Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,688 T→A 6.4% intergenic (+500/‑431) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,6880T→A 6.4% 18.8 ‑0.3 ‑0.0173intergenic (+500/‑431)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/4):  ref base (91/70):  total (98/75)
Fisher's exact test strand distribution p-value = 7.59e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.50e-01

Only 100 of 203 total aligned reads displayed.

TGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGA  >  NC_014034/1395588‑1395788
                                                                                                        |                                                                                                      
tGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGt                                                                                                        <  2:2642983/101‑1 (MQ=255)
 ggATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGtt                                                                                                       >  1:890488/1‑101 (MQ=255)
 ggATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGtt                                                                                                       >  2:4113940/1‑101 (MQ=255)
    tCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGa                                                                                                    <  1:548626/101‑1 (MQ=255)
      gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.Gc                                                                                                 <  2:1936796/101‑1 (MQ=255)
      gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.Gc                                                                                                 >  1:1876126/1‑101 (MQ=255)
       aaaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.Gcc                                                                                                >  1:2537369/1‑101 (MQ=255)
       aaaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.Gcc                                                                                                >  1:636931/1‑101 (MQ=255)
        aaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCg                                                                                               >  2:2698740/1‑101 (MQ=255)
        aaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCg                                                                                               <  1:1037329/101‑1 (MQ=255)
          cccccTTGCGGATTGTCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.Ca                                                                                            <  1:3983094/98‑1 (MQ=255)
          ccaccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.Ca                                                                                            >  2:3412179/1‑101 (MQ=255)
            accTTGCGGATTGTCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGt                                                                                          <  2:440112/101‑1 (MQ=255)
             ccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTc                                                                                         <  1:1691289/101‑1 (MQ=255)
             ccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTc                                                                                         >  1:479137/1‑101 (MQ=255)
                ggCGGATTGCCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCt                                                                                      <  2:3358922/100‑1 (MQ=255)
                 ggggATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTc                                                                                     <  2:660294/99‑1 (MQ=255)
                  cGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTcc                                                                                    >  1:1327968/1‑101 (MQ=255)
                  cGGATTCTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCATCTGCATGTAGTCGG.CG..G.GCAGTTCGC.GCCG.CAGTCGCTcc                                                                                    >  2:578748/1‑101 (MQ=255)
                    gATTGTCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTcccc                                                                                  <  1:664524/101‑1 (MQ=255)
                    gATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTcccc                                                                                  <  2:174846/101‑1 (MQ=255)
                    gATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTcccc                                                                                  <  1:1557197/101‑1 (MQ=255)
                     aTTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTccccc                                                                                 >  1:591557/1‑101 (MQ=255)
                     aTTGTCACTTTCGCGGGGCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTccccc                                                                                 <  2:3389022/101‑1 (MQ=255)
                      ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTcccccc                                                                                <  2:2154351/101‑1 (MQ=255)
                      ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTcccccc                                                                                <  1:2552502/101‑1 (MQ=255)
                       tGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCcg                                                                               >  2:2614954/1‑101 (MQ=255)
                         tCACTTTCGCGGGGCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCcgcg                                                                             <  2:2943881/101‑1 (MQ=255)
                             tttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCggggg                                                                         >  2:429380/1‑101 (MQ=255)
                             tttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCggggg                                                                         >  1:2091141/1‑101 (MQ=255)
                              ttCGCGGGGCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGc                                                                        <  2:48514/101‑1 (MQ=255)
                                  ggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG..GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTg                                                                     >  2:3429367/10‑101 (MQ=2)
                                  cgGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTgg                                                                    >  2:599265/1‑101 (MQ=255)
                                   ggggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGa                                                                   >  2:3654634/1‑101 (MQ=255)
                                   ggggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGa                                                                   <  1:348264/101‑1 (MQ=255)
                                     gccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG..GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                  <  1:3901565/95‑1 (MQ=2)
                                         gtgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCGGCAG.TGTCA.GGTTTTCCA.GCGG.C.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                              <  1:3235548/99‑1 (MQ=2)
                                         catcGTCGCTCCCCCCGCGGGGACGTGGATCGAATCCTATGAGGGCTGGCGGCCC..G.GTCGTTGAT.CCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                               <  2:3165394/98‑1 (MQ=0)
                                          tgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG..GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                             >  1:2362919/2‑101 (MQ=2)
                                          aGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                            >  1:4028785/1‑101 (MQ=255)
                                            ggccGCGCCCCCGGGGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                                          <  1:598615/98‑1 (MQ=37)
                                            cggcGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                                          <  1:3325631/98‑1 (MQ=255)
                                            cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG..GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaaaa                                                           >  2:3298710/1‑99 (MQ=2)
                                            cGTCGCCCCCCCCGCGGGGGCGGGTATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTCCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                                          <  2:2250636/101‑1 (MQ=37)
                                               cGCCCCCCCCGGGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTa                                                       <  1:2922167/101‑1 (MQ=255)
                                                 cttcccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGGAACTGGTATg                                                     >  2:875290/4‑101 (MQ=255)
                                                  tCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                                    >  2:1172760/1‑101 (MQ=255)
                                                  tCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                                    >  2:1603454/1‑101 (MQ=255)
                                                      cccGCGGGGGGGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCt                                                <  1:2698946/101‑1 (MQ=255)
                                                      cccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCt                                                <  1:4199048/101‑1 (MQ=255)
                                                      cccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCt                                                <  1:3439648/101‑1 (MQ=255)
                                                      cccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCt                                                <  2:3153209/101‑1 (MQ=255)
                                                        cgcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTgg                                              <  2:2499199/101‑1 (MQ=255)
                                                        cgcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTgg                                              >  1:2612570/1‑101 (MQ=255)
                                                         ggggggcCGTGAATCGAAACATCTGGTTGTGGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                                             <  2:1048267/99‑1 (MQ=37)
                                                         gcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                                             >  1:1403156/1‑101 (MQ=255)
                                                         gcgGGGGCGTGGACCGAAACATCGGCGGGGAGTCGG.CG..G.GCAGTTGGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                                             <  2:4017738/101‑1 (MQ=37)
                                                           gggggCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgcgc                                           >  2:3200016/1‑101 (MQ=255)
                                                            ggggCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCa                                          >  2:2084684/1‑101 (MQ=255)
                                                             gggCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCaa                                         >  1:3001028/1‑101 (MQ=255)
                                                                cGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACg                                      >  1:1783396/1‑101 (MQ=255)
                                                                cGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACg                                      >  2:3742661/1‑101 (MQ=255)
                                                                 gTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACgg                                     <  1:3185690/101‑1 (MQ=255)
                                                                  tGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa                                    >  1:3518232/1‑101 (MQ=255)
                                                                  gggATCGAACCATCTGCACGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa                                    <  2:1913152/100‑1 (MQ=255)
                                                                   ggATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa                                   <  1:3069006/101‑1 (MQ=255)
                                                                   ggATCGAAACATCTGCAGGTGGTCGG.CG..G.GCAGTGCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa                                   <  2:1818926/101‑1 (MQ=255)
                                                                    gATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAATGGTATCGGTCTGGCGCAAACGGaag                                  >  2:3092418/1‑100 (MQ=255)
                                                                        gAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt                              <  2:2609382/101‑1 (MQ=255)
                                                                        gAAACACCGGCAGGTAGGCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt                              <  2:3901031/101‑1 (MQ=37)
                                                                          aaCATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgt                            <  2:1270110/101‑1 (MQ=255)
                                                                            cATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcg                          >  2:2236579/1‑101 (MQ=255)
                                                                              tCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgtc                        >  2:3507926/1‑101 (MQ=255)
                                                                              tCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgtc                        >  2:1643354/1‑101 (MQ=255)
                                                                              tCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTggcgtc                        >  1:919872/1‑101 (MQ=255)
                                                                               cTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGtcgtcg                       <  1:4113891/101‑1 (MQ=255)
                                                                               cTGCATGTAGGGGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGtcgtcg                       <  1:2951518/101‑1 (MQ=255)
                                                                                 gCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCt                     >  2:1465308/1‑101 (MQ=255)
                                                                                 gCATGTAGCCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCt                     <  2:1876006/101‑1 (MQ=255)
                                                                                   gtGTGGTCGG.GG..G.GGAGTGGGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcc                   <  1:2382559/100‑1 (MQ=37)
                                                                                   aggTAGTCGG.GG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcc                   <  1:979659/99‑1 (MQ=255)
                                                                                   aggTAGTCGG.GG..G.GCAGTGCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcc                   <  1:1494179/99‑1 (MQ=255)
                                                                                   aTGTAGTCGG.CG..G.GCATTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcc                   <  1:3710154/101‑1 (MQ=255)
                                                                                      tAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTccccc                >  2:129530/1‑101 (MQ=255)
                                                                                        gTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCcg              >  1:1802356/1‑101 (MQ=255)
                                                                                        gTCGG.CG..G.GCAGTCCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCcg              <  2:4030918/101‑1 (MQ=255)
                                                                                         gcgg.cg..g.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCcgc             <  2:3620668/100‑1 (MQ=255)
                                                                                          cgg.cg..g.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCcgcg            >  1:275299/1‑101 (MQ=255)
                                                                                          cgg.cg..g.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCcgcg            >  1:2390898/1‑101 (MQ=255)
                                                                                          cgg.cg..g.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCcgcg            >  2:736160/1‑101 (MQ=255)
                                                                                           gg.cg..g.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCgg           >  1:2788767/1‑101 (MQ=255)
                                                                                           gg.cg..g.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCgg           >  1:3280824/1‑101 (MQ=255)
                                                                                           gg.cg..g.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCgg           >  2:303445/1‑101 (MQ=255)
                                                                                              gg..g.gCGGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCgggg         <  1:1172854/100‑1 (MQ=255)
                                                                                               g..g.gCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCggggg        <  1:4122783/101‑1 (MQ=255)
                                                                                               g..g.gCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCggggg        >  2:1402118/1‑101 (MQ=255)
                                                                                                     cAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGTTATTGATCTGGCGCAAACGGAAAGTCTGCCGTCGCTCCCCCCGCGGGGGCGt     >  2:536582/1‑101 (MQ=255)
                                                                                                     cAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGt     >  1:2812043/1‑101 (MQ=255)
                                                                                                      aGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTg    >  1:816544/1‑101 (MQ=255)
                                                                                                      aGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTg    <  1:1015688/101‑1 (MQ=255)
                                                                                                        |                                                                                                      
TGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGA  >  NC_014034/1395588‑1395788

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG