Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,839 C→A 8.6% intergenic (+651/‑280) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,8390C→A 8.6% 25.5 ‑1.1 ‑0.3205intergenic (+651/‑280)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/11):  ref base (99/88):  total (106/99)
Fisher's exact test strand distribution p-value = 3.25e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.96e-01

Only 100 of 231 total aligned reads displayed.

ATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTC  >  NC_014034/1395739‑1395939
                                                                                                       |                                                                                                            
   tGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTccccc                                                                                                           >  2:3401966/1‑101 (MQ=255)
    ggCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTcccccc                                                                                                          >  1:170315/1‑101 (MQ=255)
       gcAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCcgcg                                                                                                       >  2:1799412/1‑101 (MQ=255)
        cAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCgg                                                                                                      >  2:3023044/1‑101 (MQ=255)
        cAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCgg                                                                                                      <  1:599329/101‑1 (MQ=255)
        cAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCgg                                                                                                      >  2:3767055/1‑101 (MQ=255)
        cAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCgg                                                                                                      >  1:2032776/1‑101 (MQ=255)
         aaaCGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCggg                                                                                                     >  1:4078910/1‑101 (MQ=255)
          aaCGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCgggg                                                                                                    >  2:3250835/1‑101 (MQ=255)
               ggacccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA.CTGCGTGCGCGCCATG.AATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCGt                                                                                                >  2:1325824/10‑101 (MQ=0)
               ggacccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA.CTGCGTGCGCGCCATG.AATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCGt                                                                                                >  2:1918783/10‑101 (MQ=0)
               ggacccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA.CTGCGTGCGCGCCATG.AATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCGt                                                                                                <  1:1912590/92‑1 (MQ=0)
               aaaGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTg                                                                                               <  2:919787/101‑1 (MQ=255)
                gacccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA.CTGCGTGCGCGCCATG.AATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCGTg                                                                                               <  1:2295285/93‑1 (MQ=0)
                aaGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTgg                                                                                              >  2:2266842/1‑101 (MQ=255)
                  cccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA.CTGCGTGCGCGCCATG.AATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGCGGCGTggc                                                                                             >  2:2853126/7‑100 (MQ=0)
                     tGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                                         <  1:7077/101‑1 (MQ=255)
                     tGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                                         <  1:1345908/101‑1 (MQ=255)
                      gtcgtcGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                                        >  1:3900193/1‑101 (MQ=255)
                      gtcgtcGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                                        <  1:3329074/101‑1 (MQ=255)
                      ggcgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                                        <  1:1159112/99‑1 (MQ=255)
                        cgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                                      <  2:14730/101‑1 (MQ=255)
                        cgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                                      <  2:772701/101‑1 (MQ=255)
                         gtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                                                     <  1:3429159/101‑1 (MQ=255)
                          tcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                                                                    >  1:3410438/1‑101 (MQ=255)
                          tcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                                                                    >  1:2397009/1‑101 (MQ=255)
                            gCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCTgcg                                                                                  >  2:1239515/1‑101 (MQ=255)
                            gCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgcg                                                                                  <  1:3508179/101‑1 (MQ=255)
                            gCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGGGGA.GGTCGCTCCCCCCCCGGGGGGGTGGATCGAAACGgcg                                                                                  >  1:517506/1‑101 (MQ=37)
                                  cccGCGGGGGCGTGGATCGAAACCTGCG.GCGTCAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.Ct                                                                          <  2:1107604/101‑1 (MQ=255)
                                   ccGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTa                                                                         >  1:2000319/1‑101 (MQ=255)
                                      cgGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCg                                                                      >  2:4024202/1‑101 (MQ=255)
                                      cgGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCg                                                                      >  1:1239995/1‑101 (MQ=255)
                                        ggggCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGcc                                                                    >  1:2700882/1‑100 (MQ=255)
                                         gggCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCAt                                                                   >  2:479063/1‑101 (MQ=255)
                                         gggCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGCCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCAt                                                                   <  2:3659728/101‑1 (MQ=255)
                                          ggCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATc                                                                  >  1:1457696/1‑101 (MQ=255)
                                           gCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATcc                                                                 >  2:3153011/1‑101 (MQ=255)
                                             gTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCgg                                                               >  2:2396631/1‑101 (MQ=255)
                                              tGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTAtagatcggaag                                                              >  2:3091886/1‑91 (MQ=255)
                                                gATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCg                                                         >  1:3353572/1‑101 (MQ=255)
                                                 aTCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGa                                                        >  2:2725884/1‑101 (MQ=255)
                                                  tCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAg                                                       <  2:2415952/101‑1 (MQ=255)
                                                    gAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAgcg                                                     <  1:1603563/101‑1 (MQ=255)
                                                      aaCCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGcgcc                                                   <  2:2364187/101‑1 (MQ=255)
                                                         tccgatctgTGAGTTGCTCGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCAAGcgcctt                                                 <  2:2539471/93‑3 (MQ=25)
                                                         cTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCc.gcg                                               <  1:4102018/101‑1 (MQ=255)
                                                          tGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.gcg.c                                             <  2:1645035/101‑1 (MQ=255)
                                                           gcg.gcgTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.Ca                                            <  2:1406628/101‑1 (MQ=255)
                                                            cg.gcgTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.g                                          <  1:2204414/101‑1 (MQ=255)
                                                             g.gcgTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.Gt                                         >  2:927227/1‑101 (MQ=255)
                                                                ggTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCg                                       <  2:2811824/100‑1 (MQ=255)
                                                                cgTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCg                                       >  1:3001718/1‑101 (MQ=255)
                                                                cgTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCg                                       >  1:1466493/1‑101 (MQ=255)
                                                                 gTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGc                                      <  1:3251076/101‑1 (MQ=255)
                                                                  tGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CC.GTCGcc                                     >  2:863440/1‑100 (MQ=255)
                                                                  tGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGcc                                     >  2:2183684/1‑100 (MQ=255)
                                                                  tGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCt                                     <  1:1531343/101‑1 (MQ=255)
                                                                    aGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTcc                                   <  1:896238/101‑1 (MQ=255)
                                                                     gTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTccc                                  <  2:1457594/101‑1 (MQ=255)
                                                                     gTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTccc                                  <  2:3853157/101‑1 (MQ=255)
                                                                      ttGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTcccc                                 >  1:1105340/1‑101 (MQ=255)
                                                                      ttGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGATcccc                                 >  2:1357098/1‑101 (MQ=255)
                                                                       tGCCAGAGATAGA.CCCCGTAGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTccccc                                <  2:640224/101‑1 (MQ=255)
                                                                         ccAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCcg                              <  2:2588576/101‑1 (MQ=255)
                                                                          cAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCcgc                             <  1:1771026/101‑1 (MQ=255)
                                                                          cAGTGATAGA.CGCCGGGGA.CGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCcgc                             <  1:3174793/101‑1 (MQ=255)
                                                                           aGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCcgcg                            <  1:1937323/101‑1 (MQ=255)
                                                                              gATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCgggg                         >  1:2570368/1‑101 (MQ=255)
                                                                                 taggctccGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGc                       <  2:594455/95‑1 (MQ=255)
                                                                                   a.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTg                    >  1:2334848/1‑101 (MQ=255)
                                                                                   a.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTg                    <  2:1807291/101‑1 (MQ=255)
                                                                                       ccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGa                  <  2:4105530/92‑1 (MQ=9)
                                                                                       ccGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGAt                 <  1:736236/101‑1 (MQ=255)
                                                                                        caacctatgTCGCTCCCCCCGCGGGGGCGTGGATCGACACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGAt                 <  1:2949842/93‑1 (MQ=9)
                                                                                        caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGAt                 >  2:131135/9‑101 (MQ=9)
                                                                                        cGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATc                <  2:3001493/101‑1 (MQ=255)
                                                                                         gcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTgg                   <  2:3595977/95‑1 (MQ=2)
                                                                                         gTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGAC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCg               >  1:3631782/1‑101 (MQ=255)
                                                                                         aacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATc                >  1:2258866/8‑101 (MQ=9)
                                                                                          ccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATTC.A...TCGAGCGCCATCGAGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                 <  2:2962162/96‑1 (MQ=2)
                                                                                          acctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCg               <  2:125691/95‑1 (MQ=9)
                                                                                          acctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.CTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCg               <  1:2682860/95‑1 (MQ=9)
                                                                                           cctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGa              >  2:2696242/6‑101 (MQ=9)
                                                                                           cctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGa              >  2:169902/6‑101 (MQ=9)
                                                                                           ccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGAt                 <  1:1292728/97‑1 (MQ=2)
                                                                                           ccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGAt                 >  1:3729417/5‑101 (MQ=2)
                                                                                            gcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATTC.A...TCGAGCGCCATCGAGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg               <  1:3750520/98‑1 (MQ=2)
                                                                                            ctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa             >  2:1603855/5‑101 (MQ=9)
                                                                                            cggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATc                <  1:1021152/98‑1 (MQ=2)
                                                                                            cggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATc                >  2:1510153/4‑101 (MQ=2)
                                                                                              gcGTCGNTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa              <  1:647286/100‑1 (MQ=2)
                                                                                              atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc           <  1:566833/99‑1 (MQ=9)
                                                                                              atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc           <  1:3195485/99‑1 (MQ=9)
                                                                                                gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa            <  2:1988641/101‑1 (MQ=2)
                                                                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACGATg       >  2:442054/1‑101 (MQ=255)
                                                                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGc      <  2:368273/101‑1 (MQ=255)
                                                                                                     cccccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGt    <  1:1402217/100‑1 (MQ=255)
                                                                                                       cccccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcg  <  1:2536434/101‑4 (MQ=9)
                                                                                                       cccccGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACgcctcg     >  2:3956960/1‑96 (MQ=2)
                                                                                                       |                                                                                                            
ATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTC  >  NC_014034/1395739‑1395939

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG