BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,395,670	C→G	6.4%	intergenic (+482/‑449)	RCAP_rcc01306 → / → RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,395,670	0	C→G	6.4%	17.9 ‑0.3 ‑0.1	181	intergenic (+482/‑449)	RCAP_rcc01306/RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (7/5): ref base (89/79): total (96/85)
Fisher's exact test strand distribution p-value = 7.73e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.16e-01

Only 100 of 201 total aligned reads displayed.


GCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTC  >  NC_014034/1395570‑1395770


gCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGc                                                                                                           >  1:1913278/1‑101 (MQ=255)


gCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCCCGGGGGCGTGGATCGAAACATCTGc                                                                                                           >  1:3055888/1‑101 (MQ=255)


  tCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCAt                                                                                                         <  2:660415/101‑1 (MQ=255)


    cccccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGt                                                                                                       <  2:1799200/101‑1 (MQ=255)


       ccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGt                                                                                                    <  2:789832/101‑1 (MQ=255)


        cgcgGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTc                                                                                                   >  2:2382383/1‑101 (MQ=255)


          cgGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcgg                                                                                                 >  2:511482/1‑101 (MQ=255)


           gggggCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcgg.c                                                                                               >  1:787532/1‑101 (MQ=255)


           gggggCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcgg.c                                                                                               >  1:1613343/1‑101 (MQ=255)


              ggCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.Cg.g.g                                                                                          <  1:2507234/101‑1 (MQ=255)


               gCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.Gc                                                                                         >  1:3153448/1‑101 (MQ=255)


                cGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCa                                                                                        <  2:2090907/101‑1 (MQ=255)


                cGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCa                                                                                        <  1:630707/101‑1 (MQ=255)


                  tGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGt                                                                                      <  2:2142036/101‑1 (MQ=255)


                  tGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGt                                                                                      <  2:2642983/101‑1 (MQ=255)


                   ggATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGtt                                                                                     >  1:890488/1‑101 (MQ=255)


                   ggATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGtt                                                                                     >  2:4113940/1‑101 (MQ=255)


                    gATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTc                                                                                    <  1:60362/101‑1 (MQ=255)


                      tCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGa                                                                                  >  2:3549874/1‑101 (MQ=255)


                      tCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGa                                                                                  <  1:1083374/101‑1 (MQ=255)


                      tCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGa                                                                                  <  1:548626/101‑1 (MQ=255)


                        gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.Gc                                                                               >  2:1430809/1‑101 (MQ=255)


                        gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.Gc                                                                               >  1:2611421/1‑101 (MQ=255)


                          aaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCg                                                                             >  1:2944103/1‑101 (MQ=255)


                            cccccTTGCGGATTGTCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.Ca                                                                          <  1:3983094/98‑1 (MQ=255)


                            ccaccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.Ca                                                                          >  2:3412179/1‑101 (MQ=255)


                              accTTGCGGATTGTCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGt                                                                        <  2:440112/101‑1 (MQ=255)


                               ccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTc                                                                       >  1:479137/1‑101 (MQ=255)


                               ccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTc                                                                       <  1:1691289/101‑1 (MQ=255)


                                  ggCGGATTGCCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCt                                                                    <  2:3358922/100‑1 (MQ=255)


                                    cGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTcc                                                                  >  1:1327968/1‑101 (MQ=255)


                                      gATTGTCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTcccc                                                                <  1:664524/101‑1 (MQ=255)


                                      gATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTcccc                                                                <  2:174846/101‑1 (MQ=255)


                                      gATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTcccc                                                                <  1:1557197/101‑1 (MQ=255)


                                       aTTGTCACTTTCGCGGGGCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTccccc                                                               <  2:3389022/101‑1 (MQ=255)


                                        ttGTCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTcccccc                                                              <  1:2132095/101‑1 (MQ=255)


                                        ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTcccccc                                                              <  1:4150070/101‑1 (MQ=255)


                                        ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTcccccc                                                              >  2:1159018/1‑101 (MQ=255)


                                               tttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCggggg                                                       >  2:429380/1‑101 (MQ=255)


                                                ttCGCGGGGCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGc                                                      <  2:48514/101‑1 (MQ=255)


                                                   gcgGGCCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTg                                                   <  2:2611230/101‑1 (MQ=255)


                                                    ggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG.GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTg                                                   >  2:3429367/10‑101 (MQ=2)


                                                    ggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG.GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTg                                                   >  1:3257903/10‑101 (MQ=2)


                                                    ggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG.GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTg                                                   >  1:2920285/10‑101 (MQ=2)


                                                     ggggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGa                                                 <  1:348264/101‑1 (MQ=255)


                                                     ggggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGa                                                 >  2:3654634/1‑101 (MQ=255)


                                                     ggggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGa                                                 >  1:2549836/1‑101 (MQ=255)


                                                     gatcccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCCTGACCGCAT.CGAA.GCTGGTCAC.ATCG.CCGTCGCTCCCCCCGCGGGGGCGTgg                                                  <  2:2197809/91‑1 (MQ=2)


                                                       gccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG.GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                <  2:2750288/95‑1 (MQ=2)


                                                       gccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG.GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                <  1:3901565/95‑1 (MQ=2)


                                                          gAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                            >  2:22398/1‑101 (MQ=255)


                                                           catcGTCGCTCCCCCCGCGGGGACGTGGATCGAATCCTATGAGGGCTGGCGGCCC.G.GTCGTTGAT.CCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                             <  2:3165394/98‑1 (MQ=0)


                                                           aaGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGaac                                           >  2:1892291/1‑100 (MQ=255)


                                                            aGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                          >  1:4028785/1‑101 (MQ=255)


                                                             gCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                         >  1:2687011/1‑101 (MQ=255)


                                                              cggcGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                        <  1:3325631/98‑1 (MQ=255)


                                                              cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG.GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaaaa                                         >  2:3298710/1‑99 (MQ=2)


                                                                tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACTgga                                      >  1:1031621/1‑100 (MQ=255)


                                                                tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGt                                      <  1:2778662/101‑1 (MQ=255)


                                                                ccGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGt                                      <  1:1816451/100‑1 (MQ=255)


                                                                   cttcccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGGAACTGGTATg                                   >  2:875290/4‑101 (MQ=255)


                                                                    tCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                  >  1:2966343/1‑101 (MQ=255)


                                                                    tCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                  >  2:1603454/1‑101 (MQ=255)


                                                                    cccccccGGGGGGGGGGGGATCAAAACACCTGCTTGTAGTGGG.CG.G.GCGGTCGGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                  <  1:3873470/100‑1 (MQ=21)


                                                                        cccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCt                              <  1:3439648/101‑1 (MQ=255)


                                                                        cccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCt                              <  1:4199048/101‑1 (MQ=255)


                                                                        cccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCt                              <  2:3153209/101‑1 (MQ=255)


                                                                          cgcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTgg                            <  2:2499199/101‑1 (MQ=255)


                                                                           ggggggcCGTGAATCGAAACATCTGGTTGTGGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                           <  2:1048267/99‑1 (MQ=37)


                                                                           gcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGGTCTGgc                           >  1:594519/1‑101 (MQ=255)


                                                                           gcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                           >  1:1403156/1‑101 (MQ=255)


                                                                           gcgGGGGCGTGGACCGAAACATCGGCGGGGAGTCGG.CG.G.GCAGTTGGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                           <  2:4017738/101‑1 (MQ=37)


                                                                             gggggCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCT.CAGTCGATTCCCCCGCGGGGGCGTGGATCGAAACTGTTATGGATGTgactc                         >  2:2072258/1‑97 (MQ=37)


                                                                             gggggCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgcgc                         >  2:3200016/1‑101 (MQ=255)


                                                                               gggCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCaa                       >  2:2959342/1‑101 (MQ=255)


                                                                                 gCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAAc                     >  1:2737661/1‑101 (MQ=255)


                                                                                  cGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACg                    >  1:1783396/1‑101 (MQ=255)


                                                                                  cGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACg                    >  2:3742661/1‑101 (MQ=255)


                                                                                   gTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACgg                   >  1:640301/1‑101 (MQ=255)


                                                                                    tGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa                  >  1:3518232/1‑101 (MQ=255)


                                                                                     gggtCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa                 >  1:2416128/4‑101 (MQ=255)


                                                                                     ggATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa                 <  1:3194024/101‑1 (MQ=255)


                                                                                     ggATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa                 <  1:3069006/101‑1 (MQ=255)


                                                                                     ggATCGAAACATCTGCAGGTGGTCGG.CG.G.GCAGTGCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa                 <  2:1818926/101‑1 (MQ=255)


                                                                                      gATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaaa                >  2:1187649/1‑101 (MQ=255)


                                                                                      gATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaaa                >  2:616699/1‑101 (MQ=255)


                                                                                      gATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAATGGTATCGGTCTGGCGCAAACGGaag                >  2:3092418/1‑100 (MQ=255)


                                                                                         cGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTc             >  2:554227/1‑101 (MQ=255)


                                                                                         cGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTc             >  1:964882/1‑101 (MQ=255)


                                                                                          gAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt            >  1:2364363/1‑101 (MQ=255)


                                                                                          gAAACACCGGCAGGTAGGCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt            <  2:3901031/101‑1 (MQ=37)


                                                                                            aaCATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgt          <  1:2043358/101‑1 (MQ=255)


                                                                                               aTCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgt       <  2:591493/101‑1 (MQ=255)


                                                                                               aTCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgt       >  1:3550556/1‑101 (MQ=255)


                                                                                                tCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgtc      >  2:3507926/1‑101 (MQ=255)


                                                                                                tCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgtc      >  2:1643354/1‑101 (MQ=255)


                                                                                                tCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTggcgtc      >  1:919872/1‑101 (MQ=255)


                                                                                                 cTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGtcgtcg     <  1:4113891/101‑1 (MQ=255)


                                                                                                   gCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCt   >  2:1925088/1‑101 (MQ=255)


                                                                                                   gCATGTAGCCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCt   <  2:1876006/101‑1 (MQ=255)


GCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG.G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTC  >  NC_014034/1395570‑1395770

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 25 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG