BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,395,473	C→G	19.2%	intergenic (+285/‑646)	RCAP_rcc01306 → / → RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,395,473	0	C→G	19.2%	122.4 ‑1.1 ‑0.4	245	intergenic (+285/‑646)	RCAP_rcc01306/RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (18/29): ref base (93/105): total (111/134)
Fisher's exact test strand distribution p-value = 3.29e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.25e-01

Only 100 of 259 total aligned reads displayed.


CCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTC  >  NC_014034/1395374‑1395573


cccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...Ct                                                                                                      >  1:853253/1‑101 (MQ=255)


cccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...Ct                                                                                                      <  2:334183/101‑1 (MQ=255)


 ccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...Ct.t                                                                                                    <  2:178013/101‑1 (MQ=255)


 ccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...Ct.t                                                                                                    <  2:1891112/101‑1 (MQ=255)


 ccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...Ct.t                                                                                                    >  1:2250795/1‑101 (MQ=255)


  cgcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...Ct.tt                                                                                                   <  1:1499625/101‑1 (MQ=255)


  cgcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...Ct.tt                                                                                                   >  2:4159119/1‑101 (MQ=255)


   gcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTg                                                                                                  >  2:158354/1‑101 (MQ=255)


   gcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTg                                                                                                  >  1:555843/1‑101 (MQ=255)


   gcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTg                                                                                                  <  2:2654007/101‑1 (MQ=255)


   gcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTg                                                                                                  <  2:3152866/101‑1 (MQ=255)


   gcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTg                                                                                                  <  1:3062779/101‑1 (MQ=255)


     gggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGc                                                                                                <  2:550473/101‑1 (MQ=255)


        ggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGt                                                                                             >  1:4127044/1‑101 (MQ=255)


        ggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGt                                                                                             >  2:3678061/1‑101 (MQ=255)


         gCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTc                                                                                            >  1:261755/1‑101 (MQ=255)


          cGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCg                                                                                           >  1:2438469/1‑101 (MQ=255)


          cGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCg                                                                                           >  2:3457057/1‑101 (MQ=255)


          cGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCg                                                                                           >  1:2938501/1‑101 (MQ=255)


           gTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGa                                                                                          >  2:848418/1‑101 (MQ=255)


           gTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGa                                                                                          >  1:3875017/1‑101 (MQ=255)


             ggATCGAAACTGCACCCGCTGGCCCTGCCTGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACa                                                                                        >  1:2624157/1‑101 (MQ=255)


             ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACa                                                                                        >  1:988216/1‑101 (MQ=255)


             ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACa                                                                                        >  2:3219197/1‑101 (MQ=255)


             ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACa                                                                                        >  1:1626108/1‑101 (MQ=255)


              gATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAg                                                                                       >  2:3913696/1‑101 (MQ=255)


              gATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAg                                                                                       <  2:3716934/101‑1 (MQ=255)


                 cGAACCTGCACCCGCTGGCCCTGCCGGTGAACGGTGATCAGCGGTCCCGCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCt                                                                                    <  2:3503070/101‑1 (MQ=38)


                   aaaCTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGGCGACAGGCTgg                                                                                  >  1:2875748/1‑101 (MQ=255)


                     aCTGCCCCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTa                                                                                <  1:814588/101‑1 (MQ=255)


                     aCTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTa                                                                                <  2:4210035/101‑1 (MQ=255)


                     aCTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTa                                                                                <  1:3466905/101‑1 (MQ=255)


                       tGCCCCCGCTGCCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAAc                                                                              <  1:4159381/101‑1 (MQ=255)


                       tGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAAc                                                                              <  1:3913955/101‑1 (MQ=255)


                        gCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACg                                                                             >  2:2416645/1‑101 (MQ=255)


                        gCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACg                                                                             >  1:1723007/1‑101 (MQ=255)


                         cccccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACgt                                                                            <  2:1625999/99‑1 (MQ=255)


                          aCCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGGAACgtg                                                                           >  1:587742/1‑101 (MQ=255)


                            ccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGTGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTc                                                                         <  2:335861/101‑1 (MQ=255)


                             cGCTGGCCCTGCCGGTGATCGGTGTTCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCg                                                                        >  2:459267/1‑101 (MQ=255)


                             cGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCg                                                                        <  1:1212377/101‑1 (MQ=255)


                              gCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGGAACGTGTCGc                                                                       >  2:3001589/1‑101 (MQ=255)


                                 ggCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTcc                                                                    <  2:2875529/101‑1 (MQ=255)


                                    ccTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTccccc                                                                 <  1:100034/101‑1 (MQ=255)


                                    ccTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTccccc                                                                 <  2:2938279/101‑1 (MQ=255)


                                     cTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccccc                                                                <  1:1279773/101‑1 (MQ=255)


                                     cTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccccc                                                                <  1:785583/101‑1 (MQ=255)


                                           gtgatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgggg                                                          >  1:3601996/1‑101 (MQ=255)


                                           gtgatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgggg                                                          <  1:2392989/101‑1 (MQ=255)


                                             gatcggtgatcAGCGGTCGCTCCCCCCGCGTGGGCGTGGATCGAAACATCGCCG...CT.TTGGCAGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGTGc                                                        <  2:3481269/101‑1 (MQ=255)


                                             gatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc                                                        <  2:1922950/101‑1 (MQ=255)


                                             gatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc                                                        >  2:566937/1‑101 (MQ=255)


                                             gatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc                                                        <  2:971995/101‑1 (MQ=255)


                                              atcggtgatcAGCGGTCGCTCCCCCCGCGGGGGTTTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCg                                                       <  1:3630275/101‑1 (MQ=255)


                                              atcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCg                                                       <  1:2183852/101‑1 (MQ=255)


                                                gggtggtgAGGGGCCGCTCCGCCCGCGGGGGCGGGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTg                                                     <  2:1188078/100‑1 (MQ=38)


                                                cggtgatcAGCGGACGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTg                                                     <  2:3089563/101‑1 (MQ=255)


                                                 ggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTgg                                                    >  1:1832167/1‑101 (MQ=255)


                                                  gtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGa                                                   >  1:107331/1‑101 (MQ=255)


                                                   tgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGAt                                                  <  1:157014/101‑1 (MQ=255)


                                                   ctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                 >  1:546449/9‑101 (MQ=0)


                                                     ggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                               >  2:1743447/7‑101 (MQ=2)


                                                     ggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGCGGATCGa                                               >  2:4121746/7‑101 (MQ=2)


                                                      gacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                              <  2:3044448/96‑1 (MQ=2)


                                                       cAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                              >  2:3891808/1‑101 (MQ=255)


                                                       cAGCGGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                              <  2:271902/101‑1 (MQ=255)


                                                       acgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                             >  2:2748688/5‑101 (MQ=2)


                                                        cgtggTCGCTCCCCCCGCGGGGGGGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                            >  1:398216/4‑101 (MQ=2)


                                                          tggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACag                                          >  1:2314939/2‑100 (MQ=2)


                                                          tggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACag                                          <  2:1564782/100‑2 (MQ=2)


                                                          cGGTCGCTCCCCCCGCGGGGGCGTGGAGCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                           <  1:3367919/101‑1 (MQ=255)


                                                           ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACagc                                         >  2:640582/1‑99 (MQ=2)


                                                             tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtct                                        >  2:2483019/1‑101 (MQ=255)


                                                              ngccncccccGCGGGGGCGTGGATCGAAAC.TCCCCGAACCTGTCGGCGG.CGA.ACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtcg                                        <  1:3196898/96‑2 (MQ=0)


                                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttc                                     >  1:1979495/1‑98 (MQ=2)


                                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCg                                      <  1:459324/101‑1 (MQ=255)


                                                                cTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttca                                    <  1:1309471/101‑5 (MQ=2)


                                                                 tCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGt                                    >  1:2066742/1‑101 (MQ=255)


                                                                  ccccccGCGGGGGTGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTg                                   <  2:1047259/101‑1 (MQ=255)


                                                                  ccccccGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaat                                  <  1:1767511/101‑7 (MQ=0)


                                                                  ccccccGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaat                                  <  1:2748894/101‑7 (MQ=0)


                                                                  ccccccGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaat                                  >  2:145188/1‑95 (MQ=0)


                                                                   cccccGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGc                                  <  1:2472404/101‑1 (MQ=255)


                                                                     cccGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCg                                <  1:3261659/101‑1 (MQ=255)


                                                                           ggggCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGGCACTc                          >  2:1153172/1‑101 (MQ=255)


                                                                             ggCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGt                        <  2:2075301/101‑1 (MQ=255)


                                                                             ggCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCTCGGTGCCGTCGCTCgc                        >  1:422710/1‑100 (MQ=255)


                                                                              gCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTc                       <  2:3404148/101‑1 (MQ=255)


                                                                               cGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTTGATCGAAACATCTCGGTGCCGTCGCTCGTcc                      <  1:3754861/101‑1 (MQ=255)


                                                                                gTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCa                     >  1:1936922/1‑101 (MQ=255)


                                                                                 tGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAg                    <  1:1341215/101‑1 (MQ=255)


                                                                                 tGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAg                    <  2:2219896/101‑1 (MQ=255)


                                                                                    aTCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCg                 >  2:3624506/1‑101 (MQ=255)


                                                                                    aTCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCg                 <  2:1655313/101‑1 (MQ=255)


                                                                                      cGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCCGc               <  1:3128442/101‑1 (MQ=255)


                                                                                        aaaCATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCt             <  2:587678/101‑1 (MQ=255)


                                                                                           cATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCt          >  2:2007715/1‑101 (MQ=255)


                                                                                               gccg...cT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtcgtc      <  2:393811/101‑1 (MQ=255)


                                                                                               gccg...cT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtcgtc      <  2:2066604/101‑1 (MQ=255)


                                                                                                      cT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTc  <  1:3725765/101‑1 (MQ=255)


CCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTC  >  NC_014034/1395374‑1395573

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG