Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,687 G→C 6.2% intergenic (+499/‑432) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,6870G→C 6.2% 17.3 ‑0.3 ‑0.2179intergenic (+499/‑432)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/4):  ref base (94/73):  total (101/78)
Fisher's exact test strand distribution p-value = 7.59e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.83e-01

Only 100 of 201 total aligned reads displayed.

TGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTG  >  NC_014034/1395588‑1395786
                                                                                                     |                                                                                                     
tGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGt                                                                                                      <  1:3496503/101‑1 (MQ=255)
tGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGt                                                                                                      <  2:2642983/101‑1 (MQ=255)
 ggATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGtt                                                                                                     >  2:4113940/1‑101 (MQ=255)
 ggATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGtt                                                                                                     >  1:890488/1‑101 (MQ=255)
  gATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTc                                                                                                    <  1:3322825/101‑1 (MQ=255)
    tCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGa                                                                                                  <  1:548626/101‑1 (MQ=255)
      gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.Gc                                                                                               >  1:2611421/1‑101 (MQ=255)
      gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.Gc                                                                                               <  2:1936796/101‑1 (MQ=255)
      gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.Gc                                                                                               >  1:2389132/1‑101 (MQ=255)
       aaaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.Gcc                                                                                              >  1:636931/1‑101 (MQ=255)
        aaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCg                                                                                             >  2:2698740/1‑101 (MQ=255)
        aaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCg                                                                                             <  1:1037329/101‑1 (MQ=255)
        aaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCg                                                                                             >  1:2944103/1‑101 (MQ=255)
          cccccTTGCGGATTGTCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.Ca                                                                                          <  1:3983094/98‑1 (MQ=255)
          ccaccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.Ca                                                                                          >  2:3412179/1‑101 (MQ=255)
            accTTGCGGATTGTCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGt                                                                                        <  2:440112/101‑1 (MQ=255)
             ccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTc                                                                                       >  1:479137/1‑101 (MQ=255)
                tGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCt                                                                                    <  1:1699823/101‑1 (MQ=255)
                ggCGGATTGCCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCt                                                                                    <  2:3358922/100‑1 (MQ=255)
                 ggggATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTc                                                                                   <  2:660294/99‑1 (MQ=255)
                  cGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTcc                                                                                  >  1:1327968/1‑101 (MQ=255)
                  cGGATTCTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCATCTGCATGTAGTCGG.CGG.GCAGTTCGC.GCCG.CAGTCGCTcc                                                                                  >  2:578748/1‑101 (MQ=255)
                    gATTGTCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTcccc                                                                                <  1:664524/101‑1 (MQ=255)
                    gATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTcccc                                                                                <  2:174846/101‑1 (MQ=255)
                     aTTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTccccc                                                                               >  1:591557/1‑101 (MQ=255)
                     aTTGTCACTTTCGCGGGGCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTccccc                                                                               <  2:3389022/101‑1 (MQ=255)
                      ttGTCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTcccccc                                                                              <  1:2132095/101‑1 (MQ=255)
                      ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTcccccc                                                                              <  2:2154351/101‑1 (MQ=255)
                      ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTcccccc                                                                              <  1:1592225/101‑1 (MQ=255)
                       tGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCcg                                                                             >  2:2614954/1‑101 (MQ=255)
                         tCACTTTCGCGGGGCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCcgcg                                                                           <  2:2943881/101‑1 (MQ=255)
                             tttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCggggg                                                                       >  2:429380/1‑101 (MQ=255)
                              ttCGCGGGGCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGc                                                                      <  2:48514/101‑1 (MQ=255)
                                  ggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CGGCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTg                                                                   >  2:3429367/10‑101 (MQ=2)
                                  ggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CGGCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTg                                                                   >  1:3257903/10‑101 (MQ=2)
                                  ggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CGGCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTg                                                                   >  1:2920285/10‑101 (MQ=2)
                                  cgGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTgg                                                                  >  2:599265/1‑101 (MQ=255)
                                   ggggCGAAGCGTCGCTCCCCCGGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGa                                                                 >  1:3114965/1‑101 (MQ=255)
                                   ggggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGa                                                                 >  2:3654634/1‑101 (MQ=255)
                                   ggggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGa                                                                 >  1:2549836/1‑101 (MQ=255)
                                     ggCGAAGCGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGGATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                               <  1:2794140/101‑1 (MQ=255)
                                     gccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CGGCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                <  1:3901565/95‑1 (MQ=2)
                                         catcGTCGCTCCCCCCGCGGGGACGTGGATCGAATCCTATGAGGGCTGGCGGCCCG.GTCGTTGAT.CCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                             <  2:3165394/98‑1 (MQ=0)
                                          aGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                          >  1:4028785/1‑101 (MQ=255)
                                           gCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                         >  1:2687011/1‑101 (MQ=255)
                                            ggccGCGCCCCCGGGGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                                        <  1:598615/98‑1 (MQ=37)
                                            cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CGGCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaaaa                                                         >  2:3298710/1‑99 (MQ=2)
                                            cGTCGCCCCCCCCGCGGGGGCGGGTATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTCCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                                        <  2:2250636/101‑1 (MQ=37)
                                              tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGt                                                      <  1:2778662/101‑1 (MQ=255)
                                              ccGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGt                                                      <  1:1816451/100‑1 (MQ=255)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTa                                                     >  1:3056867/1‑101 (MQ=255)
                                                 cttcccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGGAACTGGTATg                                                   >  2:875290/4‑101 (MQ=255)
                                                  tCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                                  >  2:1172760/1‑101 (MQ=255)
                                                  tCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                                  >  1:2966343/1‑101 (MQ=255)
                                                  tCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                                  >  2:1603454/1‑101 (MQ=255)
                                                  cccccccGGGGGGGGGGGGATCAAAACACCTGCTTGTAGTGGG.CGG.GCGGTCGGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                                  <  1:3873470/100‑1 (MQ=21)
                                                      cccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCt                                              <  2:3153209/101‑1 (MQ=255)
                                                      cccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCt                                              <  1:4199048/101‑1 (MQ=255)
                                                        cgcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTgg                                            <  2:2499199/101‑1 (MQ=255)
                                                         ggggggcCGTGAATCGAAACATCTGGTTGTGGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                                           <  2:1048267/99‑1 (MQ=37)
                                                         gcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                                           >  1:1403156/1‑101 (MQ=255)
                                                         gcgGGGGCGTGGACCGAAACATCGGCGGGGAGTCGG.CGG.GCAGTTGGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                                           <  2:4017738/101‑1 (MQ=37)
                                                           gggggCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgcgc                                         >  2:3200016/1‑101 (MQ=255)
                                                            ggggCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCa                                        >  2:2084684/1‑101 (MQ=255)
                                                               gCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAAc                                     >  1:2737661/1‑101 (MQ=255)
                                                                cGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACg                                    >  2:3742661/1‑101 (MQ=255)
                                                                  gggATCGAACCATCTGCACGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa                                  <  2:1913152/100‑1 (MQ=255)
                                                                   gggtCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa                                 >  1:2416128/4‑101 (MQ=255)
                                                                   ggATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa                                 <  1:3194024/101‑1 (MQ=255)
                                                                   ggATCGAAACATCTGCAGGTGGTCGG.CGG.GCAGTGCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa                                 <  2:1818926/101‑1 (MQ=255)
                                                                    gATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAATGGTATCGGTCTGGCGCAAACGGaag                                >  2:3092418/1‑100 (MQ=255)
                                                                        gAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt                            <  2:2609382/101‑1 (MQ=255)
                                                                        gAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt                            >  1:2364363/1‑101 (MQ=255)
                                                                        gAAACACCGGCAGGTAGGCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt                            <  2:3901031/101‑1 (MQ=37)
                                                                          aaCATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgt                          <  2:1270110/101‑1 (MQ=255)
                                                                          aaCATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgt                          <  1:2043358/101‑1 (MQ=255)
                                                                            cATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcg                        >  2:2236579/1‑101 (MQ=255)
                                                                             aTCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgt                       >  1:3550556/1‑101 (MQ=255)
                                                                              tCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgtc                      >  2:1643354/1‑101 (MQ=255)
                                                                              tCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgtc                      >  2:3507926/1‑101 (MQ=255)
                                                                              tCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTggcgtc                      >  1:919872/1‑101 (MQ=255)
                                                                               cTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGtcgtcg                     <  1:4113891/101‑1 (MQ=255)
                                                                                 gCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCt                   >  2:1465308/1‑101 (MQ=255)
                                                                                 gCATGTAGCCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCt                   <  2:1876006/101‑1 (MQ=255)
                                                                                   aggTAGTCGG.GGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcc                 <  1:979659/99‑1 (MQ=255)
                                                                                   aggTAGTCGG.GGG.GCAGTGCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcc                 <  1:1494179/99‑1 (MQ=255)
                                                                                     gTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcccc               >  1:179460/1‑101 (MQ=255)
                                                                                      tAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTccccc              >  2:129530/1‑101 (MQ=255)
                                                                                        gTCGG.CGG.GCAGTCCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCcg            <  2:4030918/101‑1 (MQ=255)
                                                                                         gcgg.cgg.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCcgc           <  2:3620668/100‑1 (MQ=255)
                                                                                          cgg.cgg.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCcgcg          >  2:736160/1‑101 (MQ=255)
                                                                                           gg.cgg.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCgg         >  2:303445/1‑101 (MQ=255)
                                                                                           gg.cgg.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCgg         >  1:336526/1‑101 (MQ=255)
                                                                                            g.cgg.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCggg        >  1:3469124/1‑101 (MQ=255)
                                                                                              ggg.gCGGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCgggg       <  1:1172854/100‑1 (MQ=255)
                                                                                               gg.gCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCggggg      >  2:1402118/1‑101 (MQ=255)
                                                                                               gg.gCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCggggg      <  1:4122783/101‑1 (MQ=255)
                                                                                                   cAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGTTATTGATCTGGCGCAAACGGAAAGTCTGCCGTCGCTCCCCCCGCGGGGGCGt   >  2:536582/1‑101 (MQ=255)
                                                                                                    aGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTg  >  1:816544/1‑101 (MQ=255)
                                                                                                    aGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTg  <  1:1015688/101‑1 (MQ=255)
                                                                                                     |                                                                                                     
TGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CGG.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTG  >  NC_014034/1395588‑1395786

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG