Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,865 G→C 8.1% intergenic (+677/‑254) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,8650G→C 8.1% 37.5 ‑1.1 ‑1.0233intergenic (+677/‑254)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/12):  ref base (104/110):  total (111/122)
Fisher's exact test strand distribution p-value = 3.50e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.09e-01

Only 100 of 246 total aligned reads displayed.

TCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGG  >  NC_014034/1395765‑1395965
                                                                                                      |                                                                                                        
  gCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCTgcg                                                                                                        >  2:1239515/1‑101 (MQ=255)
  gCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGGGGAGGTCGCTCCCCCCCCGGGGGGGTGGATCGAAACGgcg                                                                                                        >  1:517506/1‑101 (MQ=37)
     ccccccGCGGGGGCGTGGATCGAAACAATGCGGCGCGA...GCCTATGGCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgcgtgcg                                                                                                    <  1:3571313/101‑5 (MQ=2)
     ccccccGCGGGGGCGTGGATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgccg                                                                                                     <  1:3422787/101‑1 (MQ=255)
     ccccccGCGGGGGCGTGGATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgccg                                                                                                     <  2:479080/101‑1 (MQ=255)
        cccGCGGGGGCGTGGATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCt                                                                                                  >  1:2128666/1‑101 (MQ=255)
        cccGCGGGGGCGTGGATCGAAAC.CTGCGGCGTCAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCt                                                                                                  <  2:1107604/101‑1 (MQ=255)
         ccGCGGGGACGTGGCTCGAAAC.CTGCGGCGTGATTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTa                                                                                                 <  1:3654887/101‑1 (MQ=255)
          cttccgatcttGGATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTAt                                                                                                <  1:3092116/91‑1 (MQ=255)
             gggggCGTGGATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGc                                                                                             >  1:1475076/1‑101 (MQ=255)
              ggggCGTGGATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGcc                                                                                            >  1:2700882/1‑100 (MQ=255)
               gggCGTGGATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCAt                                                                                           >  2:3288612/1‑101 (MQ=255)
               gggCGTGGATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCAt                                                                                           >  1:2542545/1‑101 (MQ=255)
                ggCGTGGATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATc                                                                                          >  1:1457696/1‑101 (MQ=255)
                ggCGTGGATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATc                                                                                          <  2:2549645/101‑1 (MQ=255)
                   gTGGATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCgg                                                                                       <  1:1643469/101‑1 (MQ=255)
                      gATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCg                                                                                 >  1:3353572/1‑101 (MQ=255)
                        tCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAg                                                                               <  2:2415952/101‑1 (MQ=255)
                         cGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgc                                                                              <  2:3056642/101‑1 (MQ=255)
                         cGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgc                                                                              >  1:2020339/1‑101 (MQ=255)
                          gAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgcg                                                                             >  2:1632337/1‑101 (MQ=255)
                           aaaC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgcgc                                                                            >  2:1770907/1‑101 (MQ=255)
                            aaC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGcgcc                                                                           >  2:403740/1‑101 (MQ=255)
                                  gcggcgTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCa                                                                      <  2:3110607/101‑1 (MQ=255)
                                   cggcgTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.g                                                                    <  1:2204414/101‑1 (MQ=255)
                                     gcgTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTc                                                                  <  2:3977821/101‑1 (MQ=255)
                                     gcgTGAGTTGCCAGTGATAGAC.GCCGTGGACGGCGCTCCCCCCGCGGGGGCGTGAATCGAAACTGCGCCGCCTTTCGCATCCGG...TCGAGCGCCGCGCA.GTc                                                                  <  2:3848802/101‑1 (MQ=255)
                                      ggTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCg                                                                 <  2:2811824/100‑1 (MQ=255)
                                       gTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGc                                                                <  1:3251076/101‑1 (MQ=255)
                                        tGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCC.GTCGcc                                                               >  2:863440/1‑100 (MQ=255)
                                        tGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGcc                                                               >  2:2183684/1‑100 (MQ=255)
                                         gAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTc                                                              <  2:2966121/101‑1 (MQ=255)
                                          aGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTcc                                                             <  1:896238/101‑1 (MQ=255)
                                            ttGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTcccc                                                           >  2:560565/1‑101 (MQ=255)
                                            ttGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGATcccc                                                           >  2:1357098/1‑101 (MQ=255)
                                            gtGCCAGTGATAGAC.GCCGTGGGCGTCGCTGCCCCCGCGGGCGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTcccc                                                           <  2:323376/100‑1 (MQ=255)
                                              gCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTcccccc                                                         <  2:631459/101‑1 (MQ=255)
                                                 aGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCcgcg                                                      <  1:1937323/101‑1 (MQ=255)
                                                  gTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCgg                                                     >  2:3587048/1‑101 (MQ=255)
                                                      tAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGc                                                 >  1:3736246/1‑101 (MQ=255)
                                                        gAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGt                                               <  2:928312/101‑1 (MQ=255)
                                                           gccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTgg                                             <  1:3194867/91‑1 (MQ=9)
                                                           gccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTgg                                             <  2:2361342/91‑1 (MQ=9)
                                                            gCCGTGGACGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGa                                            >  2:4177507/1‑101 (MQ=255)
                                                            ccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGa                                            <  2:4105530/92‑1 (MQ=9)
                                                             ggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTg                                              <  2:2892716/94‑1 (MQ=2)
                                                             caacctatgTCGCTCCCCCCGCGGGGGCGTGGATCGACACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGAt                                           <  1:2949842/93‑1 (MQ=9)
                                                             caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGAt                                           <  1:1743566/93‑1 (MQ=9)
                                                             caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGAt                                           >  2:131135/9‑101 (MQ=9)
                                                              cGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATc                                          <  2:3001493/101‑1 (MQ=255)
                                                              aacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATc                                          <  2:1961005/94‑1 (MQ=9)
                                                               gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                         >  2:2367051/1‑101 (MQ=255)
                                                               gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                         >  1:2813890/1‑101 (MQ=255)
                                                               cccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGa                                            >  1:227575/6‑101 (MQ=0)
                                                               acctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCg                                         <  2:125691/95‑1 (MQ=9)
                                                                cctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                        >  2:2696242/6‑101 (MQ=9)
                                                                cctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                        >  2:169902/6‑101 (MQ=9)
                                                                ccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                           <  1:1292728/97‑1 (MQ=2)
                                                                 ctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                       >  2:1603855/5‑101 (MQ=9)
                                                                 ctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                       <  1:118355/97‑1 (MQ=9)
                                                                 ctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                       <  1:3883869/97‑1 (MQ=9)
                                                                 cggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                          >  2:1510153/4‑101 (MQ=2)
                                                                 cggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                          <  1:1021152/98‑1 (MQ=2)
                                                                  ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                         >  1:695441/3‑101 (MQ=2)
                                                                  aaCGTCTCTCCGCCCGCGTGGTCGTGACTCGAAACTGCGCCACCTATCGCATACGA...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGTCGTGGATCGaaa                                      <  2:1398516/100‑1 (MQ=25)
                                                                   gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                        <  2:463520/100‑1 (MQ=2)
                                                                   atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                     <  1:566833/99‑1 (MQ=9)
                                                                       cgcgcaccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCGCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATg                                 <  2:2102575/95‑1 (MQ=9)
                                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGc                                <  2:368273/101‑1 (MQ=255)
                                                                          tcaccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                              >  2:1811533/4‑100 (MQ=9)
                                                                           caccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggc                             >  1:574941/3‑99 (MQ=9)
                                                                            cccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTc                            >  1:1570484/1‑101 (MQ=255)
                                                                            cccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTc                            <  2:1437400/101‑1 (MQ=255)
                                                                            accccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcg                            >  2:1182766/2‑98 (MQ=9)
                                                                             ccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgc                           <  1:1906429/101‑5 (MQ=9)
                                                                             ccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACAATGCggcgc                           >  2:919115/1‑97 (MQ=9)
                                                                               ccGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccccgaac                            <  1:143529/101‑10 (MQ=2)
                                                                                cgcgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGCATCGAAACGATGCGTTCATgg                        <  1:3122584/101‑1 (MQ=255)
                                                                                  cgGGGGGGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTCCATCGcg                      <  2:960492/101‑2 (MQ=255)
                                                                                  cgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGAC.G...CCGGGGCCCTCGCACGTCGCTCCCCCCGAGGGGGCGTGGATCGAAACAATGCggcgcgggcc                      <  1:4155113/101‑10 (MQ=2)
                                                                                  cgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                      <  2:3213144/101‑1 (MQ=255)
                                                                                  cgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                      >  2:669543/1‑101 (MQ=255)
                                                                                  cgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCCGGGGCGTGGATCGACACGCTGCGGTGATGGcc                      >  2:3604704/1‑101 (MQ=38)
                                                                                   gggggCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcct                     <  1:243624/101‑11 (MQ=2)
                                                                                   gggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                     >  2:2713037/1‑101 (MQ=255)
                                                                                    ggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTa                    <  2:3735992/101‑1 (MQ=255)
                                                                                      ggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                  <  2:3899934/101‑1 (MQ=255)
                                                                                      ggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GGCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                  <  1:1357198/101‑1 (MQ=255)
                                                                                          tGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGCAACGATGCGTTCATGGCCTACGTccg              >  2:1826148/1‑99 (MQ=255)
                                                                                          tGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTCCATGGCCTACGTCgg              >  1:728862/1‑101 (MQ=255)
                                                                                           ggATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCggg             >  1:3526896/1‑101 (MQ=255)
                                                                                               cGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt         <  2:3468658/101‑1 (MQ=255)
                                                                                               cGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt         <  1:1239611/101‑1 (MQ=255)
                                                                                               cGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGACTACGTCGGGCAGt         >  1:4206819/1‑101 (MQ=255)
                                                                                                gAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTg        <  2:1474974/101‑1 (MQ=255)
                                                                                                  aaCTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGa      <  1:2453567/101‑1 (MQ=255)
                                                                                                   aCTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGAc     >  1:4065437/1‑101 (MQ=255)
                                                                                                    cTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACt    <  1:2396807/101‑1 (MQ=255)
                                                                                                     tGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTg   <  1:2615145/101‑1 (MQ=255)
                                                                                                      gcgcCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTgg  <  2:2739605/101‑1 (MQ=255)
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TCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CTGCGGCGTGAGTTGCCAGTGATAGAC.GCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGG  >  NC_014034/1395765‑1395965

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG