Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,388,183 A→T 100% M246F (ATC→TTC)  RCAP_rcc01300 → Cas3 family CRISPR‑associated helicase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,388,1830A→T100.0% 730.7 184M246F (ATC→TTC) RCAP_rcc01300Cas3 family CRISPR‑associated helicase
Reads supporting (aligned to +/- strand):  new base (95/89):  ref base (0/0):  total (95/89)

Only 100 of 186 total aligned reads displayed.

CGCATATGGCCGCCTTTGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGATCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACGGGCATCGGCGGATCATCTA  >  NC_014034/1388085‑1388280
                                                                                                  |                                                                                                 
 gcATATGGCCGCCTTTGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCa                                                                                                >  2:3995177/1‑101 (MQ=255)
   atatGGCCGCCTTTGACCCCGGAACCGATCTGAACCGCCTGTGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCAcc                                                                                              <  2:1687344/101‑1 (MQ=255)
   atatGGCCGCCTTTGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCAcc                                                                                              >  1:3274848/1‑101 (MQ=255)
    tatGGCCGCCTTTGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCAccc                                                                                             <  2:2352091/101‑1 (MQ=255)
      tGGCCGCCTTTGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCctc                                                                                           >  2:283115/1‑101 (MQ=255)
      tGGCCGCCTTTGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCctc                                                                                           <  2:3703279/101‑1 (MQ=255)
      tGGCCGCCTTTGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCctc                                                                                           <  1:3411253/101‑1 (MQ=255)
       ggCCGCCTTTGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCctct                                                                                          <  1:2883411/101‑1 (MQ=255)
       ggCCGCCTTTGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCctct                                                                                          >  2:1945417/1‑101 (MQ=255)
       ggCCGCCTTTGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCctct                                                                                          <  1:3927337/101‑1 (MQ=255)
        gccgccTTTGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCtctc                                                                                         <  2:536586/101‑1 (MQ=255)
        gccgccTTTGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCtctc                                                                                         <  1:5374/101‑1 (MQ=255)
        gccgccTTTGACCCCGGAACCGATCTGAACCGCCTGCGCCCCGAAATCCTGGCGCAGGTCTGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCtctc                                                                                         >  1:3491367/1‑101 (MQ=255)
         ccgccTTTGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCg                                                                                        <  2:2212931/101‑1 (MQ=255)
          cgccTTTGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCgg                                                                                       >  1:2575989/1‑101 (MQ=255)
          cgccTTTGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCgg                                                                                       <  2:3595205/101‑1 (MQ=255)
            ccTTTGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTc                                                                                     >  1:2174886/1‑101 (MQ=255)
              tttGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTccc                                                                                   <  1:2062974/101‑1 (MQ=255)
              tttGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTccc                                                                                   >  2:1042670/1‑101 (MQ=255)
               ttGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCg                                                                                  >  1:3686695/1‑101 (MQ=255)
                tGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGa                                                                                 <  1:1816839/101‑1 (MQ=255)
                 gACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGAc                                                                                >  1:659716/1‑101 (MQ=255)
                 gACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGAc                                                                                <  2:2718462/101‑1 (MQ=255)
                    cccGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACggg                                                                             >  2:3307769/1‑101 (MQ=255)
                    cccGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACggg                                                                             <  2:1320208/101‑1 (MQ=255)
                     ccGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGc                                                                            <  1:2341791/101‑1 (MQ=255)
                     ccGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGc                                                                            <  2:3862420/101‑1 (MQ=255)
                        gAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCCAGGCCGCACTGCCGCCGGGCCTGTTCACACTCTCGGCCCCGCCGGGCGGt                                                                         >  1:571245/1‑101 (MQ=255)
                        gAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGt                                                                         <  2:1181201/101‑1 (MQ=255)
                         aaCCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTg                                                                        >  2:2708001/1‑101 (MQ=255)
                          aCCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTgg                                                                       <  2:112542/101‑1 (MQ=255)
                          aCCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTgg                                                                       <  1:3191226/101‑1 (MQ=255)
                           ccGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGa                                                                      >  2:3780466/1‑101 (MQ=255)
                           ccGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGa                                                                      >  2:2178661/1‑101 (MQ=255)
                             gATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGaaa                                                                    >  1:2465695/1‑101 (MQ=255)
                              aTCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGaaaa                                                                   >  2:851694/1‑101 (MQ=255)
                                cTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAAc                                                                 <  1:2708207/101‑1 (MQ=255)
                                 tGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAAcc                                                                >  2:2103756/1‑101 (MQ=255)
                                 tGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAAcc                                                                <  1:1344365/101‑1 (MQ=255)
                                  gAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAAccc                                                               <  2:2575798/101‑1 (MQ=255)
                                  gAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAAccc                                                               <  2:451782/101‑1 (MQ=255)
                                   aaCCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCt                                                              >  1:3630375/1‑101 (MQ=255)
                                    aCCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCtt                                                             >  1:3963619/1‑101 (MQ=255)
                                    aCCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCtt                                                             <  1:2739250/101‑1 (MQ=255)
                                    aCCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCtt                                                             >  1:1762053/1‑101 (MQ=255)
                                    aCCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCtt                                                             <  1:1407242/101‑1 (MQ=255)
                                    aCCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCAAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCtt                                                             <  2:1702748/101‑1 (MQ=255)
                                     ccGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGCAAAACCCTTg                                                            >  1:764407/1‑101 (MQ=255)
                                     ccGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTg                                                            <  1:4028609/101‑1 (MQ=255)
                                     ccGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTg                                                            <  2:1019754/101‑1 (MQ=255)
                                      cGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGc                                                           >  1:2322279/1‑101 (MQ=255)
                                      cGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACACTTGc                                                           >  2:2289681/1‑101 (MQ=255)
                                        ccTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCt                                                         <  1:1023468/101‑1 (MQ=255)
                                         cTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTc                                                        >  2:4155694/1‑101 (MQ=255)
                                          tGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCg                                                       <  1:3072083/101‑1 (MQ=255)
                                           gcgcgcCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGc                                                      >  2:292565/1‑101 (MQ=255)
                                              cgcCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTg                                                   <  2:1152706/101‑1 (MQ=255)
                                               gcCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTgg                                                  <  1:851775/101‑1 (MQ=255)
                                                  gAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCtt                                               >  2:802094/1‑101 (MQ=255)
                                                  caaaTCGTGGCGAAGCTCCCGGCCTAGACCGCACCCCCGCCGGGCCTGTTCACCCTCAGGGCCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCtt                                               <  2:571181/100‑1 (MQ=255)
                                                    aaTCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTg                                             <  1:3335936/101‑1 (MQ=255)
                                                    aaTCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTg                                             <  1:3747633/101‑1 (MQ=255)
                                                    aaTCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTg                                             >  2:4168248/1‑101 (MQ=255)
                                                       ccTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTgcgc                                          <  1:292610/101‑1 (MQ=255)
                                                           gcgcAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGa                                      >  2:69127/1‑101 (MQ=255)
                                                            cgcAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGAt                                     >  2:441504/1‑101 (MQ=255)
                                                             gcAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATc                                    >  1:1678095/1‑101 (MQ=255)
                                                               aGGTCCGGGCCAAGGCCGCACTTCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCAc                                  <  1:4155956/101‑1 (MQ=255)
                                                               aGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCAc                                  >  2:223566/1‑101 (MQ=255)
                                                               aGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCAc                                  >  1:926167/1‑101 (MQ=255)
                                                                 gTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCAcgc                                >  1:2345693/1‑101 (MQ=255)
                                                                 gTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCAcgc                                >  2:3044385/1‑101 (MQ=255)
                                                                 gTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCAcgc                                >  2:1922221/1‑101 (MQ=255)
                                                                  tCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCAcgcg                               <  2:3397851/101‑1 (MQ=255)
                                                                  tCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCAcgcg                               <  1:3838883/101‑1 (MQ=255)
                                                                   ccGGGCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACgcgg                              >  1:1915675/1‑101 (MQ=255)
                                                                     gggCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACgcggcg                            >  2:2484424/1‑101 (MQ=255)
                                                                       gCCAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCTGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTg                          <  1:3581893/101‑1 (MQ=255)
                                                                         cAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGcc                        >  2:269612/1‑101 (MQ=255)
                                                                         cAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGcc                        <  1:4168510/101‑1 (MQ=255)
                                                                         cAAGGCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGAGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGcc                        >  2:1854319/1‑101 (MQ=255)
                                                                             gCCGCACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACgg                    <  2:2133120/101‑1 (MQ=255)
                                                                                 cACTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACGGGCAt                >  2:2335759/1‑101 (MQ=255)
                                                                                  aCTGCCGCCGGGCCTGTTCACCCTCTCGGTNCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACGGGCATc               <  1:2289849/101‑1 (MQ=255)
                                                                                   cTGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACGGGCATcg              <  1:3368767/101‑1 (MQ=255)
                                                                                    tGCCGCCGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACGGGCATcgg             >  2:1065353/1‑101 (MQ=255)
                                                                                     gccgccGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACGGGCATcggc            >  2:3238552/1‑101 (MQ=255)
                                                                                     gccgccGGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACGGGCATcggc            >  2:2943705/1‑101 (MQ=255)
                                                                                      ccgccgGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACGGGCATcggcg           >  2:3091001/1‑101 (MQ=255)
                                                                                      ccgccgGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACGGGCATcggcg           <  1:69142/101‑1 (MQ=255)
                                                                                       cgccgGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACGGGCATcggcgg          >  2:240192/1‑101 (MQ=255)
                                                                                         ccgGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACGGGCATCGGCGGat        <  1:1903718/101‑1 (MQ=255)
                                                                                         ccgGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACGGGCATCGGCGGat        >  1:1491802/1‑101 (MQ=255)
                                                                                          cgGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACGGGCATCGGCGGatc       <  1:1042764/101‑1 (MQ=255)
                                                                                          cgGGCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACGGGCATCGGCGGatc       >  2:1373773/1‑101 (MQ=255)
                                                                                           gggCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACGGGCATCGGCGGatca      >  2:2475867/1‑101 (MQ=255)
                                                                                           gggCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACGGGCATCGGCGGatca      >  2:1001558/1‑101 (MQ=255)
                                                                                            ggCCTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACGGGCATCGGCGGatcat     >  2:882980/1‑101 (MQ=255)
                                                                                              ccTGTTCACCCTCTCGGGCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACGGGCATCGGCGGATCATCt   <  1:4065699/101‑1 (MQ=255)
                                                                                               cTGTTCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACTCGGCGTGCCACGGGCATCGGCGGATCATCTa  <  1:3790460/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CGCATATGGCCGCCTTTGACCCCGGAACCGATCTGAACCGCCTGCGCGCCGAAATCCTGGCGCAGGTCCGGGCCAAGGCCGCACTGCCGCCGGGCCTGATCACCCTCTCGGTCCCGACGGGCGGTGGAAAAACCCTTGCCTCGCTTGGCTTTGCGCTGGATCACGCGGCGTGCCACGGGCATCGGCGGATCATCTA  >  NC_014034/1388085‑1388280

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG