Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,466 A→T 18.1% intergenic (+278/‑653) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,4660A→T18.1% 137.6 ‑1.1 ‑0.1257intergenic (+278/‑653)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (18/28):  ref base (100/109):  total (118/139)
Fisher's exact test strand distribution p-value = 3.29e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.82e-01

Only 100 of 269 total aligned reads displayed.

TCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTC  >  NC_014034/1395366‑1395566
                                                                                                    |                                                                                                        
 cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                                                                         >  1:3346848/1‑101 (MQ=255)
  gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                                                                        >  1:1406727/1‑101 (MQ=255)
   ccccccccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                                                                       <  1:3048061/99‑1 (MQ=255)
   cTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                                                                       <  2:3146514/101‑1 (MQ=255)
     ccccccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgcc                                                                                                     <  2:3543428/101‑1 (MQ=255)
        cccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...Ct                                                                                               >  1:853253/1‑101 (MQ=255)
        cccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...Ct                                                                                               >  1:2167976/1‑101 (MQ=255)
         ccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...Ct.t                                                                                             >  1:204845/1‑101 (MQ=255)
          cgcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...Ct.tt                                                                                            <  1:1499625/101‑1 (MQ=255)
          cgcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...Ct.tt                                                                                            >  1:180940/1‑101 (MQ=255)
            cgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTgg                                                                                          >  2:2921948/1‑101 (MQ=255)
               gggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCgg                                                                                       >  1:3638668/1‑101 (MQ=255)
                   gTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGa                                                                                   >  2:848418/1‑101 (MQ=255)
                   gTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGa                                                                                   >  1:3875017/1‑101 (MQ=255)
                    tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGAc                                                                                  >  1:2220055/1‑101 (MQ=255)
                     ggATCGAAACTGCACCCGCTGGCCCTGCCTGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACa                                                                                 >  1:2624157/1‑101 (MQ=255)
                     ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACa                                                                                 >  1:1626108/1‑101 (MQ=255)
                     ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACa                                                                                 >  1:2443341/1‑101 (MQ=255)
                         cGAACCTGCACCCGCTGGCCCTGCCGGTGAACGGTGATCAGCGGTCCCGCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCt                                                                             <  2:3503070/101‑1 (MQ=38)
                               tGCCCCCGCTGCCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAAc                                                                       <  1:4159381/101‑1 (MQ=255)
                               cgCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAAc                                                                       <  1:558341/100‑1 (MQ=255)
                                gCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACg                                                                      >  1:1723007/1‑101 (MQ=255)
                                  ccccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACgtg                                                                    <  1:3971508/100‑1 (MQ=255)
                                  aCCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGGAACgtg                                                                    >  1:587742/1‑101 (MQ=255)
                                    ccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTc                                                                  >  2:3356045/1‑101 (MQ=255)
                                    ccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTc                                                                  <  1:2726905/101‑1 (MQ=255)
                                    ccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGGAACGTGTc                                                                  >  2:1749675/1‑101 (MQ=255)
                                     cGCTGGCCCTGCCGGTGATCGGTGTTCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCg                                                                 >  2:459267/1‑101 (MQ=255)
                                     cGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCg                                                                 >  2:329753/1‑101 (MQ=255)
                                     cGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCg                                                                 <  1:1212377/101‑1 (MQ=255)
                                      gCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGc                                                                >  2:3405164/1‑101 (MQ=255)
                                       cTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCt                                                               >  1:4207508/1‑101 (MQ=255)
                                        tGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTc                                                              >  2:3625845/1‑101 (MQ=255)
                                         ggCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGNTGGTAACGTGTCGCTcc                                                             >  2:1049215/1‑101 (MQ=255)
                                         ggCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTcc                                                             <  2:2875529/101‑1 (MQ=255)
                                          gctgtGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTccc                                                            <  1:295384/97‑1 (MQ=255)
                                            ccTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTccccc                                                          <  1:100034/101‑1 (MQ=255)
                                             cTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccccc                                                         <  1:785583/101‑1 (MQ=255)
                                             cTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccccc                                                         <  2:1587702/101‑1 (MQ=255)
                                                   gtgatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgggg                                                   >  1:3601996/1‑101 (MQ=255)
                                                     gatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc                                                 <  2:971995/101‑1 (MQ=255)
                                                     gatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc                                                 <  2:1922950/101‑1 (MQ=255)
                                                       tcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGt                                               <  2:4177425/101‑1 (MQ=255)
                                                        gggtggtgAGGGGCCGCTCCGCCCGCGGGGGCGGGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTg                                              <  2:1188078/100‑1 (MQ=38)
                                                          gtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGa                                            >  2:3691073/1‑101 (MQ=255)
                                                          gtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGa                                            <  2:4127914/101‑1 (MQ=255)
                                                          gtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGa                                            >  1:3950676/1‑101 (MQ=255)
                                                          gtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGa                                            >  1:107331/1‑101 (MQ=255)
                                                          gctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGAt                                           <  2:2052707/92‑1 (MQ=2)
                                                           ctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATc                                          >  1:546449/9‑101 (MQ=0)
                                                           ctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATc                                          >  1:1539092/9‑101 (MQ=0)
                                                           ctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATc                                          >  2:3481168/9‑101 (MQ=0)
                                                            tggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                         <  1:3782888/94‑1 (MQ=2)
                                                            gatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATc                                          <  2:555779/101‑1 (MQ=255)
                                                              gacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                       <  2:3929102/96‑1 (MQ=2)
                                                              gacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                       >  2:3206203/6‑101 (MQ=2)
                                                              gacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                       <  2:3044448/96‑1 (MQ=2)
                                                               cAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                       >  2:3891808/1‑101 (MQ=255)
                                                               cAGCGGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                       <  2:271902/101‑1 (MQ=255)
                                                               acgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                      >  2:2748688/5‑101 (MQ=2)
                                                                aGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                      <  1:1283247/101‑1 (MQ=255)
                                                                 gtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                    <  1:2609604/99‑1 (MQ=2)
                                                                 gtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                    >  1:4108360/3‑101 (MQ=2)
                                                                 gCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                     >  2:1731691/1‑101 (MQ=255)
                                                                  tggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACag                                   <  2:1564782/100‑2 (MQ=2)
                                                                   ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACagc                                  <  1:3625243/101‑3 (MQ=2)
                                                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtct                                 >  2:2483019/1‑101 (MQ=255)
                                                                      ngccncccccGCGGGGGCGTGGATCGAAAC.TCCCCGAACCTGTCGGCGG.CGA.ACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtcg                                 <  1:3196898/96‑2 (MQ=0)
                                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtctc                                <  1:3457309/101‑1 (MQ=255)
                                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtctc                                <  1:2853850/101‑1 (MQ=255)
                                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttc                              <  1:993780/101‑4 (MQ=2)
                                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCg                               >  1:831056/1‑101 (MQ=255)
                                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCg                               <  1:459324/101‑1 (MQ=255)
                                                                        cTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttca                             <  1:1309471/101‑5 (MQ=2)
                                                                         tCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGt                             >  1:2359869/1‑101 (MQ=255)
                                                                          ccccccGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaat                           >  2:145188/1‑95 (MQ=0)
                                                                          ccccccGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaat                           <  1:1767511/101‑7 (MQ=0)
                                                                           cccccGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGc                           <  1:1897925/101‑1 (MQ=255)
                                                                           cccccGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGGGc                           >  2:2952045/1‑101 (MQ=255)
                                                                            ccccGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaatgg                         <  2:1083294/101‑9 (MQ=2)
                                                                             cccGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCg                         <  1:3261659/101‑1 (MQ=255)
                                                                                  gggggCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGcc                    >  2:1988290/1‑100 (MQ=255)
                                                                                     ggCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCCGTGCCGTCGCTCGt                 >  2:4113630/1‑101 (MQ=255)
                                                                                       cGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTTGATCGAAACATCTCGGTGCCGTCGCTCGTcc               <  1:3754861/101‑1 (MQ=255)
                                                                                       cGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTcc               <  1:1947437/101‑1 (MQ=255)
                                                                                        gTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCa              <  2:3829519/101‑1 (MQ=255)
                                                                                         tGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAg             <  2:2590120/101‑1 (MQ=255)
                                                                                         tGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAg             <  2:2219896/101‑1 (MQ=255)
                                                                                          ggATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGc            >  2:1836906/1‑101 (MQ=255)
                                                                                            aTCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCg          >  2:3402862/1‑101 (MQ=255)
                                                                                              cGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCCGc        <  1:3128442/101‑1 (MQ=255)
                                                                                               gAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGcc       >  2:3085897/1‑101 (MQ=255)
                                                                                                aagcATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCt      >  1:2418477/4‑101 (MQ=255)
                                                                                                aaaCATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCt      <  2:587678/101‑1 (MQ=255)
                                                                                                aaaCATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCt      <  2:2438293/101‑1 (MQ=255)
                                                                                                 aaCATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTg     <  2:2167831/101‑1 (MQ=255)
                                                                                                 aaCATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTg     <  1:2257267/101‑1 (MQ=255)
                                                                                                  aCATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGc    >  2:630643/1‑101 (MQ=255)
                                                                                                    aTCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtc  <  2:1626572/101‑1 (MQ=255)
                                                                                                    aTCGCCG...CT.TTGGCGGTCGACAGCCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtc  <  2:544165/101‑1 (MQ=255)
                                                                                                    |                                                                                                        
TCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTC  >  NC_014034/1395366‑1395566

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG