Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,545 C→A 16.2% intergenic (+357/‑574) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,5450C→A16.2% 125.1 ‑1.8 ‑0.2303intergenic (+357/‑574)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (29/20):  ref base (118/132):  total (147/156)
Fisher's exact test strand distribution p-value = 1.59e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.58e-01

Only 100 of 322 total aligned reads displayed.

CGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCG  >  NC_014034/1395445‑1395645
                                                                                                    |                                                                                                      
  cgGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGc                                                                                                      <  1:158392/101‑1 (MQ=255)
   gggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGcc                                                                                                     >  2:1988290/1‑100 (MQ=255)
    ggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGGCACTc                                                                                                    >  2:1153172/1‑101 (MQ=255)
      ggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.t                                                                                                 <  2:2075301/101‑1 (MQ=255)
       gCGTTGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCTCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.Tc                                                                                                <  2:554174/101‑1 (MQ=255)
        cGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTTGATCGAAACATCTCGGTGCCGTCGCTCG.Tcc                                                                                               <  1:3754861/101‑1 (MQ=255)
         gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCa                                                                                              <  2:3829519/101‑1 (MQ=255)
         gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCa                                                                                              >  1:1936922/1‑101 (MQ=255)
             aTCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCg                                                                                          >  2:3402862/1‑101 (MQ=255)
                gAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.cc                                                                                      >  2:3085897/1‑101 (MQ=255)
                     aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCtc                                                                                 >  1:178262/1‑101 (MQ=255)
                     aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCtc                                                                                 <  2:1626572/101‑1 (MQ=255)
                     aTCGCCGCTTTGGCGGTCGACAGCCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCtc                                                                                 <  2:544165/101‑1 (MQ=255)
                            cTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTc                                                                          <  1:3725765/101‑1 (MQ=255)
                             tttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTcc                                                                         >  2:1557093/1‑101 (MQ=255)
                               tGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTcccc                                                                       >  1:2098719/1‑101 (MQ=255)
                                ggCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTccccc                                                                      >  1:4171262/1‑101 (MQ=255)
                                   ggTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCcgc                                                                   >  1:2643189/1‑101 (MQ=255)
                                    gTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCcccg                                                                  <  1:2416821/101‑1 (MQ=255)
                                       gACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCgggg                                                               <  1:2018102/101‑1 (MQ=255)
                                       gACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCgggg                                                               <  2:3638415/101‑1 (MQ=255)
                                         cAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGc                                                             >  2:2403158/1‑101 (MQ=255)
                                         cAGGCTGGTAACGTGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGc                                                             <  1:579254/101‑1 (MQ=255)
                                             agcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTgg                                                         <  2:2285179/91‑1 (MQ=0)
                                             agcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTgg                                                         >  2:1021061/11‑101 (MQ=0)
                                             aaggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTgg                                                         >  2:459927/11‑101 (MQ=2)
                                             aaggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTgg                                                         <  1:2395909/91‑1 (MQ=2)
                                             aaggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTgg                                                         >  1:1035369/11‑101 (MQ=2)
                                              aggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGa                                                        >  1:3435224/10‑101 (MQ=0)
                                               ggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGAt                                                       >  1:3540112/9‑101 (MQ=2)
                                               ggTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                       >  1:391596/1‑101 (MQ=255)
                                               ggTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGCCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCggggggggggca                                                       >  2:2793921/1‑94 (MQ=255)
                                               cctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                       <  2:404372/93‑1 (MQ=2)
                                                 tAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                     >  1:440169/1‑101 (MQ=255)
                                                  aaCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                    >  1:1096869/1‑101 (MQ=255)
                                                  aaCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGGGGATCGa                                                    >  1:1819046/1‑101 (MQ=255)
                                                   gcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                   >  1:1743777/5‑101 (MQ=2)
                                                    gtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                  >  2:2618523/4‑101 (MQ=2)
                                                    gtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                  <  2:1356493/98‑1 (MQ=2)
                                                    gcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                  <  1:2176752/98‑1 (MQ=2)
                                                    ctggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGACGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                  >  2:3982834/4‑101 (MQ=255)
                                                    cacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                  <  2:26960/98‑1 (MQ=2)
                                                     tggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                 >  1:3003947/3‑101 (MQ=2)
                                                     acgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGCTCGAAac                                                 >  2:721106/3‑101 (MQ=2)
                                                      ggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                                <  2:961627/100‑1 (MQ=2)
                                                      ggtCGCCCCCCCCGCGGGGGCGTGGATCGAAACAAAAACCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                                <  1:1378143/100‑1 (MQ=2)
                                                       gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacta                                               <  2:3126514/101‑3 (MQ=2)
                                                        tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccac                                              <  1:962466/101‑1 (MQ=255)
                                                        tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacccc                                              <  1:360973/101‑3 (MQ=2)
                                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccg                                             >  2:2358172/1‑98 (MQ=2)
                                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccg                                             >  1:543445/1‑98 (MQ=2)
                                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCt                                            >  2:3322624/1‑101 (MQ=255)
                                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCt                                            >  2:1827376/1‑101 (MQ=255)
                                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgc                                            >  2:899049/1‑97 (MQ=2)
                                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgc                                            >  1:4004352/1‑96 (MQ=2)
                                                           cTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCtt                                           <  2:107307/101‑1 (MQ=255)
                                                           cTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCtt                                           <  2:4126783/101‑1 (MQ=255)
                                                           cTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCtt                                           >  1:3155118/1‑101 (MQ=255)
                                                           cTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCtt                                           >  1:323421/1‑101 (MQ=255)
                                                           cTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgct                                           >  1:3318772/1‑95 (MQ=2)
                                                           cTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgct                                           <  2:1970063/101‑7 (MQ=2)
                                                            tCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTg                                          >  1:2974060/1‑101 (MQ=255)
                                                            tCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAacaccgcgg                                          >  2:3193246/1‑94 (MQ=2)
                                                            tCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctg                                          <  1:1225729/101‑8 (MQ=2)
                                                             ccccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGc                                         <  2:2478634/101‑1 (MQ=255)
                                                             ccccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGc                                         >  2:2131950/1‑101 (MQ=255)
                                                             ccccccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgcg                                         <  2:1795645/101‑9 (MQ=2)
                                                             ccccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggc                                         >  2:3931076/1‑94 (MQ=2)
                                                             ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                         >  2:2425070/1‑93 (MQ=2)
                                                             ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                         >  1:3345775/1‑93 (MQ=2)
                                                              cccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctggc                                        <  1:277709/101‑10 (MQ=2)
                                                               ccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggcgt                                       <  1:1961815/101‑10 (MQ=2)
                                                               ccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggcgg                                       >  2:580161/1‑92 (MQ=2)
                                                                 ccGCGGGGGCGTGGATCGAAACATCTCGGCGCCGTCGCTCG.TCCCGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAt                                     <  1:2483203/101‑1 (MQ=255)
                                                                  cgcgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAtt                                    <  2:3601743/101‑1 (MQ=255)
                                                                  cgcgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAtt                                    <  2:3211449/101‑1 (MQ=255)
                                                                   gcgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTg                                   >  2:1691174/1‑101 (MQ=255)
                                                                    cgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGt                                  <  2:1447839/101‑1 (MQ=255)
                                                                     gggggCGTGGGTCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCCTTGCGGATTGTc                                 <  2:1763114/101‑1 (MQ=255)
                                                                     gggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTc                                 >  2:1310057/1‑101 (MQ=255)
                                                                       gggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCAc                               <  1:2726450/101‑1 (MQ=255)
                                                                       gggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCAc                               >  2:3496251/1‑101 (MQ=255)
                                                                       gggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCAc                               <  1:3033485/101‑1 (MQ=255)
                                                                          cGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACttt                            >  1:1354653/1‑101 (MQ=255)
                                                                           gTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                           <  1:117110/101‑1 (MQ=255)
                                                                           ctGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                           <  2:3017343/100‑1 (MQ=255)
                                                                            tGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcg                          <  1:3086127/101‑1 (MQ=255)
                                                                            tGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcg                          <  2:4171000/101‑1 (MQ=255)
                                                                                tCGAAGCATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCggg                      <  2:388774/101‑1 (MQ=255)
                                                                                tCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCggg                      <  2:830975/101‑1 (MQ=255)
                                                                                  gAAACATCTCGGTGCCGTCGCTCG.TCAAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGc                    >  2:627097/1‑101 (MQ=255)
                                                                                      cATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAg                >  1:2890949/1‑101 (MQ=255)
                                                                                        tctcGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCg              >  1:1151060/1‑101 (MQ=255)
                                                                                        tctcGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCg              >  2:2552311/1‑101 (MQ=255)
                                                                                            ggTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGc          >  1:660492/1‑101 (MQ=255)
                                                                                              tGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTc        >  2:344238/1‑101 (MQ=255)
                                                                                               gCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcc       >  1:3389267/1‑101 (MQ=255)
                                                                                                  gTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTccccc    >  1:877272/1‑101 (MQ=255)
                                                                                                   tCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcccccc   <  1:3813103/101‑1 (MQ=255)
                                                                                                   tCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcccccc   <  1:3678315/101‑1 (MQ=255)
                                                                                                    |                                                                                                      
CGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCG  >  NC_014034/1395445‑1395645

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG