Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,000 C→G 17.2% intergenic (+812/‑119) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,0000C→G17.2% 101.0 ‑0.9 ‑0.0238intergenic (+812/‑119)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (19/22):  ref base (106/91):  total (125/113)
Fisher's exact test strand distribution p-value = 3.96e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.57e-01

Only 100 of 261 total aligned reads displayed.

CGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGT  >  NC_014034/1395901‑1396100
                                                                                                   |                                                                                                        
 gCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGccc                                                                                                        <  1:2726090/101‑1 (MQ=255)
  cTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCt                                                                                                       <  1:152801/101‑1 (MQ=255)
   tCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTc                                                                                                      >  1:1561215/1‑101 (MQ=255)
      ccccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGAt                                                                                                   <  2:4078649/101‑1 (MQ=255)
       tctgcgGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATc                                                                                                  <  1:3282445/98‑1 (MQ=255)
        ccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATcc                                                                                                 >  2:2858325/1‑101 (MQ=255)
        ccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATcc                                                                                                 >  2:1558861/1‑101 (MQ=255)
        ccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATcc                                                                                                 <  2:587756/101‑1 (MQ=255)
        ccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATcc                                                                                                 <  1:2193746/101‑1 (MQ=255)
          gcgGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATcaga                                                                                               >  2:3282204/1‑98 (MQ=255)
             ggggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCt                                                                                            >  1:928186/1‑101 (MQ=255)
               ggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTg                                                                                          >  1:3331826/1‑101 (MQ=255)
                  gTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCt                                                                                       <  1:3758069/101‑1 (MQ=255)
                    ggATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCa                                                                                     <  1:403791/101‑1 (MQ=255)
                     gATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCAc                                                                                    <  2:2761654/101‑1 (MQ=255)
                      aTCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCCCGGGGCGGTGGTTCGAAACTGCCCCCCAATCCCCCCTTCCCTCACg                                                                                   >  2:1020354/1‑101 (MQ=255)
                        cGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGcc                                                                                 >  2:458424/1‑101 (MQ=255)
                         gAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCt                                                                                >  2:3810823/1‑101 (MQ=255)
                         gAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATAGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCt                                                                                >  1:3505229/1‑101 (MQ=255)
                           aaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGc                                                                              >  1:3849187/1‑101 (MQ=255)
                           aaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGc                                                                              >  2:867128/1‑101 (MQ=255)
                            aCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGc.c                                                                            >  1:234124/1‑101 (MQ=255)
                              gATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.cgc                                                                          <  2:928101/101‑1 (MQ=255)
                                    ttCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCt                                                                 >  2:542412/1‑101 (MQ=255)
                                     tCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTc                                                                <  1:907308/101‑1 (MQ=255)
                                     tCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTc                                                                >  1:3974919/1‑101 (MQ=255)
                                       aTGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTccc                                                              >  1:1674952/1‑101 (MQ=255)
                                       aTGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTccc                                                              >  1:1948476/1‑101 (MQ=255)
                                         ggCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTccccc                                                            >  2:2889676/1‑101 (MQ=255)
                                           ccTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATAGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCcg                                                          >  2:3662796/1‑101 (MQ=255)
                                            cTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCcgc                                                         >  2:1898425/1‑101 (MQ=255)
                                             tACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCcgcg                                                        >  2:755818/1‑101 (MQ=255)
                                              aCGGCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCgg                                                       <  1:1141378/101‑1 (MQ=255)
                                               cGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCggg                                                      >  1:2530835/1‑101 (MQ=255)
                                                gTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCgggg                                                     <  2:2390722/101‑1 (MQ=255)
                                                  cGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGc                                                   <  1:3592476/101‑1 (MQ=255)
                                                   gggCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCg                                                  >  1:1373115/1‑101 (MQ=255)
                                                    ggCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCgg                                                 >  1:1708604/1‑100 (MQ=255)
                                                     gCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTg                                                <  1:1799531/101‑1 (MQ=255)
                                                      cAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..T.TCGCTCCCCCCGCGGGGGCGTgg                                               <  2:453388/101‑1 (MQ=255)
                                                      cAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTgg                                               <  1:458481/101‑1 (MQ=255)
                                                      cAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTgg                                               >  1:2093247/1‑101 (MQ=255)
                                                        gTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGAt                                             <  1:77862/101‑1 (MQ=255)
                                                         tGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATc                                            >  2:1111978/1‑101 (MQ=255)
                                                         tGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATc                                            >  2:2109517/1‑101 (MQ=255)
                                                         tGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGAGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATc                                            <  1:207848/101‑1 (MQ=255)
                                                            cgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGG.TCGCTCCCCCCGCGGGGGCGTGGATCGa                                          >  2:2342953/4‑101 (MQ=2)
                                                               ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGG.TCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                       >  1:3052589/1‑101 (MQ=2)
                                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAg                                     >  2:1477817/1‑101 (MQ=255)
                                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAg                                     >  1:3638473/1‑101 (MQ=255)
                                                                 tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGG.TCGCTCCCCCCGCGGGGGCGTGGATCGAAActt                                     >  2:3201686/1‑99 (MQ=2)
                                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGG.TCGCTCCCCCCGCGGGGGCGTGGATCGAAActtga                                   <  2:4027893/101‑5 (MQ=2)
                                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGG.TCGCTCCCCCCGCGGGGGCGTGGATCGAAActtga                                   >  2:173144/1‑97 (MQ=2)
                                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGG.TCGCTCCCCCCGCGGGGGCGTGGATCGAAActtga                                   <  1:3028841/101‑5 (MQ=2)
                                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgcg                                  <  2:1708489/101‑1 (MQ=255)
                                                                    ccccccccGCGGGTGCGTGGATCGAAACCCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgcgc                                 <  2:2513377/99‑1 (MQ=255)
                                                                    cTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGG.TCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgat                                  <  2:3156697/101‑6 (MQ=2)
                                                                    cTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGG.TCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgat                                  <  1:2423941/101‑6 (MQ=2)
                                                                    cTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATACGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgcgc                                 <  1:2835540/101‑1 (MQ=255)
                                                                     tCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTGAGCCTC.GTATTGCGCGCT.GATCCCTCCCCCCGCGGGGGCGTGGATCGAAActtgatg                                 >  1:3615793/1‑95 (MQ=2)
                                                                     tCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGG.TCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatg                                 >  2:2942013/1‑95 (MQ=2)
                                                                      ccccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGG.TCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatga                                <  2:1577394/101‑8 (MQ=2)
                                                                      ccccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGG.TCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatga                                >  2:2148767/1‑94 (MQ=2)
                                                                      ccccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGG.TCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatga                                >  2:1368217/1‑94 (MQ=2)
                                                                      ccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGGTCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCAt                               <  1:3765577/101‑1 (MQ=255)
                                                                      ccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCAt                               <  2:2769982/101‑1 (MQ=255)
                                                                      ccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCAt                               <  1:2941958/101‑1 (MQ=255)
                                                                        ccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGG.TCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgacc                              <  1:4127583/101‑10 (MQ=2)
                                                                        ccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGG.TCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgacc                              >  2:2812426/1‑92 (MQ=2)
                                                                        ccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCt                             <  2:4195453/101‑1 (MQ=255)
                                                                         cccGCGGGGGCGTGGATCGAGACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCGCGCGGGGGCGTGGAGCGAAACAGGCGCATCTg                            >  2:36144/1‑101 (MQ=255)
                                                                         cccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTg                            <  2:358117/101‑1 (MQ=255)
                                                                         cccGAGGGGGCGTGGATCGAAACTCGGCCTCGATCC.CGTCAGCCTC.GTATTGCGCGCTGG.TCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgacca                             <  2:3052364/101‑11 (MQ=2)
                                                                          ccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGa                           <  2:660343/101‑1 (MQ=255)
                                                                          ccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGa                           <  1:2889895/101‑1 (MQ=255)
                                                                          ccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGa                           >  1:2980665/1‑101 (MQ=255)
                                                                           cgcgGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGAt                          <  1:427650/101‑1 (MQ=255)
                                                                            gcgGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATg                         >  2:2639250/1‑101 (MQ=255)
                                                                            gcgGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATg                         >  2:1268820/1‑101 (MQ=255)
                                                                             cgGGTGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGt                        <  1:669620/101‑1 (MQ=255)
                                                                             cgGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGt                        >  1:2788540/1‑101 (MQ=255)
                                                                              gggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGta                       >  2:1788324/1‑101 (MQ=255)
                                                                                gggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtata                     >  1:1300356/1‑101 (MQ=255)
                                                                                gggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtata                     >  2:2120604/1‑101 (MQ=255)
                                                                                 ggCGTGGATCTAAACTCACCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTatat                    <  2:3410193/101‑1 (MQ=255)
                                                                                  gCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATg                   <  2:3526643/101‑1 (MQ=255)
                                                                                  gCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATg                   >  2:390086/1‑101 (MQ=255)
                                                                                     atcttCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGccc                <  2:2258235/97‑1 (MQ=255)
                                                                                        aTCGAAGCTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGt             <  2:2972671/101‑1 (MQ=255)
                                                                                         tCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGcccagat            >  1:2258394/1‑97 (MQ=255)
                                                                                         tCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTg            <  1:542476/101‑1 (MQ=255)
                                                                                          cGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTga           >  2:1874824/1‑101 (MQ=255)
                                                                                          cGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTga           <  2:1948350/101‑1 (MQ=255)
                                                                                          cGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTga           >  1:1011463/1‑101 (MQ=255)
                                                                                           gAAGCTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgag          <  2:205944/101‑1 (MQ=255)
                                                                                           gAAGCTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgag          <  2:2542354/101‑1 (MQ=255)
                                                                                              aCTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACg       <  1:277032/101‑1 (MQ=255)
                                                                                               cTCGCCCTCGATCCGCGCTTGCCTCCCGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACgg      <  1:3220255/101‑1 (MQ=255)
                                                                                                tCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGc     >  1:1630622/1‑101 (MQ=255)
                                                                                                  gCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTg   >  1:1930602/1‑101 (MQ=255)
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CGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGT  >  NC_014034/1395901‑1396100

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG