Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,828 G→C 5.3% intergenic (+640/‑291) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,8280G→C 5.3% 16.5 ‑0.3 ‑1.0188intergenic (+640/‑291)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/4):  ref base (91/87):  total (97/91)
Fisher's exact test strand distribution p-value = 7.48e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.90e-02

Only 100 of 196 total aligned reads displayed.

AACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGA  >  NC_014034/1395728‑1395927
                                                                                                        |                                                                                                    
gaCTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTg                                                                                                      >  2:1531239/2‑101 (MQ=255)
  cTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGa                                                                                                    >  2:3422542/1‑101 (MQ=255)
  cTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGa                                                                                                    <  1:22412/101‑1 (MQ=255)
  cTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGa                                                                                                    <  1:1757267/101‑1 (MQ=255)
   tGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.c                                                                                                  <  2:2741296/101‑1 (MQ=255)
      tATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTc                                                                                               <  2:890403/101‑1 (MQ=255)
       aTGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCg                                                                                              >  1:1592404/1‑101 (MQ=255)
       aTGGATCTGGCGCAAACGGAAAGGCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGCGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCg                                                                                              >  2:298696/1‑101 (MQ=255)
        tGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGc                                                                                             <  2:965906/101‑1 (MQ=255)
         ggATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCt                                                                                            <  1:2084822/101‑1 (MQ=255)
          gATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTc                                                                                           <  1:303490/101‑1 (MQ=255)
           aTCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTcc                                                                                          >  2:1937197/1‑101 (MQ=255)
             cTGGCGCAAACGGAAAGTCTGTCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTcccc                                                                                        <  1:1424778/101‑1 (MQ=255)
               ggCGCAAACGGAAAGTCTGTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTcccccc                                                                                      <  1:3389196/101‑1 (MQ=255)
                  gcAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCcgcg                                                                                   >  1:3468910/1‑101 (MQ=255)
                  gcAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCcgcg                                                                                   >  2:1799412/1‑101 (MQ=255)
                   cAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCgg                                                                                  <  1:599329/101‑1 (MQ=255)
                   cAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCgg                                                                                  >  1:2032776/1‑101 (MQ=255)
                   cAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCgg                                                                                  >  2:3023044/1‑101 (MQ=255)
                   cAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCgg                                                                                  >  2:3767055/1‑101 (MQ=255)
                    aaaCGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCggg                                                                                 >  1:4081920/1‑101 (MQ=255)
                       cGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGc                                                                              <  1:2329090/101‑1 (MQ=255)
                       cGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGc                                                                              >  1:2588767/1‑101 (MQ=255)
                         gAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCgg                                                                            >  2:691232/1‑100 (MQ=255)
                          ggacccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA..CTGCGTGCGCGCCATG.AATTCATCGAGC.GCCATCGAGCGTCGCTCCCCCCGCGGGGGCGt                                                                            >  2:1325824/10‑101 (MQ=0)
                          ggacccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA..CTGCGTGCGCGCCATG.AATTCATCGAGC.GCCATCGAGCGTCGCTCCCCCCGCGGGGGCGt                                                                            >  2:3633167/10‑101 (MQ=0)
                          ggacccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA..CTGCGTGCGCGCCATG.AATTCATCGAGC.GCCATCGAGCGTCGCTCCCCCCGCGGGGGCGt                                                                            >  1:1044080/10‑101 (MQ=0)
                          ggacccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA..CTGCGTGCGCGCCATG.AATTCATCGAGC.GCCATCGAGCGTCGCTCCCCCCGCGGGGGCGt                                                                            <  1:1912590/92‑1 (MQ=0)
                          aaaGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTg                                                                           <  2:919787/101‑1 (MQ=255)
                           gacccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA..CTGCGTGCGCGCCATG.AATTCATCGAGC.GCCATCGAGCGTCGCTCCCCCCGCGGGGGCGTg                                                                           <  1:3922852/93‑1 (MQ=0)
                           aaGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTgg                                                                          <  1:298741/101‑1 (MQ=255)
                           aaGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTgg                                                                          >  2:2266842/1‑101 (MQ=255)
                            aGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                                         >  1:1807410/1‑101 (MQ=255)
                             gTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                        >  1:3853416/1‑101 (MQ=255)
                              tCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                       >  1:3213383/1‑101 (MQ=255)
                               cTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      >  2:3122353/1‑101 (MQ=255)
                                tGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                     <  1:7077/101‑1 (MQ=255)
                                tGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                     <  1:1345908/101‑1 (MQ=255)
                                 gtcgtcGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    <  1:3329074/101‑1 (MQ=255)
                                 ggcgtcgCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    <  1:1159112/99‑1 (MQ=255)
                                  tcgtcgCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                   >  2:2453383/1‑101 (MQ=255)
                                   cgtcgCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                  <  2:14730/101‑1 (MQ=255)
                                    gtcgCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                                 <  1:3429159/101‑1 (MQ=255)
                                     tcgCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                                                >  1:3410438/1‑101 (MQ=255)
                                     tcgCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                                                >  1:2397009/1‑101 (MQ=255)
                                      cgCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgc                                                               >  2:4061945/1‑101 (MQ=255)
                                          ccccccGCGGGGGCGTGGATCGAAACAATGCG.GCGCGA...GCCTATGGCTGA.CGGACCCGCC.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgcgtgcg                                                          <  1:3571313/101‑5 (MQ=2)
                                             cccGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCt                                                        >  2:1256389/1‑101 (MQ=255)
                                             cccGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTCAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCt                                                        <  2:1107604/101‑1 (MQ=255)
                                              ccGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTa                                                       >  1:2000319/1‑101 (MQ=255)
                                              ccGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTa                                                       >  1:928397/1‑101 (MQ=255)
                                                 cgGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCg                                                    >  1:1239995/1‑101 (MQ=255)
                                                 cgGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCg                                                    >  2:4024202/1‑101 (MQ=255)
                                                   ggggCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGcc                                                  >  1:2700882/1‑100 (MQ=255)
                                                    gggCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCAt                                                 >  1:2542545/1‑101 (MQ=255)
                                                    gggCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCAt                                                 >  2:479063/1‑101 (MQ=255)
                                                    gggCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCAt                                                 >  2:3288612/1‑101 (MQ=255)
                                                     ggCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATc                                                >  1:1457696/1‑101 (MQ=255)
                                                     ggCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATc                                                <  2:2549645/101‑1 (MQ=255)
                                                        gTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCgg                                             >  2:2396631/1‑101 (MQ=255)
                                                        gTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCgg                                             <  1:1643469/101‑1 (MQ=255)
                                                         tGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTAtagatcggaag                                            >  2:3091886/1‑91 (MQ=255)
                                                         tGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGt                                            >  1:510830/1‑101 (MQ=255)
                                                         tGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGt                                            >  1:1266308/1‑101 (MQ=255)
                                                            aTCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGa                                         >  2:2725884/1‑101 (MQ=255)
                                                             tCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAg                                        <  2:2415952/101‑1 (MQ=255)
                                                               gAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAgcg                                      >  2:1632337/1‑101 (MQ=255)
                                                                aaaC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAgcgc                                     >  2:1770907/1‑101 (MQ=255)
                                                                 aaC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGcgcc                                    <  2:2364187/101‑1 (MQ=255)
                                                                  aC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGcgccg                                   <  2:2612379/101‑1 (MQ=255)
                                                                       gcg.gcgTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCa                               <  2:1406628/101‑1 (MQ=255)
                                                                        cg.gcgTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAg                              <  1:536646/101‑1 (MQ=255)
                                                                        cg.gcgTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAg                              <  1:2204414/101‑1 (MQ=255)
                                                                         g.gcgTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGt                             >  2:927227/1‑101 (MQ=255)
                                                                           gcgTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGGCGCTCCCCCCGCGGGGGCGTGAATCGAAACTGCGCCGCCTTTCGCATCCGGTCGAGCGCCGCGCAGTc                            <  2:3848802/101‑1 (MQ=255)
                                                                            cgTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCg                           >  1:1466493/1‑101 (MQ=255)
                                                                             gTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGc                          <  1:3251076/101‑1 (MQ=255)
                                                                              tGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGcc                         >  2:2183684/1‑100 (MQ=255)
                                                                              tGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCt                         <  1:1531343/101‑1 (MQ=255)
                                                                               gAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTc                        <  2:2966121/101‑1 (MQ=255)
                                                                                aGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTcc                       <  1:896238/101‑1 (MQ=255)
                                                                                  ttGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTcccc                     >  1:1105340/1‑101 (MQ=255)
                                                                                  ttGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTcccc                     >  2:560565/1‑101 (MQ=255)
                                                                                  ttGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGATcccc                     >  2:1357098/1‑101 (MQ=255)
                                                                                  gtGCCAGTGATAGA.C.GCCGTGGG.CGTCGCTGCCCCCGCGGGCGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTcccc                     <  2:323376/100‑1 (MQ=255)
                                                                                    gCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTcccccc                   <  2:631459/101‑1 (MQ=255)
                                                                                      cAGTGATAGA.C.GCCGGGGA.CGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCcgc                 <  1:3174793/101‑1 (MQ=255)
                                                                                       aGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCcgcg                <  1:1937323/101‑1 (MQ=255)
                                                                                        gTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCgg               >  2:3587048/1‑101 (MQ=255)
                                                                                            tAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGc           >  1:3736246/1‑101 (MQ=255)
                                                                                            tAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGc           >  2:2841934/1‑101 (MQ=255)
                                                                                             aGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCg          >  2:39093/1‑101 (MQ=255)
                                                                                             aGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCg          >  2:77838/1‑101 (MQ=255)
                                                                                               a.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTg        <  2:1807291/101‑1 (MQ=255)
                                                                                               a.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTg        >  1:2334848/1‑101 (MQ=255)
                                                                                                    ccGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGAt     <  1:736236/101‑1 (MQ=255)
                                                                                                    ccGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGAt     <  2:2788548/101‑1 (MQ=255)
                                                                                                     cGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATc    <  2:3001493/101‑1 (MQ=255)
                                                                                                      gTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCg   >  1:2813890/1‑101 (MQ=255)
                                                                                                      gTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGACGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCg   >  1:3631782/1‑101 (MQ=255)
                                                                                                        |                                                                                                    
AACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCG.GCGTGAGTTGCCAGTGATAGA.C.GCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGA  >  NC_014034/1395728‑1395927

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG