Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,887 G→T 7.9% intergenic (+699/‑232) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,8870G→T 7.9% 38.4 ‑1.1 ‑0.3228intergenic (+699/‑232)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/12):  ref base (95/113):  total (102/126)
Fisher's exact test strand distribution p-value = 3.37e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.49e-01

Only 100 of 238 total aligned reads displayed.

GATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTG  >  NC_014034/1395787‑1395987
                                                                                                       |                                                                                                     
    gAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgcg                                                                                                   <  1:1603563/101‑1 (MQ=255)
     aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgcgc                                                                                                  >  1:1354526/1‑101 (MQ=255)
       aCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGcgccg                                                                                                <  2:2612379/101‑1 (MQ=255)
        tccgatctgTGAGTTGCTCGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCAAGcgcctt                                                                                               <  2:2539471/93‑3 (MQ=25)
          tGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCgcgc                                                                                             <  2:1645035/101‑1 (MQ=255)
            cggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.g                                                                                          <  1:536646/101‑1 (MQ=255)
            cggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.g                                                                                          <  1:2204414/101‑1 (MQ=255)
            cggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.g                                                                                          <  1:1925214/101‑1 (MQ=255)
              gcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTc                                                                                        <  2:3977821/101‑1 (MQ=255)
               ggTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCg                                                                                       <  2:2811824/100‑1 (MQ=255)
               cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCg                                                                                       >  1:3001718/1‑101 (MQ=255)
                gTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGc                                                                                      <  1:3251076/101‑1 (MQ=255)
                gTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCAAGcgccttagatcgga                                                                                       >  1:2539662/1‑91 (MQ=37)
                 tGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGcc                                                                                     >  2:2183684/1‑100 (MQ=255)
                  gAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTc                                                                                    <  2:2966121/101‑1 (MQ=255)
                   aGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTcc                                                                                   <  1:896238/101‑1 (MQ=255)
                   aGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTcc                                                                                   <  2:3013577/101‑1 (MQ=255)
                    gTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTccc                                                                                  <  2:3853157/101‑1 (MQ=255)
                       gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTcccccc                                                                               <  2:631459/101‑1 (MQ=255)
                        ccAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCcg                                                                              <  2:1802237/101‑1 (MQ=255)
                         cAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCcgc                                                                             <  1:1771026/101‑1 (MQ=255)
                         cAGTGATAGACGCCGGGGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCcgc                                                                             <  1:3174793/101‑1 (MQ=255)
                             gATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCgggg                                                                         >  1:2727431/1‑101 (MQ=255)
                              aTAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GACGCTCCCCCCGCggggg                                                                        >  2:3801410/1‑101 (MQ=255)
                               tAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGc                                                                       >  2:2841934/1‑101 (MQ=255)
                               tAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGc                                                                       >  1:3736246/1‑101 (MQ=255)
                               tAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGc                                                                       >  2:907223/1‑101 (MQ=255)
                                 gACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGt                                                                     <  2:928312/101‑1 (MQ=255)
                                    gCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                  >  2:1354104/1‑101 (MQ=255)
                                      gcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTgg                                                                   <  2:3595977/95‑1 (MQ=2)
                                      aacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                >  2:1912464/8‑101 (MQ=9)
                                       cccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                  >  1:227575/6‑101 (MQ=0)
                                         ctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                             <  1:3883869/97‑1 (MQ=9)
                                         ctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                             <  1:118355/97‑1 (MQ=9)
                                         cggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                <  1:1021152/98‑1 (MQ=2)
                                          tatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                            >  2:1396851/4‑101 (MQ=9)
                                          tatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                            <  1:2696448/98‑1 (MQ=9)
                                          ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                               >  1:695441/3‑101 (MQ=2)
                                          gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                            <  2:1457743/101‑1 (MQ=255)
                                           gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                              >  2:3674657/2‑101 (MQ=2)
                                            cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                             <  2:1262196/101‑1 (MQ=2)
                                            cGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                                          <  2:1496168/101‑1 (MQ=255)
                                             gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                            <  2:1988641/101‑1 (MQ=2)
                                              tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                        >  2:3726045/1‑101 (MQ=255)
                                               cgcgcaccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCGCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATg                                                       <  2:2102575/95‑1 (MQ=9)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACGATg                                                       >  2:442054/1‑101 (MQ=255)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATgg                                                      >  2:837254/1‑100 (MQ=255)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGc                                                      >  1:4118582/1‑101 (MQ=255)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGc                                                      >  2:2726735/1‑101 (MQ=255)
                                                   ccccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGtt                                                   <  2:3517979/101‑1 (MQ=255)
                                                   caccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggc                                                   >  1:574941/3‑99 (MQ=9)
                                                    cccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTc                                                  <  2:1437400/101‑1 (MQ=255)
                                                    cccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTc                                                  >  1:1570484/1‑101 (MQ=255)
                                                    cccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTc                                                  <  1:3342516/101‑1 (MQ=255)
                                                    accccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcg                                                  >  2:1182766/2‑98 (MQ=9)
                                                       ccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATg                                               <  2:1820651/101‑1 (MQ=255)
                                                       ccGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccccgaac                                                  <  1:143529/101‑10 (MQ=2)
                                                       ccGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccccgaac                                                  <  1:2855119/101‑10 (MQ=2)
                                                        cgcgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATgg                                              <  2:170277/101‑1 (MQ=255)
                                                        cgcgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGCATCGAAACGATGCGTTCATgg                                              <  1:3122584/101‑1 (MQ=255)
                                                         gcgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggc                                             <  1:1396950/101‑9 (MQ=2)
                                                          cgGGGGGGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTCCATCGcg                                            <  2:960492/101‑2 (MQ=255)
                                                          cgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                                            >  1:356700/1‑101 (MQ=255)
                                                           gggggCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcct                                           <  1:243624/101‑11 (MQ=2)
                                                           gggggCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcct                                           >  2:1630939/1‑91 (MQ=2)
                                                           gggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                           <  2:3278775/101‑1 (MQ=255)
                                                           gggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                           >  2:2713037/1‑101 (MQ=255)
                                                            ggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTa                                          <  2:1570375/101‑1 (MQ=255)
                                                            ggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTa                                          <  1:3587301/101‑1 (MQ=255)
                                                             gggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                                         >  1:2032323/1‑101 (MQ=255)
                                                             gggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                                         >  2:477644/1‑101 (MQ=255)
                                                              ggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                                        <  2:3215140/101‑1 (MQ=255)
                                                              ggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                                        <  2:3190859/101‑1 (MQ=255)
                                                                 gTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCg                                     <  2:3000803/101‑1 (MQ=255)
                                                                       cGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                               <  2:3468658/101‑1 (MQ=255)
                                                                       cGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                               >  1:3821589/1‑101 (MQ=255)
                                                                       cGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                               <  1:1239611/101‑1 (MQ=255)
                                                                       cGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGACTACGTCGGGCAGt                               >  1:4206819/1‑101 (MQ=255)
                                                                        gAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTg                              <  2:2396833/101‑1 (MQ=255)
                                                                         aaaCTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTgg                             <  2:56106/101‑1 (MQ=255)
                                                                          aaCTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGa                            <  1:2453567/101‑1 (MQ=255)
                                                                           aCTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGAc                           >  2:4173931/1‑101 (MQ=255)
                                                                           aCTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGAc                           >  1:4065437/1‑101 (MQ=255)
                                                                             tGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTg                         <  2:1239899/101‑1 (MQ=255)
                                                                                gccgccTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTc                      <  1:4177769/101‑1 (MQ=255)
                                                                                  cgccTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGCCGGGCCGTGGACTGGTCGc                    >  2:2718910/1‑101 (MQ=255)
                                                                                      tATCGCATCCGG...TCGAGCGCCGGGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc                <  1:1826268/101‑1 (MQ=255)
                                                                                      tATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTctc                >  2:1869003/1‑99 (MQ=255)
                                                                                      tATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc                >  2:69409/1‑101 (MQ=255)
                                                                                       aTCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccc               >  1:2119717/1‑101 (MQ=255)
                                                                                       aTCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccc               <  2:4081659/101‑1 (MQ=255)
                                                                                         cGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccccc             <  1:806311/101‑1 (MQ=255)
                                                                                          gCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcg            <  1:2340576/101‑1 (MQ=255)
                                                                                            aTCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcgcg          >  1:648428/1‑101 (MQ=255)
                                                                                            aTCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcgcg          >  2:236290/1‑101 (MQ=255)
                                                                                                gg...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggggg      <  1:3517226/101‑1 (MQ=255)
                                                                                                 g...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGc     >  2:3925998/1‑101 (MQ=255)
                                                                                                     tCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCg    <  1:2627561/101‑1 (MQ=255)
                                                                                                       gAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTg  >  1:2812189/1‑101 (MQ=255)
                                                                                                       gAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTg  <  2:2335735/101‑1 (MQ=255)
                                                                                                       |                                                                                                     
GATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTG  >  NC_014034/1395787‑1395987

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG