Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,961 A→T 2.1% intergenic (+773/‑158) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,9610A→T 2.1% 3.0 ‑3.0 ‑0.4194intergenic (+773/‑158)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (0/4):  ref base (101/89):  total (101/93)
Fisher's exact test strand distribution p-value = 5.10e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.94e-01

Only 100 of 203 total aligned reads displayed.

AACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGA  >  NC_014034/1395861‑1396060
                                                                                                    |                                                                                                   
aaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGa                                                                                                     <  1:2927946/101‑1 (MQ=255)
aaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGa                                                                                                     <  1:2453567/101‑1 (MQ=255)
  cTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACt                                                                                                   >  2:3273359/1‑101 (MQ=255)
  cTGCGCCGCCTATCGCACCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACt                                                                                                   <  2:1129390/101‑1 (MQ=255)
   tGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTg                                                                                                  >  1:3160985/1‑101 (MQ=255)
    gcgcCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTgg                                                                                                 <  2:2739605/101‑1 (MQ=255)
      gccgccTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTc                                                                                               <  1:4177769/101‑1 (MQ=255)
       ccgccTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCg                                                                                              >  1:1325941/1‑101 (MQ=255)
       ccgccTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCg                                                                                              >  1:2763750/1‑101 (MQ=255)
        cgccTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGc                                                                                             <  1:2726941/101‑1 (MQ=255)
        cgccTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGCCGGGCCGTGGACTGGTCGc                                                                                             >  2:2718910/1‑101 (MQ=255)
           cTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcc                                                                                          >  1:4121704/1‑101 (MQ=255)
            tATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTctc                                                                                         >  2:1869003/1‑99 (MQ=255)
             aTCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccc                                                                                        >  1:2119717/1‑101 (MQ=255)
             aTCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGGCGCTcccc                                                                                        >  2:1941016/1‑101 (MQ=255)
                gCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcg                                                                                     <  1:2340576/101‑1 (MQ=255)
                     cGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTTGTCGCTCCCCCCGCggcg                                                                                >  2:3506304/1‑99 (MQ=255)
                       gTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGc                                                                              <  2:2000193/101‑1 (MQ=255)
                        tCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCg                                                                             <  1:2627561/101‑1 (MQ=255)
                          gAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTg                                                                           <  2:2335735/101‑1 (MQ=255)
                           aGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTgg                                                                          <  2:2032185/101‑1 (MQ=255)
                           aGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTgg                                                                          >  1:660420/1‑101 (MQ=255)
                             cgccgcGCAGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                        <  2:4206557/101‑1 (MQ=255)
                               ccgcGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      <  1:951165/101‑1 (MQ=255)
                               ccgcGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      <  1:560629/101‑1 (MQ=255)
                               acaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGCTCGACCGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      <  1:2829410/94‑1 (MQ=2)
                                cgcgCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                     <  1:647358/101‑1 (MQ=255)
                                cgcgCAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                     <  1:3506557/101‑1 (MQ=255)
                                 aggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGCTCGACCGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    <  1:2757997/96‑1 (MQ=2)
                                   gcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGGCGCTCCCCCCGCGGGGGCGGGGTTCGAAAc                                                                  >  2:1654527/1‑101 (MQ=255)
                                    cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                                 <  1:1219046/101‑1 (MQ=255)
                                    cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                                 <  1:429437/101‑1 (MQ=255)
                                    cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                                 <  2:2570177/101‑1 (MQ=255)
                                     aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTc                                                                >  1:453445/1‑101 (MQ=255)
                                      gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCg                                                               >  1:2027708/1‑101 (MQ=255)
                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGcc                                                             <  2:4065176/101‑1 (MQ=255)
                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGCTCGACCGAGTTCACGGCGGGCTCTCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAAcggggcg                                                            >  2:4229110/1‑95 (MQ=2)
                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGccc                                                            <  2:1717365/101‑1 (MQ=255)
                                           tCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTc                                                          <  2:1787259/101‑1 (MQ=255)
                                           tCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTc                                                          >  1:1561215/1‑101 (MQ=255)
                                            ccccccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCg                                                         >  2:1264948/1‑101 (MQ=255)
                                              ccccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGAt                                                       <  2:4078649/101‑1 (MQ=255)
                                               tctgcgGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATc                                                      <  1:3282445/98‑1 (MQ=255)
                                                ccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATcc                                                     >  2:1558861/1‑101 (MQ=255)
                                                ccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATcc                                                     >  2:2858325/1‑101 (MQ=255)
                                                ccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATcc                                                     <  2:893048/101‑1 (MQ=255)
                                                ccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATcc                                                     <  2:728786/101‑1 (MQ=255)
                                                ccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATcc                                                     <  2:587756/101‑1 (MQ=255)
                                                ccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATcc                                                     <  1:2193746/101‑1 (MQ=255)
                                                ccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATcc                                                     <  1:479120/101‑1 (MQ=255)
                                                     ggggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCt                                                >  1:2776784/1‑101 (MQ=255)
                                                      gggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCtt                                               <  1:837335/101‑1 (MQ=255)
                                                       ggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTg                                              <  1:3801667/101‑1 (MQ=255)
                                                       ggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTg                                              >  1:3331826/1‑101 (MQ=255)
                                                            ggATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCa                                         <  2:2516042/101‑1 (MQ=255)
                                                            ggATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCa                                         <  1:4062206/101‑1 (MQ=255)
                                                             gATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCAc                                        >  2:279452/1‑101 (MQ=255)
                                                             gATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCAc                                        >  2:1899573/1‑101 (MQ=255)
                                                              aTCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACg                                       >  2:2952092/1‑101 (MQ=255)
                                                              aTCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCCCGGGGCGGTGGTTCGAAACTGCCCCCCAATCCCCCCTTCCCTCACg                                       >  2:1020354/1‑101 (MQ=255)
                                                               tCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGc                                      <  1:236328/101‑1 (MQ=255)
                                                               tCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGc                                      <  2:312702/101‑1 (MQ=255)
                                                                 gAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCTGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACCCCt                                    >  1:3901147/1‑101 (MQ=255)
                                                                 gAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCt                                    >  1:801909/1‑101 (MQ=255)
                                                                  aaaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCGCCAATCCGCGCTTGTCTCAAGcccc                                   >  2:1503886/1‑99 (MQ=37)
                                                                  aaaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTg                                   <  2:4118321/101‑1 (MQ=255)
                                                                  aaaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTg                                   >  2:2935783/1‑101 (MQ=255)
                                                                   aaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGc                                  >  1:3849187/1‑101 (MQ=255)
                                                                    aCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGcc                                 >  1:3076346/1‑101 (MQ=255)
                                                                    aCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGcc                                 <  2:3353327/101‑1 (MQ=255)
                                                                    aCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGcc                                 >  2:380639/1‑101 (MQ=255)
                                                                      gATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGAGCTTGCCTCACGCCTGCcgc                               >  2:207810/1‑101 (MQ=255)
                                                                       aTGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCcgcg                              >  2:2241609/1‑101 (MQ=255)
                                                                        tGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGt                             <  2:1325841/101‑1 (MQ=255)
                                                                           gTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGc                          >  2:49791/1‑101 (MQ=255)
                                                                            ttCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCt                         >  1:1893527/1‑101 (MQ=255)
                                                                             tCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTc                        >  1:1669369/1‑101 (MQ=255)
                                                                             tCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTc                        >  1:3974919/1‑101 (MQ=255)
                                                                              cATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTcc                       >  2:381471/1‑101 (MQ=255)
                                                                               aTGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTccc                      >  1:1948476/1‑101 (MQ=255)
                                                                                   ccTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATAGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCcg                  >  2:3662796/1‑101 (MQ=255)
                                                                                   ccTACGTC.GGCAGTGGACTGGTCCCTCCCCCCGCGGGGGCGGGGATCGAAACCCGCCCCCGATCCCCGCTTGCCCTCCCCCCGCCGCGTCCCTcccccccc                 >  2:779704/1‑99 (MQ=19)
                                                                                     tACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCcgcg                >  2:3922200/1‑101 (MQ=255)
                                                                                      aCGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCgg               >  1:1394423/1‑101 (MQ=255)
                                                                                      aCGGCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCgg               <  1:1141378/101‑1 (MQ=255)
                                                                                       cGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGGTCCCCCCGCggg              >  2:3714462/1‑101 (MQ=255)
                                                                                       cGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCggg              >  1:2530835/1‑101 (MQ=255)
                                                                                        gTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCgggg             <  2:2390722/101‑1 (MQ=255)
                                                                                        gTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCgggg             >  1:3532398/1‑101 (MQ=255)
                                                                                        gTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCCAAACTCGCCCTCGATCCGCGCTGGCCTCACGCCTGCCGCGTCGCTCCCCCCGCgggg             >  1:3617631/1‑101 (MQ=255)
                                                                                          cGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCGGGGGc           >  2:2140196/1‑101 (MQ=255)
                                                                                          cGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCGGGGGc           <  1:3592476/101‑1 (MQ=255)
                                                                                             gCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCGGGGGCGTg        <  1:1799531/101‑1 (MQ=255)
                                                                                              cAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCTTCGCTCCCCCCGCGGGGGCGTgg       <  2:453388/101‑1 (MQ=255)
                                                                                                 tGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCGGGGGCGTGGATc    <  1:927312/101‑1 (MQ=255)
                                                                                                 tGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGAGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCGGGGGCGTGGATc    <  1:207848/101‑1 (MQ=255)
                                                                                                  ggACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCGGGGGCGTGGATCg   <  1:1699904/101‑1 (MQ=255)
                                                                                                  ggACTGGTCGCCCCCCCCGCGGGGGCGGGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCGGGGGCGTGGATCg   <  1:755895/101‑1 (MQ=255)
                                                                                                   gACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa  <  1:2842153/101‑1 (MQ=255)
                                                                                                   gACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa  >  2:668061/1‑101 (MQ=255)
                                                                                                    |                                                                                                   
AACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGA  >  NC_014034/1395861‑1396060

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG