Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,685 C→A 5.9% intergenic (+497/‑434) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,6850C→A 5.9% 16.2 ‑0.3 0.0179intergenic (+497/‑434)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/4):  ref base (90/77):  total (97/82)
Fisher's exact test strand distribution p-value = 7.56e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 202 total aligned reads displayed.

GCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGT  >  NC_014034/1395585‑1395785
                                                                                                        |                                                                                                      
gCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGCCGG.CG..G.Gc                                                                                                        >  1:2476814/1‑101 (MQ=255)
 cGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCa                                                                                                       <  2:2090907/101‑1 (MQ=255)
 cGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCa                                                                                                       <  1:627161/101‑1 (MQ=255)
   tGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGt                                                                                                     <  2:2642983/101‑1 (MQ=255)
    ggATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGtt                                                                                                    >  2:4113940/1‑101 (MQ=255)
       tCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGa                                                                                                 <  1:1083374/101‑1 (MQ=255)
       tCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGa                                                                                                 >  2:1737025/1‑101 (MQ=255)
       tCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGa                                                                                                 <  1:4022781/101‑1 (MQ=255)
        cGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.g                                                                                               <  1:546559/101‑1 (MQ=255)
         gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.Gc                                                                                              >  1:1876126/1‑101 (MQ=255)
         gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.Gc                                                                                              >  2:1430809/1‑101 (MQ=255)
         gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.Gc                                                                                              >  1:2611421/1‑101 (MQ=255)
          aaaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.Gcc                                                                                             >  1:3930954/1‑101 (MQ=255)
          aaaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.Gcc                                                                                             >  1:636931/1‑101 (MQ=255)
           aaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCg                                                                                            >  2:2698740/1‑101 (MQ=255)
           aaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCg                                                                                            >  1:2944103/1‑101 (MQ=255)
             ccaccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.Ca                                                                                         >  2:3412179/1‑101 (MQ=255)
               accTTGCGGATTGTCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGt                                                                                       <  2:440112/101‑1 (MQ=255)
                ccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTc                                                                                      <  2:1794268/101‑1 (MQ=255)
                ccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTc                                                                                      <  1:1691289/101‑1 (MQ=255)
                   tGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCt                                                                                   >  1:893133/1‑101 (MQ=255)
                   ggCGGATTGCCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCt                                                                                   <  2:3358922/100‑1 (MQ=255)
                    ggggATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTc                                                                                  <  2:660294/99‑1 (MQ=255)
                     cGGATTCTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCATCTGCATGTAGTCGG.CG..G.GCAGTTCGC.GCCG.CAGTCGCTcc                                                                                 >  2:578748/1‑101 (MQ=255)
                       gATTGTCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTcccc                                                                               <  1:664524/101‑1 (MQ=255)
                       gATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTcccc                                                                               <  1:1557197/101‑1 (MQ=255)
                        aTTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTccccc                                                                              >  1:591557/1‑101 (MQ=255)
                        aTTGTCACTTTCGCGGGGCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTccccc                                                                              <  2:3389022/101‑1 (MQ=255)
                         ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTcccccc                                                                             >  2:1159018/1‑101 (MQ=255)
                         ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTcccccc                                                                             <  2:2154351/101‑1 (MQ=255)
                         ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTcccccc                                                                             <  1:4150070/101‑1 (MQ=255)
                            tCACTTTCGCGGGGCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCcgcg                                                                          <  2:2943881/101‑1 (MQ=255)
                                tttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCggggg                                                                      >  1:2091141/1‑101 (MQ=255)
                                tttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCggggg                                                                      >  2:429380/1‑101 (MQ=255)
                                 ttCGCGGGGCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGc                                                                     <  2:48514/101‑1 (MQ=255)
                                    gcgGGCCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTg                                                                  <  2:2611230/101‑1 (MQ=255)
                                     ggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG..GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTg                                                                  >  2:3429367/10‑101 (MQ=2)
                                     ggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG..GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTg                                                                  >  1:2920285/10‑101 (MQ=2)
                                     gggggCGAGGCGGCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTgg                                                                 <  2:1832047/100‑1 (MQ=255)
                                     gggggCGAAGCGTCCCCCCCCCCGCGGGGGCGGGGATCGAAACATCTGCATGTGGTCGG.CG..G.GGAGTTGGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTgg                                                                 <  1:3893157/100‑1 (MQ=38)
                                     cgGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTgg                                                                 >  2:599265/1‑101 (MQ=255)
                                      ggggCGAAGCGTCGCTCCCCCGGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGa                                                                >  1:3114965/1‑101 (MQ=255)
                                      ggggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGa                                                                <  1:348264/101‑1 (MQ=255)
                                      ggggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGa                                                                >  2:3654634/1‑101 (MQ=255)
                                      ggggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGa                                                                >  1:2549836/1‑101 (MQ=255)
                                        ggCGAAGCGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGGATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                              <  1:2794140/101‑1 (MQ=255)
                                           gAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                           >  1:1270206/1‑101 (MQ=255)
                                            gtgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCGGCAG.TGTCA.GGTTTTCCA.GCGG.C.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                           <  1:3235548/99‑1 (MQ=2)
                                            ctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG..GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                           <  1:4104645/99‑1 (MQ=2)
                                            catcGTCGCTCCCCCCGCGGGGACGTGGATCGAATCCTATGAGGGCTGGCGGCCC..G.GTCGTTGAT.CCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                            <  2:3165394/98‑1 (MQ=0)
                                            aaGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGaac                                                          >  2:1892291/1‑100 (MQ=255)
                                             tgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG..GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                          >  1:2362919/2‑101 (MQ=2)
                                              gCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                        >  1:2687011/1‑101 (MQ=255)
                                               ggccGCGCCCCCGGGGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                                       <  1:598615/98‑1 (MQ=37)
                                               cggcGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                                       <  1:3325631/98‑1 (MQ=255)
                                               cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                                       >  1:14741/1‑101 (MQ=255)
                                               cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG..GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaaaa                                                        >  2:3298710/1‑99 (MQ=2)
                                               cGTCGCCCCCCCCGCGGGGGCGGGTATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTCCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                                       <  2:2250636/101‑1 (MQ=37)
                                                gTCGCCCCCCCCGCGGGGGCGTGGATCAAAACATCTGCATGTAGTGGG.CG..G.GCAGTCCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgg                                                      <  1:848499/101‑1 (MQ=37)
                                                 tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACTgga                                                     >  1:1031621/1‑100 (MQ=255)
                                                 tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGt                                                     <  1:2778662/101‑1 (MQ=255)
                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTa                                                    >  1:3056867/1‑101 (MQ=255)
                                                    cttcccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGGAACTGGTATg                                                  >  2:875290/4‑101 (MQ=255)
                                                     tCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                                 >  1:2966343/1‑101 (MQ=255)
                                                         cccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCt                                             <  1:3439648/101‑1 (MQ=255)
                                                         cccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCt                                             <  2:3153209/101‑1 (MQ=255)
                                                           cgcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTgg                                           <  2:2499199/101‑1 (MQ=255)
                                                            ggggggcCGTGAATCGAAACATCTGGTTGTGGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                                          <  2:1048267/99‑1 (MQ=37)
                                                            gcgGGGGCGTGGACCGAAACATCGGCGGGGAGTCGG.CG..G.GCAGTTGGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                                          <  2:4017738/101‑1 (MQ=37)
                                                              gggggCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCT.CAGTCGATTCCCCCGCGGGGGCGTGGATCGAAACTGTTATGGATGTgactc                                        >  2:2072258/1‑97 (MQ=37)
                                                              gggggCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgcgc                                        >  2:3200016/1‑101 (MQ=255)
                                                                  gCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAAc                                    >  1:2737661/1‑101 (MQ=255)
                                                                   cGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACg                                   >  1:1783396/1‑101 (MQ=255)
                                                                   cGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACg                                   >  2:3742661/1‑101 (MQ=255)
                                                                    gTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACgg                                  <  1:3185690/101‑1 (MQ=255)
                                                                     tGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa                                 >  1:3518232/1‑101 (MQ=255)
                                                                     tGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa                                 >  2:1629877/1‑101 (MQ=255)
                                                                       gATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaaa                               >  2:1187649/1‑101 (MQ=255)
                                                                       gATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAATGGTATCGGTCTGGCGCAAACGGaag                               >  2:3092418/1‑100 (MQ=255)
                                                                          cGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTc                            >  1:964882/1‑101 (MQ=255)
                                                                          cGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCCTGGATCGAAACTGGTATGGATCTGGCGTGAACGGAAAGTc                            >  1:1398615/1‑101 (MQ=255)
                                                                           gAAACACCGGCAGGTAGGCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt                           <  2:3901031/101‑1 (MQ=37)
                                                                               cATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcg                       >  2:2236579/1‑101 (MQ=255)
                                                                                 tCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgtc                     >  2:3507926/1‑101 (MQ=255)
                                                                                    gCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCt                  >  2:1925088/1‑101 (MQ=255)
                                                                                     cATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTc                 <  1:443112/101‑1 (MQ=255)
                                                                                     cATGGAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTc                 <  1:4114201/101‑1 (MQ=255)
                                                                                      gtGTGGTCGG.GG..G.GGAGTGGGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcc                <  1:2382559/100‑1 (MQ=37)
                                                                                      aTGTAGTCGG.CG..G.GCATTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcc                <  1:3710154/101‑1 (MQ=255)
                                                                                      aTGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcc                >  2:1561747/1‑101 (MQ=255)
                                                                                      aTGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcc                >  2:1290095/1‑101 (MQ=255)
                                                                                           gTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCcg           >  1:1802356/1‑101 (MQ=255)
                                                                                           gTCGG.CG..G.GCAGTCCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCcg           <  2:4030918/101‑1 (MQ=255)
                                                                                            gcgg.cg..g.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCcgc          <  2:3620668/100‑1 (MQ=255)
                                                                                             cgg.cg..g.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCcgcg         >  1:2390898/1‑101 (MQ=255)
                                                                                             cgg.cg..g.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCcgcg         >  2:736160/1‑101 (MQ=255)
                                                                                              gg.cg..g.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCgg        >  1:3280824/1‑101 (MQ=255)
                                                                                              gg.cg..g.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCgg        >  2:303445/1‑101 (MQ=255)
                                                                                                     g.gCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGc    <  2:1327868/101‑1 (MQ=255)
                                                                                                        cAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGTTATTGATCTGGCGCAAACGGAAAGTCTGCCGTCGCTCCCCCCGCGGGGGCGt  >  2:536582/1‑101 (MQ=255)
                                                                                                        |                                                                                                      
GCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGT  >  NC_014034/1395585‑1395785

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG