Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,015 T→A 18.0% intergenic (+827/‑104) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,0150T→A18.0% 118.5 ‑0.7 0.0233intergenic (+827/‑104)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (19/23):  ref base (99/91):  total (119/114)
Fisher's exact test strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 247 total aligned reads displayed.

GGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGG  >  NC_014034/1395915‑1396115
                                                                                                    |                                                                                                        
gggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCtt                                                                                                          <  1:837335/101‑1 (MQ=255)
   cGTGGATCGAAACGACGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGcc                                                                                                       <  2:1466391/101‑1 (MQ=255)
    gTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCt                                                                                                      <  2:2442046/101‑1 (MQ=255)
    gTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCt                                                                                                      <  1:3758069/101‑1 (MQ=255)
      ggATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCa                                                                                                    <  2:2516042/101‑1 (MQ=255)
      ggATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCa                                                                                                    <  1:403791/101‑1 (MQ=255)
       gATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCAc                                                                                                   <  1:1256146/101‑1 (MQ=255)
       gATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCAc                                                                                                   >  2:1899573/1‑101 (MQ=255)
       gATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCAc                                                                                                   >  2:279452/1‑101 (MQ=255)
       gATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCAc                                                                                                   <  2:2761654/101‑1 (MQ=255)
         tCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGc                                                                                                 <  2:312702/101‑1 (MQ=255)
           gAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCTGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACCCCt                                                                                               >  1:3901147/1‑101 (MQ=255)
           gAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCt                                                                                               >  2:3810823/1‑101 (MQ=255)
            aaaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTg                                                                                              <  2:4118321/101‑1 (MQ=255)
            aaaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTg                                                                                              >  2:2935783/1‑101 (MQ=255)
              aCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGc.c                                                                                           >  1:234124/1‑101 (MQ=255)
              aCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGc.c                                                                                           >  1:3076346/1‑101 (MQ=255)
                gATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGAGCTTGCCTCACGCCTGC.cgc                                                                                         >  2:207810/1‑101 (MQ=255)
                 aTGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.cgc..g                                                                                      >  2:2241609/1‑101 (MQ=255)
                  tGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.t                                                                                    <  2:1325841/101‑1 (MQ=255)
                      ttCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCt                                                                                >  2:542412/1‑101 (MQ=255)
                       tCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTc                                                                               >  1:1669369/1‑101 (MQ=255)
                         aTGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTccc                                                                             >  1:1948476/1‑101 (MQ=255)
                             ccTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATAGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCcg                                                                         >  2:3662796/1‑101 (MQ=255)
                                aCGGCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCgg                                                                      <  1:1141378/101‑1 (MQ=255)
                                 cGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCggg                                                                     >  1:2530835/1‑101 (MQ=255)
                                    cGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGc                                                                  <  1:3592476/101‑1 (MQ=255)
                                    cGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGc                                                                  >  2:2140196/1‑101 (MQ=255)
                                     gggCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCg                                                                 >  1:1373115/1‑101 (MQ=255)
                                       gCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTg                                                               <  1:1799531/101‑1 (MQ=255)
                                        cAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..T.TCGCTCCCCCCGCGGGGGCGTgg                                                              <  2:453388/101‑1 (MQ=255)
                                        cAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTgg                                                              <  1:458481/101‑1 (MQ=255)
                                          tctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGG.TCGCTCCCCCCGCGGGGGCGTGGa                                                             <  2:3214898/94‑1 (MQ=2)
                                          gTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGAt                                                            <  1:77862/101‑1 (MQ=255)
                                          gTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGAt                                                            <  2:2334680/101‑1 (MQ=255)
                                           tGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATc                                                           >  2:1537007/1‑101 (MQ=255)
                                           tGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATc                                                           >  1:1569933/1‑101 (MQ=255)
                                           tGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATc                                                           <  1:927312/101‑1 (MQ=255)
                                           tGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGAGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATc                                                           <  1:207848/101‑1 (MQ=255)
                                            ggACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCg                                                          <  1:1699904/101‑1 (MQ=255)
                                             tcccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGG.TCGCTCCCCCCGCGGGGGCGTGGATCg                                                          <  2:2712976/97‑1 (MQ=2)
                                             gACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGa                                                         <  1:2842153/101‑1 (MQ=255)
                                             gACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGa                                                         >  2:668061/1‑101 (MQ=255)
                                               cTGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                       <  1:2367227/101‑1 (MQ=255)
                                                cggTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGG.TCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                       <  2:2875635/100‑1 (MQ=9)
                                                 ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGG.TCGCTCCCCCCTCGGGGGCCTGGATCGaaag                                                      >  1:396753/1‑100 (MQ=2)
                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGG.TCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                     <  1:505987/101‑2 (MQ=2)
                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAg                                                    >  1:3638473/1‑101 (MQ=255)
                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGG.TCGCTCCCCCCGCGGGGGCGTGGATCGAAActt                                                    >  2:3201686/1‑99 (MQ=2)
                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGG.TCGCTCCCCCCGCGGGGGCGTGGATCGAAActt                                                    <  1:3457274/101‑3 (MQ=2)
                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgg                                                   >  2:467978/1‑101 (MQ=255)
                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgg                                                   <  2:2388956/101‑1 (MQ=255)
                                                    ctcTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGG.TCGCTCCCCCCGCGGGGGCGTGGATCGAAActtg                                                   <  1:2835300/99‑4 (MQ=2)
                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGG.TCGCTCCCCCCGCGGGGGCGTGGATCGAAActtg                                                   <  1:3509242/101‑4 (MQ=2)
                                                    cGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgc                                                  <  1:3322865/101‑1 (MQ=255)
                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGG.TCGCTCCCCCCGCGGGGGCGTGGATCGAAActtga                                                  >  1:2999454/1‑97 (MQ=2)
                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgcg                                                 <  2:1708489/101‑1 (MQ=255)
                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgcg                                                 >  1:1928650/1‑101 (MQ=255)
                                                       tCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTGAGCCTC.GTATTGCGCGCT.GATCCCTCCCCCCGCGGGGGCGTGGATCGAAActtgatg                                                >  1:3615793/1‑95 (MQ=2)
                                                        ccccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGG.TCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatga                                               <  2:3972933/101‑8 (MQ=2)
                                                        ccccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGG.TCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatga                                               <  2:1577394/101‑8 (MQ=2)
                                                        ccccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGG.TCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatga                                               >  2:1368217/1‑94 (MQ=2)
                                                        ccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGGTCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCAt                                              <  1:3765577/101‑1 (MQ=255)
                                                        ccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCAt                                              <  2:2118823/101‑1 (MQ=255)
                                                        ccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCAt                                              >  1:3050810/1‑101 (MQ=255)
                                                          ccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGG.TCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgacc                                             >  2:2812426/1‑92 (MQ=2)
                                                          ccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCt                                            <  2:4195453/101‑1 (MQ=255)
                                                           cccGAGGGGGCGTGGATCGAAACTCGGCCTCGATCC.CGTCAGCCTC.GTATTGCGCGCTGG.TCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgacca                                            <  2:3052364/101‑11 (MQ=2)
                                                            ccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGa                                          <  1:2889895/101‑1 (MQ=255)
                                                             cgcgGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGAt                                         <  1:427650/101‑1 (MQ=255)
                                                               cgGGTGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGt                                       <  1:669620/101‑1 (MQ=255)
                                                                gggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGta                                      >  2:1788324/1‑101 (MQ=255)
                                                                gggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGta                                      >  2:1675095/1‑101 (MQ=255)
                                                                gggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGta                                      >  1:408324/1‑101 (MQ=255)
                                                                gggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGta                                      >  1:2246943/1‑101 (MQ=255)
                                                                  gggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtata                                    >  2:2120604/1‑101 (MQ=255)
                                                                   ggCGTGGATCTAAACTCACCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTatat                                   <  2:3410193/101‑1 (MQ=255)
                                                                   ggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTatat                                   >  2:1218742/1‑101 (MQ=255)
                                                                    gCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATg                                  <  2:3526643/101‑1 (MQ=255)
                                                                        ggATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGCCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCg                              >  1:2942613/1‑101 (MQ=255)
                                                                          aTCGAAGCTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGt                            <  2:2972671/101‑1 (MQ=255)
                                                                          aTCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGt                            >  2:1019739/1‑101 (MQ=255)
                                                                          aTCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGt                            >  1:1472090/1‑101 (MQ=255)
                                                                           tCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTg                           >  2:926712/1‑101 (MQ=255)
                                                                            cGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTga                          <  2:1948350/101‑1 (MQ=255)
                                                                            cGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTga                          >  2:1874824/1‑101 (MQ=255)
                                                                            cGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTga                          >  1:1011463/1‑101 (MQ=255)
                                                                             gAAGCTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgag                         <  2:2542354/101‑1 (MQ=255)
                                                                             gAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgag                         >  1:2099819/1‑101 (MQ=255)
                                                                                aCTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACg                      <  1:277032/101‑1 (MQ=255)
                                                                                 cTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACgg                     <  2:356649/101‑1 (MQ=255)
                                                                                   cGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCt                   <  2:3631529/101‑1 (MQ=255)
                                                                                      ccTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTc                <  2:2032638/101‑1 (MQ=255)
                                                                                        tCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGc              <  2:648351/101‑1 (MQ=255)
                                                                                         cGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCt             >  2:1078775/1‑101 (MQ=255)
                                                                                         cGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCt             >  1:380000/1‑101 (MQ=255)
                                                                                         cGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCt             <  1:1711556/101‑1 (MQ=255)
                                                                                                  cTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCcgcg    >  2:3828990/1‑101 (MQ=255)
                                                                                                  cTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCcgcg    >  2:815872/1‑101 (MQ=255)
                                                                                                    tGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGGGACGGCTGTCGCTCCCCCCGCggg  >  2:2951579/1‑101 (MQ=255)
                                                                                                    |                                                                                                        
GGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..G.TCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGG  >  NC_014034/1395915‑1396115

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG