Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,489 G→T 19.7% intergenic (+301/‑630) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,4890G→T19.7% 126.9 ‑0.5 ‑0.0240intergenic (+301/‑630)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (18/29):  ref base (81/110):  total (99/141)
Fisher's exact test strand distribution p-value = 6.25e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.92e-01

Only 100 of 249 total aligned reads displayed.

ATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCGT  >  NC_014034/1395389‑1395588
                                                                                                        |                                                                                                   
  cGAACCTGCACCCGCTGGCCCTGCCGGTGAACGGTGATCAGCGGTCCCGCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCt                                                                                                   <  2:3503070/101‑1 (MQ=38)
  cGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCt                                                                                                   <  1:2829828/101‑1 (MQ=255)
    aaaCTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTgg                                                                                                 <  1:2509036/101‑1 (MQ=255)
      aCTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTa                                                                                               <  1:3466905/101‑1 (MQ=255)
       cTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTaa                                                                                              <  2:3003445/101‑1 (MQ=255)
        tGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAAc                                                                                             <  1:3913955/101‑1 (MQ=255)
         gCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACg                                                                                            >  2:2416645/1‑101 (MQ=255)
          cACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACgt                                                                                           >  1:1655428/1‑101 (MQ=255)
           ccccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACgtg                                                                                          <  1:3971508/100‑1 (MQ=255)
           aCCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGGAACgtg                                                                                          >  2:3057989/1‑101 (MQ=255)
            cccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACgtgt                                                                                         <  1:516525/101‑1 (MQ=255)
             ccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGTGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTc                                                                                        <  2:335861/101‑1 (MQ=255)
             ccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTc                                                                                        <  1:2726905/101‑1 (MQ=255)
               gCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGc                                                                                      >  2:3405164/1‑101 (MQ=255)
                 tGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTc                                                                                    >  2:3625845/1‑101 (MQ=255)
                  ggCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTcc                                                                                   >  1:789910/1‑101 (MQ=255)
                  ggCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTcc                                                                                   <  2:3311789/101‑1 (MQ=255)
                  ggCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CG.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTcc                                                                                   >  1:3676267/1‑101 (MQ=255)
                     ccTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTccccc                                                                                <  1:100034/101‑1 (MQ=255)
                      cTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccccc                                                                               >  2:3128211/1‑101 (MQ=255)
                      cTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccccc                                                                               <  2:52103/101‑1 (MQ=255)
                              gatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc                                                                       <  2:1922950/101‑1 (MQ=255)
                              gatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc                                                                       <  2:971995/101‑1 (MQ=255)
                                tcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGt                                                                     <  2:4177425/101‑1 (MQ=255)
                                 gggtggtgAGGGGCCGCTCCGCCCGCGGGGGCGGGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTg                                                                    <  2:1188078/100‑1 (MQ=38)
                                  ggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTgg                                                                   <  2:3912547/101‑1 (MQ=255)
                                  ggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTgg                                                                   <  1:3371570/101‑1 (MQ=255)
                                   gtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGa                                                                  >  1:107331/1‑101 (MQ=255)
                                   gctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                 <  2:2052707/92‑1 (MQ=2)
                                   gctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                 >  1:262065/10‑101 (MQ=2)
                                    tgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                 >  2:1592116/1‑101 (MQ=255)
                                    ctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                >  2:3481168/9‑101 (MQ=0)
                                     tggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                               >  1:1533640/8‑101 (MQ=2)
                                     gatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                <  2:555779/101‑1 (MQ=255)
                                      atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                               >  2:366066/1‑101 (MQ=255)
                                        cAGCGGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                             <  2:271902/101‑1 (MQ=255)
                                        acgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                            >  2:2748688/5‑101 (MQ=2)
                                         aGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                            <  1:1283247/101‑1 (MQ=255)
                                         aGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTAGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                            >  2:579190/1‑101 (MQ=255)
                                          gtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                          >  1:4108360/3‑101 (MQ=2)
                                          gCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                           <  2:3541255/101‑1 (MQ=255)
                                          gCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                           >  2:1731691/1‑101 (MQ=255)
                                          gCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                           <  2:2941449/101‑1 (MQ=255)
                                           tggTCGCTCCCCCCGCGGGGGCTTGGATCGAAACTACACCG...GG.TCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACag                                                         <  2:4226238/100‑2 (MQ=2)
                                           tggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACag                                                         >  1:2314939/2‑100 (MQ=2)
                                            ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACagc                                                        >  1:3080607/1‑99 (MQ=2)
                                            ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACagc                                                        <  2:1836845/101‑3 (MQ=2)
                                            ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                         >  1:698128/1‑101 (MQ=255)
                                            ggTCGCCCCCCCCGCGGGGGCGTGGATCAAAACTACCCCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACagc                                                        <  1:1141182/101‑3 (MQ=2)
                                              tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctt                                                      <  1:3858616/101‑2 (MQ=2)
                                              tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtct                                                       >  2:2483019/1‑101 (MQ=255)
                                               ngccncccccGCGGGGGCGTGGATCGAAAC.TCCCCGAACCTGTCGGCGG.CGA.ACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtcg                                                       <  1:3196898/96‑2 (MQ=0)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgcttt                                                     <  1:41447/101‑3 (MQ=2)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtctc                                                      <  1:5830/101‑1 (MQ=255)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttc                                                    <  1:993780/101‑4 (MQ=2)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCg                                                     >  1:831056/1‑101 (MQ=255)
                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGTCGTGGATCGAAACAgctttca                                                   <  1:2399969/101‑5 (MQ=2)
                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttca                                                   <  1:1471450/101‑5 (MQ=2)
                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttca                                                   <  1:1309471/101‑5 (MQ=2)
                                                   ccccccGCGGGGGTGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTg                                                  <  2:1047259/101‑1 (MQ=255)
                                                   ccccccGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaat                                                 <  1:1767511/101‑7 (MQ=0)
                                                   ccccccGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaat                                                 >  2:145188/1‑95 (MQ=0)
                                                   ccccccGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaat                                                 <  2:2921057/101‑7 (MQ=0)
                                                    cccccGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGc                                                 <  1:1897925/101‑1 (MQ=255)
                                                     ccccGCGGGGGCGTGGATCGAAACTACACCG...GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaatgg                                               <  1:3321997/101‑9 (MQ=2)
                                                       ccGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGt                                              <  1:3626098/101‑1 (MQ=255)
                                                        cgcgGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTc                                             >  1:554238/1‑101 (MQ=255)
                                                          cgGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGc                                           <  1:158392/101‑1 (MQ=255)
                                                              ggCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGt                                       <  2:2075301/101‑1 (MQ=255)
                                                              ggCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCCGTGCCGTCGCTCGt                                       >  2:4113630/1‑101 (MQ=255)
                                                              ggCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCTCGGTGCCGTCGCTCgc                                       >  1:422710/1‑100 (MQ=255)
                                                                cGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTTGATCGAAACATCTCGGTGCCGTCGCTCGTcc                                     <  1:3754861/101‑1 (MQ=255)
                                                                cGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTcc                                     <  1:1947437/101‑1 (MQ=255)
                                                                 gTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCa                                    <  2:2359693/101‑1 (MQ=255)
                                                                 gTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCa                                    <  2:3829519/101‑1 (MQ=255)
                                                                  tGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAg                                   <  2:2590120/101‑1 (MQ=255)
                                                                     aTCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCg                                <  2:1655313/101‑1 (MQ=255)
                                                                       cGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGc                              <  1:204577/101‑1 (MQ=255)
                                                                       cGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCCGc                              <  1:3128442/101‑1 (MQ=255)
                                                                        atctcATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGcc                             <  2:912609/97‑1 (MQ=255)
                                                                          aaCATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTg                           <  1:2257267/101‑1 (MQ=255)
                                                                           aCATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGc                          >  2:630643/1‑101 (MQ=255)
                                                                            cATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCt                         >  2:2007715/1‑101 (MQ=255)
                                                                              tCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtcg                       <  1:2916134/101‑1 (MQ=255)
                                                                               cgccg...cT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtcgt                      >  1:2983715/1‑101 (MQ=255)
                                                                                gccg...cT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtcgtc                     <  2:393811/101‑1 (MQ=255)
                                                                                   g...cT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCt                  <  2:1581909/101‑1 (MQ=255)
                                                                                        t.ttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTcc                >  1:3211688/1‑101 (MQ=255)
                                                                                        t.ttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTcc                >  2:1557093/1‑101 (MQ=255)
                                                                                           tGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTcccc              >  1:2098719/1‑101 (MQ=255)
                                                                                            ggCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTccccc             >  1:4171262/1‑101 (MQ=255)
                                                                                             gCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTcccccc            <  2:1162012/101‑1 (MQ=255)
                                                                                              cGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCcg           >  1:3543589/1‑101 (MQ=255)
                                                                                               ggTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCcgc          >  2:4149808/1‑101 (MQ=255)
                                                                                                gTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCcccg         <  1:2416821/101‑1 (MQ=255)
                                                                                                  cGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGGTCGTCGCTTCCCCCGCggg       >  2:3205056/1‑101 (MQ=255)
                                                                                                  cGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCggg       >  2:765501/1‑101 (MQ=255)
                                                                                                   gACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCgggg      <  2:2151676/101‑1 (MQ=255)
                                                                                                     cAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGc    >  2:1083280/1‑101 (MQ=255)
                                                                                                     cAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGc    >  1:1794387/1‑101 (MQ=255)
                                                                                                        |                                                                                                   
ATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG...CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCGT  >  NC_014034/1395389‑1395588

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG