Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,024 C→A 18.3% intergenic (+836/‑95) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,0240C→A18.3% 108.9 ‑0.9 ‑0.0229intergenic (+836/‑95)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (19/23):  ref base (99/88):  total (118/111)
Fisher's exact test strand distribution p-value = 3.97e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.98e-01

Only 100 of 253 total aligned reads displayed.

TCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGAT  >  NC_014034/1395924‑1396124
                                                                                                    |                                                                                                       
  gAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCt                                                                                                       >  2:3810823/1‑101 (MQ=255)
   aaaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTg                                                                                                      <  2:4118321/101‑1 (MQ=255)
     aCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGc.c                                                                                                   <  2:3353327/101‑1 (MQ=255)
       gATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.cgc                                                                                                 <  2:928101/101‑1 (MQ=255)
       gATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGAGCTTGCCTCACGCCTGC.cgc                                                                                                 >  2:207810/1‑101 (MQ=255)
              tCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTc                                                                                        >  1:3974919/1‑101 (MQ=255)
                aTGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTccc                                                                                      >  1:1674952/1‑101 (MQ=255)
                    ccTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCcg                                                                                  >  2:2941736/1‑101 (MQ=255)
                    ccTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATAGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCcg                                                                                  >  2:3662796/1‑101 (MQ=255)
                    ccTACGCCGGGGAGTGAACGGGCCGCGCCCCCCCCGGGGGCGTGGATCGAACCTCGCCCTCCACCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCcg                                                                                  <  1:3604957/101‑1 (MQ=255)
                     cTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCcgc                                                                                 >  2:1898425/1‑101 (MQ=255)
                      tACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCcgcg                                                                                >  2:755818/1‑101 (MQ=255)
                       aCGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCgg                                                                               >  1:1394423/1‑101 (MQ=255)
                       aCGGCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCgg                                                                               <  1:1141378/101‑1 (MQ=255)
                         gTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCgggg                                                                             >  1:3532398/1‑101 (MQ=255)
                           cGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGc                                                                           >  2:2140196/1‑101 (MQ=255)
                             ggCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCgg                                                                         >  1:1708604/1‑100 (MQ=255)
                               cAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTgg                                                                       <  1:458481/101‑1 (MQ=255)
                                 gTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                     <  1:2858544/101‑1 (MQ=255)
                                  tGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                    <  1:927312/101‑1 (MQ=255)
                                  tGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                    >  2:1537007/1‑101 (MQ=255)
                                   ggACTGGTCGCCCCCCCCGCGGGGGCGGGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                   <  1:755895/101‑1 (MQ=255)
                                      cTGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                <  1:2367227/101‑1 (MQ=255)
                                       cggTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                <  2:2875635/100‑1 (MQ=9)
                                        ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                               >  2:2475481/1‑101 (MQ=9)
                                        ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                               >  2:3457022/1‑101 (MQ=2)
                                         gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAg                                                             >  1:3638473/1‑101 (MQ=255)
                                          tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgg                                                            >  2:467978/1‑101 (MQ=255)
                                          tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgg                                                            <  2:2388956/101‑1 (MQ=255)
                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtg                                                            <  2:587475/101‑4 (MQ=2)
                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtg                                                            >  2:457321/1‑98 (MQ=2)
                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtg                                                            <  1:3677077/101‑4 (MQ=2)
                                           cGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgc                                                           <  1:3322865/101‑1 (MQ=255)
                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgcg                                                          <  2:1708489/101‑1 (MQ=255)
                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgcg                                                          >  1:1928650/1‑101 (MQ=255)
                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgcg                                                          >  2:283282/1‑101 (MQ=255)
                                             cTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgat                                                          <  2:2329741/101‑6 (MQ=2)
                                             cTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATACGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgcgc                                                         <  1:2835540/101‑1 (MQ=255)
                                              tCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatg                                                         >  1:2561823/1‑95 (MQ=2)
                                               ccccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatga                                                        <  1:1786312/101‑8 (MQ=2)
                                               ccccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatga                                                        <  2:1577394/101‑8 (MQ=2)
                                               ccccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatga                                                        >  2:4179936/1‑94 (MQ=2)
                                               ccccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatga                                                        <  2:3972933/101‑8 (MQ=2)
                                               ccccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatga                                                        >  2:1368217/1‑94 (MQ=2)
                                               ccccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatga                                                        <  1:3038428/101‑8 (MQ=2)
                                               ccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCAt                                                       <  2:2769982/101‑1 (MQ=255)
                                               ccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCAt                                                       <  2:2118823/101‑1 (MQ=255)
                                               ccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCAt                                                       <  1:2941958/101‑1 (MQ=255)
                                                cccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATc                                                      >  1:2092368/1‑101 (MQ=255)
                                                cccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATc                                                      <  2:3112689/101‑1 (MQ=255)
                                                 ccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgacc                                                      <  1:4127583/101‑10 (MQ=2)
                                                 ccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCt                                                     <  1:1354204/101‑1 (MQ=255)
                                                     gcgGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATg                                                 >  2:2639250/1‑101 (MQ=255)
                                                     gcgGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATg                                                 >  2:1268820/1‑101 (MQ=255)
                                                     gcgGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATg                                                 <  1:2267001/101‑1 (MQ=255)
                                                       gggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGta                                               >  2:1675095/1‑101 (MQ=255)
                                                       gggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGta                                               >  1:2246943/1‑101 (MQ=255)
                                                       gggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGta                                               >  2:1788324/1‑101 (MQ=255)
                                                       gggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGta                                               >  1:1541943/1‑101 (MQ=255)
                                                        ggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtat                                              <  2:2730765/101‑1 (MQ=255)
                                                         gggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtata                                             >  2:2120604/1‑101 (MQ=255)
                                                          ggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTatat                                            >  2:1218742/1‑101 (MQ=255)
                                                                  tCGAACCTCGCCCTCGATCCGCGCTTGCCTCACCCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTg                                    <  2:1031530/101‑1 (MQ=255)
                                                                  tCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTg                                    <  1:542476/101‑1 (MQ=255)
                                                                   cGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTga                                   <  2:1948350/101‑1 (MQ=255)
                                                                   cGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTga                                   >  2:1874824/1‑101 (MQ=255)
                                                                   cGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTga                                   >  1:1011463/1‑101 (MQ=255)
                                                                   cGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCCGTga                                   <  1:3783396/101‑1 (MQ=255)
                                                                    gAAGCTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgag                                  <  2:2542354/101‑1 (MQ=255)
                                                                    gAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgag                                  >  1:2099819/1‑101 (MQ=255)
                                                                       aCTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACg                               <  1:277032/101‑1 (MQ=255)
                                                                       aCTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACg                               >  2:2998303/1‑101 (MQ=255)
                                                                        cTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACgg                              <  2:356649/101‑1 (MQ=255)
                                                                         tCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGc                             >  1:1630622/1‑101 (MQ=255)
                                                                          cGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCt                            <  2:3631529/101‑1 (MQ=255)
                                                                           gCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTg                           >  2:3201504/1‑101 (MQ=255)
                                                                            cccTCGATCCCCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGt                          <  1:193369/101‑1 (MQ=255)
                                                                             ccTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTc                         <  1:477701/101‑1 (MQ=255)
                                                                             ccTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTc                         <  2:2032638/101‑1 (MQ=255)
                                                                               tCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGc                       <  2:648351/101‑1 (MQ=255)
                                                                                   tCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTccc                   >  1:3959184/1‑101 (MQ=255)
                                                                                   tCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTccc                   <  1:867209/101‑1 (MQ=255)
                                                                                        gcTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCcgc              >  2:3204801/1‑101 (MQ=255)
                                                                                         cTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCcgcg             >  2:3828990/1‑101 (MQ=255)
                                                                                         cTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCcgcg             >  2:815872/1‑101 (MQ=255)
                                                                                           tGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGGGACGGCTGTCGCTCCCCCCGCggg           >  2:2951579/1‑101 (MQ=255)
                                                                                           tGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCggg           >  1:3481115/1‑101 (MQ=255)
                                                                                              cTCGCGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCTCGGGGGc        <  2:2210268/101‑1 (MQ=255)
                                                                                              cTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGc        <  1:381522/101‑1 (MQ=255)
                                                                                              cTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGc        >  1:1822801/1‑101 (MQ=255)
                                                                                              cTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGc        >  2:1018930/1‑101 (MQ=255)
                                                                                               tcccGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCg       <  1:3023269/98‑1 (MQ=255)
                                                                                               tCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCg       <  2:1394324/101‑1 (MQ=255)
                                                                                                cACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGt      <  1:2952314/101‑1 (MQ=255)
                                                                                                cACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGt      >  2:115725/1‑101 (MQ=255)
                                                                                                cACGCCTGC.CGC..GTCGCTCCCCACGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCgg      >  2:3921998/1‑100 (MQ=255)
                                                                                                  cGCCGGC.CGC..GTCGCTCCCCCCGCGGGCGCGTGGATCGAAGCAGGCTCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTgg    <  1:36159/101‑1 (MQ=38)
                                                                                                   gCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGGGGa   >  1:31307/1‑101 (MQ=255)
                                                                                                    ccTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGAt  >  1:3072382/1‑101 (MQ=255)
                                                                                                    ccTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGAt  <  1:4226280/101‑1 (MQ=255)
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TCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGAT  >  NC_014034/1395924‑1396124

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG