Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,693 G→A 6.5% intergenic (+505/‑426) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,6930G→A 6.5% 20.5 0.0 ‑0.0185intergenic (+505/‑426)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/5):  ref base (93/80):  total (100/85)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.99e-01

Only 100 of 201 total aligned reads displayed.

CGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1395593‑1395793
                                                                                                         |                                                                                                     
 gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGC.Gc                                                                                                      >  2:3439396/1‑101 (MQ=255)
 gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.Gc                                                                                                      <  2:1936796/101‑1 (MQ=255)
 gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.Gc                                                                                                      >  1:1876126/1‑101 (MQ=255)
  aaaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.Gcc                                                                                                     >  1:2537369/1‑101 (MQ=255)
  aaaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.Gcc                                                                                                     >  1:636931/1‑101 (MQ=255)
   aaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCg                                                                                                    <  1:1037329/101‑1 (MQ=255)
   aaCCACCTTGCGGATTGTAACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCg                                                                                                    >  2:524881/1‑101 (MQ=255)
     cccccTTGCGGATTGTCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.Ca                                                                                                 <  1:3983094/98‑1 (MQ=255)
       accTTGCGGATTGTCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGt                                                                                               <  2:440112/101‑1 (MQ=255)
        ccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTc                                                                                              <  2:1794268/101‑1 (MQ=255)
         cTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCg                                                                                             <  2:877191/101‑1 (MQ=255)
           tGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCt                                                                                           <  1:1699823/101‑1 (MQ=255)
           tGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCt                                                                                           >  1:893133/1‑101 (MQ=255)
               gATTGTCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTcccc                                                                                       <  1:664524/101‑1 (MQ=255)
                 ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTcccccc                                                                                     <  2:3398002/101‑1 (MQ=255)
                 ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTcccccc                                                                                     <  1:1592225/101‑1 (MQ=255)
                 ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTcccccc                                                                                     <  1:2552502/101‑1 (MQ=255)
                 ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTcccccc                                                                                     <  1:4150070/101‑1 (MQ=255)
                  tGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCcg                                                                                    >  2:2614954/1‑101 (MQ=255)
                    tCACTTTCGCGGGGCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCcgcg                                                                                  <  2:2943881/101‑1 (MQ=255)
                        tttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCggggg                                                                              >  2:429380/1‑101 (MQ=255)
                        tttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCggggg                                                                              >  1:2091141/1‑101 (MQ=255)
                         ttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGc                                                                             <  2:3874758/101‑1 (MQ=255)
                             ggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG..GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTg                                                                          >  2:3624233/10‑101 (MQ=2)
                             gggggCGAGGCGGCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTgg                                                                         <  2:1832047/100‑1 (MQ=255)
                              gatcccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCCTGACCGCAT.CGA.A.GCTGGTCAC.ATCG.CCGTCGCTCCCCCCGCGGGGGCGTgg                                                                         <  2:2197809/91‑1 (MQ=2)
                                gccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG..GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                       <  1:3901565/95‑1 (MQ=2)
                                gccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG..GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                       <  2:2750288/95‑1 (MQ=2)
                                 gCGAGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                     <  2:3346603/101‑1 (MQ=255)
                                 ccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG..GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                      <  2:3387133/96‑1 (MQ=2)
                                   gAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                   >  1:1270206/1‑101 (MQ=255)
                                   gAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                   >  2:22398/1‑101 (MQ=255)
                                    gtgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCGGCAG.TGTCA.GGTTTTCCA.GCGG.C.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                   <  1:3235548/99‑1 (MQ=2)
                                    ctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG..GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                   <  1:4104645/99‑1 (MQ=2)
                                    catcGTCGCTCCCCCCGCGGGGACGTGGATCGAATCCTATGAGGGCTGGCGGCCC..G.GTCGTTGAT.CCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                    <  2:3165394/98‑1 (MQ=0)
                                     tgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG.CG..GCGAACACCGATACCG.C.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                  >  1:2362919/2‑101 (MQ=2)
                                     agggTCGCCCCCCCCGGGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                 <  2:853172/98‑1 (MQ=255)
                                       cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                                               >  1:14741/1‑101 (MQ=255)
                                        gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgg                                                              >  2:3709900/1‑101 (MQ=255)
                                         ccGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGt                                                             <  1:1816451/100‑1 (MQ=255)
                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTggga                                                            >  2:3414626/1‑99 (MQ=255)
                                          cGCCCCCCCCGGGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTa                                                            <  1:2922167/101‑1 (MQ=255)
                                             tCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                                         >  2:1603454/1‑101 (MQ=255)
                                             tCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                                         >  2:1172760/1‑101 (MQ=255)
                                             cccccccGGGGGGGGGGGGATCAAAACACCTGCTTGTAGTGGG.CG..G.GCGGTCGGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                                         <  1:3873470/100‑1 (MQ=21)
                                                 cccGCGGGGGGGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCt                                                     <  1:2698946/101‑1 (MQ=255)
                                                 cccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCt                                                     <  2:3153209/101‑1 (MQ=255)
                                                   cgcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTgg                                                   >  1:2612570/1‑101 (MQ=255)
                                                   cgcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTgg                                                   <  2:2499199/101‑1 (MQ=255)
                                                    ggggggcCGTGAATCGAAACATCTGGTTGTGGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                                                  <  2:1048267/99‑1 (MQ=37)
                                                    gcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGGTCTGgc                                                  >  1:594519/1‑101 (MQ=255)
                                                      gggggCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgcgc                                                >  2:3200016/1‑101 (MQ=255)
                                                       ggggCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCa                                               >  2:2084684/1‑101 (MQ=255)
                                                        gggCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCaa                                              >  1:3001028/1‑101 (MQ=255)
                                                            gTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACgg                                          <  1:3185690/101‑1 (MQ=255)
                                                             tGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa                                         >  1:3518232/1‑101 (MQ=255)
                                                             gggATCGAACCATCTGCACGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa                                         <  2:1913152/100‑1 (MQ=255)
                                                              ggATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa                                        <  1:3069006/101‑1 (MQ=255)
                                                               gATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaaa                                       <  2:3930694/101‑1 (MQ=255)
                                                               gATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaaa                                       >  2:616699/1‑101 (MQ=255)
                                                               gATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAATGGTATCGGTCTGGCGCAAACGGaag                                       >  2:3092418/1‑100 (MQ=255)
                                                                  cGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTc                                    >  1:964882/1‑101 (MQ=255)
                                                                  cGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTc                                    >  2:554227/1‑101 (MQ=255)
                                                                  cGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCCTGGATCGAAACTGGTATGGATCTGGCGTGAACGGAAAGTc                                    >  1:1398615/1‑101 (MQ=255)
                                                                   gAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt                                   <  2:2609382/101‑1 (MQ=255)
                                                                   gAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt                                   <  2:4028524/101‑1 (MQ=255)
                                                                     aaCATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgt                                 <  2:1270110/101‑1 (MQ=255)
                                                                        aTCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgt                              <  2:591493/101‑1 (MQ=255)
                                                                         tCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgtc                             >  2:1643354/1‑101 (MQ=255)
                                                                         tCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgtc                             >  2:3507926/1‑101 (MQ=255)
                                                                          cTGCATGTAGGGGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGtcgtcg                            <  1:2951518/101‑1 (MQ=255)
                                                                            gCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCt                          >  2:1465308/1‑101 (MQ=255)
                                                                             cATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTc                         <  1:443112/101‑1 (MQ=255)
                                                                             cATGGAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTc                         <  1:4114201/101‑1 (MQ=255)
                                                                              gtGTGGTCGG.GG..G.GGAGTGGGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcc                        <  1:2382559/100‑1 (MQ=37)
                                                                              aTGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcc                        <  2:2686805/101‑1 (MQ=255)
                                                                                ttggTCGG.CG..G.CAAGTGGGA.GCGG.CAGTCGCTCCCCCCGCCGGGGCGGGGATCTAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcccc                      <  2:698051/98‑1 (MQ=25)
                                                                                gTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcccc                      >  1:179460/1‑101 (MQ=255)
                                                                                 tAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTccccc                     >  2:129530/1‑101 (MQ=255)
                                                                                  aGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcccccc                    >  2:4101757/1‑101 (MQ=255)
                                                                                     cgg.cg..g.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCcgcg                 >  1:275299/1‑101 (MQ=255)
                                                                                     cgg.cg..g.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCcgcg                 >  1:2390898/1‑101 (MQ=255)
                                                                                      gg.cg..g.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCgg                >  1:3280824/1‑101 (MQ=255)
                                                                                      gg.cg..g.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCgg                >  2:303445/1‑101 (MQ=255)
                                                                                      gg.cg..g.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCgg                >  1:336526/1‑101 (MQ=255)
                                                                                      gg.cg..g.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCgg                >  1:2788767/1‑101 (MQ=255)
                                                                                       g.cg..g.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCggg               >  1:3469124/1‑101 (MQ=255)
                                                                                          g..g.gCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCggggg             >  2:1402118/1‑101 (MQ=255)
                                                                                                cAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGt          >  1:2812043/1‑101 (MQ=255)
                                                                                                 aGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTg         <  1:1015688/101‑1 (MQ=255)
                                                                                                 aGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTg         >  1:56121/1‑101 (MQ=255)
                                                                                                 aGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTg         >  1:816544/1‑101 (MQ=255)
                                                                                                   ttCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGa       >  1:1496272/1‑101 (MQ=255)
                                                                                                   ttCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGa       >  2:2350300/1‑101 (MQ=255)
                                                                                                     cGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATc     <  1:511539/101‑1 (MQ=255)
                                                                                                     cGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATc     >  2:1885877/1‑101 (MQ=255)
                                                                                                      gA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCg    <  2:3242497/101‑1 (MQ=255)
                                                                                                      gA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCg    >  1:982636/1‑101 (MQ=255)
                                                                                                       a.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGa   <  1:2959564/101‑1 (MQ=255)
                                                                                                         gCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa  >  1:3583692/1‑101 (MQ=255)
                                                                                                         |                                                                                                     
CGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGG.CG..G.GCAGTTCGA.GCCG.CAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1395593‑1395793

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG