BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,395,763	C→G	18.0%	intergenic (+575/‑356)	RCAP_rcc01306 → / → RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,395,763	0	C→G	18.0%	112.2 ‑1.6 ‑0.0	257	intergenic (+575/‑356)	RCAP_rcc01306/RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (19/27): ref base (111/99): total (130/127)
Fisher's exact test strand distribution p-value = 1.93e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.98e-01

Only 100 of 287 total aligned reads displayed.


CATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC  >  NC_014034/1395664‑1395863


 aTCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.g.tcgt                                                                                                       >  1:3550556/1‑101 (MQ=255)


  tCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.g.tcgtc                                                                                                      >  2:3507926/1‑101 (MQ=255)


     gCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCt                                                                                                   >  2:1465308/1‑101 (MQ=255)


     gCATGTAGCCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCt                                                                                                   <  2:1876006/101‑1 (MQ=255)


       gtGTGGTCGGGGGGGAGTGGGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcc                                                                                                 <  1:2382559/100‑1 (MQ=37)


       aggTAGTCGGGGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcc                                                                                                 <  1:979659/99‑1 (MQ=255)


       aggTAGTCGGGGGGCAGTGCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcc                                                                                                 <  1:1494179/99‑1 (MQ=255)


       aTGTAGTCGGCGGGCATTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcc                                                                                                 <  1:3710154/101‑1 (MQ=255)


       aTGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcc                                                                                                 <  2:2686805/101‑1 (MQ=255)


         ttggTCGGCGGCAAGTGGGAGCGGCAGTCGCTCCCCCCGCCGGGGCGGGGATCTAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcccc                                                                                               <  2:698051/98‑1 (MQ=25)


             gcggcggGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCcgc                                                                                           <  2:3620668/100‑1 (MQ=255)


               ggcggGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCgg                                                                                         >  1:2788767/1‑101 (MQ=255)


                 ggggCGGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCgggg                                                                                       <  1:1172854/100‑1 (MQ=255)


                  gggCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCggggg                                                                                      <  1:4122783/101‑1 (MQ=255)


                   ggCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGc                                                                                     <  2:1327868/101‑1 (MQ=255)


                     cAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GTTATTGATCT.G..GCGCAAACGG..AAAGTCT.G.CCGTCGCTCCCCCCGCGGGGGCGt                                                                                   >  2:536582/1‑101 (MQ=255)


                      aGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTg                                                                                  <  1:1015688/101‑1 (MQ=255)


                      aGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTg                                                                                  >  1:816544/1‑101 (MQ=255)


                      aGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTg                                                                                  >  1:56121/1‑101 (MQ=255)


                          tgcgcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCA.GATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGa                                                                                >  2:1027987/10‑101 (MQ=0)


                           aagattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGa                                                                                >  1:1143607/7‑101 (MQ=2)


                            agattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                               <  1:3818514/96‑1 (MQ=2)


                            agattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCGGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                               <  2:3673078/96‑1 (MQ=2)


                            aGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                            <  1:2959564/101‑1 (MQ=255)


                             gattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                              >  2:4109434/5‑101 (MQ=2)


                             gCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                           >  1:3583692/1‑101 (MQ=255)


                               gctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCA.GATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                           <  1:4030793/97‑1 (MQ=2)


                               cGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                         >  1:1322548/1‑101 (MQ=255)


                                 cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                          <  1:1991216/101‑1 (MQ=2)


                                 cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCt                                                                       >  2:647281/1‑101 (MQ=255)


                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCA.GATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCta                                                                      >  2:1279990/1‑100 (MQ=2)


                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacc                                                                      <  1:425918/101‑4 (MQ=2)


                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacc                                                                      >  2:1769359/1‑98 (MQ=2)


                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATCG..CCGCTCTGGGT.AAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGGGGATCGAGAcaccc                                                                     >  1:693912/1‑97 (MQ=2)


                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccc                                                                     <  1:1458442/101‑5 (MQ=2)


                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgcg.gc                                                                 <  2:2890730/101‑1 (MQ=255)


                                       cTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccg                                                                    <  2:3125093/101‑6 (MQ=2)


                                       cTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCA.GATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatga                                                                   <  2:21273/101‑5 (MQ=2)


                                       cTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCA.GATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatga                                                                   >  1:2638677/1‑97 (MQ=2)


                                        tCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccgg                                                                   >  1:3393258/1‑95 (MQ=2)


                                        tCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCA.GATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatgag                                                                  >  1:1633711/1‑96 (MQ=2)


                                        tCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgcg.gcgg                                                               >  2:1967764/1‑100 (MQ=255)


                                        tCCCCCCGCGGGGGCGTAGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccgg                                                                   <  1:3505597/101‑7 (MQ=2)


                                         ccccccGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggc                                                                  <  1:3149155/101‑8 (MQ=0)


                                         ccccccGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTg                                                              <  2:3950416/101‑1 (MQ=255)


                                         ccccccGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTg                                                              >  1:2411606/1‑101 (MQ=255)


                                          cccccGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggcg                                                                 >  2:2829191/1‑93 (MQ=2)


                                            cccGCGGGGGCGTGGATCGAAACTCGGCAAGGACAT.C..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggcgcc                                                               <  1:4109695/101‑11 (MQ=2)


                                              cgcgGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTg                                                         <  2:3055663/101‑1 (MQ=255)


                                              cgcgGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTg                                                         <  2:964791/101‑1 (MQ=255)


                                               gcgGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGc                                                        >  1:2118968/1‑101 (MQ=255)


                                                   gggCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGt                                                    >  2:2927724/1‑101 (MQ=255)


                                                   gggCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGt                                                    >  1:2739811/1‑101 (MQ=255)


                                                    ggCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTg                                                   >  2:1230011/1‑101 (MQ=255)


                                                     gCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGa                                                  <  2:982545/101‑1 (MQ=255)


                                                     gCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTGGCCAGTGa                                                  <  1:3414871/101‑1 (MQ=255)


                                                       gTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATa                                                <  2:1613234/101‑1 (MQ=255)


                                                         ggATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGa                                              >  1:631523/1‑101 (MQ=255)


                                                          gATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.c                                            >  1:2993949/1‑101 (MQ=255)


                                                            tCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGc                                          >  2:3328829/1‑101 (MQ=255)


                                                            tCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGc                                          <  1:1892411/101‑1 (MQ=255)


                                                             cGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGcc                                         >  1:368324/1‑101 (MQ=255)


                                                              gAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCg                                        >  1:2516226/1‑101 (MQ=255)


                                                                gaCT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTg                                      >  2:1531239/2‑101 (MQ=255)


                                                                  cT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGa                                    <  1:22412/101‑1 (MQ=255)


                                                                  cT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGa                                    >  2:3422542/1‑101 (MQ=255)


                                                                  cT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGa                                    <  1:1757267/101‑1 (MQ=255)


                                                                   t.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.c                                  <  2:2741296/101‑1 (MQ=255)


                                                                     ggTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.Cg                                 <  1:1561851/101‑1 (MQ=255)


                                                                       tATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTc                               <  2:890403/101‑1 (MQ=255)


                                                                       tATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTc                               <  1:2885532/101‑1 (MQ=255)


                                                                        aTGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCg                              >  1:1592404/1‑101 (MQ=255)


                                                                        aTGGATCT.G..GCGCAAACGG..AAAGGCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGCGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCg                              >  2:298696/1‑101 (MQ=255)


                                                                          ggATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCt                            >  1:1877823/1‑101 (MQ=255)


                                                                           gATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTc                           <  1:303490/101‑1 (MQ=255)


                                                                            aTCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCTCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTcc                          >  1:1244178/1‑101 (MQ=255)


                                                                              cT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTcccc                        <  1:1424778/101‑1 (MQ=255)


                                                                                 g..gCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTcccccc                      >  1:170315/1‑101 (MQ=255)


                                                                                    gcgcAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCcg                     <  2:1306370/101‑1 (MQ=255)


                                                                                     cgcAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCcgc                    >  1:1110315/1‑101 (MQ=255)


                                                                                      gcAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCcgcg                   >  1:3468910/1‑101 (MQ=255)


                                                                                       cAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCgg                  <  1:599329/101‑1 (MQ=255)


                                                                                       cAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCgg                  >  1:2032776/1‑101 (MQ=255)


                                                                                       cAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCgg                  >  2:2193587/1‑101 (MQ=255)


                                                                                       cAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCgg                  >  2:3767055/1‑101 (MQ=255)


                                                                                        aaaCGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCggg                 >  1:4081920/1‑101 (MQ=255)


                                                                                         aaCGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCgggg                >  2:2340408/1‑101 (MQ=255)


                                                                                         aaCGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCgggg                >  2:3250835/1‑101 (MQ=255)


                                                                                          aCGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGCAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCggggg               <  2:2411430/101‑1 (MQ=255)


                                                                                             g..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGt            <  1:2330874/101‑1 (MQ=255)


                                                                                                 aaGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTgg          >  2:2266842/1‑101 (MQ=255)


                                                                                                  ggacccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA.CTGCGTGCGCGCCATG.AATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCGt            <  1:1912590/92‑1 (MQ=0)


                                                                                                  ggacccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA.CTGCGTGCGCGCCATG.AATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCGt            >  2:1918783/10‑101 (MQ=0)


                                                                                                  aGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGa         >  1:1807410/1‑101 (MQ=255)


                                                                                                    tCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATc       >  1:3213383/1‑101 (MQ=255)


                                                                                                     cT.G.TCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCg      <  1:901282/101‑1 (MQ=255)


                                                                                                        g.tcgtcGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa    >  1:3900193/1‑101 (MQ=255)


                                                                                                        g.tcgtcGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa    <  1:3329074/101‑1 (MQ=255)


                                                                                                          tcgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa   >  2:2453383/1‑101 (MQ=255)


                                                                                                           cgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc  <  2:772701/101‑1 (MQ=255)


CATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCT.G..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC  >  NC_014034/1395664‑1395863

Base quality scores:


ATCG

< 3 ≤


ATCG

< 22 ≤


ATCG

< 30 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG