Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,154 G→A 2.2% A12A (GCG→GTA RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,1540G→A 2.2% 3.3 ‑2.1 ‑0.9183A12A (GCG→GCARCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (0/4):  ref base (91/88):  total (91/92)
Fisher's exact test strand distribution p-value = 1.21e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.27e-01

Only 100 of 225 total aligned reads displayed.

GGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGC  >  NC_014034/1396054‑1396254
                                                                                                      |                                                                                                    
ggATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTcgcg                                                                                                      <  2:1653784/101‑1 (MQ=255)
ggATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTcgcg                                                                                                      >  1:1993935/1‑101 (MQ=255)
 gATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCgcgc                                                                                                     >  1:3390401/1‑101 (MQ=255)
   tCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGcgccg                                                                                                   >  1:1841090/1‑101 (MQ=255)
    cGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGcgccgc                                                                                                  >  1:3207625/1‑101 (MQ=255)
    cGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGcgccgc                                                                                                  >  2:425001/1‑101 (MQ=255)
     gAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCgccccc                                                                                                 >  1:2558891/1‑101 (MQ=255)
          aGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCt                                                                                            <  1:4084753/101‑1 (MQ=255)
          aGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCCCG.CGCCAccgcg                                                                                           >  2:1501876/1‑97 (MQ=38)
               cATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTc                                                                                       <  2:1674837/101‑1 (MQ=255)
                    gATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTcc                                                                                  <  2:2099681/101‑1 (MQ=255)
                     aTGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTccc                                                                                 <  1:1558965/101‑1 (MQ=255)
                     aTGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGGCGCTccc                                                                                 >  2:2080258/1‑101 (MQ=255)
                       gTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTccccc                                                                               <  1:425058/101‑1 (MQ=255)
                       gTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTccccc                                                                               <  2:3207386/101‑1 (MQ=255)
                        tataTGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTcccccc                                                                              <  2:1630513/101‑1 (MQ=255)
                        tataTGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTcccccc                                                                              <  1:2905089/101‑1 (MQ=255)
                        tataTGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTcccccc                                                                              >  1:2255804/1‑101 (MQ=255)
                        tataTGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTcccccc                                                                              <  1:115749/101‑1 (MQ=255)
                        tataTGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTcccccc                                                                              <  1:1268916/101‑1 (MQ=255)
                        tataTGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTcccccc                                                                              <  1:1675210/101‑1 (MQ=255)
                           atGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCcgcg                                                                           >  1:3079589/1‑101 (MQ=255)
                            tGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCCCgg                                                                          >  1:4162705/1‑101 (MQ=255)
                             gCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACCCCGCGTCCCCCCCCCCCCggg                                                                         >  1:3021265/1‑101 (MQ=37)
                              cccGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCgggg                                                                        <  2:3076116/101‑1 (MQ=255)
                                cGGTGGGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGc                                                                      <  2:38855/101‑1 (MQ=255)
                                cGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGCC.ATCTGT.CAGGCTCGTGCCCGCACGCTTCGTCGCTCCCCCCGCggggcc                                                                      >  1:1789086/1‑99 (MQ=25)
                                   tgtctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCGGTCAGCCTCGTATTG.CGCGCTG.GTCGCTCCCCCCGCGGGGGCGTg                                                                   <  1:680080/91‑1 (MQ=9)
                                   tGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTg                                                                   >  2:1866945/1‑101 (MQ=255)
                                   tGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTg                                                                   <  1:2998525/101‑1 (MQ=255)
                                   tGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTg                                                                   <  2:1928524/101‑1 (MQ=255)
                                   tGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTg                                                                   >  2:2166172/1‑101 (MQ=255)
                                   tGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTg                                                                   <  1:1086573/101‑1 (MQ=255)
                                   tGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTg                                                                   <  1:639257/101‑1 (MQ=255)
                                    gagaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCGCTGCGTCGCTCCCCCCGCGGGGGCGTgg                                                                  >  2:1127043/1‑101 (MQ=255)
                                    gagaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTgg                                                                  >  2:1086479/1‑101 (MQ=255)
                                    gagaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTgg                                                                  <  1:2714707/101‑1 (MQ=255)
                                    gagaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTgg                                                                  >  2:4164299/1‑101 (MQ=255)
                                      gaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                <  2:2558700/101‑1 (MQ=255)
                                      gaCGGCTGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                <  2:819677/101‑1 (MQ=255)
                                       aCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGGTc                                                               >  1:3314737/1‑101 (MQ=255)
                                       aCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                               <  1:354119/101‑1 (MQ=255)
                                          gCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                            <  1:1396583/101‑1 (MQ=255)
                                          gCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGGGGATCGaa                                                            >  2:925328/1‑101 (MQ=255)
                                          gCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCCGCGTCCCTCCCCCCGCGGGGGCGTGGATCGaa                                                            >  1:4123816/1‑101 (MQ=255)
                                           cTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                           >  2:4053027/1‑101 (MQ=255)
                                            tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGGGGGGATCGAAAc                                                          >  2:415683/1‑101 (MQ=255)
                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                         >  2:695504/1‑101 (MQ=255)
                                             gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                         <  1:974321/101‑1 (MQ=255)
                                              tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCCCGTCGCCCCGCTGCGCCGCTCCCCCCTCGGGGGCGTGGATCGGCAccc                                                        >  2:3305613/1‑101 (MQ=37)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCg                                                       >  1:1340661/1‑101 (MQ=255)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCg                                                       >  1:3806195/1‑101 (MQ=255)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCgg                                                      <  2:510773/101‑1 (MQ=255)
                                                  tCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCATCCTGGGTCGCTCCCCCCCCGGGGGCGTGGATCGAAACCCGGCg                                                    >  2:2074468/1‑101 (MQ=38)
                                                  tCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACCCGGCg                                                    >  1:3454312/1‑101 (MQ=255)
                                                   ccccccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGa                                                   >  2:1176414/1‑101 (MQ=255)
                                                    cccccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAAACCGGCGaa                                                  >  2:211554/1‑101 (MQ=255)
                                                     ccccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGaaa                                                 <  1:1768591/101‑1 (MQ=255)
                                                     ccccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCCCGGGGGCGTGGATCGAAACCCGGCGaaa                                                 >  2:1406281/1‑101 (MQ=255)
                                                        cgcgGGGGCGTGGATTGAAACTTTTCGGC.ATCTGG.CAGGCCCGCGCCGCCACGCCGGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAAc                                              <  2:2835161/101‑1 (MQ=37)
                                                        cgcgGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAAc                                              <  2:2727225/101‑1 (MQ=255)
                                                          cgGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACgg                                            >  2:2806614/1‑101 (MQ=255)
                                                          cgGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACgg                                            >  2:3471234/1‑101 (MQ=255)
                                                          cgGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACgg                                            <  1:1642629/101‑1 (MQ=255)
                                                          cgGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACgg                                            <  1:1020445/101‑1 (MQ=255)
                                                           gggggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCTCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAACACGGc                                           >  2:3790591/1‑101 (MQ=255)
                                                           gggggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGc                                           <  1:2320054/101‑1 (MQ=255)
                                                              ggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGCAACCCGGCGGAAAAAGGCCAt                                        >  1:2078549/1‑101 (MQ=255)
                                                              ggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCAt                                        <  2:3332388/101‑1 (MQ=255)
                                                              ggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCAt                                        <  2:3617378/101‑1 (MQ=255)
                                                              ggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCAt                                        <  1:2130753/101‑1 (MQ=255)
                                                              ggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCAt                                        >  2:2710934/1‑101 (MQ=255)
                                                                  tGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATCccc                                    >  1:806790/1‑100 (MQ=255)
                                                                   ggATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTc                                   >  2:3229300/1‑101 (MQ=255)
                                                                    gATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTcc                                  >  1:51778/1‑101 (MQ=255)
                                                                    gATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTcc                                  >  1:3960247/1‑101 (MQ=255)
                                                                    gATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTcc                                  >  2:3710461/1‑101 (MQ=255)
                                                                     aTCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACCCGGCGGAAAACGGCCATGCCTCCg                                 >  2:3688000/1‑101 (MQ=255)
                                                                     aTCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCg                                 <  1:2639456/101‑1 (MQ=255)
                                                                      tCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCgg                                >  1:3683468/1‑101 (MQ=255)
                                                                       cGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCggg                               >  1:4010943/1‑101 (MQ=255)
                                                                        gAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGgt                              <  2:2255645/101‑1 (MQ=255)
                                                                            cTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCt                          <  2:3439199/101‑1 (MQ=255)
                                                                            cTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACCCTGCGTCGCTCCCCCCGCGGGGGCCTGGATCGGAACCCGGCGAAAAAAGGCCCTGCCTCCGGGCGTcg                          >  2:2385449/1‑100 (MQ=37)
                                                                              tttCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTgg                        <  2:1569824/101‑1 (MQ=255)
                                                                               ttCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCCGGc                       <  2:3582781/101‑1 (MQ=255)
                                                                                 cGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCAAAAAACGGCCATGCCTCCGGGGGTCTGGCTg                     >  2:178666/1‑101 (MQ=255)
                                                                                   gc.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTc                   <  1:1021087/100‑1 (MQ=255)
                                                                                    c.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCg                  <  2:2308524/101‑1 (MQ=255)
                                                                                      aTCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGc                 <  1:3305854/101‑1 (MQ=255)
                                                                                       tCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAAAGACCAAACCACCGGGTATCTGGCTGTCCCt                >  1:1920646/1‑101 (MQ=25)
                                                                                       tCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCt                <  1:777736/101‑1 (MQ=255)
                                                                                         tGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcc              <  1:3922258/101‑1 (MQ=255)
                                                                                             cAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTccccc           >  2:3084087/1‑101 (MQ=255)
                                                                                             cAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTccccc           >  2:2321764/1‑101 (MQ=255)
                                                                                             cAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATAGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTccccc           >  2:844814/1‑101 (MQ=255)
                                                                                              aGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCGTGCCTCCGGGGGTCTGGCTGTCGCTcccccc          >  2:3954139/1‑101 (MQ=255)
                                                                                              aGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcccccc          <  1:2806833/101‑1 (MQ=255)
                                                                                              aGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcccccc          <  2:1340561/101‑1 (MQ=255)
                                                                                                      gccgccACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGc  >  1:2311419/1‑101 (MQ=255)
                                                                                                      |                                                                                                    
GGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGC  >  NC_014034/1396054‑1396254

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG