Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,666 T→C 6.1% intergenic (+478/‑453) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,6660T→C 6.1% 17.2 ‑0.6 ‑0.1181intergenic (+478/‑453)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/4):  ref base (86/84):  total (93/88)
Fisher's exact test strand distribution p-value = 5.38e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.91e-01

Only 100 of 204 total aligned reads displayed.

CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTC  >  NC_014034/1395566‑1395766
                                                                                                    |                                                                                                        
cgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                                                                          >  2:3342271/1‑101 (MQ=255)
cgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                                                                          <  2:3543336/101‑1 (MQ=255)
cgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                                                                          >  1:2609573/1‑101 (MQ=255)
 gtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTc                                                                                                         >  2:3572247/1‑101 (MQ=255)
 gtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                                                                         >  1:2154496/1‑101 (MQ=255)
      tCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCAt                                                                                                    <  2:660415/101‑1 (MQ=255)
       ccccccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATg                                                                                                   <  1:1619279/101‑1 (MQ=255)
        cccccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGt                                                                                                  <  2:1799200/101‑1 (MQ=255)
         ccccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTa                                                                                                 <  2:1890866/101‑1 (MQ=255)
          cccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAg                                                                                                >  1:2741502/1‑101 (MQ=255)
           ccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGt                                                                                               <  2:789832/101‑1 (MQ=255)
            cgcgGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTc                                                                                              >  2:2382383/1‑101 (MQ=255)
              cgGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcgg                                                                                            >  1:960583/1‑101 (MQ=255)
              cgGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcgg                                                                                            >  2:511482/1‑101 (MQ=255)
               gggggCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcggc                                                                                           <  1:1941426/101‑1 (MQ=255)
               gggggCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcggc                                                                                           >  1:787532/1‑101 (MQ=255)
                  ggCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCg..g.g                                                                                     <  1:2507234/101‑1 (MQ=255)
                    cGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCa                                                                                   <  2:1150966/101‑1 (MQ=255)
                    cGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCa                                                                                   <  2:2090907/101‑1 (MQ=255)
                    cGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCa                                                                                   <  1:630707/101‑1 (MQ=255)
                      tGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGt                                                                                 <  2:2142036/101‑1 (MQ=255)
                      tGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGt                                                                                 <  2:2642983/101‑1 (MQ=255)
                       ggATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGtt                                                                                >  2:4113940/1‑101 (MQ=255)
                       ggATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGtt                                                                                >  1:890488/1‑101 (MQ=255)
                        gATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTc                                                                               <  1:60362/101‑1 (MQ=255)
                          tCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGa                                                                             <  1:1083374/101‑1 (MQ=255)
                          tCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGa                                                                             <  1:548626/101‑1 (MQ=255)
                            gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.Gc                                                                          >  1:1876126/1‑101 (MQ=255)
                                cccccTTGCGGATTGTCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCa                                                                      <  1:3983094/98‑1 (MQ=255)
                                ccaccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCa                                                                      >  2:3412179/1‑101 (MQ=255)
                                  accTTGCGGATTGTCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGt                                                                    <  2:440112/101‑1 (MQ=255)
                                   ccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTc                                                                   <  1:1691289/101‑1 (MQ=255)
                                   ccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTc                                                                   >  1:479137/1‑101 (MQ=255)
                                      ggCGGATTGCCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCt                                                                <  2:3358922/100‑1 (MQ=255)
                                       gCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTc                                                               >  2:3185451/1‑101 (MQ=255)
                                          gATTGTCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTcccc                                                            <  1:664524/101‑1 (MQ=255)
                                          gATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTcccc                                                            <  2:174846/101‑1 (MQ=255)
                                           aTTGTCACTTTCGCGGGGCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTccccc                                                           <  2:3389022/101‑1 (MQ=255)
                                            ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTcccccc                                                          <  1:1592225/101‑1 (MQ=255)
                                            ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTcccccc                                                          <  1:4150070/101‑1 (MQ=255)
                                                   tttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCggggg                                                   >  1:2091141/1‑101 (MQ=255)
                                                   tttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCggggg                                                   >  2:429380/1‑101 (MQ=255)
                                                    ttCGCGGGGCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGc                                                  <  2:48514/101‑1 (MQ=255)
                                                       gcgGGCCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTg                                               <  2:2611230/101‑1 (MQ=255)
                                                        ggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTg                                               >  1:3257903/10‑101 (MQ=2)
                                                        ggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTg                                               >  2:3429367/10‑101 (MQ=2)
                                                         ggggCGAAGCGTCGCTCCCCCGGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGa                                             >  1:3114965/1‑101 (MQ=255)
                                                         ggggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGa                                             >  2:3654634/1‑101 (MQ=255)
                                                         ggggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGa                                             >  1:2549836/1‑101 (MQ=255)
                                                         ggggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGa                                             <  1:348264/101‑1 (MQ=255)
                                                         gatcccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCCTGACCGCATCGA.A.GCTGGTCAC.ATCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                              <  2:2197809/91‑1 (MQ=2)
                                                           ggCGAAGCGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGGATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATc                                           <  1:2794140/101‑1 (MQ=255)
                                                           gccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                            <  1:3901565/95‑1 (MQ=2)
                                                           gccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                            >  2:1021792/7‑101 (MQ=2)
                                                           gccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                            <  2:2750288/95‑1 (MQ=2)
                                                              gAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                        >  1:1270206/1‑101 (MQ=255)
                                                              gAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                        >  2:22398/1‑101 (MQ=255)
                                                               gtgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCGGCAGTGTCA.GGTTTTCCA.GCGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                        <  1:3235548/99‑1 (MQ=2)
                                                                aGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                      >  1:4028785/1‑101 (MQ=255)
                                                                  cggcGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                    <  1:3325631/98‑1 (MQ=255)
                                                                  cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                    >  1:14741/1‑101 (MQ=255)
                                                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACTgga                                  >  1:1031621/1‑100 (MQ=255)
                                                                    ccGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGt                                  <  1:1816451/100‑1 (MQ=255)
                                                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTa                                 >  1:3056867/1‑101 (MQ=255)
                                                                     cGCCCCCCCCGGGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTa                                 <  1:2922167/101‑1 (MQ=255)
                                                                       cttcccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGGAACTGGTATg                               >  2:875290/4‑101 (MQ=255)
                                                                        tCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                              >  1:2966343/1‑101 (MQ=255)
                                                                        tCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                              >  2:1172760/1‑101 (MQ=255)
                                                                        tCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                              >  2:1603454/1‑101 (MQ=255)
                                                                        cccccccGGGGGGGGGGGGATCAAAACACCTGCTTGTAGTGGGCG..G.GCGGTCGGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                              <  1:3873470/100‑1 (MQ=21)
                                                                            cccGCGGGGGGGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCt                          <  1:2698946/101‑1 (MQ=255)
                                                                            cccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCt                          <  1:4199048/101‑1 (MQ=255)
                                                                            cccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCt                          <  2:3153209/101‑1 (MQ=255)
                                                                            cccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCt                          <  1:3439648/101‑1 (MQ=255)
                                                                              cgcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTgg                        <  2:2499199/101‑1 (MQ=255)
                                                                              cgcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTgg                        >  1:2612570/1‑101 (MQ=255)
                                                                               gcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGGTCTGgc                       >  1:594519/1‑101 (MQ=255)
                                                                               gcgGGGGCGTGGACCGAAACATCGGCGGGGAGTCGGCG..G.GCAGTTGGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                       <  2:4017738/101‑1 (MQ=37)
                                                                                 gggggCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCTCAGTCGATTCCCCCGCGGGGGCGTGGATCGAAACTGTTATGGATGTgactc                     >  2:2072258/1‑97 (MQ=37)
                                                                                   gggCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCaa                   >  2:2959342/1‑101 (MQ=255)
                                                                                      cGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACg                >  2:3742661/1‑101 (MQ=255)
                                                                                      cGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACg                >  1:1783396/1‑101 (MQ=255)
                                                                                       gTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACgg               <  1:3185690/101‑1 (MQ=255)
                                                                                       gTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACgg               >  1:640301/1‑101 (MQ=255)
                                                                                        tGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa              >  1:3518232/1‑101 (MQ=255)
                                                                                        gggATCGAACCATCTGCACGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa              <  2:1913152/100‑1 (MQ=255)
                                                                                         gggtCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa             >  1:2416128/4‑101 (MQ=255)
                                                                                         ggATCGAAACATCTGCAGGTGGTCGGCG..G.GCAGTGCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa             <  2:1818926/101‑1 (MQ=255)
                                                                                          gATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaaa            >  2:616699/1‑101 (MQ=255)
                                                                                          gATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAATGGTATCGGTCTGGCGCAAACGGaag            >  2:3092418/1‑100 (MQ=255)
                                                                                             cGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTc         >  2:554227/1‑101 (MQ=255)
                                                                                             cGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCCTGGATCGAAACTGGTATGGATCTGGCGTGAACGGAAAGTc         >  1:1398615/1‑101 (MQ=255)
                                                                                              gAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt        >  1:2364363/1‑101 (MQ=255)
                                                                                              gAAACACCGGCAGGTAGGCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt        <  2:3901031/101‑1 (MQ=37)
                                                                                                aaCATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgt      <  2:1270110/101‑1 (MQ=255)
                                                                                                  cATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGCCTgtcg    >  2:3220016/1‑101 (MQ=255)
                                                                                                   aTCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgt   <  2:591493/101‑1 (MQ=255)
                                                                                                   aTCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgt   >  1:3550556/1‑101 (MQ=255)
                                                                                                    tCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgtc  >  2:1643354/1‑101 (MQ=255)
                                                                                                    tCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgtc  >  2:3507926/1‑101 (MQ=255)
                                                                                                    |                                                                                                        
CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTC  >  NC_014034/1395566‑1395766

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG