Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,674 T→C 6.1% intergenic (+486/‑445) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,6740T→C 6.1% 18.3 ‑0.3 ‑1.3181intergenic (+486/‑445)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/4):  ref base (92/76):  total (99/82)
Fisher's exact test strand distribution p-value = 7.57e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.55e-02

Only 100 of 204 total aligned reads displayed.

CCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCC  >  NC_014034/1395574‑1395774
                                                                                                    |                                                                                                          
cccccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGt                                                                                                            <  2:1799200/101‑1 (MQ=255)
  cccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAg                                                                                                          >  1:2741502/1‑101 (MQ=255)
   ccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGt                                                                                                         <  2:789832/101‑1 (MQ=255)
   ccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGt                                                                                                         <  1:3522220/101‑1 (MQ=255)
   ccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGt                                                                                                         <  1:346838/101‑1 (MQ=255)
    cgcgGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTc                                                                                                        >  2:2382383/1‑101 (MQ=255)
    cgcgGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTc                                                                                                        <  1:3356290/101‑1 (MQ=255)
      cgGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcgg                                                                                                      >  1:960583/1‑101 (MQ=255)
      cgGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcgg                                                                                                      <  2:486335/101‑1 (MQ=255)
       gggggCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcgg.c                                                                                                    <  1:661712/101‑1 (MQ=255)
          ggCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.Cg..g.g                                                                                              >  1:362220/1‑101 (MQ=255)
           gCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGCCGG.CG..G.Gc                                                                                             >  1:2476814/1‑101 (MQ=255)
            cGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCa                                                                                            <  2:2090907/101‑1 (MQ=255)
            cGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCa                                                                                            <  1:627161/101‑1 (MQ=255)
            cGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCa                                                                                            <  2:1150966/101‑1 (MQ=255)
              tGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGt                                                                                          >  2:443055/1‑101 (MQ=255)
              tGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGt                                                                                          <  2:2142036/101‑1 (MQ=255)
              tGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGt                                                                                          <  1:3496503/101‑1 (MQ=255)
              tGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGt                                                                                          <  2:2642983/101‑1 (MQ=255)
                gATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTc                                                                                        <  1:3322825/101‑1 (MQ=255)
                  tCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGa                                                                                      <  1:1083374/101‑1 (MQ=255)
                   cGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.g                                                                                    <  1:546559/101‑1 (MQ=255)
                    gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.Gc                                                                                   >  1:2389132/1‑101 (MQ=255)
                    gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.Gc                                                                                   >  1:1876126/1‑101 (MQ=255)
                     aaaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.Gcc                                                                                  >  1:2537369/1‑101 (MQ=255)
                     aaaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.Gcc                                                                                  >  1:636931/1‑101 (MQ=255)
                      aaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCg                                                                                 >  2:2698740/1‑101 (MQ=255)
                      aaCCACCTTGCGGATTGTAACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCg                                                                                 >  2:524881/1‑101 (MQ=255)
                        cccccTTGCGGATTGTCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.Ca                                                                              <  1:3983094/98‑1 (MQ=255)
                        ccaccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.Ca                                                                              >  2:3412179/1‑101 (MQ=255)
                          accTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAgc                                                                            >  2:4198786/1‑100 (MQ=255)
                           ccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTc                                                                           <  1:1691289/101‑1 (MQ=255)
                              tGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCt                                                                        >  1:893133/1‑101 (MQ=255)
                              ggCGGATTGCCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCt                                                                        <  2:3358922/100‑1 (MQ=255)
                               ggggATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTc                                                                       <  2:660294/99‑1 (MQ=255)
                                cGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTcc                                                                      >  1:1327968/1‑101 (MQ=255)
                                cGGATTCTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCATCTGCATGTAGTCGG.CG..G.GCAGTTCGC.GCCG.CAGTCGCTcc                                                                      >  2:578748/1‑101 (MQ=255)
                                  gATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTcccc                                                                    <  2:174846/101‑1 (MQ=255)
                                   aTTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTccccc                                                                   >  1:591557/1‑101 (MQ=255)
                                   aTTGTCACTTTCGCGGGGCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTccccc                                                                   <  2:3389022/101‑1 (MQ=255)
                                    ttGTCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTcccccc                                                                  <  1:2132095/101‑1 (MQ=255)
                                    ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTcccccc                                                                  <  1:4150070/101‑1 (MQ=255)
                                    ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTcccccc                                                                  <  1:2552502/101‑1 (MQ=255)
                                    ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTcccccc                                                                  <  1:1592225/101‑1 (MQ=255)
                                       tCACTTTCGCGGGGCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCcgcg                                                               <  2:2943881/101‑1 (MQ=255)
                                            ttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGc                                                          <  2:3874758/101‑1 (MQ=255)
                                               gcgGGCCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTg                                                       <  2:2611230/101‑1 (MQ=255)
                                                ggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG..GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTg                                                       >  2:3624233/10‑101 (MQ=2)
                                                ggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG..GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTg                                                       >  2:3429367/10‑101 (MQ=2)
                                                cgGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTgg                                                      >  2:599265/1‑101 (MQ=255)
                                                 gatcccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCCTGACCGCAT.CGA.A.GCTGGTCAC.ATCG.CCGTCGCTCCCCCCGCGGGGGCGTgg                                                      <  2:2197809/91‑1 (MQ=2)
                                                  gggCGAAGCGTCGCCCCCCCCGGGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                    <  1:765578/101‑1 (MQ=255)
                                                   ggCGAAGCGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGGATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                   <  1:2794140/101‑1 (MQ=255)
                                                   gccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG..GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                    >  2:1021792/7‑101 (MQ=2)
                                                   gccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG..GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                    <  1:3901565/95‑1 (MQ=2)
                                                      gAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                >  2:22398/1‑101 (MQ=255)
                                                       gtgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCGGCAG.TGTCA.GGTTTTCCA.GCGG.C.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                <  1:3235548/99‑1 (MQ=2)
                                                       catcGTCGCTCCCCCCGCGGGGACGTGGATCGAATCCTATGAGGGCTGGCGGCCC..G.GTCGTTGAT.CCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                 <  2:3165394/98‑1 (MQ=0)
                                                       aaGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGaac                                               >  2:1892291/1‑100 (MQ=255)
                                                        aGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                              >  1:4028785/1‑101 (MQ=255)
                                                          ggccGCGCCCCCGGGGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                            <  1:598615/98‑1 (MQ=37)
                                                          cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG..GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaaaa                                             >  2:3298710/1‑99 (MQ=2)
                                                           gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgg                                           >  2:3709900/1‑101 (MQ=255)
                                                           gTCGCCCCCCCCGCGGGGGCGTGGATCAAAACATCTGCATGTAGTGGG.CG..G.GCAGTCCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgg                                           <  1:848499/101‑1 (MQ=37)
                                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACTgga                                          >  1:1031621/1‑100 (MQ=255)
                                                             cGCCCCCCCCGGGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTa                                         <  1:2922167/101‑1 (MQ=255)
                                                               cttcccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGGAACTGGTATg                                       >  2:875290/4‑101 (MQ=255)
                                                                tCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                      >  2:1603454/1‑101 (MQ=255)
                                                                tCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                      >  2:1172760/1‑101 (MQ=255)
                                                                cccccccGGGGGGGGGGGGATCAAAACACCTGCTTGTAGTGGG.CG..G.GCGGTCGGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                      <  1:3873470/100‑1 (MQ=21)
                                                                    cccGCGGGGGGGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCt                                  <  1:2698946/101‑1 (MQ=255)
                                                                    cccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCt                                  <  2:3153209/101‑1 (MQ=255)
                                                                      cgcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTgg                                >  1:2612570/1‑101 (MQ=255)
                                                                      cgcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTgg                                <  2:2499199/101‑1 (MQ=255)
                                                                       gcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                               >  1:1403156/1‑101 (MQ=255)
                                                                         gggggCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCT.CAGTCGATTCCCCCGCGGGGGCGTGGATCGAAACTGTTATGGATGTgactc                             >  2:2072258/1‑97 (MQ=37)
                                                                         gggggCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgcgc                             >  2:3200016/1‑101 (MQ=255)
                                                                           gggCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCaa                           >  1:3001028/1‑101 (MQ=255)
                                                                            ggCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCaaa                          <  2:433997/101‑1 (MQ=255)
                                                                              cGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACg                        >  1:1783396/1‑101 (MQ=255)
                                                                               gTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACgg                       <  1:3185690/101‑1 (MQ=255)
                                                                                 ggATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa                     <  1:3069006/101‑1 (MQ=255)
                                                                                 ggATCGAAACATCTGCAGGTGGTCGG.CG..G.GCAGTGCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa                     <  2:1818926/101‑1 (MQ=255)
                                                                                  gATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaaa                    <  2:3930694/101‑1 (MQ=255)
                                                                                  gATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAATGGTATCGGTCTGGCGCAAACGGaag                    >  2:3092418/1‑100 (MQ=255)
                                                                                      gAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt                <  2:4028524/101‑1 (MQ=255)
                                                                                      gAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt                >  1:2364363/1‑101 (MQ=255)
                                                                                        aaCATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgt              <  1:2043358/101‑1 (MQ=255)
                                                                                        aaCATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgt              <  2:1270110/101‑1 (MQ=255)
                                                                                            tCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgtc          >  2:3507926/1‑101 (MQ=255)
                                                                                            tCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgtc          >  2:1643354/1‑101 (MQ=255)
                                                                                             cTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGtcgtcg         <  1:4113891/101‑1 (MQ=255)
                                                                                             cTGCATGTAGGGGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGtcgtcg         <  1:2951518/101‑1 (MQ=255)
                                                                                               gCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCt       >  2:1465308/1‑101 (MQ=255)
                                                                                               gCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCt       >  2:1925088/1‑101 (MQ=255)
                                                                                               gCATGTAGCCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCt       <  2:1876006/101‑1 (MQ=255)
                                                                                                cATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTc      <  1:443112/101‑1 (MQ=255)
                                                                                                 aggTAGTCGG.GG..G.GCAGTGCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcc     <  1:1494179/99‑1 (MQ=255)
                                                                                                   gTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcccc   >  1:179460/1‑101 (MQ=255)
                                                                                                    tAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTccccc  >  2:129530/1‑101 (MQ=255)
                                                                                                    |                                                                                                          
CCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCC  >  NC_014034/1395574‑1395774

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG