Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,743 G→C 17.2% intergenic (+555/‑376) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,7430G→C17.2% 112.6 ‑1.7 ‑0.0261intergenic (+555/‑376)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (18/27):  ref base (111/105):  total (129/132)
Fisher's exact test strand distribution p-value = 1.91e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 274 total aligned reads displayed.

CGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCC  >  NC_014034/1395644‑1395843
                                                                                                     |                                                                                                          
cgcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.g..g                                                                                                         >  1:2612570/1‑101 (MQ=255)
    ggggCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCa                                                                                                     >  2:2084684/1‑101 (MQ=255)
     gggCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCaa                                                                                                    >  1:3001028/1‑101 (MQ=255)
      ggCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCaaa                                                                                                   <  2:433997/101‑1 (MQ=255)
         gTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACgg                                                                                                <  1:3185690/101‑1 (MQ=255)
          tGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..a                                                                                             >  1:3518232/1‑101 (MQ=255)
           ggATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..aa                                                                                            <  1:3069006/101‑1 (MQ=255)
           ggATCGAAACAGCTGCATGTAGGCGGGGGCCAGTGCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..aa                                                                                            <  2:2476630/101‑1 (MQ=37)
            gATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..aaa                                                                                           >  2:616699/1‑101 (MQ=255)
            gATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAAT.GGTATCGGTCT.G..GCGCAAACGG..aag                                                                                           >  2:3092418/1‑100 (MQ=255)
               cGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCCTGGATCGAAACT.GGTATGGATCT.G..GCGTGAACGG..AAAGTc                                                                                        >  1:1398615/1‑101 (MQ=255)
                gAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCt                                                                                       >  1:2364363/1‑101 (MQ=255)
                gAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCt                                                                                       <  2:2609382/101‑1 (MQ=255)
                  aaCATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.g.t                                                                                   <  2:1270110/101‑1 (MQ=255)
                    cATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.g.tcg                                                                                 >  2:2236579/1‑101 (MQ=255)
                    cATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGCCT.g.tcg                                                                                 >  2:3220016/1‑101 (MQ=255)
                      tCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.g.gcgtc                                                                               >  1:919872/1‑101 (MQ=255)
                         gCATGTAGCCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCt                                                                            <  2:1876006/101‑1 (MQ=255)
                          cATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTc                                                                           <  1:443112/101‑1 (MQ=255)
                          cATGGAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTc                                                                           <  1:4114201/101‑1 (MQ=255)
                           aggTAGTCGGGGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcc                                                                          <  1:979659/99‑1 (MQ=255)
                           aTGTAGTCGGCGGGCATTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcc                                                                          <  1:3710154/101‑1 (MQ=255)
                           aTGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcc                                                                          <  2:2686805/101‑1 (MQ=255)
                              tAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTccccc                                                                       >  2:129530/1‑101 (MQ=255)
                                gTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCcg                                                                     >  1:1802356/1‑101 (MQ=255)
                                gTCGGCGGGCAGTCCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCcg                                                                     <  2:4030918/101‑1 (MQ=255)
                                  cggcggGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCcgcg                                                                   >  2:736160/1‑101 (MQ=255)
                                   ggcggGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCgg                                                                  >  1:336526/1‑101 (MQ=255)
                                     ggggCGGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCgggg                                                                <  1:1172854/100‑1 (MQ=255)
                                         cAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GTTATTGATCT.G..GCGCAAACGG..AAAGTCT.G.CCGTCGCTCCCCCCGCGGGGGCGt                                                            >  2:536582/1‑101 (MQ=255)
                                          aGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTg                                                           <  1:1015688/101‑1 (MQ=255)
                                           gtgcGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTgg                                                          <  1:2952267/98‑1 (MQ=255)
                                            ttCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGa                                                         >  1:1496272/1‑101 (MQ=255)
                                             tcaagattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTg                                                           >  1:3764702/9‑101 (MQ=2)
                                             tcaagattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTg                                                           >  1:611816/9‑101 (MQ=2)
                                              tgcgcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCA.GATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGa                                                         >  2:1027987/10‑101 (MQ=0)
                                              cGACCCGCAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                       <  2:2914618/101‑1 (MQ=255)
                                                cgcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCA.GATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                       <  1:3028020/94‑1 (MQ=0)
                                                agattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCGGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                        <  2:3673078/96‑1 (MQ=2)
                                                 gcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCA.GATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                      >  1:4151172/7‑101 (MQ=0)
                                                 gCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                    >  1:3583692/1‑101 (MQ=255)
                                                  attcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                      >  1:2833475/4‑101 (MQ=2)
                                                  attcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                      >  1:1998566/4‑101 (MQ=2)
                                                   ttcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                     <  2:697307/99‑1 (MQ=2)
                                                   gctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCA.GATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                    <  2:1485786/97‑1 (MQ=2)
                                                    gCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                 >  2:1757148/1‑101 (MQ=255)
                                                     tggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCA.GATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                  >  2:1907954/3‑101 (MQ=2)
                                                     cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCt                                                <  1:3412424/101‑1 (MQ=255)
                                                     cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCt                                                >  1:1129484/1‑101 (MQ=255)
                                                      ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCA.GATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                 <  1:1483899/100‑1 (MQ=2)
                                                      aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                  >  2:2128267/1‑101 (MQ=2)
                                                      aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTg                                               >  1:1573329/1‑101 (MQ=255)
                                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCG..CCGCTCTGGGT.AAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGGGGATCGAGAcaccc                                              >  1:693912/1‑97 (MQ=2)
                                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccc                                              <  1:566754/101‑5 (MQ=2)
                                                           cTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccg                                             <  2:2949159/101‑6 (MQ=2)
                                                           cTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccg                                             <  2:2343588/101‑6 (MQ=2)
                                                           cTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccg                                             <  2:3125093/101‑6 (MQ=2)
                                                             ccccccGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggc                                           >  1:4105905/1‑94 (MQ=0)
                                                             ccccccGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggc                                           >  2:3748858/1‑94 (MQ=0)
                                                             ccccccGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTg                                        >  1:2411606/1‑101 (MQ=255)
                                                             ccccccGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTg                                        <  2:3950416/101‑1 (MQ=255)
                                                             ccccccGCGGGGGCGTGGATCGAAACA.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTg                                        <  1:3550127/101‑1 (MQ=255)
                                                              cccccGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGa                                       <  2:816463/101‑1 (MQ=255)
                                                               ccccGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggcgc                                         <  1:1621800/101‑10 (MQ=2)
                                                                 ccGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGtt                                    >  1:3978081/1‑101 (MQ=255)
                                                                  cgcgGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTg                                   <  2:964791/101‑1 (MQ=255)
                                                                     gggggCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCa                                <  1:1967890/101‑1 (MQ=255)
                                                                      ggggCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAg                               <  1:1430911/101‑1 (MQ=255)
                                                                       gggCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGt                              >  1:2739811/1‑101 (MQ=255)
                                                                        ggCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTg                             >  2:1230011/1‑101 (MQ=255)
                                                                        ggCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTg                             >  2:3783139/1‑101 (MQ=255)
                                                                        ggCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTACGGCGTGAGTTGCCAGTg                             <  1:2195379/101‑1 (MQ=255)
                                                                         gCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGa                            <  2:982545/101‑1 (MQ=255)
                                                                           gTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATa                          <  2:1613234/101‑1 (MQ=255)
                                                                            tGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAg                         <  1:2475855/101‑1 (MQ=255)
                                                                             ggATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGa                        >  1:631523/1‑101 (MQ=255)
                                                                               aTCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACg                      >  1:2725732/1‑101 (MQ=255)
                                                                                tCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGc                     <  1:1892411/101‑1 (MQ=255)
                                                                                tCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGc                     >  2:3328829/1‑101 (MQ=255)
                                                                                tCGAAACC.TGTTTGGGTCT.G..GGGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGc                     <  1:2756509/101‑1 (MQ=37)
                                                                                 cGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGcc                    >  1:1645150/1‑101 (MQ=255)
                                                                                 cGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGcc                    >  1:368324/1‑101 (MQ=255)
                                                                                   aaaCT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGt                  >  2:2835321/1‑101 (MQ=255)
                                                                                   aaaCT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGt                  >  2:1083631/1‑101 (MQ=255)
                                                                                     aCT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGTCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTgg                <  2:1403057/101‑1 (MQ=255)
                                                                                      cT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGa               <  1:1757267/101‑1 (MQ=255)
                                                                                      cT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGa               >  2:3422542/1‑101 (MQ=255)
                                                                                      cT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGa               >  2:3592223/1‑101 (MQ=255)
                                                                                       t.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGAc              >  1:3450447/1‑101 (MQ=255)
                                                                                       t.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGAc              <  1:3200255/101‑1 (MQ=255)
                                                                                           tATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTc           <  2:3468872/101‑1 (MQ=255)
                                                                                            aTGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCg          <  2:275254/101‑1 (MQ=255)
                                                                                            aTGGATCT.G..GCGCAAACGG..AAAGGCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGCGAGTTGCCAGTGATAGACGCCGTGGACGTCg          >  2:298696/1‑101 (MQ=255)
                                                                                              ggATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCt        >  1:1877823/1‑101 (MQ=255)
                                                                                              ggATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCt        <  1:2084822/101‑1 (MQ=255)
                                                                                                aTCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCTCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTcc      >  1:1244178/1‑101 (MQ=255)
                                                                                                aTCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTcc      >  1:1306466/1‑101 (MQ=255)
                                                                                                aTCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTcc      >  2:1937197/1‑101 (MQ=255)
                                                                                                  cT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTcccc    <  1:875371/101‑1 (MQ=255)
                                                                                                     g..gCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTcccccc  >  1:170315/1‑101 (MQ=255)
                                                                                                     |                                                                                                          
CGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCC  >  NC_014034/1395644‑1395843

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG