Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,423 G→T 2.1% intergenic (+235/‑696) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,4230G→T 2.1% 3.4 0.0 ‑0.5194intergenic (+235/‑696)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (93/97):  total (95/99)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.45e-01

Only 100 of 231 total aligned reads displayed.

GAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1395324‑1395523
                                                                                                   |                                                                                                     
gAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatcgg                                                                                                      >  2:3678724/1‑101 (MQ=255)
  gCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatcggtg                                                                                                    <  2:1777732/101‑1 (MQ=255)
    aacttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatcggtg.at                                                                                                 <  2:3564004/98‑1 (MQ=255)
      tttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCa                                                                                               <  2:754864/101‑1 (MQ=255)
       ttACACCGTCTGGTCAGCGGGTTACGAATCGGCAGTCGCTCCCCCCGCGGGGGCGTGGATGGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAg                                                                                              <  2:57269/101‑1 (MQ=255)
       ttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAg                                                                                              >  1:972086/1‑101 (MQ=255)
       ttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAg                                                                                              <  1:1835958/101‑1 (MQ=255)
         acacCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCg                                                                                            <  1:1774483/101‑1 (MQ=255)
         acacCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCg                                                                                            >  2:1015597/1‑101 (MQ=255)
           acCGTCTGTTCAGCGGGTTACGAAACTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCACCCGCCGGCCCTGCCGGTGATCCGTG.ATCAGCGGt                                                                                          >  2:1341115/1‑101 (MQ=37)
           acCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGt                                                                                          <  2:918435/101‑1 (MQ=255)
           acCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGt                                                                                          <  2:3074321/101‑1 (MQ=255)
           acCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCCGTGATCGGCG.ATCAGCGGt                                                                                          >  2:558277/1‑101 (MQ=255)
            ccGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTc                                                                                         <  1:2444689/101‑1 (MQ=255)
             cGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCg                                                                                        >  2:516468/1‑101 (MQ=255)
              gTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGc                                                                                       >  2:1283151/1‑101 (MQ=255)
                cTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTc                                                                                     >  1:1205297/1‑101 (MQ=255)
                 tGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTcc                                                                                    <  2:2328995/101‑1 (MQ=255)
                  ggTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTccc                                                                                   <  2:3457476/101‑1 (MQ=255)
                  ggTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTccc                                                                                   <  1:327732/101‑1 (MQ=255)
                  ggTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTccc                                                                                   <  1:2143436/101‑1 (MQ=255)
                    tCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTccccc                                                                                 <  1:2856020/101‑1 (MQ=255)
                    tCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTccccc                                                                                 <  2:2099326/101‑1 (MQ=255)
                    tCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTccccc                                                                                 >  2:2514858/1‑101 (MQ=255)
                    tCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTccccc                                                                                 >  2:2829609/1‑101 (MQ=255)
                    tCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTccccc                                                                                 >  1:1676851/1‑101 (MQ=255)
                     cAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTcccccc                                                                                >  2:147668/1‑101 (MQ=255)
                      aGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCcg                                                                               <  1:634008/101‑1 (MQ=255)
                         gggTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCgg                                                                            >  1:3870414/1‑101 (MQ=255)
                         gggTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCgg                                                                            <  2:2575811/101‑1 (MQ=255)
                          ggTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCggg                                                                           <  2:1672405/101‑1 (MQ=255)
                           gTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.GTCAGCGGTCGCTCCCCCCGCgggg                                                                          >  2:2726244/1‑101 (MQ=255)
                           gTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCgggg                                                                          <  1:4068571/101‑1 (MQ=255)
                             tACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGc                                                                        <  2:2214065/101‑1 (MQ=255)
                              aCGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCg                                                                       >  2:4122522/1‑101 (MQ=255)
                              aCGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCg                                                                       <  1:799897/101‑1 (MQ=255)
                                gAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTg                                                                     <  1:2663286/101‑1 (MQ=255)
                                 aaaCGGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTgc                                                                    <  1:993535/101‑2 (MQ=255)
                                 aaaCGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTgg                                                                    >  1:2539234/1‑101 (MQ=255)
                                  caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                 <  2:2522771/94‑1 (MQ=2)
                                     cactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                              >  2:822815/5‑101 (MQ=2)
                                        aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACTCGCTGGCCCTGCCGGAGATCGGTG.ATCAGCGGTCGCCGCCCCCGCGGGGGCGTGGATCCaaa                                                             >  2:2475671/1‑101 (MQ=37)
                                        aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                             >  2:2462013/1‑101 (MQ=255)
                                         gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                            <  2:2082292/101‑1 (MQ=255)
                                         gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                            <  2:3645090/101‑1 (MQ=255)
                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                          >  1:3346848/1‑101 (MQ=255)
                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACAt                                                          >  2:3875887/1‑101 (MQ=255)
                                             cgccccccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                        <  1:3776387/99‑1 (MQ=255)
                                             cTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                        >  2:2915915/1‑101 (MQ=255)
                                             cTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                        >  1:2075439/1‑101 (MQ=255)
                                             cTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                        <  1:3040077/101‑1 (MQ=255)
                                             cTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                        <  2:3146514/101‑1 (MQ=255)
                                              cccccccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgc                                                       <  2:1075172/100‑1 (MQ=255)
                                                  cccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCt                                                   >  1:853253/1‑101 (MQ=255)
                                                   ccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCtt                                                  >  1:204845/1‑101 (MQ=255)
                                                   ccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCtt                                                  >  1:2250795/1‑101 (MQ=255)
                                                   ccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCtt                                                  <  2:1891112/101‑1 (MQ=255)
                                                    cgcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCttt                                                 >  1:180940/1‑101 (MQ=255)
                                                    cgcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCttt                                                 <  1:1499625/101‑1 (MQ=255)
                                                     gcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTg                                                <  1:1225667/101‑1 (MQ=255)
                                                     gcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTg                                                >  1:555843/1‑101 (MQ=255)
                                                     gcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCGCCGCTTTg                                                >  2:3367674/1‑101 (MQ=255)
                                                           gCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTc                                          >  1:261755/1‑101 (MQ=255)
                                                            cGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCg                                         >  1:2938501/1‑101 (MQ=255)
                                                            cGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCg                                         >  1:2438469/1‑101 (MQ=255)
                                                             gTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGa                                        >  1:3875017/1‑101 (MQ=255)
                                                               ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACa                                      >  1:1626108/1‑101 (MQ=255)
                                                                gATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAg                                     >  2:3913696/1‑101 (MQ=255)
                                                                gATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAg                                     <  2:3716934/101‑1 (MQ=255)
                                                                 aTCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAgg                                    >  2:117086/1‑101 (MQ=255)
                                                                   cGAACCTGCACCCGCTGGCCCTGCCGGTGAACGGTG.ATCAGCGGTCCCGCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCt                                  <  2:3503070/101‑1 (MQ=38)
                                                                       aCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTa                              <  2:4210035/101‑1 (MQ=255)
                                                                       aCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTa                              <  1:3466905/101‑1 (MQ=255)
                                                                        cTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTaa                             <  2:3003445/101‑1 (MQ=255)
                                                                         tGCCCCCGCTGCCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAc                            <  1:4159381/101‑1 (MQ=255)
                                                                         tGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAc                            <  1:3913955/101‑1 (MQ=255)
                                                                          gCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACg                           <  2:2730245/101‑1 (MQ=255)
                                                                           cccccGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACgt                          <  2:1625999/99‑1 (MQ=255)
                                                                            ccccGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACgtg                         <  1:3971508/100‑1 (MQ=255)
                                                                             cccGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACgtgt                        <  1:516525/101‑1 (MQ=255)
                                                                              ccGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTc                       >  2:3356045/1‑101 (MQ=255)
                                                                              ccGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGGAACGTGTc                       >  2:1749675/1‑101 (MQ=255)
                                                                               cGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCg                      >  2:329753/1‑101 (MQ=255)
                                                                                gCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGc                     >  2:3405164/1‑101 (MQ=255)
                                                                                   ggCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCGTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcc                  >  1:3676267/1‑101 (MQ=255)
                                                                                     cccTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccc                >  2:4022521/1‑101 (MQ=255)
                                                                                      ccTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTccccc               <  2:2938279/101‑1 (MQ=255)
                                                                                      ccTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTccccc               <  1:100034/101‑1 (MQ=255)
                                                                                       cTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccccc              <  2:1587702/101‑1 (MQ=255)
                                                                                       cTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccccc              <  1:785583/101‑1 (MQ=255)
                                                                                       cTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccccc              <  1:1279773/101‑1 (MQ=255)
                                                                                         gCCCGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcgc            <  2:4167790/101‑1 (MQ=255)
                                                                                          ccGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcccg           >  1:3139498/1‑101 (MQ=255)
                                                                                             gtgatcggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgggg        >  1:3601996/1‑101 (MQ=255)
                                                                                              tgatcggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCggggg       >  2:3176516/1‑101 (MQ=255)
                                                                                               gatcggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc      <  2:1922950/101‑1 (MQ=255)
                                                                                               gatcggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc      <  2:971995/101‑1 (MQ=255)
                                                                                                atcggtg.atcAGCGGTCGCTCCCCCCGCGGGGGTTTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCg     <  1:3630275/101‑1 (MQ=255)
                                                                                                atcggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCg     <  1:2183852/101‑1 (MQ=255)
                                                                                                  gggtg.gtgAGGGGCCGCTCCGCCCGCGGGGGCGGGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTg   <  2:1188078/100‑1 (MQ=38)
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GAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1395324‑1395523

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG