Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,673 G→C 6.1% intergenic (+485/‑446) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,6730G→C 6.1% 19.3 ‑0.6 ‑0.2181intergenic (+485/‑446)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/4):  ref base (91/79):  total (98/83)
Fisher's exact test strand distribution p-value = 5.52e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.71e-01

Only 100 of 205 total aligned reads displayed.

CCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCC  >  NC_014034/1395573‑1395773
                                                                                                    |                                                                                                        
ccccccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATg                                                                                                          <  1:1619279/101‑1 (MQ=255)
ccccccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATg                                                                                                          <  2:3511475/101‑1 (MQ=255)
 cccccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGt                                                                                                         <  2:1799200/101‑1 (MQ=255)
  ccccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTa                                                                                                        <  2:1890866/101‑1 (MQ=255)
    ccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGt                                                                                                      <  2:789832/101‑1 (MQ=255)
      gcgGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcg                                                                                                    <  2:2098581/101‑1 (MQ=255)
       cgGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcgg                                                                                                   <  2:486335/101‑1 (MQ=255)
       cgGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcgg                                                                                                   >  1:960583/1‑101 (MQ=255)
        gggggCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcgg.c                                                                                                 >  1:1787378/1‑101 (MQ=255)
        gggggCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcgg.c                                                                                                 <  1:1941426/101‑1 (MQ=255)
        gggggCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcgg.c                                                                                                 <  1:661712/101‑1 (MQ=255)
           ggCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.Cgg.g                                                                                             <  1:2507234/101‑1 (MQ=255)
            gCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.Gc                                                                                            >  1:3153448/1‑101 (MQ=255)
             cGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCa                                                                                           <  1:627161/101‑1 (MQ=255)
               tGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGt                                                                                         >  2:443055/1‑101 (MQ=255)
                   tCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGa                                                                                     <  1:4022781/101‑1 (MQ=255)
                   tCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGa                                                                                     <  1:1083374/101‑1 (MQ=255)
                    cGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.g                                                                                   <  1:546559/101‑1 (MQ=255)
                     gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGC.Gc                                                                                  >  2:3439396/1‑101 (MQ=255)
                     gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.Gc                                                                                  <  2:1936796/101‑1 (MQ=255)
                     gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.Gc                                                                                  >  1:2611421/1‑101 (MQ=255)
                      aaaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.Gcc                                                                                 >  1:3930954/1‑101 (MQ=255)
                      aaaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.Gcc                                                                                 >  1:636931/1‑101 (MQ=255)
                       aaCCACCTTGCGGATTGTAACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCg                                                                                >  2:524881/1‑101 (MQ=255)
                           accTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAgc                                                                           >  2:4198786/1‑100 (MQ=255)
                               tGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCt                                                                       <  1:1699823/101‑1 (MQ=255)
                               tGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCt                                                                       >  1:893133/1‑101 (MQ=255)
                                ggggATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTc                                                                      <  2:660294/99‑1 (MQ=255)
                                 cGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTcc                                                                     >  1:1327968/1‑101 (MQ=255)
                                 cGGATTCTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCATCTGCATGTAGTCGG.CGG.GCAGTTCGC.GCCG.CAGTCGCTcc                                                                     >  2:578748/1‑101 (MQ=255)
                                   gATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTcccc                                                                   <  2:174846/101‑1 (MQ=255)
                                    aTTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTccccc                                                                  >  1:591557/1‑101 (MQ=255)
                                     ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTcccccc                                                                 <  2:2154351/101‑1 (MQ=255)
                                     ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTcccccc                                                                 <  2:3398002/101‑1 (MQ=255)
                                     ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTcccccc                                                                 <  1:1592225/101‑1 (MQ=255)
                                     ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTcccccc                                                                 >  2:1159018/1‑101 (MQ=255)
                                      tGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCcg                                                                >  2:2614954/1‑101 (MQ=255)
                                            tttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCggggg                                                          >  1:2091141/1‑101 (MQ=255)
                                             ttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGc                                                         <  2:3874758/101‑1 (MQ=255)
                                                 ggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CGGCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTg                                                      >  2:3624233/10‑101 (MQ=2)
                                                 ggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CGGCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTg                                                      >  1:3257903/10‑101 (MQ=2)
                                                 gggggCGAGGCGGCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTgg                                                     <  2:1832047/100‑1 (MQ=255)
                                                 gggggCGAAGCGTCCCCCCCCCCGCGGGGGCGGGGATCGAAACATCTGCATGTGGTCGG.CGG.GGAGTTGGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTgg                                                     <  1:3893157/100‑1 (MQ=38)
                                                 cgGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTgg                                                     >  2:599265/1‑101 (MQ=255)
                                                  ggggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGa                                                    >  1:2549836/1‑101 (MQ=255)
                                                  ggggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGa                                                    <  1:348264/101‑1 (MQ=255)
                                                   gggCGAAGCGTCGCCCCCCCCGGGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                   <  1:765578/101‑1 (MQ=255)
                                                    ggCGAAGCGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGGATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                  <  1:2794140/101‑1 (MQ=255)
                                                    gccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CGGCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                   <  2:2750288/95‑1 (MQ=2)
                                                     ccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CGGCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                  <  2:3387133/96‑1 (MQ=2)
                                                        ctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CGGCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                               <  1:4104645/99‑1 (MQ=2)
                                                        catcGTCGCTCCCCCCGCGGGGACGTGGATCGAATCCTATGAGGGCTGGCGGCCCG.GTCGTTGAT.CCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                <  2:3165394/98‑1 (MQ=0)
                                                         tgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CGGCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                              >  1:2362919/2‑101 (MQ=2)
                                                          gCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                            >  1:2687011/1‑101 (MQ=255)
                                                           ggccGCGCCCCCGGGGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                           <  1:598615/98‑1 (MQ=37)
                                                           cggcGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                           <  1:3325631/98‑1 (MQ=255)
                                                           cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CGGCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaaaa                                            >  2:3298710/1‑99 (MQ=2)
                                                           cGTCGCCCCCCCCGCGGGGGCGGGTATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTCCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                           <  2:2250636/101‑1 (MQ=37)
                                                            gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgg                                          >  2:3709900/1‑101 (MQ=255)
                                                            gTCGCCCCCCCCGCGGGGGCGTGGATCAAAACATCTGCATGTAGTGGG.CGG.GCAGTCCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgg                                          <  1:848499/101‑1 (MQ=37)
                                                             tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACTgga                                         >  1:1031621/1‑100 (MQ=255)
                                                             ccGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGt                                         <  1:1816451/100‑1 (MQ=255)
                                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTggga                                        >  2:3414626/1‑99 (MQ=255)
                                                              cGCCCCCCCCGGGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTa                                        <  1:2922167/101‑1 (MQ=255)
                                                                cttcccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGGAACTGGTATg                                      >  2:875290/4‑101 (MQ=255)
                                                                 tCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                     >  2:1603454/1‑101 (MQ=255)
                                                                     cccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCt                                 <  1:3439648/101‑1 (MQ=255)
                                                                     cccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCt                                 <  1:4199048/101‑1 (MQ=255)
                                                                     cccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCt                                 <  2:3153209/101‑1 (MQ=255)
                                                                        ggggggcCGTGAATCGAAACATCTGGTTGTGGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                              <  2:1048267/99‑1 (MQ=37)
                                                                        gcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                              >  1:1403156/1‑101 (MQ=255)
                                                                          gggggCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgcgc                            >  2:3200016/1‑101 (MQ=255)
                                                                           ggggCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCa                           >  2:2084684/1‑101 (MQ=255)
                                                                            gggCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCaa                          >  2:2959342/1‑101 (MQ=255)
                                                                            gggCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCaa                          >  1:3001028/1‑101 (MQ=255)
                                                                             ggCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCaaa                         <  2:433997/101‑1 (MQ=255)
                                                                              gCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAAc                        >  1:2737661/1‑101 (MQ=255)
                                                                                 tGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa                     >  1:3518232/1‑101 (MQ=255)
                                                                                 gggATCGAACCATCTGCACGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa                     <  2:1913152/100‑1 (MQ=255)
                                                                                  gggtCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa                    >  1:2416128/4‑101 (MQ=255)
                                                                                  ggATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa                    <  1:3069006/101‑1 (MQ=255)
                                                                                  ggATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa                    <  1:3194024/101‑1 (MQ=255)
                                                                                  ggATCGAAACAGCTGCATGTAGGCGG.GGG.CCAGTGCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa                    <  2:2476630/101‑1 (MQ=37)
                                                                                   gATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaaa                   >  2:1187649/1‑101 (MQ=255)
                                                                                   gATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaaa                   <  2:3930694/101‑1 (MQ=255)
                                                                                   gATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAATGGTATCGGTCTGGCGCAAACGGaag                   >  2:3092418/1‑100 (MQ=255)
                                                                                       gAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt               <  2:4028524/101‑1 (MQ=255)
                                                                                       gAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt               <  2:2609382/101‑1 (MQ=255)
                                                                                           cATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcg           >  2:2236579/1‑101 (MQ=255)
                                                                                            aTCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgt          >  1:3550556/1‑101 (MQ=255)
                                                                                             tCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgtc         >  2:1643354/1‑101 (MQ=255)
                                                                                              cTGCATGTAGGGGG.CGG.GCAGTTCGA.GCCG.CAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGtcgtcg        <  1:2951518/101‑1 (MQ=255)
                                                                                                gCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCt      >  2:1465308/1‑101 (MQ=255)
                                                                                                 cATGGAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTc     <  1:4114201/101‑1 (MQ=255)
                                                                                                  gtGTGGTCGG.GGG.GGAGTGGGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcc    <  1:2382559/100‑1 (MQ=37)
                                                                                                  aggTAGTCGG.GGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcc    <  1:979659/99‑1 (MQ=255)
                                                                                                  aggTAGTCGG.GGG.GCAGTGCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcc    <  1:1494179/99‑1 (MQ=255)
                                                                                                  aTGTAGTCGG.CGG.GCATTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcc    <  1:3710154/101‑1 (MQ=255)
                                                                                                  aTGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcc    >  2:1290095/1‑101 (MQ=255)
                                                                                                  aTGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcc    <  2:2686805/101‑1 (MQ=255)
                                                                                                    |                                                                                                        
CCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCC  >  NC_014034/1395573‑1395773

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG