Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,756 A→G 25.5% intergenic (+568/‑363) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,7560A→G25.5% 182.1 ‑1.6 0.0266intergenic (+568/‑363)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (29/39):  ref base (103/95):  total (132/134)
Fisher's exact test strand distribution p-value = 2.07e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 277 total aligned reads displayed.

GATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGA  >  NC_014034/1395656‑1395856
                                                                                                          |                                                                                                      
gATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..aaa                                                                                                        >  2:1187649/1‑101 (MQ=255)
    gAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCt                                                                                                    <  2:2609382/101‑1 (MQ=255)
         aTCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.g.tcgt                                                                                             <  2:591493/101‑1 (MQ=255)
           cTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.tcgtcg                                                                                           <  1:4113891/101‑1 (MQ=255)
               gtGTGGTCGGGGGGGAGTGGGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcc                                                                                       <  1:2382559/100‑1 (MQ=37)
               aTGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcc                                                                                       >  2:1290095/1‑101 (MQ=255)
                 ttggTCGGCGGCAAGTGGGAGCGGCAGTCGCTCCCCCCGCCGGGGCGGGGATCTAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcccc                                                                                     <  2:698051/98‑1 (MQ=25)
                 gTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcccc                                                                                     >  1:179460/1‑101 (MQ=255)
                   aGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcccccc                                                                                   >  2:4101757/1‑101 (MQ=255)
                      cggcggGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCcgcg                                                                                >  2:736160/1‑101 (MQ=255)
                       ggcggGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCgg                                                                               >  2:303445/1‑101 (MQ=255)
                       ggcggGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCgg                                                                               >  1:336526/1‑101 (MQ=255)
                       ggcggGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCgg                                                                               >  1:2788767/1‑101 (MQ=255)
                        gcggGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCggg                                                                              >  1:3469124/1‑101 (MQ=255)
                          gggCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCggggg                                                                            <  1:4122783/101‑1 (MQ=255)
                              aGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTg                                                                        >  1:56121/1‑101 (MQ=255)
                              aGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTg                                                                        <  1:1015688/101‑1 (MQ=255)
                                 tcaagattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTg                                                                        >  1:1894747/9‑101 (MQ=2)
                                  tgcgcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCA.GATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGa                                                                      >  2:1027987/10‑101 (MQ=0)
                                   gAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                   >  1:982636/1‑101 (MQ=255)
                                   gAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                   <  2:3242497/101‑1 (MQ=255)
                                   aagattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGa                                                                      >  2:3624839/7‑101 (MQ=2)
                                    agattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                     <  1:3818514/96‑1 (MQ=2)
                                    aGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                  <  1:2959564/101‑1 (MQ=255)
                                     gcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCA.GATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                   <  2:486512/95‑1 (MQ=0)
                                      attcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                   >  1:1998566/4‑101 (MQ=2)
                                       ttcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                  <  1:2451083/99‑1 (MQ=2)
                                       gctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCA.GATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                 <  1:4030793/97‑1 (MQ=2)
                                        tcAGTCCCGCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCGGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                 <  2:1266001/100‑1 (MQ=2)
                                         tggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCA.GATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                               >  2:1907954/3‑101 (MQ=2)
                                         cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                <  1:1148354/101‑1 (MQ=2)
                                         cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                <  1:2056282/101‑1 (MQ=2)
                                         cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCt                                                             >  2:647281/1‑101 (MQ=255)
                                          ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCA.GATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                              <  1:1483899/100‑1 (MQ=2)
                                          aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                               >  2:2128267/1‑101 (MQ=2)
                                          aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCta                                                            >  2:2195220/1‑100 (MQ=255)
                                          aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTg                                                            >  1:1573329/1‑101 (MQ=255)
                                          aGTCGCTCCCACCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTg                                                            >  1:3659981/1‑101 (MQ=255)
                                           gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGGCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                              >  1:1970189/1‑100 (MQ=2)
                                           gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgc                                                           <  2:3280583/101‑1 (MQ=255)
                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacc                                                            >  2:1769359/1‑98 (MQ=2)
                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacc                                                            <  1:425918/101‑4 (MQ=2)
                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGGGGATCGAAAcacc                                                            >  1:1236344/1‑98 (MQ=2)
                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCA.GATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtat                                                           <  1:1116532/101‑3 (MQ=2)
                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCG..CCGCTCTGGGT.AAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGGGGATCGAGAcaccc                                                           >  1:693912/1‑97 (MQ=2)
                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccc                                                           <  1:1525768/101‑5 (MQ=2)
                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccc                                                           <  2:2411731/101‑5 (MQ=2)
                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgcggc                                                        <  2:2890730/101‑1 (MQ=255)
                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCgcggg                                                        >  2:2319886/1‑100 (MQ=255)
                                               cTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccg                                                          <  2:3125093/101‑6 (MQ=2)
                                               cTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccg                                                          <  2:2343588/101‑6 (MQ=2)
                                               cTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCCTGGATCGAAAcacccg                                                          <  1:3695981/101‑6 (MQ=2)
                                                tCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCA.GATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatgag                                                        >  1:1389987/1‑96 (MQ=2)
                                                tCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCA.GATCCGCAATCGG..CCGGGCTCGATCGCCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatgag                                                        >  2:2816057/1‑96 (MQ=2)
                                                tCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgcggcgg                                                      >  2:1967764/1‑100 (MQ=255)
                                                 ccccccGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggc                                                        >  2:3748858/1‑94 (MQ=0)
                                                 ccccccGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggc                                                        <  1:3149155/101‑8 (MQ=0)
                                                 ccccccGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTg                                                     <  2:3950416/101‑1 (MQ=255)
                                                 ccccccGCGGGGGCGTGGATCGAAACA.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTg                                                     <  1:3550127/101‑1 (MQ=255)
                                                   ccccGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggcgc                                                      <  1:1621800/101‑10 (MQ=2)
                                                    cccGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggcgcc                                                     <  1:4098659/101‑11 (MQ=2)
                                                      cgcgGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTg                                                <  2:964791/101‑1 (MQ=255)
                                                        cgGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCATACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGcc                                              <  1:3572500/101‑1 (MQ=255)
                                                          ggggCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAg                                            <  1:1430911/101‑1 (MQ=255)
                                                           gggCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGt                                           >  1:2739811/1‑101 (MQ=255)
                                                           gggCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGt                                           >  2:2927724/1‑101 (MQ=255)
                                                            ggCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTg                                          >  2:2973250/1‑101 (MQ=255)
                                                            ggCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTg                                          >  2:3783139/1‑101 (MQ=255)
                                                            ggCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTACGGCGTGAGTTGCCAGTg                                          <  1:2195379/101‑1 (MQ=255)
                                                             gCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGa                                         <  2:982545/101‑1 (MQ=255)
                                                               gTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATa                                       <  2:1613234/101‑1 (MQ=255)
                                                               gTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATa                                       <  1:344289/101‑1 (MQ=255)
                                                                 ggATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGa                                     >  1:631523/1‑101 (MQ=255)
                                                                  gATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGAc                                    >  1:2993949/1‑101 (MQ=255)
                                                                   aTCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACg                                   >  1:2725732/1‑101 (MQ=255)
                                                                     cGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGcc                                 >  1:1645150/1‑101 (MQ=255)
                                                                     cGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGcc                                 <  1:952804/101‑1 (MQ=255)
                                                                       aaaCT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGt                               >  1:1717480/1‑101 (MQ=255)
                                                                        gaCT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTg                              >  2:1531239/2‑101 (MQ=255)
                                                                         aCT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGTCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTgg                             <  2:1403057/101‑1 (MQ=255)
                                                                          cT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGa                            >  2:3592223/1‑101 (MQ=255)
                                                                               tATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTc                        <  2:2737455/101‑1 (MQ=255)
                                                                               tATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTc                        <  2:890403/101‑1 (MQ=255)
                                                                               tATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTc                        <  1:2885532/101‑1 (MQ=255)
                                                                               tATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTc                        <  2:3468872/101‑1 (MQ=255)
                                                                                  ggATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCt                     >  1:1877823/1‑101 (MQ=255)
                                                                                   gATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTc                    <  1:303490/101‑1 (MQ=255)
                                                                                   gATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTc                    <  1:3742915/101‑1 (MQ=255)
                                                                                    aTCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTcc                   >  1:1306466/1‑101 (MQ=255)
                                                                                      cT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTcccc                 <  1:875371/101‑1 (MQ=255)
                                                                                       t.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTccccc                >  2:3401966/1‑101 (MQ=255)
                                                                                            gcgcAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCcg              <  2:1306370/101‑1 (MQ=255)
                                                                                              gcAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCcgcg            >  2:1799412/1‑101 (MQ=255)
                                                                                               cAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCgg           <  1:599329/101‑1 (MQ=255)
                                                                                                aaaCGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCggg          <  1:3402211/101‑1 (MQ=255)
                                                                                                   cGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGc       >  1:2588767/1‑101 (MQ=255)
                                                                                                   cGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGc       <  1:2329090/101‑1 (MQ=255)
                                                                                                    gg..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCg      >  1:3215379/1‑101 (MQ=255)
                                                                                                          aGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGa  >  1:1807410/1‑101 (MQ=255)
                                                                                                          aGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCCTGGa  >  1:2335903/1‑101 (MQ=255)
                                                                                                          |                                                                                                      
GATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGA  >  NC_014034/1395656‑1395856

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG