Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,806 A→C 4.9% intergenic (+618/‑313) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,8060A→C 4.9% 18.2 ‑0.3 ‑1.3204intergenic (+618/‑313)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/4):  ref base (97/97):  total (103/101)
Fisher's exact test strand distribution p-value = 7.48e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.57e-02

Only 100 of 211 total aligned reads displayed.

CCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCC  >  NC_014034/1395706‑1395906
                                                                                                      |                                                                                                       
   ccGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGtt                                                                                                      >  1:3978081/1‑101 (MQ=255)
    cgcgGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTg                                                                                                     <  2:3055663/101‑1 (MQ=255)
     gcgGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGc                                                                                                    <  1:578812/101‑1 (MQ=255)
     gcgGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGc                                                                                                    >  1:2118968/1‑101 (MQ=255)
     gcgGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCACCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGc                                                                                                    <  1:1909009/101‑1 (MQ=255)
       gagggCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAATTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCa                                                                                                  <  1:187084/99‑1 (MQ=255)
        ggggCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAg                                                                                                 <  1:1430911/101‑1 (MQ=255)
         gggCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGt                                                                                                >  1:2739811/1‑101 (MQ=255)
          ggCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTACG.GCGTGAGTTGCCAGTg                                                                                               <  1:2195379/101‑1 (MQ=255)
            cGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGTTCGAAAC.CTGCG.GCGTGAGTTGCCAGGGAt                                                                                             >  1:1653899/1‑101 (MQ=255)
            cGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGGG.GCGTGAGTTGCCAGTGAt                                                                                             >  2:721981/1‑101 (MQ=255)
             gTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATa                                                                                            <  2:1613234/101‑1 (MQ=255)
             gTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATa                                                                                            <  1:344289/101‑1 (MQ=255)
             gTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATa                                                                                            <  1:1885997/101‑1 (MQ=255)
              tGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAg                                                                                           <  1:2475855/101‑1 (MQ=255)
               ggATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGa                                                                                          <  1:2350468/101‑1 (MQ=255)
                gATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.c                                                                                        >  2:806233/1‑101 (MQ=255)
                gATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.c                                                                                        >  1:2993949/1‑101 (MQ=255)
                 aTCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.g                                                                                      <  2:636854/101‑1 (MQ=255)
                  tCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.Gc                                                                                     >  2:3328829/1‑101 (MQ=255)
                  tCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.Gc                                                                                     <  1:366117/101‑1 (MQ=255)
                  tCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.Gc                                                                                     >  1:3191098/1‑101 (MQ=255)
                   cGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.Gcc                                                                                    <  1:952804/101‑1 (MQ=255)
                     aaaCTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGt                                                                                  >  1:1717480/1‑101 (MQ=255)
                      gaCTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTg                                                                                 >  2:1531239/2‑101 (MQ=255)
                       aCTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGTCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTgg                                                                                <  2:1403057/101‑1 (MQ=255)
                        cTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGa                                                                               <  1:1757267/101‑1 (MQ=255)
                         tGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.c                                                                             <  2:2741296/101‑1 (MQ=255)
                            tATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTc                                                                          <  2:3468872/101‑1 (MQ=255)
                            tATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTc                                                                          <  1:2885532/101‑1 (MQ=255)
                             aTGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCg                                                                         >  1:1592404/1‑101 (MQ=255)
                             aTGGATCTGGCGCAAACGGAAAGGCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGCGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCg                                                                         >  2:298696/1‑101 (MQ=255)
                              tGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGc                                                                        <  2:965906/101‑1 (MQ=255)
                                gATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTc                                                                      <  1:303490/101‑1 (MQ=255)
                                gATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTc                                                                      <  1:3742915/101‑1 (MQ=255)
                                 aTCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTcc                                                                     >  2:1937197/1‑101 (MQ=255)
                                 aTCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTcc                                                                     >  1:1306466/1‑101 (MQ=255)
                                   cTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTcccc                                                                   <  1:875371/101‑1 (MQ=255)
                                    tGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTccccc                                                                  <  1:616763/101‑1 (MQ=255)
                                    tGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTccccc                                                                  >  2:3401966/1‑101 (MQ=255)
                                     ggCGCAAACGGAAAGTCTGTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTcccccc                                                                 <  1:3389196/101‑1 (MQ=255)
                                      gcgcAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCcg                                                                <  2:1306370/101‑1 (MQ=255)
                                        gcAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCcgcg                                                              >  1:3468910/1‑101 (MQ=255)
                                        gcAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCcgcg                                                              >  2:1799412/1‑101 (MQ=255)
                                         cAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCgg                                                             >  1:2032776/1‑101 (MQ=255)
                                         cAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCgg                                                             >  2:3767055/1‑101 (MQ=255)
                                          aaaCGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCggg                                                            >  1:4081920/1‑101 (MQ=255)
                                           aaCGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCgggg                                                           >  2:3250835/1‑101 (MQ=255)
                                           aaCGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCgggg                                                           <  1:2236738/101‑1 (MQ=255)
                                           aaCGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCgggg                                                           >  2:2340408/1‑101 (MQ=255)
                                             cGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGc                                                         >  1:358168/1‑101 (MQ=255)
                                               gAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGt                                                       <  1:2330874/101‑1 (MQ=255)
                                                ggacccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA..CTGCGTGCGCGCCATG.AATTCATCGAGC.GCCATCGAGCGTCGCTCCCCCCGCGGGGGCGt                                                       >  2:3633167/10‑101 (MQ=0)
                                                ggacccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA..CTGCGTGCGCGCCATG.AATTCATCGAGC.GCCATCGAGCGTCGCTCCCCCCGCGGGGGCGt                                                       >  1:1044080/10‑101 (MQ=0)
                                                aaaGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTg                                                      <  2:919787/101‑1 (MQ=255)
                                                 gacccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA..CTGCGTGCGCGCCATG.AATTCATCGAGC.GCCATCGAGCGTCGCTCCCCCCGCGGGGGCGTg                                                      <  1:2295285/93‑1 (MQ=0)
                                                 aaGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTgg                                                     >  2:2266842/1‑101 (MQ=255)
                                                   cccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA..CTGCGTGCGCGCCATG.AATTCATCGAGC.GCCATCGAGCGTCGCTCCCCCCGCGGCGGCGTggc                                                    >  2:2853126/7‑100 (MQ=0)
                                                    tCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                  >  1:3213383/1‑101 (MQ=255)
                                                     cTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                 >  2:3122353/1‑101 (MQ=255)
                                                     cTGTCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                 <  1:901282/101‑1 (MQ=255)
                                                      tGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                <  1:7077/101‑1 (MQ=255)
                                                       gtcgtcGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                               >  1:3900193/1‑101 (MQ=255)
                                                       ggcgtcgCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                               <  1:1159112/99‑1 (MQ=255)
                                                        tcgtcgCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                              >  2:2453383/1‑101 (MQ=255)
                                                         cgtcgCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                             <  2:14730/101‑1 (MQ=255)
                                                           tcgCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                           >  1:3410438/1‑101 (MQ=255)
                                                             gCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCTgcg                                         >  2:1239515/1‑101 (MQ=255)
                                                                ccccccGCGGGGGCGTGGATCGAAACAATGCG.GCGCGA...GCCTATGGCTGA.CGGACCCGCC.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgcgtgcg                                     <  1:3571313/101‑5 (MQ=2)
                                                                ccccccGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgccg                                      <  1:3422787/101‑1 (MQ=255)
                                                                   cccGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTCAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCt                                   <  2:1107604/101‑1 (MQ=255)
                                                                    ccGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTa                                  >  1:2000319/1‑101 (MQ=255)
                                                                       cgGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCg                               >  1:1239995/1‑101 (MQ=255)
                                                                        gggggCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGc                              >  1:1475076/1‑101 (MQ=255)
                                                                         ggggCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGcc                             >  1:2700882/1‑100 (MQ=255)
                                                                          gggCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCAt                            >  1:2542545/1‑101 (MQ=255)
                                                                          gggCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCAt                            >  2:479063/1‑101 (MQ=255)
                                                                           ggCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATc                           >  1:1457696/1‑101 (MQ=255)
                                                                           ggCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATc                           <  1:1465410/101‑1 (MQ=255)
                                                                              gTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCgg                        >  2:2396631/1‑101 (MQ=255)
                                                                              gTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCgg                        <  1:1643469/101‑1 (MQ=255)
                                                                               tGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGt                       >  1:510830/1‑101 (MQ=255)
                                                                               tGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGt                       >  1:1266308/1‑101 (MQ=255)
                                                                                  aTCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGa                    >  2:2725884/1‑101 (MQ=255)
                                                                                   tCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAg                   <  2:2415952/101‑1 (MQ=255)
                                                                                      aaaC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAgcgc                >  1:1354526/1‑101 (MQ=255)
                                                                                      aaaC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAgcgc                >  2:1770907/1‑101 (MQ=255)
                                                                                       aaC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGcgcc               >  2:403740/1‑101 (MQ=255)
                                                                                        aC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGcgccg              <  2:2612379/101‑1 (MQ=255)
                                                                                             gcg.gcgTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCa          <  2:3110607/101‑1 (MQ=255)
                                                                                              cg.gcgTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAg         <  1:1925214/101‑1 (MQ=255)
                                                                                              cg.gcgTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAg         <  1:536646/101‑1 (MQ=255)
                                                                                               g.gcgTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGt        >  2:927227/1‑101 (MQ=255)
                                                                                                 gcgTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGGCGCTCCCCCCGCGGGGGCGTGAATCGAAACTGCGCCGCCTTTCGCATCCGGTCGAGCGCCGCGCAGTc       <  2:3848802/101‑1 (MQ=255)
                                                                                                  ggTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCg      <  2:2811824/100‑1 (MQ=255)
                                                                                                   gTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGc     <  1:3251076/101‑1 (MQ=255)
                                                                                                    tGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCCGTCGcc    >  2:863440/1‑100 (MQ=255)
                                                                                                    tGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGcc    >  2:2183684/1‑100 (MQ=255)
                                                                                                     gAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTc   <  2:2966121/101‑1 (MQ=255)
                                                                                                      aGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTcc  <  2:3013577/101‑1 (MQ=255)
                                                                                                      |                                                                                                       
CCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCC  >  NC_014034/1395706‑1395906

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG