Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,890 C→T 8.1% intergenic (+702/‑229) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,8900C→T 8.1% 36.7 ‑1.1 ‑0.0221intergenic (+702/‑229)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/12):  ref base (94/108):  total (100/121)
Fisher's exact test strand distribution p-value = 3.30e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.57e-01

Only 100 of 243 total aligned reads displayed.

CGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGAT  >  NC_014034/1395790‑1395990
                                                                                                       |                                                                                                     
cGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgc                                                                                                       <  2:3056642/101‑1 (MQ=255)
 gAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgcg                                                                                                      >  2:1632337/1‑101 (MQ=255)
  aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgcgc                                                                                                     >  1:1354526/1‑101 (MQ=255)
     tccgatctgTGAGTTGCTCGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCAAGcgcctt                                                                                                  <  2:2539471/93‑3 (MQ=25)
      cTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCcgcg                                                                                                 <  1:4102018/101‑1 (MQ=255)
           gcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTc                                                                                           <  2:3977821/101‑1 (MQ=255)
            ggTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCg                                                                                          <  2:2811824/100‑1 (MQ=255)
            cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCg                                                                                          >  1:1466493/1‑101 (MQ=255)
              tGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGcc                                                                                        >  2:2183684/1‑100 (MQ=255)
               gAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTc                                                                                       <  2:2966121/101‑1 (MQ=255)
                aGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTcc                                                                                      <  1:896238/101‑1 (MQ=255)
                 gTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTccc                                                                                     <  2:3853157/101‑1 (MQ=255)
                  ttGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTcccc                                                                                    >  2:560565/1‑101 (MQ=255)
                  gtGCCAGTGATAGACGCCGTGGGCGTCGCTGCCCCCGCGGGCGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTcccc                                                                                    <  2:323376/100‑1 (MQ=255)
                    gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTcccccc                                                                                  <  2:631459/101‑1 (MQ=255)
                     ccAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCcg                                                                                 <  2:1802237/101‑1 (MQ=255)
                      cAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCcgc                                                                                <  1:1771026/101‑1 (MQ=255)
                       aGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCcgcg                                                                               <  1:1937323/101‑1 (MQ=255)
                          gATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCgggg                                                                            >  1:2570368/1‑101 (MQ=255)
                            tAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGc                                                                          >  2:907223/1‑101 (MQ=255)
                            tAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGc                                                                          >  1:3736246/1‑101 (MQ=255)
                              gACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGt                                                                        <  2:928312/101‑1 (MQ=255)
                               aCGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTg                                                                       >  1:2334848/1‑101 (MQ=255)
                                gccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTgg                                                                      <  2:2361342/91‑1 (MQ=9)
                                cGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTgg                                                                      >  1:3279016/1‑101 (MQ=255)
                                 gCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                     >  2:1354104/1‑101 (MQ=255)
                                 ccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGa                                                                     <  2:4105530/92‑1 (MQ=9)
                                  ggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTg                                                                       <  2:2892716/94‑1 (MQ=2)
                                  caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                    >  2:131135/9‑101 (MQ=9)
                                   cGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   <  2:3001493/101‑1 (MQ=255)
                                   aacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   >  2:1912464/8‑101 (MQ=9)
                                    gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                  >  1:2813890/1‑101 (MQ=255)
                                    gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                  >  2:2367051/1‑101 (MQ=255)
                                    acctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGCTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                  <  1:2682860/95‑1 (MQ=9)
                                     cctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                 >  2:2696242/6‑101 (MQ=9)
                                      ctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                <  1:3883869/97‑1 (MQ=9)
                                      ctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                <  1:118355/97‑1 (MQ=9)
                                      cggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   >  2:1510153/4‑101 (MQ=2)
                                      cggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   <  1:1021152/98‑1 (MQ=2)
                                       gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                               <  2:1457743/101‑1 (MQ=255)
                                       aaCGTCTCTCCGCCCGCGTGGTCGTGACTCGAAACTGCGCCACCTATCGCATACGA...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGTCGTGGATCGaaa                                                               <  2:1398516/100‑1 (MQ=25)
                                        gcGTCGNTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                 <  1:647286/100‑1 (MQ=2)
                                        atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                              <  1:3195485/99‑1 (MQ=9)
                                        aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                              >  2:1671594/1‑101 (MQ=255)
                                          gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                               <  2:1988641/101‑1 (MQ=2)
                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACGATg                                                          >  2:442054/1‑101 (MQ=255)
                                             gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATgg                                                         >  2:837254/1‑100 (MQ=255)
                                             gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGc                                                         <  2:368273/101‑1 (MQ=255)
                                               tcaccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                                                       >  2:2295117/4‑100 (MQ=9)
                                               tcaccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                                                       >  2:1811533/4‑100 (MQ=9)
                                                 cccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcg                                                     <  1:2536434/101‑4 (MQ=9)
                                                 cccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTc                                                     <  2:1437400/101‑1 (MQ=255)
                                                 cccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTc                                                     >  1:1570484/1‑101 (MQ=255)
                                                 accccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcg                                                     >  2:1182766/2‑98 (MQ=9)
                                                  ccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                                    <  1:554291/101‑1 (MQ=255)
                                                   cccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcg                                                   >  1:1713135/1‑96 (MQ=9)
                                                      gcgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggc                                                <  1:1396950/101‑9 (MQ=2)
                                                      gcgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggc                                                >  2:3571060/1‑93 (MQ=2)
                                                       cgGGGGGGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTCCATCGcg                                               <  2:960492/101‑2 (MQ=255)
                                                       cgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcc                                               >  1:2140057/1‑92 (MQ=2)
                                                       cgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                                               <  2:3213144/101‑1 (MQ=255)
                                                       cgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                                               >  2:669543/1‑101 (MQ=255)
                                                       cgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                                               >  1:356700/1‑101 (MQ=255)
                                                       cgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCCGGGGCGTGGATCGACACGCTGCGGTGATGGcc                                               >  2:3604704/1‑101 (MQ=38)
                                                        gggggCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcct                                              <  1:243624/101‑11 (MQ=2)
                                                        gggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                              <  2:3278775/101‑1 (MQ=255)
                                                        gggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                              >  2:2713037/1‑101 (MQ=255)
                                                         ggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTa                                             <  1:3587301/101‑1 (MQ=255)
                                                         ggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTa                                             <  2:1570375/101‑1 (MQ=255)
                                                          gggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                                            >  1:2032323/1‑101 (MQ=255)
                                                          gggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                                            >  2:477644/1‑101 (MQ=255)
                                                               tGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTCCATGGCCTACGTCgg                                       >  1:728862/1‑101 (MQ=255)
                                                                  aTCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCa                                    >  1:2235464/1‑101 (MQ=255)
                                                                  aTCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCa                                    >  2:3757812/1‑101 (MQ=255)
                                                                    cGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                                  <  1:1239611/101‑1 (MQ=255)
                                                                       aaCTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGa                               <  1:2927946/101‑1 (MQ=255)
                                                                        aCTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGAc                              >  2:4173931/1‑101 (MQ=255)
                                                                         cTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACt                             <  1:2396807/101‑1 (MQ=255)
                                                                          tGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTg                            <  2:1239899/101‑1 (MQ=255)
                                                                          tGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTg                            >  1:3112920/1‑101 (MQ=255)
                                                                          tGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTg                            >  1:3160985/1‑101 (MQ=255)
                                                                           gcgcCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTgg                           <  2:2739605/101‑1 (MQ=255)
                                                                             gccgccTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTc                         <  1:4177769/101‑1 (MQ=255)
                                                                             gccgccTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTc                         <  1:39108/101‑1 (MQ=255)
                                                                              ccgccTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCg                        >  1:2763750/1‑101 (MQ=255)
                                                                               cgccTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGc                       <  1:2726941/101‑1 (MQ=255)
                                                                                  cTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcc                    >  1:4121704/1‑101 (MQ=255)
                                                                                   tATCGCATCCGG...TCGAGCGCCGGGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc                   <  1:1826268/101‑1 (MQ=255)
                                                                                   tATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTctc                   >  2:1869003/1‑99 (MQ=255)
                                                                                      cGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccccc                <  1:806311/101‑1 (MQ=255)
                                                                                       gCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcg               <  1:493445/101‑1 (MQ=255)
                                                                                            cGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTTGTCGCTCCCCCCGCggcg          >  2:3506304/1‑99 (MQ=255)
                                                                                             gg...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggggg         <  1:3517226/101‑1 (MQ=255)
                                                                                              g...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGc        >  2:3925998/1‑101 (MQ=255)
                                                                                                     aGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTgg    >  2:1718650/1‑101 (MQ=255)
                                                                                                     aGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTgg    <  2:2032185/101‑1 (MQ=255)
                                                                                                     aGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTgg    >  1:660420/1‑101 (MQ=255)
                                                                                                       cgccgcGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGAt  <  1:3767312/101‑1 (MQ=255)
                                                                                                       cgccgcGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGAt  <  2:2725526/101‑1 (MQ=255)
                                                                                                       cgccgcGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGAt  <  2:3835334/101‑1 (MQ=255)
                                                                                                       |                                                                                                     
CGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGAT  >  NC_014034/1395790‑1395990

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG