BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,395,474	T→G	19.6%	intergenic (+286/‑645)	RCAP_rcc01306 → / → RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,395,474	0	T→G	19.6%	130.0 ‑0.7 ‑0.2	242	intergenic (+286/‑645)	RCAP_rcc01306/RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (19/29): ref base (88/106): total (107/135)
Fisher's exact test strand distribution p-value = 5.19e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.59e-01

Only 100 of 262 total aligned reads displayed.


CCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCC  >  NC_014034/1395374‑1395574


 ccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...Ct.t                                                                                                     >  1:204845/1‑101 (MQ=255)


  cgcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...Ct.tt                                                                                                    >  2:4159119/1‑101 (MQ=255)


  cgcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...Ct.tt                                                                                                    >  1:180940/1‑101 (MQ=255)


  cgcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...Ct.tt                                                                                                    <  1:1499625/101‑1 (MQ=255)


   gcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTg                                                                                                   <  2:3152866/101‑1 (MQ=255)


   gcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTg                                                                                                   <  1:3062779/101‑1 (MQ=255)


   gcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTg                                                                                                   <  2:1919368/101‑1 (MQ=255)


   gcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTg                                                                                                   >  1:555843/1‑101 (MQ=255)


    cgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTgg                                                                                                  >  2:2921948/1‑101 (MQ=255)


     gggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGc                                                                                                 <  2:550473/101‑1 (MQ=255)


         gCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTc                                                                                             >  1:261755/1‑101 (MQ=255)


          cGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCg                                                                                            >  1:2938501/1‑101 (MQ=255)


          cGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCg                                                                                            >  2:3457057/1‑101 (MQ=255)


           gTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGa                                                                                           >  2:848418/1‑101 (MQ=255)


           gTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGa                                                                                           >  1:544229/1‑101 (MQ=255)


            tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGAc                                                                                          >  2:2183707/1‑101 (MQ=255)


            tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGAc                                                                                          >  1:2220055/1‑101 (MQ=255)


             ggATCGAAACTGCACCCGCTGGCCCTGCCTGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACa                                                                                         >  1:2624157/1‑101 (MQ=255)


             ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACa                                                                                         <  1:3741846/101‑1 (MQ=255)


             ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACa                                                                                         >  1:988216/1‑101 (MQ=255)


             ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACa                                                                                         >  1:1626108/1‑101 (MQ=255)


              gATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAg                                                                                        <  2:3716934/101‑1 (MQ=255)


              gATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAg                                                                                        >  2:3913696/1‑101 (MQ=255)


                 cGAACCTGCACCCGCTGGCCCTGCCGGTGAACGGTGATCAGCGGTCCCGCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCt                                                                                     <  2:3503070/101‑1 (MQ=38)


                  gAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTg                                                                                    <  1:848362/101‑1 (MQ=255)


                     aCTGCCCCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTa                                                                                 <  1:814588/101‑1 (MQ=255)


                     aCTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTa                                                                                 <  2:4210035/101‑1 (MQ=255)


                      cTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTaa                                                                                <  2:3003445/101‑1 (MQ=255)


                        gCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACg                                                                              >  1:1723007/1‑101 (MQ=255)


                        gCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACg                                                                              <  2:2730245/101‑1 (MQ=255)


                          ccccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACgtg                                                                            <  1:3971508/100‑1 (MQ=255)


                            ccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTc                                                                          >  2:3356045/1‑101 (MQ=255)


                            ccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGGAACGTGTc                                                                          >  2:1749675/1‑101 (MQ=255)


                             cGCTGGCCCTGCCGGTGATCGGTGTTCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCg                                                                         >  2:459267/1‑101 (MQ=255)


                             cGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCg                                                                         <  1:1212377/101‑1 (MQ=255)


                               cTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCt                                                                       >  1:4207508/1‑101 (MQ=255)


                               cTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCt                                                                       <  2:1713654/101‑1 (MQ=255)


                                 ggCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTcc                                                                     <  2:2875529/101‑1 (MQ=255)


                                 ggCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTcc                                                                     <  2:3311789/101‑1 (MQ=255)


                                 ggCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CG.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTcc                                                                     >  1:3676267/1‑101 (MQ=255)


                                    ccTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTccccc                                                                  <  1:100034/101‑1 (MQ=255)


                                     cTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccccc                                                                 <  2:1587702/101‑1 (MQ=255)


                                     cTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccccc                                                                 <  1:1279773/101‑1 (MQ=255)


                                           gtgatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgggg                                                           <  1:2392989/101‑1 (MQ=255)


                                             gatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc                                                         >  2:1330952/1‑101 (MQ=255)


                                             gatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc                                                         <  2:971995/101‑1 (MQ=255)


                                              atcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCg                                                        <  1:2183852/101‑1 (MQ=255)


                                                gggtggtgAGGGGCCGCTCCGCCCGCGGGGGCGGGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTg                                                      <  2:1188078/100‑1 (MQ=38)


                                                  gtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGa                                                    >  1:107331/1‑101 (MQ=255)


                                                  gctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                   <  2:2052707/92‑1 (MQ=2)


                                                   tgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGAt                                                   <  1:157014/101‑1 (MQ=255)


                                                   tgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGAt                                                   <  1:7210/101‑1 (MQ=255)


                                                   ctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCCACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                  <  2:2074133/93‑1 (MQ=2)


                                                   ctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                  >  2:3481168/9‑101 (MQ=0)


                                                    gatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                  <  2:555779/101‑1 (MQ=255)


                                                     atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                 <  1:3876146/101‑1 (MQ=255)


                                                     atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                 >  2:366066/1‑101 (MQ=255)


                                                      gacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                               >  2:3206203/6‑101 (MQ=2)


                                                         gtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                            >  1:4108360/3‑101 (MQ=2)


                                                           ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACagc                                          >  2:640582/1‑99 (MQ=2)


                                                           ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                           <  2:2443157/101‑1 (MQ=255)


                                                             tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctt                                        <  1:3858616/101‑2 (MQ=2)


                                                              ngccncccccGCGGGGGCGTGGATCGAAAC.TCCCCGAACCTGTCGGCGG.CGA.ACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtcg                                         <  1:3196898/96‑2 (MQ=0)


                                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgcttt                                       <  1:41447/101‑3 (MQ=2)


                                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtctc                                        <  1:2853850/101‑1 (MQ=255)


                                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtctc                                        <  1:3457309/101‑1 (MQ=255)


                                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtctc                                        <  1:5830/101‑1 (MQ=255)


                                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttc                                      >  1:1979495/1‑98 (MQ=2)


                                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCg                                       <  1:459324/101‑1 (MQ=255)


                                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCg                                       >  1:3511728/1‑101 (MQ=255)


                                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCg                                       >  1:1447941/1‑101 (MQ=255)


                                                                ccccccccGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttca                                     <  1:2263797/99‑5 (MQ=0)


                                                                cTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttca                                     <  1:1309471/101‑5 (MQ=2)


                                                                 cccccccGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaa                                    <  2:2682562/100‑6 (MQ=0)


                                                                  ccccccGCGGGGGTGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTg                                    <  2:1047259/101‑1 (MQ=255)


                                                                  ccccccGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaat                                   <  1:1767511/101‑7 (MQ=0)


                                                                  ccccccGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaat                                   <  1:2748894/101‑7 (MQ=0)


                                                                   cccccGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGc                                   <  1:1897925/101‑1 (MQ=255)


                                                                   cccccGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGc                                   <  1:2472404/101‑1 (MQ=255)


                                                                   cccccGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGGGc                                   >  2:2952045/1‑101 (MQ=255)


                                                                    ccccGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaatgg                                 <  2:1083294/101‑9 (MQ=2)


                                                                    ccccGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaatgg                                 <  1:3321997/101‑9 (MQ=2)


                                                                      ccGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGt                                <  1:3626098/101‑1 (MQ=255)


                                                                          gggggCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGcc                            >  2:1988290/1‑100 (MQ=255)


                                                                             ggCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCCGTGCCGTCGCTCGt                         >  2:4113630/1‑101 (MQ=255)


                                                                                gTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCa                      <  2:3829519/101‑1 (MQ=255)


                                                                                gTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCa                      <  2:2359693/101‑1 (MQ=255)


                                                                                 tGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAg                     <  2:2590120/101‑1 (MQ=255)


                                                                                  ggATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGc                    >  2:1836906/1‑101 (MQ=255)


                                                                                    aTCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCg                  >  2:3624506/1‑101 (MQ=255)


                                                                                    aTCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCg                  >  2:3402862/1‑101 (MQ=255)


                                                                                      cGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCCGc                <  1:3128442/101‑1 (MQ=255)


                                                                                       gAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGcc               >  2:3085897/1‑101 (MQ=255)


                                                                                        aagcATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCt              >  1:2418477/4‑101 (MQ=255)


                                                                                        aaaCATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCt              <  2:587678/101‑1 (MQ=255)


                                                                                            aTCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtc          <  2:1626572/101‑1 (MQ=255)


                                                                                               gccg...cT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtcgtc       <  2:393811/101‑1 (MQ=255)


                                                                                                  g...cT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCt    <  2:1581909/101‑1 (MQ=255)


                                                                                                       t.ttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTcc  >  1:3211688/1‑101 (MQ=255)


                                                                                                       t.ttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTcc  >  2:1557093/1‑101 (MQ=255)


CCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCC  >  NC_014034/1395374‑1395574

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG