 (MQ=255)


                                                 gCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTaga                                                     <  1:1582421/101‑1 (MQ=255)


                                                   cGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTagaag                                                   >  1:4114834/1‑101 (MQ=255)


                                                   cGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTagaag                                                   <  1:1292867/101‑1 (MQ=255)


                                                    ggTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTagaaga                                                  >  2:1440392/1‑101 (MQ=255)


                                                     gTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTagaagaa                                                 <  2:1932971/101‑1 (MQ=255)


                                                      gctcGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTagaagaag                                                <  2:1947847/100‑1 (MQ=255)


                                                        tcgtcgGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGAc                                              <  1:1347441/101‑1 (MQ=255)


                                                         cgtcgGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGAcc                                             >  2:3164468/1‑101 (MQ=255)


                                                         cgtcgGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGAcc                                             >  2:4150176/1‑101 (MQ=255)


                                                         cgtcgGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGAcc                                             >  1:3899117/1‑101 (MQ=255)


                                                            cgGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGACCggg                                          <  1:3101583/101‑1 (MQ=255)


                                                             ggTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGACCgggg                                         >  2:2419966/1‑101 (MQ=255)


                                                             ggTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGACCgggg                                         >  1:979564/1‑101 (MQ=255)


                                                             ggTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGACCgggg                                         <  2:2860465/101‑1 (MQ=255)


                                                              gTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGACCGGGGt                                        <  2:2698929/101‑1 (MQ=255)


                                                                ctcGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGACCGGGGTgg                                      >  2:3445833/1‑101 (MQ=255)


                                                                ctcGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGACCGGGGGgg                                      >  1:1743338/1‑101 (MQ=255)


                                                                 tcGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGACCGGGGTggg                                     <  2:2598586/101‑1 (MQ=255)


                                                                 tcGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGACCCGGGTggg                                     >  2:3776256/1‑101 (MQ=255)


                                                                       aCGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGACCGGGGTGGGCAGCGt                               >  2:3282249/1‑101 (MQ=255)


                                                                         ggTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGACCGGGGTGGGCAGCGTcc                             >  1:2985029/1‑101 (MQ=255)


                                                                         ggTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGACCGGGGTGGGCAGCGTcc                             <  1:3721892/101‑1 (MQ=255)


                                                                         ggTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGACCGGGGGGGGCAGCGTcc                             >  2:1625807/1‑101 (MQ=255)


                                                                           tCTGCAGCCGGATTTCCAGCGTCTTTCCCCGGGCGGTCTCGGCCCAGATTTCCCGGCCCGGCGCCCCGCCGTAGAAAAAGACCGGGGGGGGGAGCGGCCGc                           >  2:3488647/1‑101 (MQ=255)


                                                                           tCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGACCGGGGTGGGCAGCGTCCGc                           <  2:3210198/101‑1 (MQ=255)


                                                                            cTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGACCGGGGTGGGCAGCGTCCGCa                          >  2:3372024/1‑101 (MQ=255)


                                                                               cAGCCGGATTTCCAGCGTCTTTCCCCGGGCGGTCTCGGCCGGGATTTCCGAGCCGGGGGCCCTGCCGTAGAAGAAGACCCGGGGGGGGAGCGGCCGCACCg                       >  2:2532052/1‑101 (MQ=255)


                                                                                aGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGACCGGGGTGGGCAGCGTCCGCACCgg                      >  2:648996/1‑101 (MQ=255)


                                                                                   cGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGACCGGGGTGGGCAGCGTCCGCACCGGGcc                   <  2:457960/101‑1 (MQ=255)


                                                                                    ggATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGACCGGGGTGGGCAGCGTCCGCACCGGGCCa                  <  1:2125046/101‑1 (MQ=255)


                                                                                    ggATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGACCGGGGTGGGCAGCGTCCGCACCGGGCCa                  >  2:2737502/1‑101 (MQ=255)


                                                                                    ggATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGACCGGGGTGGGCAGCGTCCGCACCGGGCCa                  <  2:2175119/101‑1 (MQ=255)


                                                                                     gATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGACCGGGGTGGGCAGCGTCCGCACCGGGCCAt                 <  1:205215/101‑1 (MQ=255)


                                                                                     gATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGACCGGGGTGGGCAGCGTCCGCACCGGGCCAt                 <  2:1881058/101‑1 (MQ=255)


                                                                                        ttCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGACCGGGGGGGGCAGCGTCCGCACCGGGCCCTAGa              >  1:2792508/1‑101 (MQ=255)


                                                                                         tCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGACCGGGGTGGGCAGCGTCCGCACCGGGCCATAGAc             >  2:1650238/1‑101 (MQ=255)


                                                                                          ccAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGACCGGGGTGGGCAGCGTCCGCACCGGGCCATAGAcc            >  1:974227/1‑101 (MQ=255)


                                                                                           cAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGACCGGGGTGGGCAGCGTCCGCACCGGGCCATAGAccc           <  2:4204519/101‑1 (MQ=255)


                                                                                            aGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGACCGGGGTGGGCAGCGTCCGCACCGGGCCATAGACCCg          <  2:4029253/101‑1 (MQ=255)


                                                                                             gCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGACCGGGGTGGGCAGCGTCCGCACCGGGCCATAGACCCGa         <  1:2441143/101‑1 (MQ=255)


                                                                                              cGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGACCGGGGTGGGCAGCGTCCGCACCGGGCCATAGACCCGAt        <  1:2714386/101‑1 (MQ=255)


                                                                                                tCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGACCGGGGTGGGCAGCGTCCGCACCGGGCCATAGCCCCGATgc      >  1:3500269/1‑101 (MQ=255)


                                                                                                  ttGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGACCGGGGTGGGCAGCGTCCGCACCGGGCCATAGACCCGATgccg    <  1:4226595/101‑1 (MQ=255)


                                                                                                    gCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGACCGGGGTGGGCAGCGTCCGCACCGGGCCATAGACCCGATgccgcc  >  2:1005882/1‑101 (MQ=255)


CCCGCACCGCGCGCGGCTGGCCGTTGAGTTCGAAGAAGACCTTGGCATCGCCGGTCTCGTCGGTCTCGCCCACGGTCTGCAGCCGGATTTCCAGCGTCTTGCCCGGGTCGATCTCGGCCGAGATTTCCGAGCCGGGCGCCATGCCGTAGAAGAAGACCGGGGTGGGCAGCGTCCGCACCGGGCCATAGACCCGATGCCGCC  >  NC_014034/1273616‑1273816

Base quality scores:


ATCG

< 3 ≤


ATCG

< 19 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 41 ≤


ATCG